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  • GC Ansed

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    Ruliana Anu
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    This document summarizes the selectivity, linearity, accuracy, and precision of a fluorescence analysis method. It determines that the method has a linear response between 10-50 ppm with an R value of 0.9828. Recovery studies at 80%, 100%, and 120% of the standard concentration showed average recoveries between 96.7-90.2% with precisions below 0.65%. The limits of detection and quantification were determined to be 34.3 ppm and 10.3 ppm, respectively.

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    This document summarizes the selectivity, linearity, accuracy, and precision of a fluorescence analysis method. It determines that the method has a linear response between 10-50 ppm with an R value of 0.9828. Recovery studies at 80%, 100%, and 120% of the standard concentration showed average recoveries between 96.7-90.2% with precisions below 0.65%. The limits of detection and quantification were determined to be 34.3 ppm and 10.3 ppm, respectively.

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    8 views3 pages

    GC Ansed

    Uploaded by

    Ruliana Anu
    This document summarizes the selectivity, linearity, accuracy, and precision of a fluorescence analysis method. It determines that the method has a linear response between 10-50 ppm with an R value of 0.9828. Recovery studies at 80%, 100%, and 120% of the standard concentration showed average recoveries between 96.7-90.2% with precisions below 0.65%. The limits of detection and quantification were determined to be 34.3 ppm and 10.3 ppm, respectively.

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    of 3

    Selektivitas

    Baku induk flu = 30 ppm


    Baku induk matriks = 9,3 ppm

    Linearitas
    BA
    Kons LA
    10 177
    20 200
    30 320
    40 444
    50 550
    a = 41,2
    b = 9,9
    r = 0,9828

    Akurasi dan presisi


    BA 80%
    Rep Flu
    1 0,24 ml
    C teoritis = x 1000=24 ppm
    10 ml
    C sesungguhnya = 272χ = 23,3131ppm
    23,3131
    %recovery = x 100 %=97,1379 %
    24
    2 0,24 ml
    C teoritis = x 1000=24 ppm
    10 ml
    C sesungguhnya = 270χ = 23,1111ppm
    23 ,111 1
    %recovery = x 100 %=96,2962%
    24
    3 0,24 ml
    C teoritis = x 1000=24 ppm
    10 ml
    C sesungguhnya = 271χ = 23,2121ppm
    23 ,2 1 21
    %recovery = x 100 %=96,7170 %
    24
    SD
    %KV = x 100 %
    rata−rata kadar
    0,4208
    %KV = x 100 %=0,4351%
    97,7170

    100%
    Re Flu
    p
    1 0 ,3 ml
    C teoritis = x 1000=30 ppm
    10 ml
    C sesungguhnya = 309χ = 27,0505ppm
    27,0505
    %recovery = x 1 0 0 %=90,1683 %
    30
    2 0,3 ml
    C teoritis = x 1000=30 ppm
    10 ml
    C sesungguhnya = 310χ = 27,1515ppm
    27,1515
    %recovery = x 100 %=90,505 %
    30
    3 0,3 ml
    C teoritis = x 1000=30 ppm
    10 ml
    C sesungguhnya = 308χ = 26,9494ppm
    26,9494
    %recovery = x 100 %=89,8313 %
    30
    SD
    %KV = x 100 %
    rata−rata kadar
    0,3368
    %KV = x 100 %=0,3735 %
    90,1682
    120%
    Re Flu
    p
    1 0,3 6 ml
    C teoritis = x 1000=36 ppm
    10 ml
    C sesungguhnya = 348χ = 30,9898ppm
    30,9898
    %recovery = x 100 %=86,0827 %
    36
    2 0,36 ml
    C teoritis = x 1000=36 ppm
    10 ml
    C sesungguhnya = 346χ = 30,7878ppm
    30,7878
    %recovery = x 100 %=8 5,5216
    36
    3 0,36 ml
    C teoritis = x 1000=36 ppm
    10 ml
    C sesungguhnya = 350χ = 31,1919ppm
    31,1919
    %recovery = x 100 %=86,6441 %
    36
    SD
    %KV = x 100 %
    rata−rata kadar
    0,5612
    %KV = x 100 %=0,6519%
    86,0828
    LOD dan LOQ
    Konsentrasi Flu Luas Area (y) Luas area (y – ŷ)2
    (ppm) berdasarkan
    persamaan
    regresi
    (ŷ)
    10 177 140,2 1354,24
    20 200 239,2 1536,64
    30 320 338,2 331,24
    40 444 437,2 46,24
    50 550 536,2 190,44
    Jumlah 3458,8
    a = 41,2
    b = 9,9
    r = 0,9828
    y= 41,2 + 9,9x

    √
    2
    Sy/x = Ʃ ( y− ŷ )
    n−2

    =
    √ 3458,8
    5−2
    = 33,9548

    LOD :

    SB =
    √ 10 Sy /x
    yb
    =
    √
    10 x 33,9548
    9,9
    =34,2977

    LOQ :

    LOQ =
    √ 3 Sy / x
    yb
    =
    √
    3 x 33,9548
    9,9
    =10,2893

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