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Gameti
M.Sc. Agri. GPB 3rd sem
Reg. No.: 04-AGRMA-01998-2019
An Assignment on
DNA modifying enzymes
Submitted to:
Dr. H. N. Zala
Assistant professor,
Dept. of Genetics & Plant Breeding,
C. P. College of Agriculture, S.D.A.U., S. K. Nagar
WHAT IS AN ENZYME?
Bal31
• It removes nucleotides from
both the sides of the double
stranded DNA.
• It is purified from the
bacterium Alteromonas
espenjiana.
Exonuclease III
• It degrades just one strand
of double stranded DNA
molecule and leave single
stranded DNA as the
product.
2). Endonuclease
• It is able to
break internal
phosphodiester
bond of the
DNA molecule.
S1 endonulease
• It cleaves only single strand.
• It is purified from the fungus Aspergillus oryzae
Deoxyribonuclease 1 (Dnase 1)
• It cleaves both single stranded and double stranded DNA.
• It is purified from cow pancreas.
Restriction Endonuclease
This type of enzymes cleaves specific recognition site
on double stranded DNA.
PvuI : It cuts DNA only at the hexanucleotide
CGATCG
• This enzyme isolated from Proteus vulgaris
1. Blunt ends.
2. Sticky ends
1. Blunt ends
• In dsDNA, cut produce by restriction endonuclease
enzyme in middle and does not remain any terminal
sequence here. This ends are known as blunt ends.
2. Sticky ends
• In dsDNA, cut produce by restriction endonuclease
enzyme on both the srands. Two over hangs (extra
nucleotides are present) will be produce on the both ends.
This ends are known as sticky ends.
Categorization of enzymes
• Isoschizomers
Restriction enzymes that recognize the same sequence and produce cut in
same location.
e.g. sph-I & Bbu-I CGATAC/G
• Neoschizomers
Restriction enzymes that recognize the same sequence but produce cut in
different location.
e.g. sma-I [GGG/CCC] & Xma-I [G/GGCCC]
• Isocaudomers
Restriction enzymes that recognize slight different sequence but produce
cut in same location.
e.g. Sau3A & BamHI Both gives GATC sticky ends.
THANK YOU..