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Eur J Appl Physiol (2004) 93: 167–172

DOI 10.1007/s00421-004-1192-4

O R I GI N A L A R T IC L E

Fabio Esposito Æ Franco M. Impellizzeri


Vittoria Margonato Æ Rosalba Vanni Æ Giuliano Pizzini
Arsenio Veicsteinas

Validity of heart rate as an indicator of aerobic demand


during soccer activities in amateur soccer players

Accepted: 23 June 2004 / Published online: 22 July 2004


Ó Springer-Verlag 2004

Abstract In order to validate the use of heart rate (HR)


in describing and monitoring physiological demands Introduction
during soccer activities, the HR versus oxygen uptake
(V_ O2) relationship determined on the field during soc- Soccer is one of the most popular sports in the world
cer-specific exercises was compared to that found in the and several studies investigated the physiological de-
laboratory during treadmill exercise. Seven male ama- mands of soccer activities (Ekblom 1986; MacLaren
teur soccer players [mean (SE), age 25.3 (1.2) years, et al. 1988; Rohde and Espersen 1988; Van Gool et al.
body mass 72.9 (2.1) kg, stature 1.76 (0.03) m] per- 1988; Tumilty 1993; Bangsbo 1994a, b; Felci et al. 1995;
formed three trials on the field (two laps of a purpose- Reilly 1997). A widespread indirect method to evaluate
made circuit including a variety of soccer activities) at the metabolic demands during a soccer game or specific
different intensities (moderate, high and very high, training is to record heart rate (HR) during soccer
according to their rate of perceived exertion) and an activities, which is aided by the use of comfortable and
incremental test on a treadmill in the laboratory. HR accurate HR telemetric systems (Ali and Farrally 1991).
increased linearly with V_ O2 during both field and labo- However, a direct measurement of individual oxygen
ratory tests according to exercise intensity (P<0.01). uptake (V_ O2) in the laboratory should always be per-
The mean correlation coefficients of the HR–V_ O2 rela- formed in order to attempt to retrieve metabolic infor-
tionships obtained in the laboratory and on the field mation from HR measured on the field. Indeed, based
were 0.984 (0.012) and 0.991 (0.005) (P<0.001), re- on the linearity of the relationship between HR and V_ O2
spectively. The mean value of the HR–V_ O2 regression (Astrand and Rodahl 1977), the individual HR–V_ O2
equation slope and intercept obtained in laboratory regression line obtained in the laboratory could then be
[0.030 (0.002) and 79.6 (4.6), respectively] were not sig- used to determine the exercise intensity and the physi-
nificantly different compared to those found on the field ological demands for the specific HR measured on the
[0.032 (0.003) and 76.7 (9.7)]. The present study seems to field. However, this methodology can be applied only
confirm that HR measured during soccer exercises when the relationship determined in the laboratory has
effectively reflects the metabolic expenditure of this been verified to be similar to that found on the field
activity. Thus, with the aid of laboratory reference tests, during specific tasks at all exercise intensities.
the physiological demands of soccer activities can be Soccer is a very peculiar team sport, where continu-
correctly estimated from HR measured on the field in ous activities, such as walking and running, are often
amateur soccer players. interspersed with intermittent tasks, such as sprinting,
jumping, kicking and dribbling. These factors, together
Keywords Heart rate Æ Oxygen uptake Æ Soccer Æ with thermal and emotional stresses typical of compet-
Physiological evaluation itive soccer games, could introduce possible estimation
error sources and alter the linearity of the HR–V_ O2
relationship on the field, especially at low levels of effort.
F. Esposito (&) Æ F. M. Impellizzeri Æ V. Margonato Despite this indirect V_ O2 estimation strategy is com-
R. Vanni Æ G. Pizzini Æ A. Veicsteinas monly considered acceptable (Ekblom 1986; MacLaren
Insitute of Physical Exercise, Health and Sports, et al. 1988; Rohde and Espersen 1988; Van Gool et al.
University of Milan, Via Colombo 71, 1988; Bangsbo 1994a, Bangsbo b; Reilly 1997), Tumilty
20133 Milan, Italy
E-mail: fabio.esposito@unimi.it
(1993) suggested that HR may not correctly reflect V_ O2
Tel.: +39-02-50314649 during soccer activities. Moreover, Ogushi et al. (1993)
Fax: +39-02-50314649 measured V_ O2 in two college soccer players during a
168

simulated soccer game and found that exercise intensity light (600 g) portable unit used in this investigation,
expressed as V_ O2 was 25% lower than that estimated which also provided HR measurements, has been re-
using the HR–V_ O2 relationship determined in the lab- ported to be reliable and valid for field measurements
oratory. of soccer exercises (Kawakami et al. 1992), as well as
Some studies measured the exercise V_ O2 using por- those in the laboratory.
table gas analyzer systems in order to better understand
the physiological demands of various soccer-specific
exercises in amateur (Kawakami et al. 1992), profes- Field test
sional (Hoff et al. 2002) and youth (Felci et al. 1995)
soccer players. However, to our knowledge, the regres- In order to reproduce the typical soccer activities
sion line of the HR–V_ O2 relationship obtained on the (intermittent nature and movement patterns), a modified
field during soccer activities has never been compared to circuit from Ekblom (1998) was used (Fig. 1). The cir-
that obtained in the laboratory. cuit was proposed to test soccer players and reproduce
Thus, the aim of the present study was to compare the most important soccer activities, such as jumping,
the HR–V_ O2 relationship determined on the field during changes in direction, walking, jogging, running (with
soccer-specific tasks at different intensities to that found and without ball), forward, backward and lateral
in the laboratory during a standard treadmill test. movements, etc. To this original circuit, some other
Should the relationship be the same, the use of HR exercise modalities, such as passing and kicking, were
monitoring on the field would be a valid indicator of the added to include all the movement patterns and activi-
physiological demands of the soccer-specific exercises in ties commonly performed during soccer training or
amateur players. game.
Field tests were performed outdoors, where average
temperature ranged between 28 and 30°C, and relative
Methods humidity between 60 and 70%. After about 10 min of
warm up, consisting of jogging and running, a
Subjects stretching routine and a few high-intensity sprints, all
subjects underwent three trials (two times round the
Seven male amateur soccer players volunteered in this circuit for each trial) at three different intensities:
study. The players trained with the team (sixth division moderate, high and very high (maximal). To modulate
of the Italian soccer league) twice a week, plus the intensity the subjects were asked to use their perceived
weekly soccer game. Their characteristics are shown in exertion quantified at the end of every intensity trial
Table 1. They were fully informed of the aims and the using the Borg scale (0–10 point scale). In order to
procedures of the study and signed an informed consent obtain correct feedback from the players, they were
form. The study was approved by the Ethical Committee previously instructed in how to use the scale properly.
of the University of Milan. The experiments complied Between each trial, athletes rested for 10 min. During
with the current laws of the country in which they were this phase, capillary blood samples (5 ll) were col-
performed. lected from the ear lobe and analysed using a portable
The subjects were instructed not to eat for at least 3 h system (Lactate Pro 1710, Arkray, Japan), at minutes
before testing sessions. They were also asked to avoid 1, 3 and 5.
beverages containing caffeine for at least 8 h before Average data corresponding to the second lap of each
testing, and intense exercise in the previous 48 h. intensity trial were used to establish the individual
Tests were performed on the field and in the lab- average HR and V_ O2 values on the field.
oratory within 2 weeks. For each subject, field tests
always preceded the laboratory test in order to be able
to reproduce environmental conditions in the labora- Laboratory test
tory similar to those found on the field. During both
field and laboratory tests, expired respiratory gases The temperature of the laboratory was set at 28°C and
were measured using a breath-by-breath automated relative humidity at 60–70% in order to reproduce an
portable gas analysis system (K4 b2, Cosmed, Italy). ambient condition similar to the outdoor field tests.
Flow, volume and gas concentrations were calibrated After 10 min of warm up, the subjects performed an
before each test using standard procedures. The very incremental exercise on a treadmill (770S, RAM, Padua,
Italy) at a constant slope of 1%. Initial speed was set at
9 km h)1 and was increased by 3 km h)1 after each step.
Table 1 Characteristics of the soccer players [mean (SE)]
Each workload lasted 5 min and a resting period of at
Age (years) 25.3 (1.2) least 5 min was allowed between two subsequent steps.
Stature (m) 1.76 (0.03) During the recovery phase, capillary blood samples
Body mass (kg) 72.9 (2.1) (5 ll) were collected from the ear lobe at minutes 1, 3
Body fat (%) 8.1 (2.2)
Experience (years) 13.7 (2.3)
and 5, and analysed using the same portable system as
that used on the field. The maximal oxygen uptake
169

Fig. 1 Soccer-specific circuit used in the present study (modified regression equations obtained in laboratory. The esti-
from Ekblom 1988). Arrows indicate the direction of the movement mated V_ O2 was then compared to the measured V_ O2
during the exercise. Open circles The part of the track where ball
was involved (passes, kicks, dribbling, etc.), filled circles reference utilizing a two-way ANOVA for repeated measure-
cones positioned on the field. Dashed lines The position where the ments. When a statistical difference was detected, a
intensity of the exercise slowed down (jogging or walking, at an Bonferroni post-hoc test was applied. The level of sig-
intensity corresponding to the level of the exercise: moderate, high nificance was set at P<0.05.
or very high)

(V_ O2max) criteria were: (1) a plateau in V_ O2 (change Results


<2.1 ml kg)1 min)1), (2) peak blood lactate concen-
tration above 8 mmol l)1, or (3) a respiratory exchange In Fig. 2, HR and V_ O2 data from a representative
ratio (R) above 1.15 (Duncan et al. 1997). Average data subject during the field test at three different exercise
from the last minute of each step were used to determine intensities are shown. For our purposes, average data
the individual HR and V_ O2 values. from the whole second lap were utilized.
The mean durations of the moderate, high and very
high-intensity trials were 411 (14), 326 (9) and 266 (4) s,
Statistical analysis respectively. During the field exercise at moderate
intensity, average HR and minute ventilation (V_ E) were
Data are presented as means (SE). Before using para- 156 (4) beats min)1 and 68 (3) l min)1, respectively. The
metric tests, the assumption of normality was verified V_ O2 reached at this level of exercise was 2.49
using the Kolmogorov–Smirnov test and Lilliefors (0.05) l min)1, and the corresponding carbon dioxide
probabilities. For each subject, the linear regressions of production (V_ CO2) was 2.50 (0.07) l min)1. At the end
V_ O2 versus HR obtained in the laboratory and during of this exercise, lactate concentration [La)] was 1.9
the field test were determined. Group regression analyses (0.2) mmol l)1, with a mean rating of perceived exertion
were not performed as this statistical approach could (RPE) of 2.3 (0.2).
mask the individual relationships (Swain and Leutholtz At high intensity, HR and V_ E were 173 (5) beats -
1997). For each regression the slope, intercept and min)1 and 96 (5) l min)1, respectively. Average V_ O2 and
Pearson’s product-moment correlations were deter- V_ CO2 were 3.08 (0.09) l min)1 and 3.31 (0.13) l min)1,
mined. Paired t-tests were used to compare the slopes respectively. [La)] at this intensity was 4.4 (0.5) mmol
and intercepts of the HR–V_ O2 individual regression l)1, with a mean RPE of 4.4 (0.2).
lines obtained in the two conditions (laboratory and Lastly, at very high-intensity exercise, metabolic and
field). From the average HR data recorded during the cardio-respiratory values are presented in Table 2.
second lap of every intensity field trial, the correspond- Briefly, HR reached average values of 186 (6) beats -
ing V_ O2 was estimated using the individual HR–V_ O2 min)1 and average V_ O2 was 3.49 (0.29) l min)1. [La)]
170

Fig. 2 Example of raw data


from a typical subject obtained
during the field test. Grey area
The range of time (second lap)
from which heart rate (HR) and
oxygen uptake (V_ O2) values
were averaged to determine the
HR–V_ O2 relationship during
each exercise intensity

reached values of 13.4 (0.1) mmol l)1, with a mean RPE


of 9.1 (0.4). Discussion
During field tests at moderate, high and very high
intensity, HR was 82 (2), 91 (1) and 97 (1)%, respec- The main finding of the present study is that the rela-
tively, while V_ O2 was 70 (2)%, 85 (2)% and 94 (2)%, tionship between HR and V_ O2 determined in amateur
respectively, of HR and V_ O2 at maximal exercise in the players during soccer-specific exercises, simulating a
laboratory (see Fig. 3). training circuit suggested in the literature (Ekblom
During the incremental maximal tests on the tread- 1998), is not statistically different from that found in the
mill, all the subjects met at least one of the V_ O2max laboratory. Consequently, from the average HR re-
criteria (Duncan et al. 1997). The set of data used to corded during soccer activities, it seems to be possible to
calculate the regression line of the HR–V_ O2 relationship estimate the corresponding V_ O2 by using the HR–V_ O2
determined in the laboratory was averaged from the last relationship determined in the laboratory.
minute of each step. Maximal values reached during the For this study, with the aim to reproduce all the
laboratory test were not statistically different from those movement patterns and activities commonly performed
found on the field, as shown in Table 2. during soccer training or games, we modified the circuit
The two average regression lines obtained from the proposed by Ekblom (1998) by adding walking, kicking
individual HR–V_ O2 regressions determined on the field and ball passes, which were not included in the original
and in the laboratory are shown in Fig. 4. The mean circuit. In this modified circuit, soccer players were
value of the HR–V_ O2 regression line slope obtained in asked to perform three trials at different intensities
laboratory [0.030 (0.002)] was not significantly different (moderate, high and very high). Each trial was carried
compared to that found on the field [0.032 (0.003)]. out twice to reach a minimum of about 4 min of exercise
Similarly, the mean value of the HR–V_ O2 regression duration. Then, the individual HR–V_ O2 regression lines
line intercept obtained in the laboratory [79.6 (4.6)] was from the field data were determined using the second lap
not significantly different compared to that found on average values, when HR and V_ O2 data reached almost
the field [76.7 (9.7)]. The mean correlation coefficients constant average values (see Fig. 2). In this way, using
of the HR–V_ O2 obtained in laboratory and on the field the mean HR and V_ O2 of the whole second lap, we
were 0.984 (0.012) and 0.991 (0.005) (P<0.001), included several factors supposed to affect the HR–V_ O2
respectively. linearity (intermittent nature of the effort, sprint,
jumping and static contractions).

Table 2 Maximal values relative to field (second lap of the very


high intensity trial) and laboratory tests. V_ O2 Oxygen uptake,
V_ CO2 carbon dioxide production, V_ E minute ventilation, R respi-
ratory exchange ratio, RR respiratory rate, HR heart rate, [La) ]
lactate concentration. Data are expressed as means (SE)

Field Laboratory

V_ O2 (l min)1) 3.49 (0.11) 3.74 (0.17)


V_ O2 (ml kg)1 min)1) 48.1 (2.1) 51.7 (2.4)
V_ CO2 (l min)1) 4.14 (0.17) 4.18 (0.21)
V_ E (l min)1) 147.0 (14.5) 144.5 (5.8)
R 1.19 (0.01) 1.12 (0.02)
RR (breathsÆmin)1) 58 (2) 58 (2)
Fig. 3 HR and V_ O2 average values determined on the field at the HR (beatsÆmin)1) 186 (6) 191 (4)
different exercise intensities as a percentage of the maximum HR [La)] (mmol l)1) 13.4 (0.6) 13.5 (0.8)
and V_ O2 values measured during the test on the treadmill
171

Hoff et al. (2002), the incremental test protocol


(1 km h)1 every minute) was probably too short to
validate the V_ O2 estimation from the HR recorded
during soccer activities, and to compare the HR–V_ O2
relationship found on the field to that found in the
laboratory (Ekblom 1986; MacLaren et al. 1988; Rohde
and Espersen 1988; Van Gool et al. 1988; Bangsbo
1994a, b). Indeed, constant work-loads of at least 4 min,
as adopted in ours as well as in previous studies, and not
an incremental protocol as in Hoff’s study (Hoff et al.
2002), would be required in order to reach a steady-state
condition and to establish the HR–V_ O2 relationship
(Astrand and Rodahl 1977). Moreover, Hoff et al.
(2002) concluded that HR monitoring is a valid measure
Fig. 4 Scatter plot of the HR versus V_ O2 data obtained from tests of the exercise intensity during soccer-specific training
in the laboratory (dashed line) and on the field (continuous line). The situations by plotting only one experimental point cal-
regression equations represented in the figure were calculated using culated from a specific maximal field test over the HR–
the average slopes and intercepts of the individual regressions of V_ O2 regression line obtained in the laboratory.
the seven amateur soccer players
On the other hand, Tumilty (1993) suggested that HR
may not correctly reflect V_ O2 during soccer activities, as
The average V_ O2 values, measured on the field at intermittent or variable speed activities typical of soccer
each exercise intensity, were not statistically different may elevate HR disproportionately to V_ O2. Moreover,
from those estimated from the HR–V_ O2 relationship Ogushi et al. (1993) used the average HR and V_ O2
obtained in the laboratory (see Fig. 5). This finding measured on the field during a 90-min simulated friendly
confirms the validity of HR monitoring to determine the game, and compared the results with the HR–V_ O2
metabolic demands during soccer activities, at least in relationship determined in the laboratory. They found a
the range of the investigated intensities on the field [80– difference of about 25% between estimated and mea-
97% of laboratory heart rate maximum (HRmax) cor- sured V_ O2 and concluded that the discrepancy was
responding to 70–94% of V_ O2max]. These values are mainly due to the different V_ O2 response during inter-
similar to those reported in the literature relative to mittent soccer activities compared to the steady-state
actual games, when 80–90% of HRmax and 70–75% of laboratory test. However, the use of Douglas bags for
V_ O2max were measured (Ekblom 1986; Bangsbo 1994a, expired gas collection could have limited the normal
b; Felci et al. 1995). movement of the players because of the weight (1.2 kg)
The results of this investigation seem to be similar to and size of the equipment. Moreover, the lack of the
those found by Hoff et al. (2002) in six professional laboratory test protocol information, the small number
soccer players, when the average V_ O2 values measured of subjects (only two players) and the short length of the
during a dribbling track and a small-group play (five vs gas collection time (2–3 min each half-time) make the
five) were close to the HR–V_ O2 regression line deter- interpretation of their results difficult. Ogushi’s (Ogushi
mined during a treadmill test. However, in the study of et al. 1993) conclusion is also not in agreement with the
study of Drust et al. (2000), which showed similar
average HR and V_ O2 during intermittent exercise,
reproducing a soccer game and continuous running at
the same average speed on a treadmill.
Among other factors that could influence the HR–
V_ O2 relationship, thermal and emotional stresses have
been proposed, particularly during real competitive
games. Despite field tests being performed under hot
and humid conditions, the laboratory temperature and
humidity were set accordingly in order to reproduce a
similar milieu. However, although field tests were car-
ried out under occasionally sunny conditions, it is
possible that high environmental temperature influence
on HR was counterbalanced by the effect of wind and
Fig. 5 Comparison between V_ O2 estimated from the laboratory air exposure on body temperature regulation. Unfor-
test and V_ O2 measured during the soccer circuit at different exercise tunately, the design of the present study did not allow
intensities (n=7). No significant interaction between the two us to obtain more insights into the effects of emotional
measurement procedures (estimated vs measured) was found. A
Bonferroni post-hoc test revealed significant differences between stress on the HR–V_ O2 relationship. Indeed, our results
moderate versus high, high versus very high and moderate versus from the field were obtained during soccer-specific
very high exercises, where stress plays a minimal role compared
172

to real competitive games. It should be also taken into experimental evidence on the importance of HR moni-
account that stress can influence cardiovascular toring during specific activities coupled to laboratory
parameters at rest and during mild exercise, with neg- tests, trainers and coaches will be encouraged to use a
ligible effect at high and very high intensities of exer- more scientific approach to sports.
cise. This aspect of the study, though, may require
further investigation. Acknowledgements The authors wish to acknowledge Emiliano C’è
Even anaerobic contribution should be taken into and Marcello Iaia for their technical assistance in data collection
during the experiments. We also thank the soccer players involved
account as a factor affecting metabolic demands. HR is in this study for their committed participation.
commonly a good indicator of aerobic metabolism, but
it cannot reflect anaerobic contribution. In our investi-
gation both peak [La)] and V_ E (Table 2) reached during References
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