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    Iván Andrés Benavides pajoy
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    This document summarizes the results of an efficiency test to activate a chromatographic column. Four standard compounds - phenol, 4-chloronitrobenzene, toluene, and naphthalene - were injected into the column in multiple runs. The retention times, peak areas, asymmetry factors, plate counts, signal-to-noise ratios, and resolution values were measured for each peak in each run. The results showed good separation and efficiency of the column.

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    PRUEBA DE EFICIENCIA-

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    This document summarizes the results of an efficiency test to activate a chromatographic column. Four standard compounds - phenol, 4-chloronitrobenzene, toluene, and naphthalene - were injected into the column in multiple runs. The retention times, peak areas, asymmetry factors, plate counts, signal-to-noise ratios, and resolution values were measured for each peak in each run. The results showed good separation and efficiency of the column.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    5 views11 pages

    Prueba de Eficiencia

    Uploaded by

    Iván Andrés Benavides pajoy
    This document summarizes the results of an efficiency test to activate a chromatographic column. Four standard compounds - phenol, 4-chloronitrobenzene, toluene, and naphthalene - were injected into the column in multiple runs. The retention times, peak areas, asymmetry factors, plate counts, signal-to-noise ratios, and resolution values were measured for each peak in each run. The results showed good separation and efficiency of the column.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 11

    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 1 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION COMBINADA Run Time (min): 10,00
    Vial Number: BA2 Injection Volume: 5,00
    Injection Type: Check Standard Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 08:55 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,790 3,377 0,99 20119 835,1 0,00
    2 4-CL-NITROBENCENO 4,107 0,950 0,96 17393 201,4 2,74
    3 TOLUENO 4,333 4,121 1,02 21141 903,5 4,81
    4 NAFTALENO 5,013 2,849 0,96 19694 524,8 9,80
    Total: 11,298 3,932 78347,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 2 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION COMBINADA Run Time (min): 10,00
    Vial Number: BA2 Injection Volume: 5,00
    Injection Type: Check Standard Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 09:07 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,793 3,389 0,96 20076 1033,4 0,00
    2 4-CL-NITROBENCENO 4,107 0,957 0,98 17487 251,0 2,71
    3 TOLUENO 4,337 4,120 0,99 21208 1116,4 4,80
    4 NAFTALENO 5,013 2,861 0,97 19697 651,6 9,76
    Total: 11,327 3,900 78468,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 3 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION COMBINADA Run Time (min): 10,00
    Vial Number: BA2 Injection Volume: 5,00
    Injection Type: Check Standard Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 09:19 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,790 3,397 1,00 20107 916,9 0,00
    2 4-CL-NITROBENCENO 4,107 0,959 0,96 17405 222,2 2,74
    3 TOLUENO 4,333 4,101 1,02 21073 981,1 4,80
    4 NAFTALENO 5,013 2,869 0,96 19669 578,2 9,79
    Total: 11,326 3,940 78254,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 4 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION COMBINADA Run Time (min): 10,00
    Vial Number: BA2 Injection Volume: 5,00
    Injection Type: Check Standard Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 09:30 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,793 3,397 0,96 20131 930,7 0,00
    2 4-CL-NITROBENCENO 4,107 0,959 0,98 17477 225,8 2,71
    3 TOLUENO 4,337 4,091 0,99 21179 993,9 4,81
    4 NAFTALENO 5,017 2,878 0,94 19801 587,7 9,80
    Total: 11,325 3,882 78588,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 5 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION COMBINADA Run Time (min): 10,00
    Vial Number: BA2 Injection Volume: 5,00
    Injection Type: Check Standard Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 09:42 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,793 3,405 0,97 20158 1068,4 0,00
    2 4-CL-NITROBENCENO 4,107 0,960 0,98 17423 259,1 2,71
    3 TOLUENO 4,337 4,076 1,00 21123 1132,9 4,80
    4 NAFTALENO 5,017 2,885 0,95 19783 674,3 9,80
    Total: 11,325 3,906 78487,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 6 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION TOLUENO Run Time (min): 10,00
    Vial Number: BA3 Injection Volume: 5,00
    Injection Type: Unknown Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 09:54 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    n.a. FENOL n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. 4-CL-NITROBENCENO n.a. n.a. n.a. n.a. n.a. n.a.
    1 TOLUENO 4,340 41,525 0,94 21034 6614,7 n.a.
    n.a. NAFTALENO n.a. n.a. n.a. n.a. n.a. n.a.
    Total: 41,525 0,940 21034,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 7 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION TOLUENO Run Time (min): 10,00
    Vial Number: BA3 Injection Volume: 5,00
    Injection Type: Unknown Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 10:05 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    n.a. FENOL n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. 4-CL-NITROBENCENO n.a. n.a. n.a. n.a. n.a. n.a.
    1 TOLUENO 4,343 41,356 0,92 21080 6969,4 n.a.
    n.a. NAFTALENO n.a. n.a. n.a. n.a. n.a. n.a.
    Total: 41,356 0,921 21080,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 8 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION NAFTALENO Run Time (min): 10,00
    Vial Number: BA4 Injection Volume: 5,00
    Injection Type: Unknown Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 10:17 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    n.a. FENOL n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. 4-CL-NITROBENCENO n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. TOLUENO n.a. n.a. n.a. n.a. n.a. n.a.
    1 NAFTALENO 5,017 28,520 0,96 19789 625,6 n.a.
    Total: 28,520 0,961 19789,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 9 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION NAFTALENO Run Time (min): 10,00
    Vial Number: BA4 Injection Volume: 5,00
    Injection Type: Unknown Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 10:29 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    n.a. FENOL n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. 4-CL-NITROBENCENO n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. TOLUENO n.a. n.a. n.a. n.a. n.a. n.a.
    1 NAFTALENO 5,017 28,469 0,97 19772 622,9 n.a.
    Total: 28,469 0,970 19772,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 10 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION FENOL Run Time (min): 10,00
    Vial Number: BA5 Injection Volume: 5,00
    Injection Type: Unknown Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 10:41 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,793 13,315 0,99 20160 255,4 0,00
    n.a. 4-CL-NITROBENCENO n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. TOLUENO n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. NAFTALENO n.a. n.a. n.a. n.a. n.a. n.a.
    Total: 13,315 0,987 20160,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925
    Instrument:HPLC_9 Sequence:Inicializacion Columna HiQ sil CN 110822 Page 11 of 11

    PRUEBA DE EFICIENCIA - ACTIVACION COLUMNA CROMATOGRAFICA


    COLUMNA HiQ Sil CN - LOTE 110822 / SERIE 00H00069
    AREA DE VALIDACIONES / GONHER FARMACEUTICA PLANTA II
    Injection Details
    Injection Name: SOLUCION FENOL Run Time (min): 10,00
    Vial Number: BA5 Injection Volume: 5,00
    Injection Type: Unknown Channel: UV_VIS_1
    Calibration Level: Wavelength: 254
    Instrument Method: Inicializacion columna CN Bandwidth: 1
    Processing Method: Basic Quantitative Dilution Factor: 1,0000
    Injection Date/Time: 25/sept./20 10:52 Sample Weight: 1,0000

    Chromatogram

    Integration Results
    No. Peak Name Retention Time Area Asymmetry (EP) Plates (USP) S/N Resolution (USP)
    min mAU*min
    1 FENOL 3,793 13,354 0,98 20166 258,4 0,00
    n.a. 4-CL-NITROBENCENO n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. TOLUENO n.a. n.a. n.a. n.a. n.a. n.a.
    n.a. NAFTALENO n.a. n.a. n.a. n.a. n.a. n.a.
    Total: 13,354 0,982 20166,00

    Chromeleon (c) Dionex


    Default/Integration Version 7.2.10.23925

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