GRADIENT PERFORMANCE REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 100 Gradient Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Gradient PM Sample Kit 1 Lot Number: N070015
Report Method Name: Gradient Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 12042 DB ID:
Result 847558692
Set Id 12277
Sample Set Name: Gradient Sample Set Start Date: 11/5/2010 1:28:20 PM

The Gradient Performance qualification injects six replicates of a seven component mixture. The components are
separated with a four minute gradient using Acetonitrile in the B Pump.

The standard deviation of the Retention Time for each component is reported for all six injections.
The qualification passes if the SD for the retention time for all seven components is < or = 1.0 seconds.

Gradient Performance Overall Test Result

Retention Time (Seconds) Retention Time (Seconds)

Retention Time (Seconds)
Name Standard Deviation Standard Deviation
Standard Deviation
Specification Pass Fail
1 2-acetylfuran SD RT < or = 1.0sec 0.1 PASS
2 acetanilide SD RT < or = 1.0sec 0.1 PASS
3 acetophenone SD RT < or = 1.0sec 0.1 PASS
4 propiophenone SD RT < or = 1.0sec 0.1 PASS
5 butylparaben SD RT < or = 1.0sec 0.1 PASS
6 benzophenone SD RT < or = 1.0sec 0.1 PASS
7 valerophenone SD RT < or = 1.0sec 0.1 PASS

Printed 8:25:22 AM US/Eastern 11/17/2010 Page: 1 of 3

 GRADIENT PERFORMANCE REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 100 Gradient Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Gradient PM Sample Kit 1 Lot Number: N070015
Report Method Name: Gradient Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 12042 DB ID:
Result 847558692
Set Id 12277
Sample Set Name: Gradient Sample Set Start Date: 11/5/2010 1:28:20 PM

Gradient Performance Summary Results

Component Summary For A_RTsec

Result
SampleName Inj Vial 2-acetylfuran acetanilide acetophenone propiophenone butylparaben benzophenone v alerophenone
Id
1 Gradient A1B1 1 11 12286 39.18 47.34 72.38 94.10 106.74 118.62 127.45

2 Gradient A1B1 2 11 12287 39.15 47.29 72.32 94.04 106.69 118.57 127.40

3 Gradient A1B1 3 11 12288 39.02 47.17 72.25 93.98 106.63 118.51 127.37

4 Gradient A1B1 4 11 12289 39.06 47.18 72.21 93.93 106.57 118.44 127.30

5 Gradient A1B1 5 11 12290 38.98 47.11 72.19 93.92 106.57 118.45 127.34

6 Gradient A1B1 6 11 12291 39.05 47.16 72.17 93.88 106.50 118.38 127.29

Mean 39 47 72 94 107 118 127

Std. Dev. 0.08 0.09 0.08 0.08 0.09 0.09 0.06

Printed 8:25:22 AM US/Eastern 11/17/2010 Page: 2 of 3

 GRADIENT PERFORMANCE REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 100 Gradient Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Gradient PM Sample Kit 1 Lot Number: N070015
Report Method Name: Gradient Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 12042 DB ID:
Result 847558692
Set Id 12277
Sample Set Name: Gradient Sample Set Start Date: 11/5/2010 1:28:20 PM

Gradient Performance Chromatogram Overlay

acetanilide

0.12
butylparaben

benzophenone

0.10
acetophenone
2-acetylfuran

0.08

0.06
propiophenone
AU

0.04
valerophenone

0.02

0.00

-0.02

-0.04

0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00

Minutes
SampleName Gradient A1B1; Injection 1
SampleName Gradient A1B1; Injection 2
SampleName Gradient A1B1; Injection 3
SampleName Gradient A1B1; Injection 4
SampleName Gradient A1B1; Injection 5
SampleName Gradient A1B1; Injection 6

Printed 8:25:22 AM US/Eastern 11/17/2010 Page: 3 of 3