D-7000 HPLC System Manager Report

Analyzed: 21/09/20 12:01 Reported: 22/09/20 07:29

Processed: 21/09/20 12:10
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 1
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 1 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,40

ACIDO ASCORBICO, 4,25

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1554337

1554337

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,31 7340 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 1 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 12:11 Reported: 22/09/20 07:29
Processed: 21/09/20 12:19
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 1
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 2 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,22

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,22 ACIDO ASCORBICO 1534136

1534136

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,22 ACIDO ASCORBICO 3,22 1,33 7300 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 2 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 12:21 Reported: 22/09/20 07:29
Processed: 21/09/20 12:29
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 1
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 3 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,34

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,34 ACIDO ASCORBICO 1576351

1576351

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,34 ACIDO ASCORBICO 3,34 1,32 7311 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 3 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 12:30 Reported: 22/09/20 07:29
Processed: 21/09/20 12:39
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 1
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 4 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,40

ACIDO ASCORBICO, 4,33

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,33 ACIDO ASCORBICO 1574415

1574415

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,33 ACIDO ASCORBICO 3,33 1,34 7417 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 4 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 12:40 Reported: 22/09/20 07:29
Processed: 21/09/20 12:49
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 1
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 5 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,32

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,32 ACIDO ASCORBICO 1574824

1574824

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,32 ACIDO ASCORBICO 3,32 1,32 7317 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 5 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 12:50 Reported: 22/09/20 07:29
Processed: 21/09/20 12:58
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 1
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 6 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,40

ACIDO ASCORBICO, 4,32

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,32 ACIDO ASCORBICO 1574523

1574523

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,32 ACIDO ASCORBICO 3,32 1,32 7383 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 6 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:00 Reported: 22/09/20 07:29
Processed: 21/09/20 13:08
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 2
Sample Name: VAL 1 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,20

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,20 ACIDO ASCORBICO 1396423

1396423

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,20 ACIDO ASCORBICO 3,20 1,30 7351 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 7 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:09 Reported: 22/09/20 07:29
Processed: 21/09/20 13:18
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 2
Sample Name: VAL 1 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1412052

1412052

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,32 7314 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 8 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:19 Reported: 22/09/20 07:29
Processed: 21/09/20 13:27
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 2
Sample Name: VAL 1 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,24

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,24 ACIDO ASCORBICO 1439723

1439723

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,24 ACIDO ASCORBICO 3,24 1,29 7239 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 9 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:29 Reported: 22/09/20 07:29
Processed: 21/09/20 13:37
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 2
Sample Name: VAL 1 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,24

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,24 ACIDO ASCORBICO 1420435

1420435

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,24 ACIDO ASCORBICO 3,24 1,32 7355 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 10 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:39 Reported: 22/09/20 07:29
Processed: 21/09/20 13:47
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 3
Sample Name: VAL 2 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1420367

1420367

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,30 7325 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 11 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:48 Reported: 22/09/20 07:29
Processed: 21/09/20 13:57
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 3
Sample Name: VAL 2 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,24

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,24 ACIDO ASCORBICO 1411121

1411121

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,24 ACIDO ASCORBICO 3,24 1,31 7302 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 12 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 13:58 Reported: 22/09/20 07:29
Processed: 21/09/20 14:06
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 3
Sample Name: VAL 2 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,22

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,22 ACIDO ASCORBICO 1414322

1414322

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,22 ACIDO ASCORBICO 3,22 1,32 7460 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 13 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 14:08 Reported: 22/09/20 07:29
Processed: 21/09/20 14:16
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 3
Sample Name: VAL 2 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,30

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,30 ACIDO ASCORBICO 1426464

1426464

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,30 ACIDO ASCORBICO 3,30 1,32 7315 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 14 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 14:18 Reported: 22/09/20 07:29
Processed: 21/09/20 14:26
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 4
Sample Name: VAL 3 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,27

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,27 ACIDO ASCORBICO 1425527

1425527

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,27 ACIDO ASCORBICO 3,27 1,28 7264 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 15 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 14:27 Reported: 22/09/20 07:29
Processed: 21/09/20 14:36
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 4
Sample Name: VAL 3 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,27

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,27 ACIDO ASCORBICO 1417811

1417811

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,27 ACIDO ASCORBICO 3,27 1,31 7290 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 16 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 14:37 Reported: 22/09/20 07:29
Processed: 21/09/20 14:45
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 4
Sample Name: VAL 3 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,27

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,27 ACIDO ASCORBICO 1431555

1431555

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,27 ACIDO ASCORBICO 3,27 1,31 7290 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 17 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 14:47 Reported: 22/09/20 07:29
Processed: 21/09/20 14:55
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 4
Sample Name: VAL 3 LOTE:5570919 MES 12 Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1418778

1418778

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7293 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 18 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 14:57 Reported: 22/09/20 07:29
Processed: 21/09/20 15:05
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 5
Sample Name: VAL 1 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,27

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,27 ACIDO ASCORBICO 1422128

1422128

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,27 ACIDO ASCORBICO 3,27 1,30 7304 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 19 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 15:06 Reported: 22/09/20 07:29
Processed: 21/09/20 15:15
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 5
Sample Name: VAL 1 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1431704

1431704

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,32 7256 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 20 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 15:16 Reported: 22/09/20 07:29
Processed: 21/09/20 15:24
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 5
Sample Name: VAL 1 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1422396

1422396

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,29 7282 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 21 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 15:26 Reported: 22/09/20 07:29
Processed: 21/09/20 15:34
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 5
Sample Name: VAL 1 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,27

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,27 ACIDO ASCORBICO 1445372

1445372

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,27 ACIDO ASCORBICO 3,27 1,32 7356 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 22 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 15:36 Reported: 22/09/20 07:29
Processed: 21/09/20 15:44
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 6
Sample Name: VAL 2 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1438716

1438716

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7371 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 23 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 15:45 Reported: 22/09/20 07:29
Processed: 21/09/20 15:54
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 6
Sample Name: VAL 2 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1419588

1419588

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,29 7319 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 24 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 15:55 Reported: 22/09/20 07:29
Processed: 21/09/20 16:03
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 6
Sample Name: VAL 2 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1428443

1428443

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,29 7345 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 25 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 16:05 Reported: 22/09/20 07:29
Processed: 21/09/20 16:13
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 6
Sample Name: VAL 2 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1425754

1425754

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,29 7285 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 26 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 16:14 Reported: 22/09/20 07:29
Processed: 21/09/20 16:23
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 7
Sample Name: VAL 3 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1422635

1422635

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,32 7293 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 27 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 16:24 Reported: 22/09/20 07:29
Processed: 21/09/20 16:33
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 7
Sample Name: VAL 3 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1424877

1424877

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7296 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 28 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 16:34 Reported: 22/09/20 07:29
Processed: 21/09/20 16:42
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 7
Sample Name: VAL 3 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1423566

1423566

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,29 7319 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 29 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 16:44 Reported: 22/09/20 07:29
Processed: 21/09/20 16:52
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 7
Sample Name: VAL 3 L:5570919 MES 12 atisan Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1420623

1420623

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,30 7348 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 30 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 16:53 Reported: 22/09/20 07:29
Processed: 21/09/20 17:02
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 8
Sample Name: VAL 1 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1372370

1372370

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,32 7363 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 31 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 17:03 Reported: 22/09/20 07:29
Processed: 21/09/20 17:11
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 8
Sample Name: VAL 1 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1362013

1362013

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,30 7389 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 32 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 17:13 Reported: 22/09/20 07:29
Processed: 21/09/20 17:21
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 8
Sample Name: VAL 1 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1371107

1371107

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7386 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 33 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 17:22 Reported: 22/09/20 07:29
Processed: 21/09/20 17:31
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 8
Sample Name: VAL 1 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1381558

1381558

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,33 7389 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 34 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 17:32 Reported: 22/09/20 07:29
Processed: 21/09/20 17:41
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 9
Sample Name: VAL 2 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1362142

1362142

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7439 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 35 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 17:42 Reported: 22/09/20 07:29
Processed: 21/09/20 17:50
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 9
Sample Name: VAL 2 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1376008

1376008

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,31 7363 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 36 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 17:52 Reported: 22/09/20 07:29
Processed: 21/09/20 18:00
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 9
Sample Name: VAL 2 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1360049

1360049

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7424 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 37 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 18:01 Reported: 22/09/20 07:29
Processed: 21/09/20 18:10
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 9
Sample Name: VAL 2 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1365467

1365467

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,30 7439 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 38 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 18:11 Reported: 22/09/20 07:29
Processed: 21/09/20 18:19
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 10
Sample Name: VAL 3 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1368440

1368440

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,32 7363 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 39 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 18:21 Reported: 22/09/20 07:29
Processed: 21/09/20 18:29
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 10
Sample Name: VAL 3 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1367875

1367875

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,31 7439 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 40 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 18:31 Reported: 22/09/20 07:29
Processed: 21/09/20 18:39
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 10
Sample Name: VAL 3 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,26

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,26 ACIDO ASCORBICO 1367055

1367055

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,26 ACIDO ASCORBICO 3,26 1,30 7386 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 41 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 18:40 Reported: 22/09/20 07:29
Processed: 21/09/20 18:49
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 10
Sample Name: VAL 3 L:5570919 MES 12 pilopro Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,23

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,23 ACIDO ASCORBICO 1359414

1359414

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,23 ACIDO ASCORBICO 3,23 1,32 7429 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 42 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 18:50 Reported: 22/09/20 07:29
Processed: 21/09/20 18:58
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 11
Sample Name: VAL 1 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,23

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,23 ACIDO ASCORBICO 1352109

1352109

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,23 ACIDO ASCORBICO 3,23 1,30 7323 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 43 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:00 Reported: 22/09/20 07:29
Processed: 21/09/20 19:08
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 11
Sample Name: VAL 1 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,23

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,23 ACIDO ASCORBICO 1352204

1352204

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,23 ACIDO ASCORBICO 3,23 1,32 7441 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 44 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:10 Reported: 22/09/20 07:29
Processed: 21/09/20 19:18
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 11
Sample Name: VAL 1 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,23

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,23 ACIDO ASCORBICO 1352147

1352147

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,23 ACIDO ASCORBICO 3,23 1,33 7361 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 45 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:19 Reported: 22/09/20 07:29
Processed: 21/09/20 19:28
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 11
Sample Name: VAL 1 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,23

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,23 ACIDO ASCORBICO 1348583

1348583

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,23 ACIDO ASCORBICO 3,23 1,33 7441 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 46 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:29 Reported: 22/09/20 07:29
Processed: 21/09/20 19:37
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 12
Sample Name: VAL 2 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,23

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,23 ACIDO ASCORBICO 1351873

1351873

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,23 ACIDO ASCORBICO 3,23 1,30 7271 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 47 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:39 Reported: 22/09/20 07:29
Processed: 21/09/20 19:47
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 12
Sample Name: VAL 2 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,24

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,24 ACIDO ASCORBICO 1348210

1348210

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,24 ACIDO ASCORBICO 3,24 1,33 7434 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 48 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:49 Reported: 22/09/20 07:29
Processed: 21/09/20 19:57
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 12
Sample Name: VAL 2 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,24

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,24 ACIDO ASCORBICO 1353518

1353518

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,24 ACIDO ASCORBICO 3,24 1,33 7384 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 49 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 19:58 Reported: 22/09/20 07:29
Processed: 21/09/20 20:07
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 12
Sample Name: VAL 2 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,24

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,24 ACIDO ASCORBICO 1352112

1352112

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,24 ACIDO ASCORBICO 3,24 1,33 7381 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 50 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 20:08 Reported: 22/09/20 07:29
Processed: 21/09/20 20:16
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 13
Sample Name: VAL 3 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 1 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,25

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,25 ACIDO ASCORBICO 1372792

1372792

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,25 ACIDO ASCORBICO 3,25 1,30 7363 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 51 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 20:18 Reported: 22/09/20 07:29
Processed: 21/09/20 20:26
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 13
Sample Name: VAL 3 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 2 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,27

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,27 ACIDO ASCORBICO 1364495

1364495

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,27 ACIDO ASCORBICO 3,27 1,31 7406 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 52 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 20:28 Reported: 22/09/20 07:29
Processed: 21/09/20 20:36
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 13
Sample Name: VAL 3 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 3 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,30

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,30 ACIDO ASCORBICO 1374100

1374100

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,30 ACIDO ASCORBICO 3,30 1,34 7393 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 53 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 20:37 Reported: 22/09/20 07:29
Processed: 21/09/20 20:46
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 13
Sample Name: VAL 3 L:4160719 MES 12 pilopro Vial Type: UNK
Injection from this vial: 4 of 4 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm

0,35

ACIDO ASCORBICO, 4,31

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,31 ACIDO ASCORBICO 1372910

1372910

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA Sample Amount: 1
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,31 ACIDO ASCORBICO 3,31 1,31 7492 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 54 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 20:47 Reported: 22/09/20 07:29
Processed: 21/09/20 20:55
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 14
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 1 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,31

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,31 ACIDO ASCORBICO 1528744

1528744

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,31 ACIDO ASCORBICO 3,31 1,34 7492 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 55 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 20:57 Reported: 22/09/20 07:29
Processed: 21/09/20 21:05
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 14
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 2 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,31

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,31 ACIDO ASCORBICO 1530179

1530179

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,31 ACIDO ASCORBICO 3,31 1,33 7480 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 56 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 21:06 Reported: 22/09/20 07:29
Processed: 21/09/20 21:15
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 14
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 3 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,30

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,30 ACIDO ASCORBICO 1528675

1528675

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,30 ACIDO ASCORBICO 3,30 1,32 7457 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 57 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 21:16 Reported: 22/09/20 07:29
Processed: 21/09/20 21:25
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 14
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 4 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,30

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,30 ACIDO ASCORBICO 1522071

1522071

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,30 ACIDO ASCORBICO 3,30 1,32 7514 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 58 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 21:26 Reported: 22/09/20 07:29
Processed: 21/09/20 21:34
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 14
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 5 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,22

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,22 ACIDO ASCORBICO 1491365

1491365

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,22 ACIDO ASCORBICO 3,22 1,33 7460 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 59 / 60
 D-7000 HPLC System Manager Report
Analyzed: 21/09/20 21:36 Reported: 22/09/20 07:29
Processed: 21/09/20 21:44
Data Path: C:\DATOS HPLC\2020\DATA\0829\
Processing Method: acido ascorbico
System(acquisition): CONTROL C Series:0829
Application: 2020 INI Vial Number: 14
Sample Name: STD ACIDO ASCORBICO L:Y06390 Vial Type: STD1
Injection from this vial: 6 of 6 Volume: 5,0 ul
Sample Description:
Analista:claudia ruiz longitud de onda:245nm
Chrom Type: Fixed WL Chromatogram, 245 nm
0,40

ACIDO ASCORBICO, 4,20

0,35

0,30
Absorbance (AU)

0,25

0,20

0,15

0,10

0,05

0,00

0 1 2 3 4 5 6 7 8
Retention Time (min)

RT Name Area

4,20 ACIDO ASCORBICO 1486342

1486342

Peak rejection level: 0

SST Report Algorithm Name: System Suitability Report (Half Width, EUP)
Acquisition Method: acido ascorbico
Column Type: Developed by:
Pump A Type: L-7100
Solvent A: H2O Solvent B: METANOL
Solvent C: ACN Solvent D: BUFFER
Chrom Type: Fixed WL Chromatogram, 245 nm
Peak Quantitation: AREA
Calculation Method: EXT-STD
Scale Factor 1: 1,000
RT Name k' Asym N Res Alpha S/N Noise
(min) (EUP) (EUP) (uV)

4,20 ACIDO ASCORBICO 3,20 1,31 7401 --- --- --- ---

Asymmetry warning outside the range of: 0,800 to 2,000

No. of theoretical plates warning at less than: 1000
Resolution warning at less than: 0,800
Signal to noise ratio warning at less than: 1

Page Indicator 60 / 60