ASO LATEX (Slide Agglutination Method) Code_| Product Name Paks SFomta| ASO Lex wT STO] ASO Latex 30 stoarc] ASO Latex 007 e020] ASO Latex 2507 ‘Quatatve determination fat strepteysin 0 (450) storest28C Principe o The Method he ASO lates asice aggutnatiotestfrthe qualitative snasemiquanttave dtecton of nt-teptoin (ASO) nun serum Latex parle coatee with stepoysin © (SLO) are sggithstedwhen meted samples containing ASO. Cina significance Sepleysin Os a Lore immunogenic exoenzyme produced by -heamotie Sueptoceccot groups, Cand 6, Measung the ASO antbodis ate useful fr the Aspro of neumstod fever. acate gemersoneatis ane steprococcal infections. Rheumatic ever Is an lngarmmatoyy cisease afecing connecive tissu fron several pats of human body a kin, Nea js, et.) ang acute glomeronephrits is 2 rena nfecten that affects maint renalglommers. Tater parties coated with steptaysin pHa, Presenatue Pove cont wi Latex Antigen Reagent 2 Posive Conrad preservative Negave con with presenatve agent Negative Conia) Precautions Components fam human etgh have Been testes ane foundte be negative forte presence of HBsAg, HCV. and antbedyteHHV2 Heweverhandecautousiyaspetenty infectious Caliration The ASO tex senstity i clbrated aginst the ASO International tandarttom NBSC871652 StorageAndstabilty ‘te hi corponent are ready to ure, and wl remain stad euntithe expiration date prntedon he abe when B stored tity cosed at 28°C and contarinations are prevents utng he use. De ot eeze: zen reagents feulsehangethelurclionaliyofthetest Always eau, Invericalpesten the postions change, genty mits sissveaggegatesthatmayaepresent Reagents deterioration: Presence of partes sre ‘uly. AddonalEauipment Mechanica eater ith austabe sped t 8100p Vortexnier Propees Sout ‘Samples Fresh serum, tale 7 ays 228°C or} months at 20°C Samples wh presence of fn shoud be centtuged Denetusehighyhemelederlipemic samples. Procedure (ualtative Method “Allow the reagents ane samples to reseh room temperature. The sent of the et may be ecuces atlowtomperatires 2PaceSDpL ofthe sample and ane cep ofeach Pesive find Negative centos inte separte cles 09 the sete 5. Nbxthe ASO atereagentigerousyorenavertox miter Beforeusng and add ene drop 50.) nettothesampe tobereste, “4M the crops wi asin, sprescing ther oer the lente surace ofthe cre. Use dterentstiners for acral. ‘Pace the slde ons mechanical eater £000 cpm, {or minutes, False postive result oud appeae i he testiseneiterthan wominutes Semi-quanttatveMethed ‘Make ser two fold kone ofthe sample 9 gf saline solu, Proceed foreach dition 3 nthe quale metned Reading an interpretation Famine mocresconcaly the presence oF absence of sible agglutination immediately ater removing the side fram thereto, ‘Thepresece of agpunatonincates an ASO concenrton quar retethan2001UnL The ter nthe sem quantitative rethe. is eine asthe Inghestallutn stowengapestiveresut “BEACON DIAGNOSTICS PVT. LTD. 42, WEW GIDC, KABILPORE,WAVSARI- 986.428 NDIACatelatons The apprexmate ASO cancentation the patient sample isealelatedefaows: 20x80 Tter=WUinL ‘uatty contro! Pasiive and Negotve conto: are recommended to moniter the prfrmance of the procedure, 35 well 5 2 ampatatveptiertorabeterresutietrpretaon ‘Ares ferent rom the negatve conzl su, willbe cansdered as postive, ReferenceValuee Up te 200 iWin fads) and 100 IU fhieren « 5 yearse. Exch laboratory soll establish is reference range Periormance Charsetrises { Anotyca! senstwty: 200 f= 50) Wil, under the feserbed assay onatons 2Prozone eect: No rezone eect was cetected up to 150010, 3 Diagnostic sensi 989. ‘4 Diagreste specifiy 97%. Interterences Babin 20 gle), hemogibin 10 gL), pies 10 gi ‘heunattéfactrs (300 Ui do net iter. Other substaneesmyintrre LUnitations of Me Procedure False postive ests maybe blaine incondtonssuch , heumaode at sce fve tonal, evra Steptoocclinfectns an heslby eres Ey infectene and eden from 6 rors te 2 years maycaus falseregatwerests 4 sgl ASO determination does net produce much infermaton about the actual slate of the seas, Tiratn a weet interals during 4 or 5 weeks ae usable to folaw the ease evolution, Cine tiagnose should pot be made on ndings of 3 single test result, but should Integrate beth lineal ane laboratory esta Biography 1 Hate Ovartery JuralefMedécine 882. New series 35;305 641658, 2.hmee Smet Pit nal 992: 24 835-002, 3 spa etal Bua Wid th Org 964 24:275278, ‘The association of Clinical Patholegsts 1961 Broosseet 34 5 PlareB etal La PresseMedicle 1983 22:26 6 KeinGe. Applec Microbiology 197121991001 Young 5. ets of rugs on cna aberatry test th AACE Press, 1995, Oil Sos Ved nab catgue ute kona tl ts Ser = ae aene fovse Bory Date [WO] Invine Dignostes lie] [EE