PERHITUNGAN P3 SHIFT 1

VII. PENYELESAIAN MASALAH

1. Pembuatan plasma darah

Sampel darah (mL) TCA 10% (mL) Volume supernatant (mL)

1 1 1

2. Pembuatan larutan baku parasetamol

- Pembuatan larutan stok parasetamol 100 mg/L :
Parasetamol : ……10…….. mg
Fase gerak :………100……...mL

Konsentrasi Larutan stok parasetamol

Fase gerak (mL)
baku teofilin 100 mg/L (mL)
10 μg/ml 1 9
20 μg/ml 2 8
30 μg/ml 3 7
40 μg/ml 4 6
50 μg/ml 5 5

3. Pembuatan Sampel
Plasma : ……1……..mL
Aquadest :…..…6….mL
Larutan sampel parasetamol 100 ppm : .....1....mL
PENYELESAIAN MASALAH ARTIKEL
1. Pembuatan kurva baku
100 𝑚𝑔
100 ppm = 100 𝑚𝐿
100 𝑚𝑔 𝑥
= 100 𝑚𝐿 = 100 𝑚𝐿

x = 10 mg
➢ Pengenceran Larutan Baku
- 10 ppm
M1 x V1 = M2 x V2
100 ppm x V1 = 10 ppm x 10 mL
V1 = 1 mL
- 20 ppm
M1 x V1 = M2 x V2
100 ppm x V1 = 20 ppm x 10 mL
V1 = 2 mL
- 30 ppm
M1 x V1 = M2 x V2
100 ppm x V1 = 30 ppm x 10 mL
V1 = 3 mL
- 40 ppm
M1 x V1 = M2 x V2
100 ppm x V1 = 40 ppm x 10 mL
V1 = 4 mL
- 50 ppm
M1 x V1 = M2 x V2
100 ppm x V1 = 50 ppm x 10 mL
V1 = 5 mL

No. Seri kadar Luas area

1. 10 ppm 851437
2. 20 ppm 857692
3. 30 ppm 1658249
4. 40 ppm 8643080
5. 50 ppm 11075926

➢ Luas area sampel = 28093822

 2. Kurva baku parasetamol dan penetapan regresi linier

Kurva Baku Parasetamol

12000000
y = 282344x - 4E+06
10000000 R² = 0.8392

8000000
Luas Area

6000000

4000000

2000000

0
0 10 20 30 40 50 60
-2000000
Konsentrasi (ppm)

Regresi linier = 282343x + (-3853033)

Nilai R = 0,916

3. Penetapan kadar parasetamol pada sampel darah

VIII. Perhitungan
1. Kadar parasetamol
Y = 282343x + (-3853033)
28093822 = 282343x - 3853033
x = 113,149 µg/mL
2. Perhitungan Bobot Sampel
Bobot sampel = kadar sampel (x) × 100 mL
= 113,149 µg/mL x 100 mL
= 11.314,9 µg
= 11,314 mg
3. Peritungan Kadar Paracetamol terhadap Sampel
𝐵𝑜𝑏𝑜𝑡 𝑠𝑎𝑚𝑝𝑒𝑙
Kadar sampel = 𝐵𝑜𝑏𝑜𝑡 𝑦𝑎𝑛𝑔 𝑑𝑖𝑔𝑢𝑛𝑎𝑘𝑎𝑛 × 100%

11,314 𝑚𝑔
= x 100%
590 𝑚𝑔

= 1,917 % (b/b)
4. Perhitungan Kadar Paracetamol terhadap kadar di etiket
𝐵𝑜𝑏𝑜𝑡 𝑠𝑎𝑚𝑝𝑒𝑙
Kadar paracetamol terhadap etiket = 𝐵𝑜𝑏𝑜𝑡 𝑝𝑎𝑑𝑎 𝑒𝑡𝑖𝑘𝑒𝑡 × 100%

11,314 𝑚𝑔
= x 100%
500 𝑚𝑔

= 2,262 % (b/b)