UJI EFEKTIVITAS INFUS RUMPUT TEKI DAN PUTRI MALU TERHADAP BEBERAPA BAKTERI UJI (Perbadingan

sediaan Tunggal dan Kombinasi)

A. Staphylococcus aureus
No Perlakuan Jumlah bakteri Staphylococcus aureus
I II III IV V Rerata Standar
Deviasi
1 A (50%) 80 93 86 89 83 86,20 + 6,56
2 Rumput Teki B (75%) 76 89 85 81 86 83,40 + 5,02
3 C (100%) 65 64 71 63 70 66,60 + 3,64
4 Putri Malu D (100%) 79 67 80 74 77 75,40 + 7,23
5 A + D 51 52 49 51 50 50,60 + 1,52
6 Kombinasi B + D 48 44 35 38 46 42,20 + 6,65
7 C + D 24 23 21 21 23 22,40 + 1,52
8 Positif Alkohol 70% 20 24 20 24 22 22,00 + 2,00
Arsir kuning yang dimasukkan di tabel 1 jurnal lalu tambah notofikasi huruf kebermaknaan.
Nilai reraratanya dimuata gambar ya
Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Perlakuan_S.aureus Statistic df Sig. Statistic df Sig.
Koloni_S.aureus RT50%_S.aureus .136 5 .200 *
.990 5 .980
RT75%_S.aureus .225 5 .200 *
.958 5 .795
RT100%_S.aureus .270 5 .200* .860 5 .229
PM100%_S.aureus .220 5 .200* .888 5 .347
RT50% + PM100%_S.aureus .237 5 .200 *
.961 5 .814
RT75% + PM100%_S.aureus .228 5 .200 *
.925 5 .563
RT100% + .273 5 .200 *
.852 5 .201
PM100%_S.aureus
Alkohol70%_S.aureus .246 5 .200* .956 5 .777
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.
Koloni_S.aureus Based on Mean 3.322 7 32 .009
Based on Median 1.472 7 32 .212
Based on Median and with 1.472 7 20.447 .232
adjusted df
Based on trimmed mean 3.166 7 32 .012

ANOVA
Koloni_S.aureus
Sum of Squares df Mean Square F Sig.
Between Groups 23348.375 7 3335.482 209.944 .000
Within Groups 508.400 32 15.888
Total 23856.775 39
 Koloni_S.aureus
Duncana
Subset for alpha = 0.05
Perlakuan_S.aureus N 1 2 3 4 5 6
Alkohol70%_S.aureus 5 a21.80
RT100% + 5 a22.40
PM100%_S.aureus
RT75% + 5 b42.20
PM100%_S.aureus
RT50% + 5 c50.60
PM100%_S.aureus
RT100%_S.aureus 5 d66.60
PM100%_S.aureus 5 e75.40
RT75%_S.aureus 5 f83.40
RT50%_S.aureus 5 f86.20
Sig. .813 1.000 1.000 1.000 1.000 .275
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.

Huruf skrip nanti dimuat pada tabel 1 mendampingi nilai rerata+standar deviasinya ya.
B.Streptococcus epidermidis
No Perlakuan Jumlah bakteri Streptococcus epidermidis Standar
Deviasi
I II III IV V Rerata
1 A (50%) 82 84 86 83 84 83,50 ±1.48
2 Rumput Teki B (75%) 79 80 78 77 79 78,60 ±1.14
3 C (100%) 60 61 63 65 62 62,20 ±1.92
4 Putri Malu D (100%) 70 69 72 73 71 71,00 ±1.58
5 A + D 47 50 49 49 51 49,20 ±1.48
6 Kombinasi B + D 40 39 38 41 40 39,60 ±1.14
7 C + D 20 22 24 23 21 22,00 ±1.58
8 Positif Alkohol 70% 22 24 21 20 22 21,80 ±1.48

Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Koloni_S.epidermidis Statistic df Sig. Statistic df Sig.
Koloni_S.epidermidis RT50% ,246 5 ,200 *
,956 5 ,777
RT75% ,237 5 ,200 *
,961 5 ,814
RT100% ,141 5 ,200 *
,979 5 ,928
PM100% ,136 5 ,200 *
,987 5 ,967
RT50%+PM100% ,246 5 ,200 *
,956 5 ,777
RT75%+PM100% ,237 5 ,200* ,961 5 ,814
RTPM100% ,136 5 ,200* ,987 5 ,967
Alkohol70% ,246 5 ,200 *
,956 5 ,777
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.
Koloni_S.epidermidis Based on Mean ,242 7 32 ,971
Based on Median ,241 7 32 ,971
Based on Median and with ,241 7 30,322 ,971
adjusted df
Based on trimmed mean ,238 7 32 ,972

ANOVA
Koloni_S.epidermidis
Sum of Squares df Mean Square F Sig.
Between Groups 20694,375 7 2956,339 1321,269 ,000
Within Groups 71,600 32 2,238
Total 20765,975 39
 Koloni_S.epidermidis
Duncan a

Subset for alpha = 0.05

Koloni_S.epidermidis N 1 2 3 4 5 6 7
Alkohol70% 5 A 21,80
RTPM100% 5 A 22,00
RT75%+PM100% 5 B 39,60
RT50%+PM100% 5 C 49,20
RT100% 5 D 62,20
PM100% 5 E 71,00
RT75% 5 F 78,60
RT50% 5 G 83,80
Sig. ,834 1,000 1,000 1,000 1,000 1,000 1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
 C. Escherichia coli
No Perlakuan Jumlah bakteri Escherichia coli Standar
Deviasi
I II III IV V Rerata
1 A (50%) 89 103 97 95 96 96,00 ±5
Rumput Teki
2 B (75%) 87 96 94 90 95 92,40 ±3.78
3 C (100%) 74 72 81 76 75 75,60 ±3.36
4 Putri Malu D (100%) 85 82 86 89 80 84,40 ±3.50
5 A + D 53 52 50 51 50 51,20 ±1.3
Kombinasi
6 B + D 39 41 42 40 41 40,60 ±1.14
7 C + D 27 25 26 27 28 26,60 ±1.14
8 Positif Alkohol 70% 26 26 27 24 25 25,60 ±1.14

Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Perlakuan_E.coli Statistic df Sig. Statistic df Sig.
Koloni_E.coli RT 50% ,221 5 ,200* ,958 5 ,794
RT 75% ,264 5 ,200 *
,903 5 ,429
RT 100% ,253 5 ,200 *
,925 5 ,560
PM 100% ,168 5 ,200 *
,981 5 ,940
RT50%+PM100% ,221 5 ,200 *
,902 5 ,421
RT75%+PM100% ,237 5 ,200 *
,961 5 ,814
RTPM100% ,237 5 ,200 *
,961 5 ,814
alkohol70% ,237 5 ,200 *
,961 5 ,814
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.
Koloni_E.coli Based on Mean 1,934 7 32 ,097
Based on Median 1,300 7 32 ,282
Based on Median and with 1,300 7 16,198 ,311
adjusted df
Based on trimmed mean 1,899 7 32 ,103

ANOVA
Koloni_E.coli
Sum of Squares df Mean Square F Sig.
Between Groups 29589,900 7 4227,129 493,679 ,000
Within Groups 274,000 32 8,563
Total 29863,900 39

Koloni_E.coli
Duncan a

Subset for alpha = 0.05

Perlakuan_E.coli N 1 2 3 4 5 6
alkohol70% 5 A 25,60
RTPM100% 5 A 26,60
RT75%+PM100% 5 B 40,60
RT50%+PM100% 5 C 51,20
RT 100% 5 D 75,60
PM 100% 5 E 84,40
RT 75% 5 F 92,40
RT 50% 5 G 96,00
Sig. ,593 1,000 1,000 1,000 1,000 ,061
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.
 D. Pseudomonas aeruginosa
No Perlakuan Jumlah bakteri Pseudomonas aeruginosa
Standar
I II III IV V Rerata deviasi
1 A (50%) 108 91 101 105 95 100,00 + 7,00
2 Rumput Teki B (75%) 98 93 99 91 102 96,60 + 4,50
3 C (100%) 77 83 79 77 83 79,80 + 3,03
4 Putri Malu D (100%) 87 96 85 81 97 89,20 + 7,53
5 A + D 60 65 63 64 60 62,40 + 2,30
6 Kombinasi B + D 56 57 52 58 52 55,00 + 2,82
7 C + D 45 42 44 43 44 43,60 + 1,14
8 Positif Alkohol 70% 35 33 34 32 32 33,20 + 1,30

Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Perlakuan_P.aeruginosa Statistic df Sig. Statistic df Sig.
Koloni_P.aeruginosa RT50%_P.aeruginosa .162 5 .200 *
.959 5 .803
RT75%_P.aeruginosa .222 5 .200 *
.945 5 .701
RT100%_P.aeruginosa .254 5 .200 *
.803 5 .086
PM100%_P.aeruginosa .234 5 .200* .897 5 .396
RT50% + .251 5 .200* .868 5 .257
PM100%_P.aeruginosa
RT75% + .256 5 .200* .843 5 .174
PM100%_P.aeruginosa
RT100% + .237 5 .200* .961 5 .814
PM100%_P.aeruginosa
Alkohol 70%_P.aeruginosa .221 5 .200* .902 5 .421
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.
Koloni_P.aeruginosa Based on Mean 6.318 7 32 .000
Based on Median 2.598 7 32 .031
Based on Median and with 2.598 7 15.635 .055
adjusted df
Based on trimmed mean 6.275 7 32 .000
 ANOVA
Koloni_P.aeruginosa
Sum of Squares df Mean Square F Sig.
Between Groups 22030.975 7 3147.282 174.849 .000
Within Groups 576.000 32 18.000
Total 22606.975 39

Koloni_P.aeruginosa
Duncana
Subset for alpha = 0.05
Perlakuan_P.aeruginosa N 1 2 3 4 5 6 7
Alkohol 70%_P.aeruginosa 5 A 33.20
RT100% + 5 43.60
A
PM100%_P.aeruginosa
RT75% + 5 B55.00
PM100%_P.aeruginosa
RT50% + 5 C 62.40
PM100%_P.aeruginosa
RT100%_P.aeruginosa 5 D 79.80
PM100%_P.aeruginosa 5 F 89.20
RT75%_P.aeruginosa 5 G 96.60
RT50%_P.aeruginosa 5 G 100.00
Sig. 1.000 1.000 1.000 1.000 1.000 1.000 .214
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5.000.

Tolong yang P aeruginosa bisa dibuat setara Ealk spt E.coli agar memudahkan pembahasannya
dan tidak bertolok belakang dengan teori karena P.aeruginosa sbg flora normal ya
 E. Salmonella thyphi
No Perlakuan Jumlah bakteri Salmonella typhi Standar
Deviasi
I II III IV V Rerata
1 A (50%) 111 125 116 119 115 117,20 ±5.21
Rumput Teki
2 B (75%) 113 120 107 114 111 113,00 ±4.74
3 C (100%) 101 92 94 91 100 95,60 ±4.61
4 Putri Malu D (100%) 99 109 108 101 110 105,40 ±5.02
5 A + D 80 72 84 83 75 78,80 ±5.16
Kombinasi
6 B + D 72 67 70 68 71 69,60 ±2.07
7 C + D 55 50 52 53 51 52,50 ±1.92
8 Positif Alkohol 70% 36 35 34 32 33 34,00 ±1.58

Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Perlakuan_S.typhi Statistic df Sig. Statistic df Sig.
Koloni_S.typhi RT 50% ,191 5 ,200 *
,969 5 ,872
RT75% ,217 5 ,200 *
,974 5 ,902
RT100% ,236 5 ,200 *
,863 5 ,241
PM100% ,297 5 ,170 ,844 5 ,176
RT50%+PM100% ,192 5 ,200 *
,919 5 ,523
RT75%+PM100% ,180 5 ,200* ,952 5 ,754
RTPM100% ,141 5 ,200 *
,979 5 ,928
alkohol70% ,136 5 ,200 *
,987 5 ,967
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.
Koloni_S.typhi Based on Mean 2,393 7 32 ,044
Based on Median ,964 7 32 ,473
Based on Median and with ,964 7 20,935 ,481
adjusted df
Based on trimmed mean 2,319 7 32 ,049
 ANOVA
Koloni_S.typhi
Sum of Squares df Mean Square F Sig.
Between Groups 31382,975 7 4483,282 268,661 ,000
Within Groups 534,000 32 16,688
Total 31916,975 39

Koloni_S.typhi
Duncan a

Subset for alpha = 0.05

Perlakuan_S.typhi N 1 2 3 4 5 6 7
alkohol70% 5 A 34,00
RTPM100% 5 B 52,20
RT75%+PM100% 5 C 69,60
RT50%+PM100% 5 D 78,80
RT100% 5 E 95,60
PM100% 5 F 105,40
RT75% 5 G 113,00
RT 50% 5 G 117,20
Sig. 1,000 1,000 1,000 1,000 1,000 1,000 ,114
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 5,000.

CONTOH TABEL DAN GAMBAR

 S.epidermidis S.aureus E.coli P.aeruginosa S.typhi S.dysentriae

65.6
Average number of colonies (CFU/ml)

57.8
56.4

56.4

53.6

52.6
51.4
50.6

49.8
46.4

40.6
50,6

32.8

32.4
32.2
32.2

31.8
30.2
33

32
28.2

27.6
29

25.2
A. b 5 0 % +C . o 1 0 0 % A. b 7 5 % +C . o 1 0 0 % A. b 1 0 0 % +C . o 1 0 0 % Al c o h o l 7 0 %

Combination Treatment of A.blimbi and C.odorata Infusion against Test Bacteria

Figure 1. Combination Treatment of A.bilimbi and Cananga odorata Infusion Against Staphylococcus aureus,
Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi
and Shigella dysentriae

Figure 1. Combination activity of A. bilimbi fruit and C.odorata flowers against average number colonies of
Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Salmonella typhi, and Shigella dysentriae

Perlakuan Rerata Diameter Zona Hambat (mm)

S.aureus S.epidermidi E.coli S.typhi S.dysentriae
s
A.bilimbi 50% 12,64±0,18a 12,88±0,06a 9,94±0,03a 9,73±0,10a 9,73±0,10a
A.bilimbi 75% 10,51±0,16b 10,96±0,07b 7,88±0,03b 7,76±0,15b 7,76±0,15b
A.bilimbi 100% 12,05±0,10c 12,57±0,35c 9,47±0,09c 9,19±0,07c 9,19±0,07c
C.odorata 100% 13.40±0,15d 13,62±0,10d 11,64±0,06d 10,57±0,09d 10,57±0,09d

Ab.50%+Co.100% 13,87±0,05e 13,99±0,15e 11,65±0,14e 10,66±0,06e 10,66±0,06e

15,48±0,03f Concentration
15,78±0,09f 13,41±0,14f 12,26±0,07f 12,26±0,07f
AB.75%+Co.100%

AB1.00%+Co.100% 17,36±0,05g 17,57±0,35g 14,48±0,03g 14,12±0,04g 14,12±0,04g

Alkohol 70% 17,41±0,03g 17,48±0,03g 14,32±0,03g 14,18±0,04g 14,18±0,04g
Ab = A.bilimbi; Co = C. odorata
a, b, c, d, e,f, g, h, i, j, k, l, m, n superscript in the same column shows no significant difference (P>0.05)

Table 1. Results of Observation Coefficien Phenol Test of 5% Phenol against Some Test Bacteria
Combination treatmen of phenol 5% of test bacteria
Dilutions of each Average length of contact and repetition per unit time (minutes)
treatment S.aurues S.epidermidis E.coli Pa,eruginosa S.typhi
 5’ 10’ 15’ 5’ 10’ 15’ 5’ 10’ 15’ 5’ 10’ 15’ 5’ 10’ 15’

1:20 - - - - - - - - - - - - - - -

1:30 - - - - - - - - - - - - - - -

1:40 - - - - - - - - - - - - - - -

1:50 - - - - - - - - - - - - - - -

1:60 - - - - - - - - - - - - - - -

1:70 - - - - - - - - - - - - - - -

1:80 +3 - - +3 - - - - - - - -

1:90 +3 +3 +3 +3 +3 +3 +3 - -

1:100 +3 +3 +3 +3 +3 +3 +3 +3 -

1:110 +3 +3 +3 +3 +3 +3 +3 +3 +3

1:150 +3 +3 +3 +3 +3 +3 +3 +3 +3

1:200 +3 +3 +3 +3 +3 +3 +3 +3 +3

1:250 +3 +3 +3 +3 +3 +3 +3 +3 +3

Table 2. Results of Observation Coefficien Phenol Test of betel-basil leaf Combination Infusion of against some
test bacteria

Combination treatmen of betel-basil leaf infusion of test bacteria

Dilutions of each Average length of contact and repetition per unit time (minutes)
treatment S.aurues S.epidermidis E.coli Pa,eruginosa S.typhi

5’ 10’ 15’ 5’ 10’ 15’ 5’ 10’ 15’ 5’ 10’ 15’ 5’ 10’ 15’
1:20 - - - - - - - - - - - - - - -
1:30 - - - - - - - - - - - - - - -
1:40 - - - - - - - - - - - - - - -
1:50 - - - - - - - - - - - - - - -
1:60 - - - - - - - - - - - - - - -
1:70 - - - - - - - - - - - - - - -
1:80 - - - - - -
1:90
1:100
1:110
1:150
1:200
1:250
Information:
+ = There is growth of bacteria (cloudy medium)
- = No growth of bacteria (clear medium)
Table 4. Average Phenol Coefficient of Combination Infusion of Piper battle Linn and Ocimum sanctum Linn
Against SomeTest Bacteria

Phenol Coefficient Value

Treatments S.aureus S.epidermidi E.coli P.aeruginos S.typhi
s a
Betel+basil leaf infusion 1,00±0,01 1,02±0,00 0.98±0,01 0.96±0,01 0.87±0,07
Fennol 5% 1,00±0,00 1,00±0,00 1,00±0,00 1,00±0,00 1,00±0,00