Operation

5.6 Prepare Reagents

Prepare Reagents

Prepare coagulation reagents needed for analysis, Owren’s Veronal

Buffer, and rinse solution. Refer to the package insert of each reagent for
more information.

Parame- Reagent Consumption per

ter Test
PT PT Reagent 100 µL
APTT APTT Reagent 50 µL
Calcium Chloride Solution (0.025 mol/L) 50 µL
Fbg Thrombin Reagent 50 µL
Owren’s Veronal Buffer 90 µL
TT Thrombin Clotting Time Reagent 100 µL
PCcl Protein C Deficient Plasma 45 µL
Protein C Activator 50 µL
PC APTT Reagent 50 µL
Calcium Chloride Solution 50 µL
BXT Batroxobin Reagent 100 µL
LA1 LA1 Screening Reagent 100 µL
LA2 LA2 Confirmation Reagent 100 µL
AT3 Berichrom® Antithrombin III (A)
Thrombin Reagent 125 µL
Substrate 33 µL
Owren’s Veronal Buffer 83 µL
BCPC Berichrom® Protein C
Protein C Activator 125 µL
Substrate 30 µL
® 2-Antiplasmin
APL* Berichrom
Antiplasmin Reagent 125 µL
Substrate 25 µL
Owren’s Veronal Buffer 112 µL
® Plasminogen
Plg* Berichrom
Revised June 2009 - 2.0_en

Streptokinase Reagent 125 µL

Substrate 25 µL
Owren’s Veronal Buffer 112 µL

5-8 Sysmex CA-500 series

 Operation

Parame- Reagent Consumption per

ter Test
Hep Berichrom® Heparin
AT3 Reagent 20 µL
FXa Reagent 125 µL
Heparin Substrate 40 µL
DDPl* D-Dimer PLUS
Accelerator 25 µL
Latex Reagent 150 µL
AdDD** Advanced D-Dimer
Accelerator 25 µL
Latex Reagent 150 µL
DDi INNOVANCE® D-Dimer
Diluent 12 µL (82 µL required
for redilution)
Supplement 16 µL
Buffer 80 µL
Reagent 44 µL
PFDP*** Latex Test BL-2 P-FDP
Reagent 66 µL
Latex 94 µL
P-FDP Diluents 112 µL
Rinse Distilled Water (Rinse Bottle) Max. 24 mL per test
Solution CA CLEAN I 10 µL more than the
required reagent
volume.
(*) Not available for use in the USA.
(**) Only available for use in the USA.
(***) Only available for use in Asia.

The amount of Owren’s Veronal Buffer per test includes the amount
used in dilution for each analysis parameter.
When analyzing the parameters that require two reagents, a rinse
operation is performed three times in total after dispensing samples and
reagents.
Thus the amount used per test is approximately 24 mL. However, the
Revised June 2009 - 2.0_en

number of rinse operations can be changed. (Refer to “10.8 Test

Protocol”.)
CA CLEAN I is used after the reagent is dispensed. The amount used for
one rinse is the amount of the reagent + 10 µL.

Sysmex CA-500 series 5-9