LC Calibration Report

Processing Method: scopoletin_ed System: FLR Alliance

Processing Method ID: 1476 Channel: 2475ChA ex360/em450
Calibration ID: 1479 Proc. Chnl. Descr.: 2475ChA ex360/em450
Date Calibrated: 12/03/2019 12:53:19 WIT

3.0x109

2.5x109

2.0x109

1.5x109
Area

1.0x109

5.0x108

0.0

-5.0x108
0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50
Amount
Peak Name: Scopoletin; RT: 30.000; Fit Type: Linear (1st Order); Cal Curve Id: 1480; R: 0.999635; R^2:
0.999269; Weighting: None; Equation: Y = 5.46e+009 X - 1.01e+007; Normalized Intercept/Slope: -0.007395;
RSD(E): 5.273071

Peak: Scopoletin
Calc. %
Sample Name Result Id Peak Name Level X Value Response Manual Ignore
Value Deviation
1 std scopoletin 2 1482 Scopoletin 1 0.001 250109.382 0.002 89.74 No No
2 std scopoletin 1 1481 Scopoletin 2 0.002 37902627.564 0.009 251.67 No No
3 std scopoletin 3 1483 Scopoletin 3 0.005 287136939.486 No Yes
4 std scopoletin 4 1487 Scopoletin 4 0.100 487429859.603 0.091 -8.94 No No
5 std scopoletin 5 1488 Scopoletin 5 0.250 1727528776.518 No Yes
6 std scopoletin 6 1490 Scopoletin 6 0.500 2730442297.237 0.502 0.35 No No

Reported by User: System Project Name: Scopoletin - FLD

Report Method: LC Calibration Report Date Printed:
Report Method ID: 1003
1003 12/03/2019
Page: 1 of 1 12:53:33 Asia/Jakarta
 Multi Sample Summary
SAMPLE INFORMATION
Sample Name: std scopoletin 4, std scopoletin 1, Acquired By: System
Sample Type: Standard Sample Set Name: 260219, 2602_0803
Vial: 99, 100, 98 Acq. Method Set: scopoletin
Injection #: 1 Processing Method: scopoletin_ed
Injection Volume: 2.00, 20.00 ul Channel Name: 2475ChA ex360/em450
Run Time: 40.0 Minutes Proc. Chnl. Descr.: 2475ChA ex360/em450

Date Acquired: 26/02/2019 13:52:22 WIT, 26/02/2019 14:33:07 WIT, 26/02/2019 15:13:51 WIT, 08/03/2019
Date Processed: 12/03/2019 12:50:32 WIT, 12/03/2019 12:52:02 WIT, 12/03/2019 12:52:11 WIT, 12/03/2019

Scopoletin - 31.419

Scopoletin - 31.417
10000.0 10000.0
EU

EU
5000.0 5000.0

0.0 0.0

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
std scopoletin 1 std scopoletin 2
Scopoletin - 31.417

Scopoletin - 29.581
10000.0 10000.0
EU

EU

5000.0 5000.0

0.0 0.0

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
std scopoletin 3 std scopoletin 4
Scopoletin - 29.606

Scopoletin - 29.636

10000.0 10000.0
EU

EU

5000.0 5000.0

0.0 0.0

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
std scopoletin 5 std scopoletin 6

Reported by User: System Project Name: Scopoletin - FLD

Report Method: Multi Sample Summary Date Printed:
Report Method ID: 1270
1270 12/03/2019
Page: 1 of 2 12:53:54 Asia/Jakarta
 Name SampleName SampleWeight Dilution RT Area Amount Units
1 Scopoletin std scopoletin 1 1.00000 10.000 31.419 37902628 0.002 ppm
2 Scopoletin std scopoletin 2 1.00000 10.000 31.417 250109 0.001 ppm
3 Scopoletin std scopoletin 3 1.00000 10.000 31.417 287136939 0.005 ppm
4 Scopoletin std scopoletin 4 1.00000 1.000 29.581 487429860 0.100 ppm
5 Scopoletin std scopoletin 5 1.00000 1.000 29.606 1727528777 0.250 ppm
6 Scopoletin std scopoletin 6 1.00000 1.000 29.636 2730442297 0.500 ppm

Reported by User: System Project Name: Scopoletin - FLD

Report Method: Multi Sample Summary Date Printed:
Report Method ID: 1270
1270 12/03/2019
Page: 2 of 2 12:53:54 Asia/Jakarta
 Multi Sample Summary
SAMPLE INFORMATION
Sample Name: MGM 2, MGM 1, MGM 3 Acquired By: System
Sample Type: Unknown Sample Set Name: 2602_0803
Vial: 113, 115, 114 Acq. Method Set: scopoletin
Injection #: 1 Processing Method: scopoletin_ed
Injection Volume: 20.00 ul Channel Name: 2475ChA ex360/em450
Run Time: 40.0 Minutes Proc. Chnl. Descr.: 2475ChA ex360/em450

Date Acquired: 09/03/2019 01:33:19 WIT, 09/03/2019 02:14:04 WIT, 09/03/2019 02:54:49 WIT
Date Processed: 12/03/2019 12:57:35 WIT, 12/03/2019 12:57:39 WIT, 12/03/2019 12:57:45 WIT

1000.00 1000.00

Scopoletin - 31.417

Scopoletin - 31.407
800.00 800.00

600.00 600.00
EU

EU
400.00 400.00

200.00 200.00

0.00 0.00

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
MGM 1 MGM 2
1000.00
Scopoletin - 31.410

800.00

600.00
EU

400.00

200.00

0.00

10.00 20.00 30.00

Minutes
MGM 3

Name SampleName SampleWeight Dilution RT Area Amount Units

1 Scopoletin MGM 1 1.00000 1.000 31.417 161981668 0.031 ppm
2 Scopoletin MGM 2 1.00000 1.000 31.407 158737896 0.031 ppm
3 Scopoletin MGM 3 1.00000 1.000 31.410 159650546 0.031 ppm

Reported by User: System Project Name: Scopoletin - FLD

Report Method: Multi Sample Summary Date Printed:
Report Method ID: 1497
1497 12/03/2019
Page: 1 of 1 12:57:56 Asia/Jakarta
 Multi Sample Summary
SAMPLE INFORMATION
Sample Name: OGM 3, OGM 1, OGM 2 Acquired By: System
Sample Type: Unknown Sample Set Name: 260219
Vial: 109, 108, 107 Acq. Method Set: scopoletin
Injection #: 1 Processing Method: scopoletin_ed
Injection Volume: 20.00 ul Channel Name: 2475ChA ex360/em450
Run Time: 37.0 Minutes Proc. Chnl. Descr.: 2475ChA ex360/em450

Date Acquired: 26/02/2019 21:34:50 WIT, 26/02/2019 22:12:36 WIT, 26/02/2019 22:50:19 WIT
Date Processed: 12/03/2019 12:54:22 WIT

1000.00 1000.00

Scopoletin - 30.166

Scopoletin - 30.174
800.00 800.00

600.00 600.00
EU

EU
400.00 400.00

200.00 200.00

0.00 0.00

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
OGM 1 OGM 2
1000.00
Scopoletin - 30.186

800.00

600.00
EU

400.00

200.00

0.00

10.00 20.00 30.00

Minutes
OGM 3

Name SampleName SampleWeight Dilution RT Area Amount Units

1 Scopoletin OGM 1 1.00000 1.000 30.166 53679571 0.012 ppm
2 Scopoletin OGM 2 1.00000 1.000 30.174 60800455 0.013 ppm
3 Scopoletin OGM 3 1.00000 1.000 30.186 55588329 0.012 ppm

Reported by User: System Project Name: Scopoletin - FLD

Report Method: Multi Sample Summary Date Printed:
Report Method ID: 1497
1497 12/03/2019
Page: 1 of 1 12:55:42 Asia/Jakarta
 Multi Sample Summary
SAMPLE INFORMATION
Sample Name: PDG 1, PDG 2, PDG 3 Acquired By: System
Sample Type: Unknown Sample Set Name: 260219
Vial: 105, 106, 104 Acq. Method Set: scopoletin
Injection #: 1 Processing Method: scopoletin_ed
Injection Volume: 20.00 ul Channel Name: 2475ChA ex360/em450
Run Time: 37.0 Minutes Proc. Chnl. Descr.: 2475ChA ex360/em450

Date Acquired: 26/02/2019 19:41:35 WIT, 26/02/2019 20:19:18 WIT, 26/02/2019 20:57:07 WIT
Date Processed: 12/03/2019 12:54:18 WIT

1000.00 1000.00

Scopoletin - 30.169

Scopoletin - 30.156
800.00 800.00

600.00 600.00
EU

EU
400.00 400.00

200.00 200.00

0.00 0.00

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
PDG 1 PDG 2
1000.00
Scopoletin - 30.161

800.00

600.00
EU

400.00

200.00

0.00

10.00 20.00 30.00

Minutes
PDG 3

Name SampleName SampleWeight Dilution RT Area Amount Units

1 Scopoletin PDG 1 1.00000 1.000 30.169 141691482 0.028 ppm
2 Scopoletin PDG 2 1.00000 1.000 30.156 88163832 0.018 ppm
3 Scopoletin PDG 3 1.00000 1.000 30.161 103522801 0.021 ppm

Reported by User: System Project Name: Scopoletin - FLD

Report Method: Multi Sample Summary Date Printed:
Report Method ID: 1497
1497 12/03/2019
Page: 1 of 1 12:58:23 Asia/Jakarta
 Multi Sample Summary
SAMPLE INFORMATION
Sample Name: UDG 2, UDG 1, UDG 3 Acquired By: System
Sample Type: Unknown Sample Set Name: 2602_0803
Vial: 110, 112, 111 Acq. Method Set: scopoletin
Injection #: 1 Processing Method: scopoletin_ed
Injection Volume: 20.00 ul Channel Name: 2475ChA ex360/em450
Run Time: 40.0 Minutes Proc. Chnl. Descr.: 2475ChA ex360/em450

Date Acquired: 08/03/2019 23:31:04 WIT, 09/03/2019 00:11:49 WIT, 09/03/2019 00:52:35 WIT
Date Processed: 12/03/2019 12:56:23 WIT, 12/03/2019 12:56:30 WIT, 12/03/2019 12:56:41 WIT

1000.00 1000.00

Scopoletin - 31.427

Scopoletin - 31.405
800.00 800.00

600.00 600.00
EU

EU
400.00 400.00

200.00 200.00

0.00 0.00

10.00 20.00 30.00 10.00 20.00 30.00

Minutes Minutes
UDG 1 UDG 2
1000.00
Scopoletin - 31.401

800.00

600.00
EU

400.00

200.00

0.00

10.00 20.00 30.00

Minutes
UDG 3

Name SampleName SampleWeight Dilution RT Area Amount Units

1 Scopoletin UDG 1 1.00000 1.000 31.427 198368479 0.038 ppm
2 Scopoletin UDG 2 1.00000 1.000 31.405 118546164 0.024 ppm
3 Scopoletin UDG 3 1.00000 1.000 31.401 117450675 0.023 ppm

Reported by User: System Project Name: Scopoletin - FLD

Report Method: Multi Sample Summary Date Printed:
Report Method ID: 1497
1497 12/03/2019
Page: 1 of 1 12:56:58 Asia/Jakarta