Li@uizyme ALKALINE PHOSPHATASE SINGLE REAGENT (AMP Optimized IFCC Method ) Code Produc Name Pak Sie Tiedje Akane Prospaase 0028 ‘Single Reagent aT Intendes Use Diagnoste reagent fr quanta nv determination efALP nnuransexum ple, Cina Signieance Human ALP consists of 2 group of enzymes which yoroyse phesphates a aang pH ound practically attissues of tre body butin high oncetrions Inthe osteoblasts eee, er alacenta, dre, tsi walla lactating mammary lanes. In agus the ALP ermal fund erulting the Sum is agely derives from ne ver. hire vin aeeescent going trough pubertal raw spurts, these an adltenalcontraution From bone ane this secouns for the higher rterence interval hese groups. Pregnancy aso ralsesthenerma values afALP. RaseaAL lees ate en obserwesindene dsease or iver esease invling the bila act. Ifthe source ofthe enzyme s nat apparent then estinain of {66T may hep iferenite between the two. Aised GST Inthe presence of raised ALP would suggest the ers the primar source erase ALP svaly nr 6ST) is seen th Osteomalacis and Rieke, priary hyper ‘thytissm vith Bone invlvement, Pages cisease, Secondary carcinoma in bone and seme cases of esteagen¢ sarcoma ceased ves fALP usually with raised GGT) is seen in cholestasis, hepatts, cress, Space ecuping esters ae malignancy wit one over invevementerdretprecucten. LowlevelsefALP-naybe shseredincondons whch cause aresteg bane grat exintypopbosphatasia Principle The metnd aecoring to FCC recommendation. This rmetod ubisesntopheny phosphate ashe substrate Under opiised conditions ALP present in tie sample cctalyezthe flowing ac. AMP+4NNP+HO —— 4aitoprero+ phosphate Mi, Akane pt [A te pli ofthe reaction, 4ttophenel has ainense yellow colour The reagent alo certansametalinbufe: System teense ta alia! concetaans af Zine an Magnesium ae maintained. The etalon bufer can a0 fhelte eter potently Ibery ins wien may be preven. Therescioniementoreaby measuring erste ot Incease in asoraance at 405 or 415 nm which is propororltthe acy ofAL? inte serum. 6) BEACON Reagent Composition Reagent :Alaline Phosphatase Reagent ZaMP 2250 mmol ag2 smn, Ino sammoti HEOTR | sme! Pwr: <10 mmo Reagent Preparation eager sau, ready use Stability And Storage The unepened reagents are stable til the expiry ate staledon nebo sre dele storedat2- °C Mater RequiredButNotProvided ‘lean &Drycentaner LaberataryGiassPopeteserMlcropppetes& Ts ColerietererBio-ChemistyAnayeer, Specimen olecionAnd Handing Use serum, sasma Heparin. tisrecemmendedtefllow NCCLS procecuresr similar standardized conditions Stabty Serum Plasma: ‘hour 3120-25 Seays strc Zens at20 Dscaracontinated speciens. ual Contra Iesteconmendedta run nermal and abnormal centile tovaldatereagenpecermance Unitconvesion Unsoot7=pkatn Expeced\alues Caren 8 97) 104.3090 Adults 25-WOUL IRierecommended that ech aboratory very this ange ‘derives referenceinterva forthe population serves PerformanceDats Dita contained within ths secton is represeratie of per‘omance on Beacon system. Data eatind in your loseratry may lifer om thesevalues, Limit of quanteation : 10.0UL LUnesiy 1600 UL Messuingrange 100-1500 Inve assay precision] Mean] SD] Wainrunio-20) | quay | wy | to Sanple [160 182 Sanplez we [ 524 [at BEACON DIAGNOSTICS PVT. LTD. 424, NEW GI00, KABILPORE, NAVSARI~ 986424. NDIAIntewassaypeeiion] Wean | SD] GV] References Runteran nez0) | quity | funy | cx) | 12a JF, Pana PR. “Plasma ExzynesinDiagnesis” in Tangle Geass] os] Mina Chemstry in Diagnosis and Treatment lye Lencen 7978: Chapter 15: 343, Comparison comparison setween Beacon AL? and scammer avalabletest using 20 samples gavefolevingresuts FCC method fr the measurement of ALJ Cl, Chem (in Bachem, 983-21 73148. 23 Young DS fecsof Drags on ClricalLaberatery Tests ye loves Terstnt90 9125 ‘es ero o inl Chen and ster iererencs Dagorer bu. €X Ane ER, BBE, 3 Fahmi esaces neiere schon Wns cong 202 Tarren up te Gl Don so t6 49 mal, apn and Pies er) ns! Chant, Te ojennernto20000961 tls coven Sean een Voy Co Waste Management 1989, Pere lcnepaesutenes Acsoyrceie wecena {050m oe ten Ten arn Teams Reiger 04H Some 2 Vows ndesdlbbneater tes and Ieee hence eng ter svy 2 3c Cate mintessoame tegen Calton Uang'ncar APR = fcaAnn ' te t Soha 5 soy or Pattee vee Tate Waco 55 Sane alr = Fever Ye i in Ta oe ee % Kinetic Interval (sec) « ‘Symbols Used On Labels Tee ena 3 Cuome ca vane Tete Facer 768 wnser ll scans TS 7 Ser = ae aene CH ieuse eration i 2s Tesi ® Treva a YZ Gorvose Eww] nv Dagrastes Saw Tier Is = = pee Estos [ESS