Lampiran 7.

Uji Kesesuaian Sistem KCKT

No. RT (menit) Area N

1. 1,280 21920786 1648,7414
2. 1,267 21770152 1612,7978
3. 1,259 21319063 1611,2474
4. 1,275 21528612 1612,4067
5. 1,271 21451407 1618,1260
6. 1,269 21550109 1595,8830
Rata-rata 1,270 21590021,5 1616,5337
SD 0,007 219020 17,4657
%RSD 0,57 1,01 1,08

Fase gerak = asetonitril : dapar fosfat pH 4 (70:30)

Laju alir= 1,0 mL/menit
Detektor = PDA
Kolom = SunfireTM C18

Penentuan Panjang Gelombang Maksimum Levofloksasin

1. Pembuatan baku levofloksasin 50 ppm
Kertas + zat = 0,5788 g
Kertas + sisa = 0,5207 g
zat = 0,0581 g
= 58,1 mg/100,0 mL
= 581 ppm pengenceran 10 x
V1 x C1 = V2 x C2
V1 x 500 ppm = 100,0 mL x 50 ppm
V1 = 10,0 mL ad 100,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
10,0 x 581 ppm = 100,0 mL x C2
C2 = 58,1 ppm

2. Karena tidak diketahui absortivity value dari levofloksasin maka dibuat

orientasi deret baku.
a. 2 ppm
V1 x C1 = V2 x C2
V1 x 50 ppm = 25,0 mL x 2 ppm
V1 = 1,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 58,1 ppm = 25,0 mL x C2
C2 = 2,32 ppm

b. 4 ppm
V1 x C1 = V2 x C2
V1 x 50 ppm = 25,0 mL x 4 ppm
V1 = 2,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
 2,0 x 58,1 ppm = 25,0 mL x C2
C2 = 4,64 ppm
c. 6 ppm
V1 x C1 = V2 x C2
V1 x 50 ppm = 25,0 mL x 6 ppm
V1 = 3,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 58,1 ppm = 25,0 mL x C2
C2 = 6,97 ppm
d. 8 ppm
V1 x C1 = V2 x C2
V1 x 50 ppm = 25,0 mL x 8 ppm
V1 = 4,0 mL ad 25,0 ml
Koreksi kadar
V1 x C1 = V2 x C2
4,0 x 58,1 ppm = 25,0 mL x C2
C2 = 9,29 ppm
e. 10 ppm
V1 x C1 = V2 x C2
V1 x 50 ppm = 50,0 mL x 10 ppm
V1 = 10,0 mL ad 50,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
10,0 x 58,1 ppm = 50,0 mL x C2
C2 = 11,62 ppm

Konsentrasi Absorbansi
2,32 ppm 0,2014 r= 0,9906
4,64 ppm 0,3496
6,97 ppm 0,6377
9,29 ppm 0,7621
11,62 ppm 0,9147

Pencarian panjang gelombang maksimum menggunakan baku tengah 6,97 ppm

didapatkan max = 295,6 dengan absorbansi 0,669
3. Perhitungan nilai absortivity valuedan ekstingsi molar
a. Konsentrasi 2,32 ppm
1%
A = A 1 cm Error: Reference source not foundx b x C(%)
0,2014 = Error: Reference source not found x 1 x 2,32 x 10-4
A 1%
1 cm = 868,1
BM
= Error: Reference source not found x
10
361,4
= 868,1 x
10
= 31373,13 / M.cm
b. Konsentrasi 4,64 ppm
1%
A = A 1 cm x b x C(%)

0,3496 = A 1%
1 cm x 1 x 4,64 x 10
-4

1%
A 1 cm = 753,4

= A 1%
1 cm x Error: Reference source not found

361,4
= 753,4 x
10
` = 27227,87 / M.cm

c. Konsentrasi 6,97 ppm

1%
A = A 1 cm x b x C(%)

0,6377 = A 1%
1 cm x 1 x 6,97 x 10
-4

1%
A 1 cm = 914,9
 = Error: Reference source not found x Error: Reference source not
found
= 914,9 x Error: Reference source not found
= 33064,48 / M.cm
d. Konsentrasi 9,29 ppm
A = Error: Reference source not found x b x C(%)
0,7621 = Error: Reference source not found x 1 x 9,29 x 10-4
Error: Reference source not found = 820,3
BM
= Error: Reference source not found x
10
= 820,3 x Error: Reference source not found
= 29645,64 / M.cm
e. Konsentrasi 11,62 ppm
A = Error: Reference source not found x b x C(%)
0,9147 = Error: Reference source not found x 1 x 11,62 x 10-4
Error: Reference source not found = 787,2
= Error: Reference source not found x Error: Reference source not
found
= 787,2 x Error: Reference source not found
= 28449,40 / M.cm
Rata-rata nilai = 29952,104 / M.cm
 Uji Selektivitas

Penimbangan baku levofloksasin 500 ppm

Kertas + zat = 0,5857 g
Kertas + sisa = 0,5359 g
zat = 0,049,8 g
= 49,8 mg/100,0 mL
= 498 ppm
Pelarut = asetonitril : dapar fosfat pH 4 (70:30)
Eluen = asetonitril : dapar fosfat pH 4 (70:30)
Matriks yang digunakan
R/ Levofloksasin
Sodium bikarbonat
HPMC
Asam sitrat
PVP
Magnesium stearat
Talkum
(Tej dkk, 2013)
 Uji Akurasi

1. Pembuatan larutan baku induk 500 ppm

Kertas + zat = 0,5857 g
Kertas + sisa = 0,5370 g
zat = 0,0487 g
= 48,7 mg/100,0 mL
= 487 ppm
2. Pengenceran baku
a. Baku 50 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 487 ppm = 10,0 mL x C2
C2 = 48,7 ppm
b. Baku 100 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 487 ppm = 25,0 mL x C2
C2 = 97,4 ppm
c. Baku 150 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
 3,0 x 487 ppm = 10,0 mL x C2
C2 = 146,1 ppm

3. Perhitungan sampel
% rata-rata %
Konsentrasi area kadar terukur SD
recovery recovery RSD
2830020 42,6545 ppm 101,56%
50 ppm 2831199 42,6723 ppm 99,94% 100,50% 0,9185 0,91%
2879723 43,4037 ppm 100,00%
6785686 107,1515 ppm 100,14%
100 ppm 6829198 107,8386 ppm 99,85% 100,38% 0,6768 0,67%
6853385 108,2205 ppm 101,14%
8983772 142,6562 ppm 100,11%
150 ppm 8877483 140,9684 ppm 100,62% 100,44% 0,2862 0,28%
8742374 138,8229 ppm 100,59%

a. Sampel 50 ppm
Replikasi 1
Kertas + zat = 0,5757 g
Kertas + sisa = 0,5287 g
zat = 0,0420 g
= 42 mg/100,0 mL
= 420 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 420 ppm = 10,0 mL x C2
C2 = 42 ppm
area sampel
Kadar terukur = Error: Reference source not foundError:
area baku
Reference source not found x konsentrasi baku
 2830020
= Error: Reference source not foundx 48,7
3231120
ppm
= 42,6545 ppm

% Recovery = Error: Reference source not foundError: Reference

source not foundx 100%
42,6545 ppm
= Error: Reference source not found 42 ppm
Error:

Reference source not foundx 100%

= 101,56%
Replikasi 2
Kertas + zat = 0,5874 g
Kertas + sisa = 0,5447 g
zat = 0,0427 g
= 42,7 mg/100,0 mL
= 427 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 427 ppm = 10,0 mL x C2
C2 = 42,7 ppm

Kadar terukur =Error: Reference source not found x konsentrasi baku

2831199
= x 48,7 ppm
3231120
= 42,6723 ppm

% Recovery = Error: Reference source not foundx 100%

 42,6723 ppm
= Error: Reference source not found 42,7 ppm
x

100%
= 99,94%
Replikasi 3
Kertas + zat = 0,5597 g
Kertas + sisa = 0,5163 g
zat = 0,0434 g
= 43,4 mg/100,0 mL
= 434 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 434 ppm = 10,0 mL x C2
C2 = 43,4 ppm
Kadar terukur =Error: Reference source not found x konsentrasi baku
2879723
= Error: Reference source not found x 48,7
3231120
ppm
= 43,4037 ppm
% Recovery = Error: Reference source not foundx 100%
43,4037 ppm
= 143,4 ppm x 100%

= 100,00%
b. Sampel 100 ppm
Replikasi 1
Kertas + zat = 0,5671 g
Kertas + sisa = 0,5736 g
zat = 0,0535 g
 = 53,5 mg/100,0 mL
= 535 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 535 ppm = 25,0 mL x C2
C2 = 107 ppm
Kadar terukur =Error: Reference source not found x konsentrasi baku
6785686
= x 97,4 ppm
6168144
= 107,1515 ppm
% Recovery = Error: Reference source not foundx 100%
107,1515 ppm
= 107 ppm
x 100%

= 100,14%
Replikasi 2
Kertas + zat = 0,5488 g
Kertas + sisa = 0,4948 g
zat = 0,0540 g
= 54 mg/100,0 mL
= 540 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 540 ppm = 25,0 mL x C2
C2 = 108 ppm
Kadar terukur =Error: Reference source not found x konsentrasi baku
6829198
= x 97,4 ppm
6168144
= 107,8386 ppm
% Recovery = Error: Reference source not foundx 100%
107,8386 ppm
= 108 ppm
x 100%

= 99,85%
Replikasi 3
Kertas + zat = 0,5667 g
Kertas + sisa = 0,5132 g
zat = 0,0535 g
= 53,5 mg/100,0 mL
= 535 ppm

Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 535 ppm = 25,0 mL x C2
C2 = 107 ppm
Kadar terukur =Error: Reference source not found x konsentrasi baku
6853385
= x 97,4 ppm
6168144
= 107,2205 ppm
% Recovery = Error: Reference source not foundx 100%
108,2205 ppm
= 107 ppm
x 100%

= 101,14%
c. Sampel 150 ppm
Replikasi 1
Kertas + zat = 0,5368 g
Kertas + sisa = 0,4893 g
zat = 0,0475 g
= 47,5 mg/100,0 mL
= 475 ppm

Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL

Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 475 ppm = 10,0 mL x C2
C2 = 142,5 ppm
Kadar terukur =Error: Reference source not found x konsentrasi baku
8983772
= x 146,1 ppm
9200646
= 142,6562 ppm
% Recovery = Error: Reference source not foundx 100%
142,6562 ppm
= Error: Reference source not found 142,5 ppm x

100%
= 100,11%
Replikasi 2
Kertas + zat = 0,5791 g
Kertas + sisa = 0,5324 g
zat = 0,0467 g
= 46,7 mg/100,0 mL
= 467 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 467 ppm = 10,0 mL x C2
C2 = 140,1 ppm

Kadar terukur =Error: Reference source not found x konsentrasi baku

8977483
= x 146,1 ppm
9200646
= 140,9684 ppm

% Recovery = Error: Reference source not foundx 100%

140,9684 ppm
= 140,1 ppm
x 100%

= 100,62%

Replikasi 3
Kertas + zat = 0,5665 g
Kertas + sisa = 0,5205 g
zat = 0,0460 g
= 46 mg/100,0 mL
= 460 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL

Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 460 ppm = 10,0 mL x C2
C2 = 138 ppm

Kadar terukur =Error: Reference source not found x konsentrasi baku

8742374
= x 146,1 ppm
9200646
= 138,8229 ppm

kadar terukur
% Recovery = kadar sebenarnya Error: Reference source not foundx

100%
138,8229 ppm
= 138 ppm
x 100%

= 100,59%
 Uji Presisi Repeatability

1. Sampel konsentrasi 50 ppm

Replikasi 1
Kertas + zat = 0,5745 g
Kertas + sisa = 0,52851 g
zat = 0,0460 g
= 46 mg/100,0 mL
= 460 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 460 ppm = 10,0 mL x C2
C2 = 46 ppm
Replikasi 2
Kertas + zat = 0,5680 g
Kertas + sisa = 0,5202 g
zat = 0,0478 g
= 47,8 mg/100,0 mL
= 478 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 478 ppm = 10,0 mL x C2
C2 = 47,8 ppm
 Replikasi 3
Kertas + zat = 0,5711 g
Kertas + sisa = 0,5251 g
zat = 0,0460 g
= 46 mg/100,0 mL
= 460 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 50 ppm
V1 = 1,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 460 ppm = 10,0 mL x C2
C2 = 46 ppm
Replikasi RT (menit) Area Tailing factor N
1 1,277 2909312 1,5291 4436,3607
2 1,275 2958833 1,5282 4437,1771
3 1,277 2884164 1,53 4423,6234
rata-rata 1,276 2917436,5 1,5291 4432,387
SD 0,001 37991,9 0,0009 7,6005
% RSD 0,09 1,30 0,05 0,17

2. Sampel konsentrasi 100 ppm

Replikasi 1
Kertas + zat = 0,5725 g
Kertas + sisa = 0,5234 g
zat = 0,0491 g
= 49,1 mg/100,0 mL
= 491 ppm

Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 491 ppm = 25,0 mL x C2
C2 = 98,2 ppm
Replikasi 2
Kertas + zat = 0,5630 g
Kertas + sisa = 0,5144 g
zat = 0,0486 g
= 48,6 mg/100,0 mL
= 486 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 486 ppm = 25,0 mL x C2
C2 = 97,2 ppm
Replikasi 3
Kertas + zat = 0,5719 g
Kertas + sisa = 0,5223 g
zat = 0,0496 g
= 49,6 mg/100,0 mL
= 496 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 25,0 mL x 100 ppm
V1 = 5,0 mL ad 25,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
 5,0 x 496 ppm = 25,0 mL x C2
C2 = 99,2 ppm
Replikasi RT (menit) Area Tailing factor N
1 1,304 7387129 1,6564 3577,3037
2 1,301 7331282 1,6569 3600,1497
3 1,292 7526848 1,6568 3507,6879
rata-rata 1,299 7415086,4 1,6567 3561,7137
SD 0,007 100735,6 0,0002 48,162
% RSD 0,51 1,36 0,01 1,35

3. Sampel konsentrasi 150 ppm

Replikasi 1
Kertas + zat = 0,5458 g
Kertas + sisa = 0,4973 g
zat = 0,0485 g
= 48,5 mg/100,0 mL
= 485 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 485 ppm = 10,0 mL x C2
C2 = 145,5 ppm
Replikasi 2
Kertas + zat = 0,5540 g
Kertas + sisa = 0,5048 g
zat = 0,0492 g
= 49,2 mg/100,0 mL
= 492 ppm
 Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 492 ppm = 10,0 mL x C2
C2 = 147,6 ppm
Replikasi 3
Kertas + zat = 0,5672 g
Kertas + sisa = 0,5189 g
zat = 0,0483 g
= 48,3 mg/100,0 mL
= 483 ppm
Pengenceran
V1 x C1 = V2 x C2
V1 x 500 ppm = 10,0 mL x 150 ppm
V1 = 3,0 mL ad 10,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 483 ppm = 10,0 mL x C2
C2 = 144,9 ppm
Replikasi RT (menit) Area Tailing factor N
1 1,289 11053951 1,7351 3157,3376
2 1,287 11082014 1,7308 3159,1538
3 1,287 10965363 1,7292 3132,5493
rata-rata 1,288 11027776,2 1,7317 3149,6802
SD 0,001 58753,4 0,003 14,8635
% RSD 0,09 0,53 0,17 0,47

Uji Linieritas
1. Pembuatan larutan baku induk 1000 ppm
Kertas + zat = 0,5620 g
Kertas + sisa = 0,5104 g
zat = 0,0511 g
= 51,1 mg/50,0 mL
= 1022 ppm
2. Pembuatan deret baku
a. 20 ppm
V1 x C1 = V2 x C2
V1 x 1000 ppm = 50,0 mL x 20 ppm
V1 = 1,0 mL ad 50,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 1022 ppm = 50,0 mL x C2
C2 = 20,44 ppm
b. 30 ppm
V1 x C1 = V2 x C2
V1 x 1000 ppm = 100,0 mL x 30 ppm
V1 = 3,0 mL ad 100,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 1022 ppm = 100,0 mL x C2
C2 = 30,66 ppm
c. 40 ppm
V1 x C1 = V2 x C2
V1 x 1000 ppm = 25,0 mL x 40 ppm
V1 = 1,0 mL ad 25,0 mL

Koreksi kadar
V1 x C1 = V2 x C2
 1,0 x 1022 ppm = 25,0 mL x C2
C2 = 40,88 ppm
d. 50 ppm
V1 x C1 = V2 x C2
V1 x 1000 ppm = 100,0 mL x 50 ppm
V1 = 5,0 mL ad 100,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
5,0 x 1022 ppm = 100,0 mL x C2
C2 = 51,1 ppm
e. 60 ppm
V1 x C1 = V2 x C2
V1 x 1000 ppm = 50,0 mL x 60 ppm
V1 = 3,0 mL ad 50,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 1022 ppm = 50,0 mL x C2
C2 = 61,32 ppm
Baku (ppm) Konsentrasi sebenarnya (ppm) Area
20 20,44 1185328
30 30,66 1743091
40 40,88 2367202
50 51,1 2962014
60 61,32 3454431
 Uji Sensitivitas (LOD-LOQ)

1. Pembuatan baku induk 500 ppm

Kertas + zat = 0,5765 g
Kertas + sisa = 0,5274 g
zat = 0,0491 g
= 49,1 mg/100,0 mL
= 491 ppm
2. Pembuatan deret baku
a. 2 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 250,0 mL x 2 ppm
V1 = 1,0 mL ad 250,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
1,0 x 491 ppm = 250,0 mL x C2
C2 = 1,964 ppm
b. 4 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 250,0 mL x 4 ppm
V1 = 2,0 mL ad 250,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
2,0 x 491 ppm = 250,0 mL x C2
C2 = 3,928 ppm
c. 6 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 250,0 mL x 6 ppm
V1 = 3,0 mL ad 250,0 mL
 Koreksi kadar
V1 x C1 = V2 x C2
3,0 x 491 ppm = 250,0 mL x C2
C2 = 5,892 ppm

d. 8 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 250,0 mL x 8 ppm
V1 = 4,0 mL ad 250,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
4,0 x 491 ppm = 250,0 mL x C2
C2 = 7,856 ppm
e. 10 ppm
V1 x C1 = V2 x C2
V1 x 500 ppm = 100,0 mL x 10 ppm
V1 = 2,0 mL ad 100,0 mL
Koreksi kadar
V1 x C1 = V2 x C2
2,0 x 491 ppm = 100,0 mL x C2
C2 = 9,82 ppm
Konsentrasi (x) Area (y) a = 10654,7
1,964 ppm 125304
b = 57194,7556
3,928 ppm 222020
5,892 ppm 359510 r = 0,9983
7,856 ppm 466861 y = 57194,7556x + 10654,7

9,820 ppm 564536

X2 Yhitung Y - Yhitung
3,8573 -3,928 15,4292 122985 2318,8 5376833,4
15,4292 -1,964 3,8573 235315,7 -13295,7 176775638,5
34,7157 5,892 0 0 347646,2 11863,8 140749750,4
61,7167 1,964 3,8573 459976,7 6884,3 47393586,4
96,4324 3,928 15,4292 572307,2 -7771,19 60391394
(Y-Yhitung)2 = 430687202,9

(Y - Yhitung) 2 430687202,9
Slope = Error: Reference source not found = Error:
n-2 5-2

Reference source not found = 11981,7528

3 x slope 3 x 11981,7528
LOD = = 57194,7556 = 0,62847 ppm
b
10 x slope 10 x 11981,7528
LOQ = = 57194,7556 = 2,0949 ppm
b