DILUTOR ACCURACY & PRECISION REPORT

The Dilutor Precision and Accuracy test verifies whether a diluted volume is precise and accurate within specified
criteria. Six (6) injections of Caffeine are made at five different dilution factors. The deviation of these six
chromatograms is expressed by % RSD. For Dilutor Accuracy the expected amount is compared to the calculated
amount and expressed in % Error.

The % RSD for the Caffeine Area should be < or = 1.0 % and the Average % Error should be < or = 2.0 %.

Dilutor Accuracy and Precision Results Table

Dilution Factor: 50

Average % Error Average AVE % Error Area % RSD Area Area % RSD
Specification % Error Pass Fail Specification % RSD Pass Fail
1 Average % Error <or= 2.0 1.5 Pass % RSD = 1.0 0.2 Pass

Dilution Factor: 25

Average % Error Average AVE % Error Area % RSD Area Area % RSD
Specification % Error Pass Fail Specification % RSD Pass Fail
1 Average % Error <or= 2.0 0.8 Pass % RSD = 1.0 0.07 Pass

Dilution Factor: 10

Average % Error Average AVE % Error Area % RSD Area Area % RSD
Specification % Error Pass Fail Specification % RSD Pass Fail
1 Average % Error <or= 2.0 0.8 Pass % RSD = 1.0 0.76 Pass

Dilution Factor: 5

Average % Error Average AVE % Error Area % RSD Area Area % RSD
Specification % Error Pass Fail Specification % RSD Pass Fail
1 Average % Error <or= 2.0 0.2 Pass % RSD = 1.0 0.03 Pass

Dilution Factor: 2

Average % Error Average AVE % Error Area % RSD Area Area % RSD
Specification % Error Pass Fail Specification % RSD Pass Fail
1 Average % Error <or= 2.0 0.7 Pass % RSD = 1.0 0.02 Pass

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 DILUTOR ACCURACY & PRECISION REPORT
AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 060 Dilutor Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Precision PM_Area Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Acc Prec Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11631 DB ID:
Result 847558692
Set Id 11644
Sample Set Name: Dilutor Accuracy Sample Set Start Date: 11/4/2010 6:31:25 PM

Printed 7:56:05 AM US/Eastern 11/17/2010 Page: 2 of 5

 DILUTOR ACCURACY & PRECISION REPORT
AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 060 Dilutor Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Precision PM_Area Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Acc Prec Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11631 DB ID:
Result 847558692
Set Id 11644
Sample Set Name: Dilutor Accuracy Sample Set Start Date: 11/4/2010 6:31:25 PM

Peak Results
SampleName: Dilutor Acc_8
SampleName Injection Area Amount %Error
1 Dilutor Acc_8 1 2026278 51.553 1.553
2 Dilutor Acc_8 2 2027787 51.592 1.592
3 Dilutor Acc_8 3 2024376 51.503 1.503
4 Dilutor Acc_8 4 2022634 51.458 1.458
5 Dilutor Acc_8 5 2022305 51.449 1.449
6 Dilutor Acc_8 6 2017711 51.330 1.330
Mean 2023515 51.481 1.48
% RSD 0.17

Peak Results
SampleName: Dilutor Acc_9
SampleName Injection Area Amount %Error
1 Dilutor Acc_9 1 3854313 99.133 0.867
2 Dilutor Acc_9 2 3852249 99.080 0.920
3 Dilutor Acc_9 3 3854449 99.137 0.863
4 Dilutor Acc_9 4 3856243 99.184 0.816
5 Dilutor Acc_9 5 3856038 99.178 0.822
6 Dilutor Acc_9 6 3860453 99.293 0.707
Mean 3855624 99.167 0.83
% RSD 0.07

Peak Results
SampleName: Dilutor Acc_10
SampleName Injection Area Amount %Error
1 Dilutor Acc_10 1 259096 5.556 0.694
2 Dilutor Acc_10 2 254953 5.448 0.802
3 Dilutor Acc_10 3 254767 5.444 0.806

Printed 7:56:05 AM US/Eastern 11/17/2010 Page: 3 of 5

 DILUTOR ACCURACY & PRECISION REPORT
AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 060 Dilutor Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Precision PM_Area Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Acc Prec Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11631 DB ID:
Result 847558692
Set Id 11644
Sample Set Name: Dilutor Accuracy Sample Set Start Date: 11/4/2010 6:31:25 PM

Peak Results
SampleName: Dilutor Acc_10
SampleName Injection Area Amount %Error
4 Dilutor Acc_10 4 254478 5.436 0.814
5 Dilutor Acc_10 5 253691 5.416 0.834
6 Dilutor Acc_10 6 254452 5.435 0.815
Mean 255239 5.456 0.79
% RSD 0.76

Peak Results
SampleName: Dilutor Acc_11
SampleName Injection Area Amount %Error
1 Dilutor Acc_11 1 998235 24.795 0.205
2 Dilutor Acc_11 2 998363 24.798 0.202
3 Dilutor Acc_11 3 999019 24.815 0.185
4 Dilutor Acc_11 4 998518 24.802 0.198
5 Dilutor Acc_11 5 999071 24.816 0.184
6 Dilutor Acc_11 6 998813 24.810 0.190
Mean 998670 24.806 0.19
% RSD 0.03

Peak Results
SampleName: Dilutor Acc_12
SampleName Injection Area Amount %Error
1 Dilutor Acc_12 1 1994556 50.727 0.727
2 Dilutor Acc_12 2 1994137 50.716 0.716
3 Dilutor Acc_12 3 1995071 50.741 0.741
4 Dilutor Acc_12 4 1994690 50.731 0.731
5 Dilutor Acc_12 5 1995036 50.740 0.740
6 Dilutor Acc_12 6 1993873 50.709 0.709
Mean 1994560 50.727 0.73
% RSD 0.02

Printed 7:56:05 AM US/Eastern 11/17/2010 Page: 4 of 5

 DILUTOR ACCURACY & PRECISION REPORT
AC Q U I SI TI O N I N F O R M ATI O N

System Name: PATROL with PDA Acquired By: Jennb

Project Name: SQT_PATrol_PDA_CM_rev D Column serial Number: 11581505610K
Sample Set Method: 060 Dilutor Accuracy Sample Kit 1 Expiration date: 10/22/2010 12:00:00 AM
Processing Method Dilutor Precision PM_Area Sample Kit 1 Lot Number: N070015
Report Method Name: Dilutor Acc Prec Report Build Version: Empower 2 Software Build 2154 SPs
User Name: System Installed:
Sample Set Feature Release 5
Id: 11631 DB ID:
Result 847558692
Set Id 11644
Sample Set Name: Dilutor Accuracy Sample Set Start Date: 11/4/2010 6:31:25 PM

Overlay Chromatograms
2.20

2.00

1.80
Caffeine
1.60

1.40

1.20
AU

1.00

0.80

0.60

0.40

0.20

0.00

0.60 0.70 0.80 0.90 1.00 1.10 1.20 1.30 1.40 1.50 1.60
Minutes

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