November 3-12, 2021

Name: JULIANNE M. MERCOLITA Course Yr. and Sec.: BSED-SCIENCE 2B

Activity 9
Dihybrid Inheritance: Gene Interaction

1. Flower color in a particular plant may be purple, red or white, and these traits are
known to assort independently of each other. Homozygous stocks of each of these
colors were raised, crossed in the following fashion, and produced the following results;

Cross 1: P1 purple x red

F1= all purple
F2= 3/4purple:1/4red
P AA x aa
Gametes A a
F1 Aa x Aa
F2
A a
A AA Aa
a Aa aa

Cross 2: P1 purple x colorless (recessive epistasis)

F1= all purple
F2 = 9/16 purple:3/16 red:4/16 colorless
P AABB x aabb
Gametes AB ab
F1 AaBb x AaBb
F2
AB Ab aB ab
AB AABB AABb AaBB AaBb
Ab AABb AAbb AaBb Aabb
aB AaBB AaBb aaBB aaBb
ab AaBb Aabb aaBb aabb

Cross 3:P1 purple x colorless (dominant epistasis)

F1= all purple
F2=12/16 colorless:3/16 purple:1/16 red
P AABB x aabb
Gametes AB ab
F1 AaBb x AaBb
 F2
AB Ab aB ab
AB AABB AABb AaBB AaBb
Ab AABb AAbb AaBb Aabb
aB AaBB AaBb aaBB aaBb
ab AaBb Aabb aaBb aabb

Using the first two letters of the alphabet give the genotype/s of the following:

Cross 1 Cross 2 Cross 3

a. P1 AA x aa AABB x aabb AABB x aabb
b. F1 Aa x Aa AaBb x AaBb AaBb x AaBb
c. Purple F2 AA AABB AAbb
Aa AABb Aabb
AaBB
AaBb
d. Red F2 aa aaBB aabb
aaBb
e. Colorless F2 n/a Aabb AABB
aabb AABb
AaBB
AaBb
aaBB
aaBb

2. In cross 2 of problem 1, give the phenotype of the following genotypes:

a. AaBB = Purple
b. aaBB = Red
c. AaBb = Purple
d. AABB = Purple
e. aabb = Colorless

What is the progeny (cross 2) phenotypic ratio of the following crosses:

a. AaBB x aaBB = 8:8

AB aB AB aB
aB AaBB aaBB AaBB aaBB
aB AaBB aaBB AaBB aaBB
aB AaBB aaBB AaBB aaBB
 aB AaBB aaBB AaBB aaBB

b. AaBb x AABB = 100%

AB aB Ab ab
AB AABB AaBB AABb AaBb
AB AABB AaBB AABb AaBb
AB AABB AaBB AABb AaBb
AB AABB AaBB AABb AaBb

c. Aabb x AaBb = 7:6:3

Ab ab Ab ab
AB AABb AaBb AABb AaBb
aB AaBb aaBb AaBb aaBb
Ab AAbb AaBb AAbb Aabb
ab Aabb aaBb Aabb aabb

2. It was reported that in Japanese morning glory, Pharbitis nil, purple flower color may be
caused by dominant alleles at either one of two separate gene pairs e.g. A_bb or aaB_.
When dominant alleles are present at both gene pairs A_B_, the flower color is blue,
and when both are homozygous recessive, aabb, the color is scarlet. A blue F 1 was
therefore produced by crossing two different purple types AAbb x aaBB.
A_bb or aaB_ = purple
A_B_ = blue
aabb = scarlet
P AAbb x aaBB
Gametes Ab aB
F1 AaBb x AaBb

a. What is the phenotypic ratio of a cross of F 1 and either one of the parent.
AaBb x AAbb = 8:8
AB Ab aB ab
Ab AABb AAbb AaBb Aabb
Ab AABb AAbb AaBb Aabb
Ab AABb AAbb AaBb Aabb
Ab AABb AAbb AaBb Aabb
AaBb x aaBB = 8:8
 AB Ab aB ab
aB AaBB AaBb aaBB aaBb
aB AaBB AaBb aaBB aaBb
aB AaBB AaBb aaBB aaBb
aB AaBB AaBb aaBB aaBb

b. What is the phenotypic ratio of an F 1 x F1 cross?

AaBb x AaBb = 9:7
AB Ab aB ab
AB AABB AABb AaBB AaBb
Ab AABb AAbb AaBb Aabb
aB AaBB AaBb aaBB aaBb
ab AaBb Aabb aaBb aabb