Li@uizyme a AMYLASE (Direct Substrate Method) Code_| Product Name Packie TSO03A | Tieuayme «Anse Tain £50038 | Liguayme a Anylase 7x20 i s003e| Liguzyme wAnyiase Sion £30030] Ligwayme a Anylase TOxtOm Lso03e | Ligwayme aAmyase Toxo S003 iguyne @ Amylase 1x00 TS0036|"Leszye a Amylase 7100 i TS003H| Leave a Anvase extn ‘Sona | Ligue @Anyase 420 Intendes Use Disghostereageat for evanttatve nwo determination efalphe-naseinumanserum pena nde Cina Signiteance «ysis cerved mony rom the slay lanes ane the exocrine pancreas. cAmyase cafases he yeas of e-4 glucoside nkages of starch and etre eatee pelysacchariges te preduce maltose and other eligosaccariges. The enzyme is 2 relatively small molecule which rapily cearee by the kidneys and ccreted in the urine. Anyiase is mest ‘requenty ease In the eagress of acute panceattis when sera eels may be goss eevate a aeatepancreatiis ceamyfase stars tore approximately &houts ae the lenst of pan reaches a peak at 24 hous and remains evatee fc 3.7 days. Hypranyasmia sas assecated ‘th ter ate addarina eras, bay aystunton, salary gland dsorers, ruptured ecopc pregnancy anc macoanylasons Principle aAmyase cates the hyehass of 22 chalet nite renal salt to care nite phenol (CNP). The tle of yeas measured as an nrese n absorbance due te ‘efomatanot ever nivophera whichis presteralts the anylaseatviyinthesarle. Resco aaryase eNpcaic2+H20 ————» eNP+ Gabe Reagent Compestion I= Amyiase Reagent Mesbuter >So Ctleum Chioide ay Reagent Preparation Reagent sigue, racy to se B BEACON ‘Stability Andstorage “he unopenes reagents ae stable tl the expiry date staledonthebote srt abelenstoreest2-#C Material Require But Not Provided Clean deycontainee ~ LaborateryclassPppetesor Wiropipgetes Ts. ColemetererBtoChenistyAnaijzer, ‘SpecinenCallectonAndHending Use serum pasa heparin EDTA ue Is tecommended to falaw NCCLS procedures fr senior standardized conditions} stbity InSerum/Prasma eays 120-25 Teays auc ‘year stare Inne Pays 3120-25" ‘days a-ec aes s20e Discard contained specimens ualty Control Itsreconmendedto run nomaland abnormal cenl ser tovaldatereagentgeronmance Untconverson Utx00T7=pau ExpectedVaues mre Serum: uptesoun Uiine —uptoato ut Ins ecommended that each laboratory verity tis range rdetverreferenceinteralforthepopulatonitzerves. Performance Date Data coraied wishin this secten Is representative of performance on Beacon system. Data obtained in you labratrymay terion thesevalus. Limi of quanteation Linesiy Measuring ange wou ‘600 ut 00~1500UL “BEACON DIAGNOSTICS PVT. LTD. 42, WEW GIDC, KABILPORE,WAVSARI- 986.428 NDIAPrecision -AssayPorametersForPhotometers Tava ay preciion] Mean |S] ev ede wine wiennni29 | ow | on | 6 | Cee 05 sre E Sample Vola 25 a sso | Reagent Velume i 1000 Imerassyprecson] Mean [SD "1 ¥ tag tine ee) a unternin=zoy | wn | wu | 09 eae = Sample 26 [ 083 [03 nse ele) Neotel 3 Comparison - A compaon between Beacon Anise yh and a | Met Fee a8 conmeraly aval tes) sing 20 samaies gave | Revelon temp 1) 7 folewingresuts Reaction Deecion inreasg y = 100% 08H0UR = ye oe eral tw ° eral gh oo Interferences —eeee Feleving substances denetitere: neato 6 haemoglobin upto 25 af blewbin upto 40 mais, [Thea Hh 7500 ‘igyeitestpte2000n9 To ier Note: i uw Sita ad stn coin aphoamyase eee never pte rogers by mouth an ave cotarinaton of References ‘pes ad regen TIF Ts, and BR. Paral, Plsma Eryn in ‘amples ad reagents. However tace contamination can svfectess Waring AnaPrecautons For invita cagnertc use. Tobe hard by ened snd protesanalyedueatedpesen Reagents ofthe tae net cassie the dangerous bat canfanieethan 09% sodium ande-castieg everyone anécangerus substance fare envrenment WasteManagement Peas refertolcalegalregurerets. AssayProceaure Wavelength 40500 cuvette ten Teor Sequence Veuune ‘Anyfase Reagent 1000 Sine Bul IM neubate 4 mint IPC and then esi thei sbrerance of clbvater and sample against reagent Dank, Measure the absrbance change exact afte 1,2 ‘nmin, Cleust minute sbeocbene change (Ami Caleulaton Using face: Amaya seo (Ul) =f BAIN ‘ = fecer ' 3178 fa 405 rr) Applletian for automatic analysers ae avaliable on request. Diagnesie” in Cina! Chemisty in Diagnass ane Treat loys angon 1879" Cnaplerxv 3612, 2.F09, Y. Rand Brosh, §. 6. Ann. Cin, Bachem, 906,23.60437 3.Bais RAJ of Cin Path 1982; 78: 1848 ‘cna, Chemisty irfobas: &Scletic & Management ‘jlpeeaPesce-Kapanublhers 196, 268-2620, 5 shepherd, MDS, 2rd Mazzac, RD. The Cn Brecher, ‘983-4 617 6 Young DS. Etects of rugs on inal Laboratory Tests ThiveEion'960:3:346 17 Tiets Textbook of Cinial Chemisty and Molecule Diagnoses Burts, CA, Ashwoed, ER, Bruns, DE Sth ‘don, Wl Ssunders Carp. 2072 Wace, Meta Cresconandvertcaton of Referance intra. Labor‘ Medine 1985: 26.5831 Sos Ved nab ey ction ute kona tl ts Cn Sm] lame fovse [Ea] conten YZ Gorvose Eww] nv Dagrastes ele