Senior High School

POLOMOLOK NATIONAL HIGH SCHOOL

Science, Technology, Engineering & Mathematics
Brgy. Cannery Site, Polomolok, South Cotabato
Tel. No. (083)887-5849, Fax (083)887-5849 e-mail:polnhs@yahoo.com

Chapter IV

RESULTS, ANALYSIS, AND INTERPRETATION OF THE DATA

This chapter compromised the presentation, analysis, and

interpretation of the data gathered after the final experimentation of

the study in terms of the phytochemical screening of the ethanolic

extract of White Radish (Raphanus sativus L.) and the antifungal

testing against Saprolegnia multispora fungus. The data are presented

in tabular form for clearer interpretation of data.

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 Senior High School
POLOMOLOK NATIONAL HIGH SCHOOL
Science, Technology, Engineering & Mathematics
Brgy. Cannery Site, Polomolok, South Cotabato
Tel. No. (083)887-5849, Fax (083)887-5849 e-mail:polnhs@yahoo.com

Phytochemical Screening of the Ethanolic Extract

Table 1. Presence of Phytochemical in the plant White Radish

(Raphanus sativus L.)
Secondary Metabolite Changes Formed Results

Alkaloids No changes formed -

Anthocyanosides No changes formed -

Flavonoid Golden Yellow color +

Brown ring formed in

Glycosides the interface +

Phenolic Compounds Black Color +

Saponins No frothing is formed -

Brownish-green

Tannins precipitate +

Legend: (+) indicates the presence; (-) indicates the absence

Table 1 shows the result of the phytochemical screening done

with the White Radish (Raphanus sativus L.) ethanolic plant extract.

Qualitative analysis showed positive result for Flavonoids, golden

yellow color, Glycosides; a brown ring formation in the interface,

Phenolic compounds, black color and Tannins, brownish-green

precipitate. However, it shows negative in Alkaloids, Anthocyanosides

and Saponins.

47
 Senior High School
POLOMOLOK NATIONAL HIGH SCHOOL
Science, Technology, Engineering & Mathematics
Brgy. Cannery Site, Polomolok, South Cotabato
Tel. No. (083)887-5849, Fax (083)887-5849 e-mail:polnhs@yahoo.com

Antifungal Screening of White Raddish (Raphanus sativus L.)

Table 2. Zone of growth inhibition of Saprolegnia multispora

fungus

R1 R2 R3
Treatment Mean (mm)
(mm) (mm) (mm)

T1– 100%
8 8 8 8
Plant Extract

T2– 75% Plant

7 8 7 7.3
Extract

T3 – 50%
6 6 5 5.7
Plant Extract

Table 2 shows the zone of growth inhibition of the three

treatments of every replication. Treatment 1 or 100% Plant Extract

inhibits the growth of the bacteria by 8 mm in all replications.

Treatment 2 or 75% inhibits the growth of the bacteria by 7.mm in

two replication and 8mm in one replication, and Treatment 3 or 50%

plant extract inhibits the growth of the bacteria by 6 in two replication

and by 5 mm in one replication.

48
 Senior High School
POLOMOLOK NATIONAL HIGH SCHOOL
Science, Technology, Engineering & Mathematics
Brgy. Cannery Site, Polomolok, South Cotabato
Tel. No. (083)887-5849, Fax (083)887-5849 e-mail:polnhs@yahoo.com

Table 3. Summary of One-Way ANOVA for Zone of Inhibition of

Saprolegnia multispora after 24 hours

Source of Sum of Degrees Mean Computed P-value Tabulate

Variation Squares of Squares F d F-

Freedom value

Between 2 0.444444 0.093023 0.912461 5.143253

0.888889
Groups

Within 28.66667 6 4.777778

Groups

Total 29.55556 8

Table 3 shows the summary of the ANOVA values of the zones of

inhibition of Saprolegnia multispora after 24 hours from treatment

application. The critical f-value 5.143253 is more than the computed

value which is 0.093023. Statistical Analysis showed that the

Saprolegnia multispora was not significantly affected by the different

concentration of white radish (Raphanus sativus L.) ethanolic extract.

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