Automated Blood Coagulation Analyzer CA-500

Basic Data Sheet -(Factors)

Extrinsic Factor (Factor VII)

1) Standard curve
Intrinsic Factor (Factor VIII)
2) Standard curve
 To readers of this scientific data

Due to measurement conditions and nature of the speci

* men, different results from this sheet might be observed.

Sysmex corporation
Scientific division
4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271 JAPAN
Tel : 078-991-1911
Fax : 078-991-1917
E-mail address : scientific@sysmex.co.jp

1
 CA-500

1)-1 Standard Curve - (F.VII) using Thromborel S

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 135.0 90.0 45.0 22.5 11.2
Clotting time (sec) 23.20 26.30 34.35 43.75 56.95

LogCurve

Method < Instrument > CA-500

< Reagent > (1) Thromborel S Lot No. 505426
(2) Clotting Factor-VII Deficient Plasma Lot No. 500762C
Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VII Activity was set up, using Thromborel S, Clotting Factor-VII
Deficient Plasma and Standard Human plasma. Good standard curve was provided with the plas
ma activity set at 90%.

2
 CA-500

1)-2 Standard Curve - (F.VII) using Thromboplastin•C Plus

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 135.0 90.0 45.0 22.5 11.2
Clotting time (sec) 18.40 20.35 25.40 31.50 39.00

LogCurve

Method < Instrument > CA-500

< Reagent > (1) Thromboplastin•C Plus Lot No. TPC-416
(2) Clotting Factor-VII Deficient Plasma Lot No. 500762C

Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VII Activity was set up, using Thromboplastin•C Plus, Clotting
Factor-VII Deficient Plasma and Standard Human plasma. Good standard curve was provided
with the plasma activity set at 90%.

3
 CA-500

1)-3 Standard Curve - (F.VII) using INNOVIN

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 135.0 90.0 45.0 22.5 11.2
Clotting time (sec) 18.70 21.50 28.95 39.7 55.75

LogCurve

Method < Instrument > CA-500

< Reagent > (1) Innovin Lot No. TFS-211
(2) Clotting Factor-VII Deficient Plasma Lot No. 500762C
Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VII Activity was set up, using Innovin, Clotting Factor-VII Defi
cient Plasma and Standard Human plasma. Good standard curve was provided with the plasma
activity set at 90%.

4
 CA-500

2)-1 Standard Curve - (F.VIII) using Pathromtin SL

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 138.0 92.0 46.0 23.0 11.5
Clotting time (sec) 64.40 69.00 77.80 89.35 100.80

LogCurve

Method < Instrument > CA-500

< Reagent > (1) Pathromtin SL Lot No. 523737
(2) Clotting Factor-VIII Deficient Plasma Lot No. 503841
(3) Calcium Chloride - 0.025mol/L Lot No. 506871
Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VIII Activity was set up, using Pathromtin SL, Clotting Factor-
VIII Deficient Plasma and Standard Human plasma. Good standard curve was provided with the
plasma activity set at 92%.

5
 CA-500

2)-2 Standard Curve - (F.VIII) using ACTIN

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 138.0 92.0 46.0 23.0 11.5
Clotting time (sec) 49.60 53.55 62.80 71.30 80.30

LogCurve

Method < Instrument > CA-500

< Reagent > (1) ACTIN Lot No. 527111
(2) Clotting Factor-VIII Deficient Plasma Lot No. 503841
(3) Calcium Chloride - 0.025mol/L Lot No. 506871
Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VIII Activity was set up, using ACTIN, Clotting Factor-VIII Defi
cient Plasma and Standard Human plasma. Good standard curve was provided with the plasma
activity set at 92%.

6
 CA-500

2)-3 Standard Curve - (F.VIII) using ACTIN FS

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 138.0 92.0 46.0 23.0 11.5
Clotting time (sec) 61.10 65.75 74.95 85.15 95.45

LogCurve

Method < Instrument > CA-500

< Reagent > (1) ACTIN FS Lot No. 527205
(2) Clotting Factor-VIII Deficient Plasma Lot No. 503841
(3) Calcium Chloride - 0.025mol/L Lot No. 506871
Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VIII Activity was set up, using ACTIN FS, Clotting Factor-VIII
Deficient Plasma and Standard Human plasma. Good standard curve was provided with the plas
ma activity set at 92%.

7
 CA-500

2)-4 Standard Curve - (F.VIII) using ACTIN FSL

Results

Dilution ratio 3/2 1/1 1/2 1/4 1/8

Activity (%) 138.0 92.0 46.0 23.0 11.5
Clotting time (sec) 78.05 83.25 93.70 104.85 116.20

LogCurve

Method < Instrument > CA-500

< Reagent > (1) ACTIN FSL Lot No. FSL-211
(2) Clotting Factor-VIII Deficient Plasma Lot No. 503841
(3) Calcium Chloride - 0.025mol/L Lot No. 506871
Specimens Standard Human Plasma Lot No. 502568B

Dilution Owren's veronal buffer

Comment
The standard curve of Factor VIII Activity was set up, using ACTIN FSL, Clotting Factor-VIII
Deficient Plasma and Standard Human plasma. Good standard curve was provided with the plas
ma activity set at 92%.