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  • Kfa Kelompok 3 Validasi

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    Febbio Yoghatama
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    This document summarizes the analysis of a group project in pharmaceutical chemistry. It outlines the group members, describes the process of creating a stock solution and calibration standards, establishes a calibration curve with absorption vs. concentration, determines the concentration of a sample, and validates the results with recovery percentages. The group created a stock solution, then calibration standards from 1-5 ppm and measured their absorbance. They established a linear calibration curve and used it to determine the sample concentration was 18.96 ppm. Validation at two levels showed recovery percentages ranging from 94-99% with an average of 96.23% and RSD of 1.81%.

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    KFA KELOMPOK 3 VALIDASI

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    This document summarizes the analysis of a group project in pharmaceutical chemistry. It outlines the group members, describes the process of creating a stock solution and calibration standards, establishes a calibration curve with absorption vs. concentration, determines the concentration of a sample, and validates the results with recovery percentages. The group created a stock solution, then calibration standards from 1-5 ppm and measured their absorbance. They established a linear calibration curve and used it to determine the sample concentration was 18.96 ppm. Validation at two levels showed recovery percentages ranging from 94-99% with an average of 96.23% and RSD of 1.81%.

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    10 views15 pages

    Kfa Kelompok 3 Validasi

    Uploaded by

    Febbio Yoghatama
    This document summarizes the analysis of a group project in pharmaceutical chemistry. It outlines the group members, describes the process of creating a stock solution and calibration standards, establishes a calibration curve with absorption vs. concentration, determines the concentration of a sample, and validates the results with recovery percentages. The group created a stock solution, then calibration standards from 1-5 ppm and measured their absorbance. They established a linear calibration curve and used it to determine the sample concentration was 18.96 ppm. Validation at two levels showed recovery percentages ranging from 94-99% with an average of 96.23% and RSD of 1.81%.

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    of 15

    Kelompok 3

    KIMIA FARMASI
    ANALISIS
    Anggota Kelompok
    UMI SAQDIYAH (201910410311029)
    TASRIPAH (201910410311030)
    BINTI ISLAMIYAH (201910410311031)
    AFIFAH SALSABILA C. (201910410311032)
    NADYA FARICHA P. (201910410311034)
    AFSARI SALSABELLA (201910410311035)
    NUR FADILAH (201910410311036)
    MAULIDATURRAHMAH (201910410311037)
    DWI AZZAHRA P. (201910410311038)
    AZIZ MUHAMMAD F. (201910410311039)
    HANIF YULIA (201910410311040)
    FEBBIO YOGHATAMA (201910410311041)
    Perhitungan Larutan Induk & Larutan Baku Induk

    LARUTAN INDUK
    Konsentrasi = ppm = mg/L
    = 260,1 mg / 100 ml
    =260,1 mg /0,1 L
    = 2601 ppm

    LARUTAN BAKU INDUK 1


    V1.N1=V2.N2
    5ml x 2601 ppm =50,0 ml x N2
    N2 = 260,1 ppm
    Perhitungan Konsentrasi Baku Kerja 1-5

    Baku Kerja 1
    V1.N1=V2.N2
    1.0 ml x 260.1 ppm=25.0 ml x N2
    Baku Kerja 4 N2 = 10.404 ppm
    V1.N1=V2.N2
    4.0 ml x 260.1 ppm = 25.0 ml x N2
    N2 = 41.616 ppm Baku Kerja 2
    V1.N1=V2.N2
    2.0ml x 260.1 ppm = 25.0 ml x N2
    Baku Kerja 5 N2= 20.808 ppm
    V1.N1=V2.N2
    5.0 ml x 260,1 ppm = 25.0 ml x N2
    N2= 52.02 ppm Baku Kerja 3
    V1.N1=V2.N2
    3.0 ml x 260.1 ppm = 25.0 ml x N2
    N2 = 31.212 ppm
    Persamaan
    Regresi
    Larutan Konsentrasi Absorban
    Baku Kerja 1 10,404 ppm 0,111
    Baku Kerja 2 20,808 ppm 0,225
    Baku Kerja 3 31,212 ppm 0,329
    Baku Kerja 4 41,616 ppm 0,451
    Baku Kerja 5 52,02 ppm 0,562

    a = -0,0028
    b = 0.0108
    r = 0.9998
    Kadar Sampel dan Validasi
    Larutan Konsentrasi Absorban
    Sampel ? 0,202

     
    Kadar Sampel
    y= bx + a
    0,202 = 0,0108x – 0,0028
    X=
    X = 18,96 ppm
    VALIDASI
    Larutan Konsentrasi Absorban
     
    VALIDASI 1A
    y=bx+a Validasi 1A ? 0,419
    0,419 = 0,0108x – 0,0028
    Validasi 1B ? 0,425
    X== 39.06 ppm
    Validasi 1C ? 0,416
    VALIDASI 1B
    y=bx+a
    0,425 = 0,0108x – 0,0028
    X== 39.61 ppm

    VALIDASI 1C
    y=bx+a
    0,416 = 0,0108x – 0,0028
    X== 38.78 ppm
    VALIDASI
    Larutan Konsentrasi Absorban
     
    VALIDASI 2A
    Validasi 2A ? 0,527
    y=bx+a
    0,527 = 0,0108x – 0,0028 Validasi 2B ? 0,519
    X== 49.06 ppm
    Validasi 2C ? 0,525

    VALIDASI 2B
    y=bx+a
    0,519 = 0,0108x – 0,0028
    X== 48.31 ppm

    VALIDASI 2C
    y=bx+a
    0,525 = 0,0108x – 0,0028
    X== 48.87 ppm
    No. LARUTAN Konsentrasi Absorban
    1. Baku Kerja 1 10,404 ppm 0,111
    2. Baku Kerja 2 20,808 ppm 0,225
    3. Baku Kerja 3 31,212 ppm 0,329
    4. Baku Kerja 4 41,616 ppm 0,451
    5. Baku Kerja 5 52,02 ppm 0,562
    6. Sampel 18,96 ppm 0,202
    7. Validasi 1A 39,06 ppm 0,419
    8. Validasi 1B 39,61 ppm 0,425
    9. Validasi 1C 38,78 ppm 0,416
    10. Validasi 2A 49,06 ppm 0,527
    11. Validasi 2B 48,31 ppm 0,519
    12. Validasi 2C 48,87 ppm 0,525
    % Recovery
     
    %Recovery=
    %R Validasi 1A=
    = 96,6 %
    %R Validasi 1B=
    = 99,24 %
    %R Validasi 1C=
    = 95,25 %
    % Recovery
     
    %Recovery=
    %R Validasi 2A=
    = 96,44 %
    %R Validasi 2B=
    = 94,03 %
    %R Validasi 2C=
    = 95,83 %
    Presisi
    Validasi % Recovery
    Validasi 1A 96,6 %
    Validasi 1B 99,24 %
    Validasi 1C 95.25 %
    Validasi 2A 96,44 %
    Validasi 2B 94,03 %
    Validasi 2C 95,83 %
    Rata-rata 96,23 %
    SD 1,74

     
    RSD =
    RSD =
    RSD =1,81 %
    TERIMA KASIH

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