Phytotoxic Potential of Mangifera indica Extracts to Cyperus rotundus and Capsicum

annuum Germination

Ryza Jamie Sabater

Polangui General Comprehensive High School

Submission in partial fulfillment of the requirements in Research IV

November 2018

Mrs. Rachelan Buenaventura

 PHYTOTOXIC POTENTIAL OF MANGIFERA INDICA EXTRACTS TO CYPERUS
ROTUNDUS AND CAPSICUM ANNUUM GERMINATION
SABATER, Ryza Jamie S.

Mango leaves are potential bioresources due to their capability to produce

allelochemicals that can inhibit weed development. However, studies on the comparative effects

of this home garden tree on a food crop and weed are rarely conducted. The study was conducted

to determine the inhibitory effect of the aqueous extracts of Mangifera indica leaves to the

germination of Cyperus rotundus tubers and Capsicum anuum seeds, in terms of root and shoot

lengths of the tubers, and radicle and coleoptile lengths of the seeds. The leaves were

homogenized and soaked for 48h; the filtrates were used to prepare three treatments each set in

which it contained 5, 15 and 25% extracts. After 9 days of germination, the leaf extracts were

found to have both stimulatory and inhibitory effect on the tubers and seeds. The best treatment

on all sets was Treatment 3, which was the 25% mango leaves extract. In Set A, the average root

and shoot lengths the tubers in treatment 3 were 0.605 and 0.279, respectively. In set B, the

average radicle and coleoptile length of the seeds were 0.195 and 0.027, respectively. In Set C,

0.420 and 0.370 for the root and shoot length, and 0.500 and 0.577 for the radicle and coleoptile

length. The results in treatment 3 showed near result of the positive control at almost zero. The

researcher therefore concluded that the dry leaves extract of Mangifera indica possessed a

phytotoxic potential on the germination of Cyperus rotundus and Capsicum annuum.

 ABSTRACT

TITLE: PHYTOTOXIC POTENTIAL OF MANGIFERA INDICA

CATEGORY
EXTRACTS TO CYPERUS ROTUNDUS AND CAPSICUM ANNUUM
GERMINATION
Pick only one
NAME: RYZA JAMIE S. SABATER and mark an
Address: Polangui, Albay “X” in the box
School: Polangui General Comprehensive High School at its right.
Polangui, Albay

Life Science X
Mango leaves are potential bioresources due to their capability to
produce allelochemicals that can inhibit weed development. However,
studies on the comparative effects of this home garden tree on a food crop
and weed are rarely conducted. Applied
Science
The study was conducted to determine the inhibitory effect of the
aqueous extracts of Mangifera indica leaves to the germination of
Cyperus rotundus tubers and Capsicum anuum seeds, in terms of root and
shoot lengths of the tubers, and radicle and coleoptile lengths of the Robotics
seeds.

The leaves were homogenized and soaked for 48h; the filtrates
were used to prepare three treatments each set in which it contained 5, 15
and 25% extracts. After 9 days of germination, the leaf extracts were
found to have both stimulatory and inhibitory effect on the tubers and
seeds. The best treatment on all sets was Treatment 3, which was the 25%
mango leaves extract. In Set A, the average root and shoot lengths the
tubers in treatment 3 were 0.605 and 0.279, respectively. In set B, the
average radicle and coleoptile length of the seeds were 0.195 and 0.027,
respectively. In Set C, 0.420 and 0.370 for the root and shoot length, and
0.500 and 0.577 for the radicle and coleoptile length. The results in
treatment 3 showed near result of the positive control at almost zero.

The researcher therefore concluded that the dry leaves extract of

Mangifera indica possessed a phytotoxic potential on the germination of
Cyperus rotundus and Capsicum annuum.

RYZA JAMIE S. SABATER

Signature
 ABSTRACT

Mango leaves are potential bioresources due to their capability to produce

allelochemicals that can inhibit weed development. However, studies on the comparative effects

of this home garden tree on a food crop and weed are rarely conducted.

The study was conducted to determine the inhibitory effect of the aqueous extracts of

Mangifera indica leaves to the germination of Cyperus rotundus tubers and Capsicum anuum

seeds, in terms of root and shoot lengths of the tubers, and radicle and coleoptile lengths of the

seeds.

The leaves were homogenized and soaked for 48h; the filtrates were used to prepare three

treatments each set in which it contained 5, 15 and 25% extracts. After 9 days of germination, the

leaf extracts were found to have both stimulatory and inhibitory effect on the tubers and seeds.

The best treatment on all sets was Treatment 3, which was the 25% mango leaves extract. In Set

A, the average root and shoot lengths the tubers in treatment 3 were 0.605 and 0.279,

respectively. In set B, the average radicle and coleoptile length of the seeds were 0.195 and

0.027, respectively. In Set C, 0.420 and 0.370 for the root and shoot length, and 0.500 and 0.577

for the radicle and coleoptile length. The results in treatment 3 showed near result of the positive

control at almost zero.

The researcher therefore concluded that the dry leaves extract of Mangifera indica

possessed a phytotoxic potential on the germination of Cyperus rotundus and Capsicum annuum.
 AKNOWLEDGEMENT

. This page is dedicated for those people who supported the researcher until he finally

finished the study and claimed success.

The researcher would like to express her deepest and warmest gratitude for those who has

been with her all throughout the process in completing the study, even after every struggle she

has to face to complete it, they were there to lend her a hand and never gave up on aiding him.

The reason for her everyday life and happiness, the Almighty Father, the researcher is

greatly thankful for all the guidance you gave her all throughout the study, you never stopped

guiding her and supporting her to be focused and determined in finishing her study.

To the Parents of the researcher, she is also very thankful for being with her on every

step of the way, most especially for giving her financial support to reach her aim to make the

study successful.

Also, to the efforts of Malou Gleponio. The researcher gives her gratitude for helping

her in setting up the experiment. Without you, the study won’t be a success and all efforts will go

to waste, the researcher also appreciates your support and pieces of advice to make the study

more functional and useful.

To the researcher’s adviser, Ma’am Rachelan Buenaventura. May God give you more

blessings for all the help you have done to make the researcher’s study useful and functional. A

million thanks for the support you gave to her during the development of the study.
 And for the Classmates and Friends of the researcher, she is truly grateful for giving her

relief and happiness whenever the researcher feels stressed out of all the work and making sure

her smile will never disappear.

And to all other people involved in making this success possible, the researcher proudly

thanks you all for your endless efforts and assistance.

Thank you and may God Bless you.

The Researcher,

Ryza Jamie Sabater

 TABLE OF CONTENTS

PRELIMINARIES PAGE

Title Page……………………………………………………………………………….i

Abstract………...………………………………………………………………………ii

Table of contents…………….………………………………………………………...iii

THE PROBLEM

Introduction………………………………………………………………………….…1

Statement of the Problem………………………………………………………………5

Statement of the Hypothesis…………………………………………………………...6

Significance of the Study……………………………………………………………...6

Scope and Limitations…………………………………………………………………7

METHODOLOGY

Methods……………………………………………………………………………….8

Flow Chart…………………………………………………………………………….11

RESULTS AND DISCUSSIONS

Data Analysis…………………………………………………………………………12
CONCLUSION AND RECOMMENDATIONS

Conclusion…………………………………………………………………………..20

Recommendation…………………………………………………………………....21

BIBLIOGRAPHY

APPENDICES

RESEACHER’S BIO-DATA
TABLES

Root Length of Cyperus rotundus tubers (Set A).………………….………..…………..14

Shoot Length of Cyperus rotundus tubers (Set A) .…………………………………………..14

Radicle Length of Capsicum annuum seeds (Set B) ………………………...….………15

Coleoptile Length of Capsicum annuum seeds (Set B)……………………………….…15

Root Length of Cyperus rotundus tubers (Set C)……………………….…...…………..17

Shoot Length of Cyperus rotundus tubers (Set C) ………………………………....………..17

Radicle Length of Capsicum annuum seeds (Set C) …………………………….…...…18

Coleoptile Length of Capsicum annuum seeds (Set C)……………………………….…18

GRAPHS

Average Root and Radicle Length (Set A and Set B)…………………………………..16

Average Shoot Length and Coleoptile Length (Set A and Set B)………………………16

Average Root Length and Radicle Length (Set C)………………………….……..……19

Average Shoot Length and Coleoptile Length (Set C)……………………………….…19