23 11 21 ExtractProtocolJoan GoodLadder

2100 expert_Plant RNA Nano_DE34903737_2023-11-21_11-59-42.
xad Page 1 of 18
Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Data Path: C:\...00 expert_Plant RNA Nano_DE34903737_2023-11-21_11-59-42.xad Modified: 11/21/2023 12:23:39 PM
Electrophoresis File Run Summary
Instrument Information:
Instrument Name: DE34903737 Firmware: C.01.069
Serial#: DE34903737 Type: G2938C
Assay Information:
C:\Program Files\Agilent\2100 bioanalyzer\2100
expert\assays\RNA\Plant RNA Nano.xsy
Assay Origin Path:
Assay Class: Plant RNA Nano

Version: 1.3
Assay Comments: Total RNA Analysis ng sensitivity (Plant)
© Copyright 2003 - 2009 Agilent Technologies, Inc.

Chip Information:
Chip Lot #:
Reagent Kit Lot #:
Chip Comments:
Sample 1 Sample 2 Sample 3
RIN: 8.60 RIN: 8.30 RIN: 8.40
RIN: 8.10 RIN: 7.50 RIN: 7.20
RIN: 8.30 RIN N/A RIN N/A
RIN N/A RIN N/A RIN N/A
2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 11/21/2023 12:32:25 PM
2100 expert_Plant RNA Nano_DE34903737_2023-11-21_11-59-42.xad Page 2 of 18

Electrophoresis File Run Summary (Chip Summary)
Sample Name Sample Comment Status Result Label Result Color

Sample 1 RIN: 8.60
Sample 2 RIN: 8.30
Sample 3 RIN: 8.40
Sample 4 RIN: 8.10
Sample 5 RIN: 7.50
Sample 6 RIN: 7.20
Sample 7 RIN: 8.30
Sample 8 RIN N/A
Sample 9 RIN N/A
Sample 10 RIN N/A
Sample 11 RIN N/A
Sample 12 RIN N/A
Ladder All Other Samples
Chip Lot # Reagent Kit Lot #
Chip Comments :

Electrophoresis Assay Details
General Analysis Settings

Number of Available Sample and Ladder Wells (Max.) : 13
Minimum Visible Range [s] : 17
Maximum Visible Range [s] : 70
Start Analysis Time Range [s] : 19
End Analysis Time Range [s] : 69
Ladder Concentration [ng/µl] : 150
Lower Marker Concentration [ng/µl] : 0
Upper Marker Concentration [ng/µl] : 0
Used Lower Marker for Quantitation
Standard Curve Fit is Logarithmic
Show Data Aligned to Lower Marker
Integrator Settings
Integration Start Time [s] : 19
Integration End Time [s] : 69
Slope Threshold : 0.6
Height Threshold [FU] : 0.2
Area Threshold : 0.1
Width Threshold [s] : 0.2
Baseline Plateau [s] : 6
Filter Settings
Filter Width [s] : 0.5
Polynomial Order : 4
Ladder
Ladder Peak Size
1 25
2 200
3 500
4 1000
5 2000
6 4000

Electropherogram Summary
Ladder
Overall Results for Ladder

RNA Area: 330.9 Result Flagging Color:
RNA Concentration: 150 ng/µl Result Flagging Label: All Other Samples

Electropherogram Summary Continued ...
Sample 1
Overall Results for sample 1 : Sample 1

RNA Area: 136.1 RNA Integrity Number (RIN): 8.6 (B.02.08)
RNA Concentration: 62 ng/µl Result Flagging Color:
rRNA Ratio [25s / 18s]: 2.3 Result Flagging Label: RIN: 8.60
Fragment table for sample 1 : Sample 1

Name Start Size [nt] End Size [nt] Area % of total Area
18S 1,567 1,929 21.9 16.1
25S 2,444 3,179 50.9 37.4

Sample 2


18S 1,562 1,906 30.6 13.8
25S 2,392 3,189 70.6 31.8

Sample 3


18S 1,570 1,865 13.5 15.8
25S 2,366 3,279 33.1 38.7

Sample 4


18S 1,562 1,848 16.3 15.4
25S 2,546 3,240 32.0 30.2

Sample 5


18S 1,545 1,816 9.8 8.4
25S 2,366 3,214 22.0 18.8

Sample 6


18S 1,567 1,805 5.4 6.8
25S 2,508 3,102 13.2 16.6

Sample 7


18S 1,524 1,936 18.4 14.5
25S 2,470 3,129 45.7 36.0

Sample 8

RNA Area: 21.1 RNA Integrity Number (RIN): N/A (B.02.08)
rRNA Ratio [25s / 18s]: 2.4 Result Flagging Label: RIN N/A

18S 1,536 1,901 3.2 14.9
25S 2,509 3,134 7.6 36.1

Sample 9


Sample 10


Sample 11


Sample 12


Gel Image

Curves
Standard Curve

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2100 expert_Plant RNA Nano_DE34903737_2023-11-21_11-59-42.

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Assay Class: Plant RNA Nano

© Copyright 2003 - 2009 Agilent Technologies, Inc.

Sample 1 Sample 2 Sample 3

RIN: 8.60 RIN: 8.30 RIN: 8.40

Sample 4 Sample 5 Sample 6

RIN: 8.10 RIN: 7.50 RIN: 7.20

Sample 7 Sample 8 Sample 9

RIN: 8.30 RIN N/A RIN N/A

Sample 10 Sample 11 Sample 12

RIN N/A RIN N/A RIN N/A

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Sample Name Sample Comment Status Result Label Result Color

Chip Lot # Reagent Kit Lot #

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

General Analysis Settings

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for Ladder

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 1 : Sample 1

Fragment table for sample 1 : Sample 1

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 2 : Sample 2

Fragment table for sample 2 : Sample 2

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 3 : Sample 3

Fragment table for sample 3 : Sample 3

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 4 : Sample 4

Fragment table for sample 4 : Sample 4

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 5 : Sample 5

Fragment table for sample 5 : Sample 5

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 6 : Sample 6

Fragment table for sample 6 : Sample 6

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 7 : Sample 7

Fragment table for sample 7 : Sample 7

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 8 : Sample 8

Fragment table for sample 8 : Sample 8

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 9 : Sample 9

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 10 : Sample 10

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 11 : Sample 11

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Overall Results for sample 12 : Sample 12

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

Assay Class: Plant RNA Nano Created: 11/21/2023 11:59:42 AM

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