EXCITATION WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: e2695_2475 MSG

Project Path: RC2_SQT _e2695_2475 final
Processing Method: 040 EX Wavelength Acc PM
Sample Kit Expiration Date: Friday, August 10, 2018 12:00:00 AM EDT
Sample Kit Lot Number: W17111613
Report Method: 040 EX Wavelength Acc RM Acquired By: Melissa
Sample Set Name: EX Wavelength Acc SSM Column Serial Number: 01833630013817
Sample Set Id: 7928 Result Set Id: 7950

The Excitation Wavelength Accuracy test uses the Rayleigh scattering process of the emission wavelength to verify
accuracy of the detector.

Emission wavelength is set to 700 nm and the excitation wavelength is changed in 1nm increments from 343 nm
to 357 nm to measure the second order Rayleigh scattering band.

The Excitation Wavelength Accuracy test passes only when all 15 expected peaks are identified, and the
wavelength associated with the highest peak is 350 nm + or - 6 nm.

Excitation Wav elength Accuracy Results

Excitation W avelength Excitation W avelength Excitation W avelength
Specification Lambda max (nm) Pass/Fail
1 350 + or - 6nm 352 PASS

Waters Project Name and Version: SQT_E_2695_2475 v4.00 © 2007 - 2017 Waters Corporation. All rights reserved.
Date: Monday, February 20, 2017
Printed: 11:05:50 AM US/Eastern Page: 1 of 2
 EXCITATION WAVELENGTH ACCURACY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: e2695_2475 MSG Sample Set Id: 7928

Project Path: RC2_SQT_e2695_2475 final Result Set Id: 7950

Excitation Wav elength Scan

350.00

352nm - 6.979
351nm - 6.557

353nm - 7.582
350nm - 5.825

354nm - 8.322
349nm - 5.317
348nm - 4.717
300.00

355nm - 8.742
347nm - 4.134
346nm - 3.531
345nm - 2.946

356nm - 9.340
250.00

357nm - 9.944
344nm - 2.394

200.00
343nm - 1.891
EU

150.00

100.00

50.00

0.00

0.00 2.00 4.00 6.00 8.00 10.00 12.00

Minutes

Excitation Wav elength Scan Results

Excitation Height
Channel Name Result Id
Wavelength (µV)
1 352nm 3578880.07 2475ChA ex330/em700 7968
2 351nm 3576563.05 2475ChA ex330/em700 7968
3 353nm 3548942.50 2475ChA ex330/em700 7968
4 350nm 3538573.66 2475ChA ex330/em700 7968
5 354nm 3488972.03 2475ChA ex330/em700 7968
6 349nm 3473363.52 2475ChA ex330/em700 7968
7 348nm 3371956.61 2475ChA ex330/em700 7968
8 355nm 3325148.81 2475ChA ex330/em700 7968
9 347nm 3249456.04 2475ChA ex330/em700 7968
10 346nm 3088625.18 2475ChA ex330/em700 7968
11 345nm 2912269.42 2475ChA ex330/em700 7968
12 356nm 2901105.46 2475ChA ex330/em700 7968
13 357nm 2580352.22 2475ChA ex330/em700 7968
14 344nm 2486270.65 2475ChA ex330/em700 7968
15 343nm 2287324.55 2475ChA ex330/em700 7968

Waters Project Name and Version: SQT_E_2695_2475 v4.00 © 2007 - 2017 Waters Corporation. All rights reserved.
Date: Monday, February 20, 2017
Printed: 11:05:50 AM US/Eastern Page: 2 of 2