UV TEST INJECTIONS REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Acquired By: ryan

Processing Method: MS Test Injections PM, UV Test Sample Kit 2 Lot Number: 1245
Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Sample Kit 2 Exp. Date: 12/22/2014 12:00:00 AM
Sample Set Id: 5051 Column Serial Number: W1412341AD
Sample Set Name: Test Injections SSM Result Set Id: 5057
Sample Set Start Date: 1/6/2015 4:21:06 PM EST
TE S T D E S C R I P TI O N
Test injections are performed to ensure that the system is equilibrated and prepared for qualification.
The system makes replicate injections of a caffeine solution at the same concentration and injection
volume. The standard deviation of the retention time and the %RSD for Area and Height should be
minimal in a well-functioning system.

The system is ready to be qualified if the Area %RSD is < or = to 1.0 the Height %RSD is < or = 1.5
the Retention Time SD < or = 1.5 seconds and the Minimum Height is > or = 1.80AU.

Caffeine Area and Height Results

Area Height
Area Spec Area (% RSD) Height Spec Height (% RSD)
System Status System Status
1 %RSD < or = 1.0 0.100 System Ready %RSD < or = 1.5 0.16 System Ready

Caffeine Minimum Height Results

Minimum Height Minimum Height
Height (AU)
Spec System Status
1 Height > or = 1.8 AU 1.868 System Ready

Caffeine Retention Time Results

Retention Time Retention Time
Retention Time Spec
Standard Deviation (sec) System Status
1 SD RT < or = 1.5 sec 0.24 System Ready

Printed 8:22:08 AM US/Eastern 1/7/2015 Page: 1 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 UV TEST INJECTIONS REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 5051

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 5057

Printed 8:22:08 AM US/Eastern 1/7/2015 Page: 2 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 UV TEST INJECTIONS REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 5051

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 5057

Caffeine Ov erlaid Chromatograms

1.80

1.60

1.40

1.20

1.00
AU

0.80

0.60
Caffeine - 0.729

0.40

0.20

0.00

0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00
Minutes
SampleName Caffeine; Injection 1
SampleName Caffeine; Injection 2
SampleName Caffeine; Injection 3

Printed 8:22:08 AM US/Eastern 1/7/2015 Page: 3 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 UV TEST INJECTIONS REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 5051

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 5057

Chromatographic Peak Results

Caffeine Solution
Retention Time Retention Time Height Points
Inj ection Name Area Result Id
(min) (sec) (µV) Across Peak
1 1 Caffeine 0.729 43.77 8790305.804 1868057.63 90 5097
2 2 Caffeine 0.735 44.12 8778995.717 1868612.78 91 5098
3 3 Caffeine 0.737 44.23 8796276.072 1863058.74 91 5099

Caffeine Summary Results

Retention Time (sec)
Area (% RSD) Height (% RSD) Height (AU)
Standard Deviation
1 0.24 0.10 0.16 1.87

Printed 8:22:08 AM US/Eastern 1/7/2015 Page: 4 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00