Li@uizyme ALKALINE PHOSPHTASE imized IFCC Method ) Code_| Product Name Packie SOOT iquee Raine PRES [ToD TS0015 | Uguayme Aline Passhatase [5 x00 LS00%¢ [Ligue skaine Prasphaase | 10x00 mi LS0000 Ligsyme Akane Phasohatase | SOO I Intended Use Diagnasti reagent fr quantaive i vt determination fALPinhunanserumor plasma nia signieanee Human ALP consists of 9 greup of enzymes which hyolseahosphates at an alkane pl. ALP four in practealyaltissuesofthe body butin ign concentrations Inthe osteoblast eto, ve lant, ney testinal wall and fcating mammary glands. In adults the ALP normaly found culating nthe Seu age derived ‘rom th ver. In chen orinadeescents going though pubertal growth spurs, hese i an adtoralconrbvtin from one a this accounts forthe higher vere Interval orthese groups. Pregnancy aserases thenermal alee ALP levels ate often observed in bone dseace ot Wer dsease ving the ily tact the source of the Iseenzymelsnotapparenttrenestration of GT mayhelp diferentate between the two, 8 resed GGT In the presence of 9 rises AL? would suggest the vers the primarysource. Increasee ALP {usualy normal G6T} Is seen in (Osteomalaci and Rckets, primary hyperpavathyridsm with bone involvement, Pagets disease, secondary Carcinemsinbone ng some cate ofereegencssroms Inerensed eves ALP fsllymthtased Gis seenin cholestasis, hepatis, cites, space eecuyig lesions ‘malignancy wth bone over ivevement exact Dracuction. Low levels of ALP may be observed in endtans which cause atrestee bone grt or in hypephosohatasa Principe The metbed according t9 FCC rearimendaton. This retod utises trophy shosphate as tne subst Unger epinized conctions ALP present nthe sample caalysesthefolonngrencton np AMPrANNPSH ——e Avaphero phosphate Ng,./Akaine pH AL he pt ofthe tection, &ivaphene! has anintnse yellow colour The reagent also certains a metal onbufer systen te ensue that opin! concenratons of Zine are BEACON DIAGNOSTICS PVT. LTD. B BEACON Magnesium ae maintane. Themetal on bur can also ‘helote ater pocentiaty Ibn ln whieh may be present Thereacionsmenteredby measuingthe ate of Increase in absorbance at 405 or 415 rm which Is properional to the actvly ef ALP in the serum Reagentcompostion Reagentt: AMP Butter 2AMP 7250 ell. Mg:2 Sena In? stommeli HEA 15mm Reagent 2: PNPP Substrate Pavrophenyaresphate >16 mmo! Reagent Preparation Reagent que, rey to use stabity And Storage The unopened reagents are sabe til the existe tvlegonthebotlesnditbelnenstredat 2-8. Material RequreeButNotProvided lean ry contain Laveratr) ies PapetesorMcrapppetes & Tis Coluneteor Ble Chemistryanayer Wecking Reagent Preparation Mic peton of reagent Rt with portion af reagent 2 Sablty 5 days t15-25'Cin dark 30 aysat2-8Cin ear Specimen Caliectin And Honing Use serum psa Heparin Is ecommences to fol NCCLS procedures fr sina tangardzed condos Stab Serum Plasma: ‘hours st20-25¢ Bays aac 2vontns 20c Diseare contaminate specimens. usity contrat IRereconmenedtorunnermaland sbnormalcontlsers tovaldtereagenperermance Unitconversion UnxooT pat ExpectedVaues Chleren (2159) fo4-308u abit: 25-wou 424, EW GIDC, KABILPORE, NAVSARI 96 424, NDIAWisreconmendethateoeh bortonyveriy is range Asay ParametersForPetomeer xceivesretereceintenalfertepopuatentseves. aa Perirmance Dota Woveeng om 5 Data cata thin ts Scten representa of perternance en escent Sanple Vue i 2 Dun ebaines nour beer may er fam tese | Wert Rengentvoue Ta | 100 vats. Lagaine fe) 0 Limitotquntfeation = 100 UL Tieden bec) so Unesrty 4800 Ut Measurngronge 100-1600 Ne ational 2 Tete Fae 2768 or Foaoren + weninrn20) | wey | qu) | po z Somer eS) [emattow unr 25 Sie? eee] Demat 10 Tey Low w Tiecanaypecsen] Wen [sD [ev] | menmvioni) | unten inzoy | un | wy |r near oh "600 Sample? [065 [oes] | Beko Ware Comparison ut we AconatserbetneenBeacon AL Yfardaconmercily —Releences aladletest using 20sanplesgavefalowingresuts y = 1006x118 un r= ogee Interterences Follening substances dntintertre haemoglobin up to 5 gf, bliin up to 40 magi teers upto 2000 i WasteManagement Peas refertolcalegalregurerents Assay Procedure Wavelength 5 405nm cuvette 5 ten Teton Sequence Weuine Working Reagent 1000 Sime 204 ‘Mowe and read he iti abseraarce ater nates ne repeat the absorbance recing ater every 1.2.3 minute (alesse tinue absorbance change (Ani Catelton Using acon: APU) = fxBAmin ‘ factor ‘ 2764(3t 408 am} Applications for automatic analyser conrequest. 4Ziva JF, Banal PR, “Plasma Ena in Dagres in ‘ea! Chemisty In Oagress and Treatment. Leyes London 178: Chapter 18:38, 2uFCC mathe forthe mesure ALP Clin. Chem, ‘in Behe 1883.20 791.48 4. Young DS. Eects of Drugs o Cina Laboratory Tests The Eaton 980: 3:1925, “Ales Tertdook of Cinial Chemisty and Molecule Diagnoses Bus, CA, Ashwood ER, Bruns, DE: Sth ‘ation, WB Seances Carp. 207 SKapian and Pesce 4s) Cina! Chemisty, Taeary nays nd eonlton, Secon Een. CV Mosby C2 ‘0s, Sos Ved nab ey ction ute kona tl ts Cn Sm] lame fovse QZ boy bate [Ro] invie dagnostes