Lahore Grammar School

42-B/III Gulberg III, Lahore

Name:__________________ Grade: 8 Sec: ___ Date: _________________

Microscope Resource

Eyepiece
Look through the eyepiece to see the
image. Objective lenses
Check the number on the side of the Turn one of the lenses until it
eyepiece to see by how much it clicks into place. Always start
magnifies. with the lowest magnification
X10 = ten times magnification first.
The magnification is shown on
the side of the lens (usually x4,
x10 and either x20 or x40).
Focusing
Turn the coarse focusing
wheel or ring until the slide is
almost in focus. Use the fine
focus to obtain a sharp image.
Stage
Use the clips to hold
the glass slide in
Mirror place. Make sure
Angle the mirror until a bright that the cover slip is
circle of light bounces through facing upwards.
the glass slide on the stage.
Some microscopes will have a
built-in electric light to illuminate
the object on the slide.
Magnification
Total magnification = eyepiece magnification × objective lens magnification

Setting up the microscope

1. Place a glass slide on the stage.
2. Turn the objective lenses so that the one with the lowest
magnification clicks into place over the slide.
3. Adjust the mirror so that light passes through the slide and into the
lens.
4. Look at the slide from the side of the stage. Turn the coarse focus
wheel anti-clockwise to move the lens down so that it is just above
the slide. Take care not to hit the slide with the objective lens.
5. Look down the eyepiece and turn the coarse focus clockwise so that
the lens moves away from the slide until the slide looks as if it is in
focus.
6. Increase the magnification by turning the objective lens until it
clicks into place. Do not change the focus as the slide should be
nearly in focus. Look down the eyepiece lens and make any fine
adjustments with the fine focus wheel.