Comparative test report of brakishwater fish culturing pond after post trial of Aquasan:

Parameters Pond -1(Control) Pond-2(Test=Aquasan Use)

Water Type Brackish Brackish
Pond Area 4 Bigha 1 Bigha
Water Depth 3ft 6-7 ft
Water Color Muddy Green Greenish
Culture Species Shrimp (P. monodon), Crab (Scylla serrata) Tilapia, Scampi, Seabass, Mullets
Biochemical test
Parameters Initial After 7th day Initial After 7th day of
Aquasan post trial
pH 7.6 8.4 8.2 8.8
Dissolve oxygen(ppm) 6 4 6.5 5
Ammonia content(ppm) 0.25 0.25 0.25 Not detected
Salinity(ppt) 2 2 1 1
Nitrite(ppm) Not detected Not detected Not detected Not detected
Microbial analysis
Initial After 7th day Initial After 7th day of
Aquasan post trial
Total bacterial count of water(cfu/ml) 8.4˟10⁷ 7.9˟10⁷ 7.2 ˟10⁷ 1.3˟10⁷

Total bacterial count of soil(cfu/gm) 6.2 ˟10⁷ 8.6˟10⁷ 9.3 ˟10⁷ 2.6˟10⁷

Total fungal count of water(cfu/ml) 0.8˟10⁴ 1.3˟10⁴ 0.6˟10⁴ 0.2˟10⁴

Total fungal count of soil(cfu/gm) 1.7 ˟10⁴ 1.9˟10⁴ 1.4 ˟10⁴ 0.7˟10⁴

Tab.- 1. Bacterial load in Pond – 1 and 2 at a time interval of 7 – days.

 Total bacterial count of water Total bacterial count of soil
10 10
8 8

cfu (10⁷)

cfu (10⁷)
6 6
4 4
2 2
0 0
Day 0 Day 7 Day 0 Day 7

Time interval Pond 1(control) Pond 2(Aquasan used)

Pond 1(control) Pond 2(Aquasan used) Time interval

Total fungal count of water Total fungal count of soil

1.4 2
1.2
1 1.5
cfu (10⁷)

cfu (10⁷)
0.8
1
0.6
0.4 0.5
0.2
0 0
Day 0 Day 7 Day 0 Day 7
Pond 1(control) Pond 2(Aquasan used) Pond 1(control) Pond 2(Aquasan used)
Time interval Time interval

Fig. 1: Total bacterial and fungal count in water and soil.