GRADIENT PERFORMANCE REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: Del_Patrol2 Acquired By: Jennb

Project Name: SQT_PATrol_TUV_CM_rev C Column serial Number: 11581505610K
Sample Set Method: 100 Gradient Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Gradient PM Sample Kit 1 Lot Number: N070015
Report Method Name: Gradient Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11042 DB ID:
Result 847558692
Set Id 11777
Sample Set Name: Gradient Sample Set Start Date: 10/25/2010 11:37:54 PM

The Gradient Performance qualification injects six replicates of a seven component mixture. The components are
separated with a four minute gradient using Acetonitrile in the B Pump.

The standard deviation of the Retention Time for each component is reported for all six injections.
The qualification passes if the SD for the retention time for all seven components is < or = 1.0 seconds.

Gradient Performance Overall Test Result

Retention Time (Seconds) Retention Time (Seconds)

Retention Time (Seconds)
Name Standard Deviation Standard Deviation
Standard Deviation
Specification Pass Fail
1 2-acetylfuran SD RT < or = 1.0sec 0.0 PASS
2 acetanilide SD RT < or = 1.0sec 0.0 PASS
3 acetophenone SD RT < or = 1.0sec 0.1 PASS
4 propiophenone SD RT < or = 1.0sec 0.1 PASS
5 butylparaben SD RT < or = 1.0sec 0.1 PASS
6 benzophenone SD RT < or = 1.0sec 0.1 PASS
7 valerophenone SD RT < or = 1.0sec 0.1 PASS

Printed 8:39:18 AM US/Eastern 11/17/2010 Page: 1 of 3

 GRADIENT PERFORMANCE REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: Del_Patrol2 Acquired By: Jennb

Project Name: SQT_PATrol_TUV_CM_rev C Column serial Number: 11581505610K
Sample Set Method: 100 Gradient Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Gradient PM Sample Kit 1 Lot Number: N070015
Report Method Name: Gradient Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11042 DB ID:
Result 847558692
Set Id 11777
Sample Set Name: Gradient Sample Set Start Date: 10/25/2010 11:37:54 PM

Gradient Performance Summary Results

Component Summary For A_RTsec

Result
SampleName Inj Vial 2-acetylfuran acetanilide acetophenone propiophenone butylparaben benzophenone v alerophenone
Id
1 Gradient A1B1 1 11 11786 37.50 44.90 69.52 91.35 103.99 115.88 124.77

2 Gradient A1B1 2 11 11787 37.42 44.83 69.43 91.11 103.74 115.65 124.55

3 Gradient A1B1 3 11 11788 37.46 44.86 69.42 91.15 103.73 115.65 124.55

4 Gradient A1B1 4 11 11789 37.40 44.79 69.34 91.02 103.65 115.57 124.49

5 Gradient A1B1 5 11 11790 37.41 44.80 69.35 91.03 103.66 115.55 124.48

6 Gradient A1B1 6 11 11791 37.40 44.82 69.39 90.97 103.64 115.56 124.51

Mean 37 45 69 91 104 116 125

Std. Dev. 0.04 0.04 0.07 0.13 0.13 0.13 0.11

Printed 8:39:18 AM US/Eastern 11/17/2010 Page: 2 of 3

 GRADIENT PERFORMANCE REPORT

AC Q U I SI TI O N I N F O R M ATI O N

System Name: Del_Patrol2 Acquired By: Jennb

Project Name: SQT_PATrol_TUV_CM_rev C Column serial Number: 11581505610K
Sample Set Method: 100 Gradient Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Gradient PM Sample Kit 1 Lot Number: N070015
Report Method Name: Gradient Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11042 DB ID:
Result 847558692
Set Id 11777
Sample Set Name: Gradient Sample Set Start Date: 10/25/2010 11:37:54 PM

Gradient Performance Chromatogram Overlay

acetanilide

butylparaben

0.12
benzophenone
2-acetylfuran

0.10
acetophenone

propiophenone

0.08
valerophenone
AU

0.06

0.04

0.02

0.00

0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00

Minutes
SampleName Gradient A1B1; Injection 1
SampleName Gradient A1B1; Injection 2
SampleName Gradient A1B1; Injection 3
SampleName Gradient A1B1; Injection 4
SampleName Gradient A1B1; Injection 5
SampleName Gradient A1B1; Injection 6

Printed 8:39:18 AM US/Eastern 11/17/2010 Page: 3 of 3