UV DETECTOR LINEARITY REPORT

A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Acquired By: ryan

Processing Method: UV Detector Area PM, UV Detector Sample Kit 2 Lot Number: 1245
Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Sample Kit 2 Exp. Date: 12/22/2014 12:00:00 AM
Sample Set Id: 4678 Column Serial Number: W1412341AD
Sample Set Name: UV Detector Lin Sens SSM Result Set Id: 4722
Sample Set Start Date: 12/22/2014 10:32:44 PM EST

TE S T D E S C R I P TI O N
Detector linearity is performed to ensure that the detector has a linear response within its specified range.
The system makes several injections of a caffeine standard at different concentrations using the same
injection volume, and then Empower plots the Area and Height against the Amount. The coefficient of
determination (R^2) is used to measure the detector's linearity. The UV detector's sensitivity is determined
by dividing the height and area by the amount.

The qualification passes if R^2 > or = 0.990 for Height and R^2 > or = 0.990 for Area. The detector
sensitivity for Area must be < or = 4.5% and the Height %RSD < or = 4.5%.

UV Detector Area Linearity and Sensitiv ity Results

Area Linearity Area Linearity
Area Linearity Area Sensitivity Area Sensitivity Area Sensitivity
Curve R^2 Curve R^2
Curve R^2 % RSD Spec % RSD Pass/Fail
Spec Pass/Fail
1 R^2 > or = 0.990 0.9999 PASS %RSD < or = 4.5 1.06 PASS

UV Detector Height Linearity and Sensitiv ity Results

Height Linearity Height Linearity
Height Linearity Height Sensitivity Height Sensitivity
Curve R^2 Curve R^2 Height Sensitivity Spec
Curve R^2 % RSD Pass/Fail
Spec Pass/Fail
1 R^2 > or = 0.990 0.9991 PASS %RSD < or = 4.5 2.26 PASS

Printed 10:20:15 AM US/Eastern 12/30/2014 Page: 1 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 UV DETECTOR LINEARITY REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4678

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4722

Caffeine Detector Linearity Ov erlaid Chromatograms

1.60

1.40

1.20

1.00
AU

0.80

0.60

0.40
Caffeine - 0.746

0.20

0.00

0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40

Minutes
Sample Name: UV DetLin_1
Sample Name: UV DetLin_2
Sample Name: UV DetLin_3
Sample Name: UV DetLin_4
Sample Name: UV DetLin_5
Sample Name: UV DetLin_6
Sample Name: UV DetLin_7

Printed 10:20:14 AM US/Eastern 12/30/2014 Page: 2 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 UV DETECTOR LINEARITY REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4678

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4722

Area Calibration Plot

8x106

6x106

4x106
Area

2x106

0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16

Amount

Area Calibration Summary

Fit Type Processing Method Slope Y-Intercept R^2
1 Linear (1st Order) UV Detector Area PM 4.61e+007 4.13e+004 0.9999

Area Results
Vial Level SampleName Area Amount Area Sensitivity Peak Type Result Id
1 15 1 UV DetLin_1 475348 0.010 47534760.787 Found 4756
2 30 2 UV DetLin_2 1407692 0.030 46923053.058 Found 4757
3 45 3 UV DetLin_3 1906388 0.040 47659703.092 Found 4758
4 65 4 UV DetLin_4 2360598 0.050 47211955.422 Found 4759
5 75 5 UV DetLin_5 3741142 0.080 46764272.044 Found 4760
6 90 6 UV DetLin_6 4678396 0.100 46783964.360 Found 4761
7 105 7 UV DetLin_7 7394339 0.160 46214619.586 Found 4762

UV Detector Area Linearity

and Sensitiv ity Summary
Results
Area
Area
Linearity
Sensitivity % RSD
Curve R^2
1 0.9999 1.06

Printed 10:20:14 AM US/Eastern 12/30/2014 Page: 3 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00
 UV DETECTOR LINEARITY REPORT
A C Q U I S I TI O N I N F O R M A TI O N

System Name: 2695_2998_ISM_QDa72 Sample Set Id: 4678

Project Name: SQT_E_2695_2998_QDa_P_ISM_RC2 Result Set Id: 4722

Height Calibration Plot

1.5x106

1.0x106
Height

5.0x105

0.0

0.00 0.02 0.04 0.06 0.08 0.10 0.12 0.14 0.16

Amount

Height Calibration Summary

Fit Type Processing Method Slope Y-Intercept R^2
1 Linear (1st Order) UV Detector Height PM 9.93e+006 1.85e+004 0.9991

Height Results
Height
Vial Level SampleName Amount Height Sensitivity Peak Type Result Id
(µV)
1 15 1 UV DetLin_1 105788 0.010 10578811.609 Found 4765
2 30 2 UV DetLin_2 304979 0.030 10165962.353 Found 4766
3 45 3 UV DetLin_3 424153 0.040 10603819.121 Found 4767
4 65 4 UV DetLin_4 516634 0.050 10332670.841 Found 4768
5 75 5 UV DetLin_5 821715 0.080 10271436.788 Found 4769
6 90 6 UV DetLin_6 1034852 0.100 10348519.267 Found 4770
7 105 7 UV DetLin_7 1588776 0.160 9929848.581 Found 4771

UV Detector Height Linearity

and Sensitiv ity Summary
Results
Height Linearity Height
Curve R^2 Sensitivity % RSD
1 0.9991 2.26

Printed 10:20:14 AM US/Eastern 12/30/2014 Page: 4 of 4

Waters Project Name and Version: SQT_E_2695_2998_QDa_P_ISM Version 1.00