20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.

xad Page 1 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electrophoresis File Run Summary

Instrument Information:
Instrument Name: DE72905431 Firmware: C.01.069
Serial#: DE72905431 Type: G2938C
Assay Information:
Assay Origin Path: C:\Program Files\Agilent\2100 bioanalyzer\2100
expert\assays\dsDNA\High Sensitivity DNA.xsy

Assay Class: High Sensitivity DNA Assay

Version: 1.03
Assay Comments: Copyright © 2003-2010 Agilent Technologies

Chip Information:
Chip Lot #:
Reagent Kit Lot #:
Chip Comments:

E1-1 E1-2 E1-3

E3-1 E3-2 E3-3

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 2 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electrophoresis File Run Summary (Chip Summary)

Sample Name Sample Rest. Statu Observation Result Label Result Color
Comment Digest s
E1-1
E1-2
E1-3
E3-1
E3-2
E3-3
sample 7
sample 8
sample 9
sample 10
sample 11
Ladder

Chip Lot # Reagent Kit Lot #

Chip Comments :

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 3 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electrophoresis Assay Details

General Analysis Settings

Number of Available Sample and Ladder Wells (Max.) : 12
Minimum Visible Range [s] : 32
Maximum Visible Range [s] : 138
Start Analysis Time Range [s] : 33
End Analysis Time Range [s] : 137.5
Ladder Concentration [pg/µl] : 1950
Uses Standard Area for Ladder Fragments
Lower Marker Concentration [pg/µl] : 125
Upper Marker Concentration [pg/µl] : 75
Used Upper Marker for Quantitation
Standard Curve Fit is Point to Point
Show Data Aligned to Lower and Upper Marker

Integrator Settings
Integration Start Time [s] : 33.05
Integration End Time [s] : 137
Slope Threshold : 0.8
Height Threshold [FU] : 5
Area Threshold : 0.1
Width Threshold [s] : 0.6
Baseline Plateau [s] : 0.5

Filter Settings
Filter Width [s] : 0.5
Polynomial Order : 4

Ladder
Ladder Peak Size Area
1 35 160
2 50 210
3 100 208
4 150 221
5 200 242
6 300 270
7 400 305
8 500 306
9 600 336
10 700 321
11 1000 366
12 2000 413
13 3000 411
14 7000 400
15 10380 214

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 4 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary

Ladder

Overall Results for Ladder

Noise: 0.5

Peak table for Ladder

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 50 150.00 4,545.5 Ladder Peak
3 100 150.00 2,272.7 Ladder Peak
4 150 150.00 1,515.2 Ladder Peak
5 200 150.00 1,136.4 Ladder Peak
6 300 150.00 757.6 Ladder Peak
7 400 150.00 568.2 Ladder Peak
8 500 150.00 454.5 Ladder Peak
9 600 150.00 378.8 Ladder Peak
10 700 150.00 324.7 Ladder Peak
11 1,000 150.00 227.3 Ladder Peak
12 2,000 150.00 113.6 Ladder Peak
13 3,000 150.00 75.8 Ladder Peak
14 7,000 150.00 32.5 Ladder Peak
15 10,380 75.00 10.9 Upper Marker

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 5 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary Continued ...

E1-1

Overall Results for sample 1 : E1-1

Number of peaks found: 6 Corr. Area 1: 4,513.5
Noise: 0.5

Peak table for sample 1 : E1-1

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 321 821.80 3,880.7
3 339 249.19 1,112.3
4 354 166.26 712.6
5 384 134.95 532.1
6 395 174.81 670.2
7 417 1,827.94 6,634.4
8 10,380 75.00 10.9 Upper Marker

Region table for sample 1 : E1-1

From To [bp] Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor
200 1,000 4,513.5 99 436 28.5 3,857.10 14,749.1

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
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Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary Continued ...

E1-2

Overall Results for sample 2 : E1-2

Number of peaks found: 8 Corr. Area 1: 2,779.5
Noise: 0.5

Peak table for sample 2 : E1-2

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 320 542.56 2,569.9
3 339 211.74 947.5
4 347 144.26 629.9
5 360 152.11 640.3
6 385 135.43 532.8
7 393 109.04 420.5
8 415 777.43 2,836.1
9 864 4.75 8.3
10 10,380 75.00 10.9 Upper Marker

Region table for sample 2 : E1-2

From To [bp] Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor
200 1,000 2,779.5 99 407 26.2 2,138.28 8,636.8

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 7 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary Continued ...

E1-3

Overall Results for sample 3 : E1-3

Number of peaks found: 7 Corr. Area 1: 3,296.9
Noise: 0.4

Peak table for sample 3 : E1-3

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 320 640.38 3,036.7
3 349 756.83 3,289.0
4 397 115.50 440.8
5 412 154.21 567.8
6 433 293.18 1,026.3
7 469 778.96 2,516.9
8 1,077 2.90 4.1
9 10,380 75.00 10.9 Upper Marker

Region table for sample 3 : E1-3

From To [bp] Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor
200 1,000 3,296.9 99 425 27.3 2,818.52 10,980.2

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 8 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary Continued ...

E3-1

Overall Results for sample 4 : E3-1

Number of peaks found: 11 Corr. Area 1: 2,289.2
Noise: 0.4

Peak table for sample 4 : E3-1

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 319 294.14 1,396.5
3 351 290.96 1,256.3
4 362 108.96 455.5
5 373 136.87 556.6
6 394 73.45 282.8
7 415 98.78 361.0
8 432 306.03 1,074.3
9 492 292.53 900.6
10 597 85.96 218.0
11 695 64.94 141.5
12 10,380 75.00 10.9 Upper Marker
13 11,998 0.00 0.0

Region table for sample 4 : E3-1

From To [bp] Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor
200 1,000 2,289.2 99 439 28.2 1,810.62 6,864.3

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 9 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary Continued ...

E3-2

Overall Results for sample 5 : E3-2

Number of peaks found: 6 Corr. Area 1: 3,347.7
Noise: 0.4

Peak table for sample 5 : E3-2

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 319 587.18 2,789.3
3 350 389.55 1,686.5
4 364 329.66 1,373.1
5 391 176.10 683.0
6 414 164.08 600.6
7 429 1,001.22 3,533.5
8 10,380 75.00 10.9 Upper Marker

Region table for sample 5 : E3-2

From To [bp] Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor
200 1,000 3,347.7 99 422 26.9 2,609.94 10,210.2

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 10 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Electropherogram Summary Continued ...

E3-3

Overall Results for sample 6 : E3-3

Number of peaks found: 9 Corr. Area 1: 2,805.8
Noise: 0.4

Peak table for sample 6 : E3-3

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 319 630.71 2,999.6
3 348 347.84 1,512.4
4 364 279.02 1,162.1
5 386 92.79 364.2
6 412 108.77 400.4
7 426 309.03 1,098.7
8 474 541.22 1,730.8
9 727 49.32 102.7
10 10,380 75.00 10.9 Upper Marker
11 12,874 0.00 0.0

Region table for sample 6 : E3-3

From To [bp] Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor
200 1,000 2,805.8 99 420 28.5 2,424.97 9,621.6

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 11 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Gel Image

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
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Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Invalid Samples

Sample 7 has not been run, no results available.

Sample 8 has not been run, no results available.
Sample 9 has not been run, no results available.
Sample 10 has not been run, no results available.
Sample 11 has not been run, no results available.

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM
20220919_High Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Page 13 of 13

Assay Class: High Sensitivity DNA Assay Created: 9/19/2022 1:49:11 PM

Data Path: C:\...gh Sensitivity DNA Assay_DE72905431_2022-09-19_13-49-11.xad Modified: 9/19/2022 2:16:12 PM
Run Logbook

Description Number Source Category Sub Category Time Time Zone User Host
Run ended on Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
port 1 (Number 2:16:10 PM W. Europe
of wells Standard Time
acquired: 7)
Run started on Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
port 1 (File: 1:49:16 PM W. Europe
C:\Program Standard Time
Files\Agilent\21
00
bioanalyzer\210
0
expert\Data\202
2-09-19\202209
19_High
Sensitivity DNA
Assay_DE72905
431_2022-09-19
_13-49-11.xad)

Product Number Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER

: G2938C 1:49:16 PM W. Europe
Standard Time
Name : Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
1:49:16 PM W. Europe
Standard Time
Vendor : Agilent Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
Technologies 1:49:16 PM W. Europe
Standard Time
Serial# : Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
DE72905431 1:49:16 PM W. Europe
Standard Time
Firmware : Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
C.01.069 1:49:16 PM W. Europe
Standard Time
Cartridge : Instrument Run 9/19/2022 (GMT +02:00) Administrator BIOANALYZER
Electrode 1:49:16 PM W. Europe
Standard Time

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2015 Agilent Technologies, Inc. Printed: 9/19/2022 2:16:41 PM