Nama : Helmalia Shintawati

NIM : 19040053

Kelas : 19B

Kelompok/Shift : 02/01 (CUB)

PRAKTIKUM BIOFARMASETIKA

PERHITUNGAN “ABSORBSI OBAT PER ORAL SECARA IN VITRO”

Data kurva baku asam salisilat dalam NaCl 0,9%

Kadar (ppm) Absorbansi

5 0,115
10 0,222
15 0,337
20 0,440
30 0,645

Persamaan regresi linier  y = 0,021232432 x + 0,012081081

A = 0,012081081

B = 0,021232432

r = 0,999745871

“PERHITUNGAN CUB”

Perhitungan C (bpi)

CUB:

Menit ke-5

Y = 0,021232432 x + 0,012081081

0,835 = 0,021232432 x + 0,012081081

0,835-0,012081081 = 0,021232432 x

0,822918919 = 0,021232432 x
X = 38,75763827

X = 38,758 ppm

Menit ke-10

Y = 0,021232432 x + 0,012081081

0,350 = 0,021232432 x + 0,012081081

0,350-0,012081081 = 0,021232432 x

0,337918919 = 0,021232432 x

X = 15,91522436

X = 15,915 ppm

Menit ke-20

Y = 0,021232432 x + 0,012081081

0,195 = 0,021232432 x + 0,012081081

0,195-0,012081081 = 0,021232432 x

0,182918919 = 0,021232432 x

X = 8,615071462

X = 8,615 ppm

Menit ke-30

Y = 0,021232432 x + 0,012081081

0,243 = 0,021232432 x + 0,012081081

0,243-0,012081081 = 0,021232432 x

0,230918919 = 0,021232432 x

X = 10,87576397

X = 10,876 ppm

CLB:

Menit ke-5

Y = 0,021232432 x + 0,012081081
0,650 = 0,021232432 x + 0,012081081

0,650-0,012081081 = 0,021232432 x

0,637918919 = 0,021232432 x

X = 30,04455255

X = 30,045 ppm

Menit ke-10

Y = 0,021232432 x + 0,012081081

0,436 = 0,021232432 x + 0,012081081

0,436-0,012081081 = 0,021232432 x

0,423918919 = 0,021232432 x

X = 19,96563178

X = 19,966 ppm

Menit ke-20

Y = 0,021232432 x + 0,012081081

0,301 = 0,021232432 x + 0,012081081

0,301-0,012081081 = 0,021232432 x

0,288918919 = 0,021232432 x

X = 13,60743409

X = 13,607 ppm

Menit ke-30

Y = 0,021232432 x + 0,012081081

0,390 = 0,021232432 x + 0,012081081

0,390-0,012081081 = 0,021232432 x

0,377918919 = 0,021232432 x

X = 17,79913479

X = 17,799 ppm
Perhitungan Qb’

CUB:

Qb’ = C x Cairan volume serosal

Diketahui  cairan serosal = 3 mL

Menit ke-5:

Qb’ = 38,758 x 3 ml

= 116,274

Menit ke-10:

Qb’ = 15,915 x 3 ml

= 47,745

Menit ke-20:

Qb’ = 8,615 x 3 ml

= 25,845

Menit ke-30:

Qb’ = 10,876 x 3 ml

= 32,628

CLB:

Menit ke-5:

Qb’ = 30,045 x 3 ml

= 90,135

Menit ke-10:

Qb’ = 19,966 x 3 ml

= 59,898

Menit ke-20:

Qb’ = 13,607 x 3 ml

= 40,821
Menit ke-30:

Qb’ = 17,799 x 3 ml

= 53,397

Perhitungan Fk

(CUB):

Fk = C x Volume yang diambil

Diketahui  Volume yang diambil = 1,5 ml

Menit ke-5:

Qb’ = 38,758 x 1,5 ml

= 58,137

Menit ke-10:

Qb’ = 15,915 x 1,5 ml

= 23,8725

Menit ke-20:

Qb’ = 8,615 x 1,5 ml

= 12,9225

Menit ke-30:

Qb’ = 10,876 x 1,5 ml

= 16,314

CLB:

Menit ke-5:

Qb’ = 30,045 x 1,5 ml

= 45,0675

Menit ke-10:

Qb’ = 19,966 x 1,5 ml

= 29,949
Menit ke-20:

Qb’ = 13,607 x 1,5 ml

= 20,4105

Menit ke-30:

Qb’ = 17,799 x 1,5 ml

= 26,6985

Perhitungan Qb

CUB:

Qb = Qb’ + Fk

Menit ke-5:

Qb = 116,274 + 58,137

= 174,411

Menit ke-10:

Qb = 47,745 + 23,8725

= 71,6175

Menit ke-20:

Qb = 25,845 + 12,9225

= 38,7675

Menit ke-30:

Qb = 32,628 + 16,314

= 48,942

CLB:

Menit ke-5:

Qb = 90,135 + 45,0675

= 135,2025
Menit ke-10:

Qb = 59,898 + 29,949

= 89,847

Menit ke-20:

Qb = 40,821 + 20,4105

= 61,2315

Menit ke-30:

Qb = 53,397 + 26,6985

= 80,0955

Tabel. Hasil percobaan absorpsi asam salisilat per oral secara in vitro

Qb’ Fk Qb
Abs/ Y C (bpi)/ X
Menit ( μg ¿ ( μg ¿ ( μg ¿
ke
CUB CLB CUB CLB CUB CLB CUB CLB CUB CLB

5 0,835 0,650 38,758 30,045 116,274 90,135 58,137 45,0675 174,411 135,2025

10 0,350 0,436 15,915 19,966 47,745 59,898 23,8725 29,949 71,6175 89,847

20 0,195 0,301 8,615 13,607 25,845 40,821 12,9225 20,4105 38,7675 61,2315

30 0,243 0,390 10,876 17,799 32,628 53,397 16,314 26,6985 48,942 80,0955

PARAMETER ABSORPSI

Persamaaan regresi linier (t vs. Qb)

CUB  Y= -4,315667797 x + 153,5641017 ; r = -0,769417118

CLB  Y= -2,038347458 x + 124,7172712 ; r = -0,719586061

Perhitungan Pm

b
Pm =
Konsentrasi asam salisilat dalammukosal

Diketahui  nilai konsentrasi asam salisilat dalam mukosal = 5000 ppm

CUB:

−4,315667797
Pm =
5000 ppm

= -0,000863134

CLB:

−2,038347458
Pm =
5000 ppm

= -0,000407669

Perhitungan Lag time

Y=0

CUB:

Y = -4,315667797 x + 153,5641017

0 = -4,315667797 x + 153,5641017

0-153,5641017 = -4,315667797 x

-153,5641017 = -4,315667797 x

X = 35,58292921

X = 35,583

CLB:

Y = -2,038347458 x + 124,7172712

0 = -2,038347458 x + 124,7172712

0-124,7172712 = -2,038347458 x

-124,7172712 = -2,038347458 x

X = 61,18548175

X = 61,185
Tabel. Rekap hasil perhitungan parameter absopsi dari percobaan

Parameter Pada kondisi percobaan

absorpsi CUB CLB
K -4,315667797 -2,038347458
Pm -0,000863134 -0,000407669
Lag time 35,583 61,185