Research Program 3: Photonics in Life Sciences

Project 9- Optical Tweezers

Coordinator- Carlos Lenz Cesar
Campinas branch
1) Optical Tweezers with Linear and Nonlinear Microspectroscopy and Microscopy.
2) Optical Tweezers for Red Blood Cells Rheoscopy

Project 10- Lasers application in dentistry and medicine

Coordinator- Denise Maria Zezell
São Paulo branch
1) Physical and Chemical Proprierties of Dental Tissue
2) Optical Diagnostic Methods
3) Low Intensity Lasers –research
4) Low Intensity Lasers clinical research
5) High Intensity Lasers in Restorative Dentistry and Periodontics
6) Lasers in Endodontics

Project 9- Optical Twizers

Coordinator- Carlos Lenz Cesar- UNICAMP-
Campinas branch

Optical Tweezers with Linear and Nonlinear Microspectroscopy and Microscopy.

We decided to add spectroscopy to the optical tweezer system to perform

spectroscopy at the same time we manipulate particles and living cells, allowing us to
observe chemical reactions and mechanical properties changes in real time. For this we
incorporated a home made confocal spectrometer to our optical tweezer setup and succeed
to observe Raman, Hyper Rayleigh (Second Harmonic) and Hyper Raman scattering, two-
photon absorption (TPA) excited photoluminescence and force spectroscopy. The hyper
Raman and Rayleigh term comes from the expansion of the second order susceptibility
± iω j t
χ ( 2 ) = χ ( 2 ) (0) + (∂χ ( 2 ) (0) ∂q j ) q j ± e of the polarization vector
r r r r rr
P( E ) = Po + χ (1) (q j ) : E + χ ( 2) (q j ) : EE + ... at the 2ω and 2ω ± ω j frequencies, and
their tensors are different from the Raman and Rayleigh ones, with opposite parity. In the
past hyper Raman has been observed only with giant picosecond pulses after averaging
times of hours. We observed it with seconds because our 80 MHz femtosecond Ti:sapphire
laser averages 107 more pulses than a tipical 10 Hz system used before. Hyper Rayleigh and
Raman are two photon processes and as such they are intrinsically confocal techniques with
the same advantage of the multiphoton confocal microscopy. With this system we have
been able to observe the Raman spectra of ZnSe and trapped polystirene sphere and red
blood cells (RBC) at 785 nm region, Figura 1, TPA excited luminescence at 500 nm region
and the hyper Rayleigh and Hyper Raman scattering at 400 nm of ZnSe, Figura 2. The TPA
excited luminescence spectra of a trapped DAPI stained latex microsphere clearly show the
“Morphology Dependent Resonances”, Figura 3., with good agreement with Mie
Scattering calculations for more than 15 peaks. At the same time these resonances increase
the light scattering they increase the optical force, therefore we decided to revisited 1977
Ashkin’s force spectroscopy experiment [PRL 38, 1351,1977]. By that time Ashkin used an
unstable levitation system to observe the optical forces at microspheres. We, instead, used a
stable single beam optical trap perturbed by a second tunable Ti:sapphire laser to perform
the force spectroscopy showed in Figura 4. Because we have two laser beams we can
position each one respect to the other individualy and observe the coupling to the
whispering gallery modes as a function of polarization and position. The three spectrum
showed correspond to the Ti:sapphire laser beam positioned at the edge of the microsphere
but with different polarization. As we can see for the (a) case only the TE modes are
excited, for (b) case only the TM modes and for the (c) we excited both modes. This is the
same spectrum we obtain when the sphere is illuminated by a plane wave as shown in
Figura 5. For this case the Ti:sapphire laser focus was positioned well above the Nd:Yag
focus to guarantee the ilumination field is like a plane wave.
Optical Tweezers for Red Blood Cells Rheoscopy

In this area the two important facts were Marcelo Brandão’s PhD thesis defense and the
use of double optical tweezer to understand red blood cells adhesion. Brandão’s thesis
included the results of the RBC elasticity measurements and comparison with normal
controls (HbAA) several diseases an conditons. The diseases and conditions studied were:
homozygous (HbSS) and heterozygous (HbAS) sickle cell anemia, hereditary spherocytosis
and iron deficiency anemia. These results showed that the laser optical tweezers technique
is able to detect differences in HbS red blood cells from subjects taking hydroxyurea, as
well as to differentiate red blood cells from normal controls and HbAS, sickle-cell trait,
indicating that this is a very sensitive method and can be applied for detection of drug-
response in sickle cell disease. The thesis also included the studies of storage of gamma
irradiated blood bags. The gamma irradiation is used to prevent transfusion associated
graft-versus-host disease in immunodeficient patients and this study aim was to understand
the hematological and biochemical changes induced by the irraditaion. The changes in
elasticity after 20 storage days was so high compared to non irradiated RBC, that we
suggested the rule to use irradiated blood bags up to 35 storage days to be reviewed. The
double tweezer was used to observe the adhesion of RBC’s. Figures 5.a, 5.b and 5.c show
a sequence of 6 RBC’s sticked together (rouleaux) under the forces of the two optical
tweezers. We observe that they slide one over the other very easily but they adhere very
strongly at the edges. The RBC’s adherence is an important and not well understood
phenomena in which electrostactic, RBC are negativelly charged, and van der waal’s
chemical forces play their roles. The ph and dielectric constant of the solution they are
embedded are important from the electrostatic point of view while membrane proteins and
antibodies represent the glue factor. For the first time we have a tool able to measure the
force as function of the displacement between two RBC’s and to check the existing models
and even developed a new one for the adherence force between cells and microorganisms.

Researchers in Project 09
Name
Carlos Lenz Cesar
Sara T. O. Saad
Maria Lourdes Barjas-Castro
Vagner de Castro
Vivaldo Moura Neto- UFRJ
Katsuhiro Ajito (visiting- NT&T)

Students PhD (D)

Master of
Sciences (M)
Adriana Fontes D
Marcelo Mendes Brandão D

Master of Science dissertation

Marcelo Mendes Brandão. PhD Thesis: "Análise e caracterização da deformidade de
eritrócitos em membranopatias", defense - May 2003, Medical College - UNICAMP.

Published articles:
A. Fontes, K. Ajito, A. M. De Paula, A. R. Neves, W. L. Moreira, L. C. Barbosa and C.
L. Cesar, “Raman, Hyper-Raman, Hyper-Rayleigh and Two-Photon Excited
Luminescence Microespectroscopy ina an Optical Tweezers System”, Microsc.
Microanal. 9 (suppl. 2) 164-165 (2003).

M. M. Brandão, A. Fontes, M. L. Barjas-Castro, L. C. Barbosa, F. F. Costa, C. L. Cesar

and S. T. O. Saad, “Optical Tweezers for Measuring red blood cell elasticity:
Application to the study of drug response in sickle cell disease”, Eur. J. Haematol. 70,
207-211 (2003).
Accepted Articles
M. M. Brandão, S. T. O. Saad, A. Fontes, C. L. Cesar, F. F. Costa, and M. L. Barjas-
Castro; “Elastic properties of stored red blood cell units from donors with sickle cell
trait”; accepted Vox Sanguinis – may/2003.

Photos and graphs:

CW Raman Spe ctra

trapped polystyrene
Intensity (a.u.

microsphere
(b)

ZnSe
trapped
red blood cell

(a) (c)

0 250 500 750 1000

-1
raman shift (cm )

Figura 1
 ZnSe: TPA PL, Hyper-Rayleigh/Raman
ZnSe TPA Luminescence
ZnSe Hyper Rayleigh
Intensity (a.u.)

ZnSe Hyper
Raman
Stokes

ZnSe Hyper
Raman
AntiStokes
(b)
(a)

360 410 460 510

wavelength (nm)

Figura 2

TPA photoluminescence in DAPI stained microsphere

Rfit = 2.72 µm Experimental

Nominal: 3 µm ± 10%
Intensity (a.u)

10.087 × 10 −11
n1 = 1.5683 +
λ
3.046 ×10 −11
n2 = 1.324 +
λ
Theoretical

438 448 458 468 478 488 498

w avelength (nm)

Figura 3.
 Optical force spectroscopy of 9 µm latex sphere

(b)
(c) (c)
Optical Force (a.u.)

(a)

(a)
(b)

710 720 730 740 750 760

wavelength (nm)

Figura 4.

Optical force spectroscopy of 9 µm latex sphere

optical force (a.u.)

720 730 740 750 760 770

wavelength (nm)

Figura 5
Figura 6- (5.a) RBC’s rouleaux (5.b) sliding on top of the other (5.c) edges strong adherence
 Project 10- Lasers application in dentistry and medicine
Coordinator- Denise Maria Zezell
São Paulo branch

Physical and Chemical Proprieties of dental hard tissue

Enamel surfaces irradiated with several wavelength and pulse widths are under
investigation in order to develop new processes for caries prevention using lasers. [Ca]/[P],
fluoride uptake, morphological and microbiological studies are the main analysis methods.
The infrared spectroscopy results show that the erbium laser (2,94µm) changes the structure
and composition of the organic matrix, OH- radical and the water composition in the
irradiated dentin. The crystalline structure of irradiated enamel with holmium laser (2.065
µm) indicates the existence of a mixture of two crystalline phases: hydroxyapatite and
tetracalcium phosphate ( Figura 7), that is more resistant to the acid produced by cariogenic
microorganisms. The dentin discoloration after thermal treatment can be due to the non-
enzymatic browning, collagen denaturation or oxidation of some chemical component;
while in the enamel, a observed white pattern is observed after thermal treatment occurs
because the water loss and consequent increase in the light scattering by the tissue.
Figura 7-(a) X-ray diffraction pattern of irradiated bovine enamel and main hydroxyapatite (HAP)
Bragg peaks. (b) The same experimental X-ray diffraction pattern including the main tetracalcium
phosphate (TetCP) Bragg reflection peaks.

Temperature rise due to CuHBr laser irradiation (λ=510 nm) in occlusal surface was
imaged using an IR thermographic camera. Figura 8 shows images for four instants (1 s (a),
5 s (b), 10 s (c) and 15 s (d)) during irradiation with 38.5 kW/cm2. The green marker
highlights areas where the temperature lies in the interval between 65o-70o C and suggests
isotropic heat propagation. Figura 9 shows the temperature profile along the line drawn in
the infrared image of the tooth irradiated with 5,7 kW/cm2. Image (a) refers to the initial
instant and (b), after 15 seconds of irradiation.

(a) (b)

(c) (d)

Figura 8: IR thermographic images of an human molar tooth during laser irradiation (λ=510 nm, 38.5
kW/cm2) for four different instants: (a) t=1 s, (b) t= 5 s, (c) t=10s and (d) t= 15 s. The green marker
highlights areas where the temperature lies in the interval between 65o-70o C and suggests isotropic
heat propagation.
 (a) (b)
Figura 9:Temperature profile along the line drawn in the infrared image of the tooth, for a sample
irradiated with 0.5 W. Image (a) refers to the initial instant and (b), to t=15 s.
As mentioned in Laser Engineering and Instrumentation activities, in vitro studies are
being made on enamel irradiated with high intensity ultrashort pulses (femtosecond and
picosecond) (Figura 10). Morphological, thermal and mass removal processes are under
investigation in both temporal regimes.

Figura 10- SEM of bovine enamel treated by 17 GW of peak power of λ= 830nm, 1 kHz, 60 fs ,1
mJ/pulse. As a result a track was created with a minimal thermal load in the process.

Optical Diagnostic Methods

- Optical proprieties of the caries lesion differ from sound tissue. The fluorescence is used
as a new optical diagnostic method for incipient caries, with higher sensibility and
specificity than the current clinical analysis. Our aim is to characterize the demineralization
and remineralization processes of enamel lesions by optical methods. The artificial lesions
will be analysed at different levels of demineralization and remineralization process by
spectroscopy, confocal microscopy and transverse microradiography.

- Optical Characterization Of Biological Tissue By Optical Coherence Tomography (OCT)

Technique: OCT is a new non invasive optical detection technique, based in a Michelson
interferometer. It was possible to produce a tomogram of dentine-enamel junction and the
OCT system accurately depicts dental tissue, being able to detect early caries in its structure
with a lesion depth of 50µm, analyzing the tomogram of the lesion. The principal problem
of this system was the linear translator that needs a high resolution and high precision
movements (<5 µm) Figura 12.
- Pulp Vitality Test By The Laser Doppler Flowmetry. A method to diagnose the pulp
vitality state was developed, using the laser Doppler flowmetry (Figura 11). The method is
based on the discrimination of an unconventional haemodynamic parameter, the percentage
variation of the pulp flow, defined as the percentage of the pulp blood flow variation of the
interrogated tooth when compared to a homologue one. This haemodynamic parameter is
less sensitive to factors widely known as drawbacks of the technique.
Figura 11 - Laser Doppler Flowmeter Probe fixed by a silicon splint to measure pulp blood flow.

Figura 12- Lesion tomogram representing an early tooth caries with a lesion depth of 50µm

Low Intensity Lasers –research

- Low-intensity laser therapy (LILT) on blood microcirculation during skin wound
healing was studied by laser Doppler flowmetry. The results indicated that there is
no immediate effect due to the laser radiation on microcirculation, although an
increasing of blood flow was observed on the 7th day post-wounding in irradiated
specimens. Also, skin samples irradiated with linearly parallel polarized He-Ne
laser beam relative to a preferential direction on animal’s back showed collagen
fibers more organized than control skin and burned skin samples presents a
significant higher attenuation coefficient than health skin samples. The attenuation
coefficient changes during skin wound repair process in a different manner if the
lesion is treated with laser or not.
- The findings in bone repair after titanium implant surgeries following LILT showed
that on the 21th and 42th days post-operatory, irradiated sites presented statistically
significant implant removal torque values higher than those from non-irradiated
sites.
 - Partial results in caries prevention in vivo using LILT associated to topic fluoride
indicated that there are no morphological and chemical changes on enamel surface
after laser therapy.
- The development of new photosensitizers and the improvement of existing ones for
antimicrobial photodynamic therapy (PDT), most of them for odontopathies are
under investigation. Studies on peri-implantitis in dogs suggest that PDT using
azulene is effective to kill periodontopathogenic bacteria.
-
Low Intensity Lasers clinical research
- Study about LILT in the prevention of oral mucositis in patients undergoing bone
marrow transplants was carried out. The results showed that the diode 650nm laser
decreased the severity of oral mucositis as well as the degree the pain when used as
a preventative therapy in patients undergoing bone marrow transplants. This work
was developed as a cooperation between São Paulo branch and São Carlos branch
of this CEPOF.
- Effects of LILT on the velocity of canine tooth movement and consequently bone
remodeling were investigated The results indicated that all patients showed
significant acceleration of the distalization velocity on the side treated with LILT
when compared to the control.
- Effects of LILT on the ultrastructure of dentine-pulp interface after conventionally
prepared class I cavity preparation were studied. The findings suggest that laser
irradiation accelerates the recovery of the dental structures involved in the cavity
preparation at the pre-dentine level.
- Results about LILT using λ= 660 nm and λ= 780 nm on hypersensitive dentine
showed that there are not statistical significant differences between wavelengths in
pain relief after treatment

High Intensity Lasers in Restorative Dentistry, Periodontics and General Dentistry

 In the study of caries prevention with Er,Cr:YSGG laser irradiation samples of
bovine enamel were irradiated with Er,Cr:YSGG laser at fluences of 2,8 J/cm2, 5,6 J/cm2
and 8,5 J/cm2. In these condition, the EDX analysis showed no significant changes in Ca/P
ratio; a maximum rinse of 1o C was detected at analysis of pulpar chamber temperature and
SEM observation showed some melted areas around microablation ones.
Adhesion and groth of cultured human gingival fibroblasts on root surfaces treated
by either irradiation with Er:YAG laser using a periodontal fiber or curet were compared,
showing that 3J/cm2 (twice) promoted faster adhesion and growth than surfaces treated with
either root planning or higher irradiation conditions.
Temperature rise was evaluated using an IR thermographic camera (ThermaCam
SC3000, Flir Systems, USA) on a tooth recovered (a) and unrecovered (b) with a H2O2
bleaching gel and irradiated with 27-46 W/cm2 of a diode laser (λ=805nm) during 30s.
Figura 13 shows the images for the highest intensity and results indicated that these
parameters are safe for teeth vitality and the temperature gradient is lower in the recovered
sample.

(a) (b)

Figura 13 :IR thermografic images of incisors recovered (a) and unrecovered (b) with bleaching gel and
irradiated with 46 W/cm2 of a diode laser (λ=805nm) during 30s. Temperature of spot SP01 in (a) is
29.8oC and 31.5oC in (b). Spot SP02 in (a) is 30.0oC and spot SP03 in (b) is 32.7oC.
To measure the pulp blood flow during orthodontic forces it was developed a new
method to investigate the supply state of the pulp microcirculation during and after
orthodontic forces using the laser Doppler flowmetry. The method is based on the analysis
of the percentage of pulp flow variation during the tooth displacement, compared to the
initial flow, prior to the force application. The method allow the flow to be investigated
during long periods, indicating that the flow is substantially reduced immediately after the
force is applied (due to the strangulation of vessels), and remains reduced during at least 72
hours due to the resulting pulp inflammation, induced by the periodontic inflammation
process.

Lasers in Endodontics

In the last past decade laser technology has been considered an efficient clinical tool
in Endodontic treatment. Many studies using different wavelengths have shown the
microbial reduction capacity of high intensity power laser irradiation and recently even the
low power laser energy used with the concept of photodynamic therapy, associated with
specific dyes interacting with specific wavelengths has been efficient for the elimination of
bacteria in infected root canals. Previous studies have established save laser parameters so
that the temperature on the external root surface during the irradiation does not increase
more than 10 0C avoiding thermal damage to surrounding tissues. Furthermore, many other
in vitro research pointed out the morphologic changes of dentine surface after high intensity
laser intra canal irradiation as well as the influence of those alteration to the sealing of root
filling by dye apical infiltration. Finally, studies of periapical surgeries with laser
technology have gave the support to the clinical use. The Er:YAG laser is optimized for
hard tissue removal, performing the soft tissue incision, the osteotomy and root apical
resection, as well as the high intensity diode and Nd:YAG lasers perform the microbial
disinfection in the surgical site.

Figura 14- Fiber inside root canal during Nd:YAG irradiation for microbial disinfection.
 Main Researchers
Carlos de Paula Eduardo (FOUSP)
Denise Maria Zezell (IPEN)
Gessé Eduardo Calvo Nogueira (IPEN)
Martha Simões Ribeiro (IPEN)

Collaborators
Armando Mirage (IPEN)
José Luiz Laje-Marques (FOUSP)
Márcia Martins Marques (FOUSP)
Martha Marques Vieira (IPEN)
Miriam Lacalle Turbino (FOUSP)
Niklaus Ursus Wetter (IPEN)
Nilson Dias Vieira Jr. (IPEN)
Sonia Licia Baldochi (IPEN)
Wagner de Rossi (IPEN)
Lilia Coronato Courrol
(FATEC/IPEN)
Edison Puig Maldonado
(USCamilo/IPEN)

Pos- docs
Sheila Gouw Soares FOUSP
José Eduardo P. Pelino FOUSP
Edgar Yuji Tanji FOUSP
Ilíria Salomão Feist FOUSP
 Students- IPEN Master (M)
PhD (D)
Adriana Costa Ribeiro D
Aécio Massayoshy Yamada D
Aguinaldo Silva Garcez Segundo D
Alvaro W R Salles D
Anderson Zanardi de Freitas D
Andrea Antunes D
Daniela de FátimaTeixeira da Silva D
Luciano Bachmann D
Silvia Cristina Pedreira Nunes D
Walter Miyakawa D

Cassius Guilherme Farina M

Fernanda de Paula Eduardo M
Flávia T O de Paula Eduardo M
Karin Praia Muller M
Patrícia da Ana M
Renata Malheiro e Rocha M
Ricardo Rada Ahmad Hayek M

Students- School of Dentistry (FOUSP)

Ana Cecília Correa Aranha D
Fábio Renato Pereira Robles D
José Guilherme Antunes Guimarães D
José Ricardo de Freitas Archilla D
José Trancoso Tannous D
 Luciane Hiramatsu Azevedo D
Patrícia Haypek D
Patrícia Moreira de Freitas D
Ricardo Scarparro Navarro D
Vinícius Rangel Geraldo Martins D
Ana L Nogueira M
Renata Rodrigues de Carvalho undergraduate
Fernanda Baudichon Domingues undergraduate

Students of Professional Master in Lasers in Dentistry

(IPEN/FOUSP)
Aline Pierote de Oliveira
Ana Cristina Cury Camargo
Andre Buainain
André Machado Senna
Arcelino de Miranda Lobato Neto
Arlindo L. Guimarães Filho
Carla Di Rago Todescan
Carlos Eduardo Carvalho Vilela
Cristiane Baggio Polido
Cynthia Miranda França
Cynthia Tannure Coelho Britto
Fernanda de Almeida Youssef
Francisco Meira Britto Junior
Glen A. Maia de Oliveira
Ismael Lucas Pinto
Jose luiz Feres Lucarelli
Leticia Lang
Márcia Cristina da S. Barroso
Maria Cristina Eiko Hashimoto
Maria da Graças Cruz Najar
Maria do Rosario Santos Freire
Matheus Sousa de Araújo
Miriam Tomaz de Magalhães
Paola Racy de Micheli
Patricia de Paula B.de Almeida
Regina Keiko Nakasone
 Renata Fronzaglia Lollato
Renato Araújo Prates
Rively de Oliveira Rodrigues
Silméa Zegaib
Silvia Cristina Tassinari
Vera Lúcia Kögler
Warthon Leite de Assis

Technicians
Haroldo Lima Bueno FOUSP
José Trt Vidal IPEN
Liliane de Souza e Souza FOUSP
Maria Aparecida Barbosa FOUSP

Dissertations

1) Silva, D.F.T., “Analysis of the relative attenuation coefficient and the collagen
birrefringence in health and burned skin irradiated with linearly polarized He-Ne
laser”, october, 2002- Advisor: Martha Simões Ribeiro- IPEN.
2) Eduardo F. P., “Effects of low-intensity laser radiation (650 nm and 780 nm) in the
prevention of oral mucositis in patients undergoing bone marrow transplant”,
march, 2003-Advisor: Denise Maria Zezell- IPEN.
3) Nogueira, A.L.F.- Laser Doppler as a diagnostic tool for pulp vitality- establishing
reading parameters, April, 2003- Advisor: José Luiz Lage Marques, FOUSP.
4) MEDA, Eduardo Monteiro. Estudo morfológico da dentina humana irradiada com
o laser de Er:YAG e sua relação com os cimentos de ionômero de vidro.
26/09/2002. Advisor: :Carlos de Paula Eduardo- FOUSP
5) RODE, K. M. Estudo da microdureza e profundidade de polimerização de resina
composta com uso do laser de argônio. 24/09/2002. Advisor: Miriam Lacalle
Turbino- FOUSP
 6) LLORET, P. R. Resistência de união à dentina de resinas compostas polimerizadas
com luz halógena e laser de argônio. 25/09/2002. Advisor: Miriam Lacalle Turbino-
FOUSP
7) ROBLES, F. R. P. Avaliação da resistência à tração da dentina bovina irradiada
com laser de Er:YAG combinada a diferentes tratamentos. Análise das superfícies
pela microscopia eletrônica de varredura. 17/03/2003. Advisor: Carlos de Paula
Eduardo- FOUSP
8) ARAKI, A. T. Análise da reparação óssea periapical pós-tratamento endodôntico,
tendo como variável a irradiação intracanal com laser de Nd:YAG. 21/03/2003.
Advisor: José Luiz Lage Marques- FOUSP

Thesis

1) Stolf, S.F. “Spectroscopic and thermal characterization of bovine enamel and

dentine using the photoacoustic spectroscopy”, February, 2003-Advisor: Martha
M.F. Vieira, Co-Advisor: Denise Maria Zezell- IPEN
2) PELINO, Jose Eduardo Pelizon. Estudo in vitro das propriedades ópticas, químicas
e cor do esmalte e dentina em diferentes condições, e irradiados com lasers de
diferentes comprimentos de onda. 19/09/2002. Advisor: Carlos de Paula Eduardo-
FOUSP
3) BEVILACQUA, Flávia Magnani. Estudo in vitro do laser de Er:YAG sem
refrigeração sobre a superfície de esmalte e dentina: análise morfológica e efeitos
térmicos. 10/10/ 2002. Advisor: Carlos de Paula Eduardo- FOUSP.
4) BISPO, L. B. Avaliação da profundidade de condicionamento do esmalte dental
bovino após irradiação com o Er:YAG laser: estudo in vitro. 12/09/2002; Advisor:
Carlos de Paula Eduardo- FOUSP.

Dissertations from the Professional Master Course- Lasers in Dentistry

1. Name: Duilio Naves Junqueira Junior

Conclusion:19/11/2002
Advisor: Prof. Dr. Carlos de Paula Eduardo
Co-advisor: Profa. Dra. Martha Marques F. Vieira
Dissertation: Influence of the incidence angle on the morphology of enamel and dentin
under Er:YAG laser irradiation

2. Name: Angelo Maércio Finochio Junqueira

Conclusion:19/11/2002
Advisor: Profa. Dra. Denise Maria Zezell
Co-advisor: Prof. Dr. José Luiz Lage-Marques
Dissertation: Evaluation of microleakage occurred in class V restoration prepared with
ER:YAG laser and also with high speed, restored using composite

3. Name: Silvia Cristina Núñez

Conclusion:12/12/2002
Advisor: Prof. Dr. José Luiz Lage-Marques
Co-advisor: Prof. Dr. Gesse Eduardo Calvo Nogueira
Dissertation: He-Ne laser effects on blood microcirculation an in vivo study through
laser Doppler flowmetry

4. Name: Luís Mário De Melo Lopes

Conclusion:13/12/2002
Advisor: Prof. Dr. Niklaus Ursus Wetter
Co-advisor: Prof. Dr. Carlos de Paula Eduardo
Dissertation: Clinical study on the removal of gingival melanin pigmentation:
comparison between Nd:YAG laser ablation and mechanical abrasion

5. Name: Fábio Ribeiro De Souza

Conclusion:10/02/2003
Advisor Prof. Dr. Nilson Dias Vieira Jr.
Co-advisor: Prof. Dr. Walter D. Niccoli Filho
Dissertation: Resistance fracture in rat femur irradiated with CO2

6. Name: Bruno Miranda Godoi

Conclusion:19/03/2003
Advisor: Prof..Dr. Martha Simões Ribeiro
Co-advisor: Prof. Dr.
Dissertation: Effects of low-intensity GaAlAs laser radiation (λ=660nm) on dentine-
pulp interface after class I cavity preparation

7. Name: Rosely Cordon

Conclusion:27/03/2003
Advisor: Prof. Gessé Eduardo Calvo Nogueira
Co-advisor r: Prof. Dr. Carlos de Paula Eduardo
Dissertation: Evaluation of the ocular protection for low intensity therapeutic lasers

8. Name: Sun Chien Yuan

Conclusion:07/04/2003
Advisor: Prof. Dr. Wagner de Rossi
Co-advisor: Prof. Dr. José Luiz Lage Marques
Dissertation: Comparative analysis of the effect of the GaAlAs laser irradiation in 780
nm 660 nm in the hypersensitive dentin

9. Name: Wendell Lima De Carvalho

Conclusion: 08/04/2003
Advisor: Prof. (a) Dr.(a) Denise Maria Zezell
Co-advisor: Prof. Dr.Carlos de Paula Eduardo
Dissertation: In vitro evaluation of marginal microlekage in class V restorations with
composite resin in bovine teeth. Laser irradiation influences and the adhesive system in
the dentin pre-treatment

10. Name: Mauricio Gulin

Conclusion:10/04/2003
Advisor: Prof.(a) Dr.(a) Sheila Cynthia Gouw Soares
Co- orientador: Prof. Dr. Armando Mirage
Dissertation: Root surfaces modification after Diode Laser irradiation

11. Name: Maria Aparecida Toda

Conclusion:09/04/2003
Advisor: Prof. Dr. Wagner de Rossi
Co-advisor: Prof. Dr. Edmir Matson
Dissertation: Comparing study in vitro to sealant, pit and fissure with the Nd:YAG
and/or phosphoric acid 37% and glass ionomer cement (Fuji IX) with second
mandibular temporary molars

12. Name: Delma Rebelo

Conclusion:10/04/2003
Advisor: Prof. Dr. Niklaus Ursus Wetter
Co-advisor: Prof. Dr. Edmir Matson
Dissertation: Effects of low-energy laser irradiation on the distalization velocity during
experimental canine tooth movement in humans: comparative clinical study

13. Name: Thyrso Castilho Filho

Conclusion:10/04/2003
Advisor: Prof.(a) Dr.(a) Denise Maria Zezell
Co-advisor: Prof. Dr. Eduardo B. Groth
Dissertation: Evaluation of the effect of low intensity laser irradiation on the
osseointegration of titanium implants inserted in rabbits tibia
14. Name: Patricia Ebel
Conclusion:11/04/2003
Advisor: Prof.(a) Dr.(a) Sonia Licia Baldochi
Co-advisor: Prof. Dr. Eduardo de Bortoli Groth
Dissertation: “In vitro study of the diode laser effect on artifical demineralized surface
or human dental enamel”.

15. Name: Jose Antonio Gaspar

Conclusion:11/04/2003
Advisor: Prof. Dr. Armando Mirage
Co-advisor: Prof. Dr. Carlos de Paula Eduardo
Dissertation: “Evaluation of new dyes and hydrogen peroxide irradiated with argon or
diode laser in dental bleaching in vitro”.

16. Name: Thatyana Carla Ciasca Dos Santos

Conclusion:15/04/2003
Advisor: Prof. Dr. Wagner de Rossi
Co-advisor: Prof.(a) Dr.(a) Sheila C. Gouw Soares
Dissertation: “Laser parameters control for dentinal tubules sealing”

17. Name: Vinicícius Rangel Geraldo Martins

Conclusion:26/06/2003
Advisor: Prof. Dr. Carlos de Paula Eduardo
Co-advisor: Prof. Dr. Niklaus Ursus Wetter
Dissertation: “Intrapulpar temperature during preparation with the Er: YAG laser:na in
vitro study”

18. Name: Alessandra De Siervi Mattos

Conclusion:16/07/2003
Advisor: Prof. Dr. Niklau Ursus Wetter
Co-advisor: Prof Dr. José Eduardo Pelizon Pelino
Dissertation: “In vitro evaluation of the technical and morfological change of the
enamel to surface and using different bleaching technique”

19. . Name: Breno Carnevalli Franco De Carvalho

Conclusion:30/07/2003
Advisor: Prof. Dr. José Luiz Lage Marques
Co-advisor: Profa Dra. Lilia Coronato Courrol
Dissertation: “Exogenous bleaching evaluation on dentin using chemical activated
technique compared with diode laser technique”

20. Name: Cátia Cilene Nass Sebrão

Conclusion:31/07/2003
Advisor: Prof. Dr. Edison Puig Maldonado
Co-advisor: Profa Dra. Sheila C. Gouw Soares
Dissertation: “Dentine surface evaluation of root canals irradiated in vitro with Er:
YAG laser”
 5) Published Articles:

1. Bachmann L, Diebolder R, Hibst R, Zezell DM : Infrared Absorption bands of enamel

and dentin tissues from human and bovine teeth. Appl Spectrosc Rev 38:(1) 1-14
(2003).

2. Bachmann L, Zezell DM, Diebolder R e Hibst R. Infrared spectroscopy of dentin

irradiated by erbium laser. Elsevier Int. Congr. Ser. 1743:1-4 (2003).

3. Bachmann L, Zezell DM, Maldonado EP: Determination of beam width and quality for
pulsed lasers using the knife-edge method. Instrum Sci Technol 31:(1) 49-54 (2003).

4. Theodoro LH, HaypekP, Bachmann L, Garcia VG, Sampaio JEC e Eduardo CP. Effect
of Er:YAG and Diode laser on the root surface. Morphological and thermal analyses.
Journal of Periodontology 74(6): 838-843 (2003).

5. Schein M, Bocangel J S, Nogueira G E C, Schein P A L, “SEM evaluation of the

interaction pattern between dentin and resin after cavity preparation using Er:YAG
laser”, Journal of Dentistry, In Press 2003.

6. Ribeiro, M.S.; Silva, D.F.T.; Pellegrini, C.M.R.; Oliveira, S.F.; Zorn, T.M.T. and
Zezell, D.M. "Effects of low-intensity polarized visible laser radiation on skin burns. A
light microscopy study”. J. Lasers Surg. Med. Accepted 20/02/2003.

7. Nuñez, S.C.; Ribeiro, M.S.; Nogueira, G.E.C.; Lage-Marques, J.L. “Efeito do laser de
He-Ne na microcirculação sanguínea – estudo in vivo por meio de fluxometria laser
Doppler. Pesq. Odontol. Brasileira 17(S.2), p. 105, 2003.

8. Garcez, A.S.; Souza, F.R.; Nuñez, S.C.; Kather, J.M.; Ribeiro, M.S. “Terapia
Fotodinâmica em Odontologia – laser de baixa potência para redução microbiana”.
Revista da APCD 57(3), 223-226, 2003.

9. Sanseverino, N.T.M.; Sanseverino, C.A.M.; Groth, E.B.; Ribeiro, M.S. “Avaliação

Clínica da ação antiálgica do laser em baixa intensidade de GaAlAs (λ= 785 nm)no
tratamento das disfunções da articulação têmporo-mandibular”. ABLO news 1(1), 6-12,
 2002.

10. Rodrigues, M.T.; Ribeiro, M.S.; Groth, E.B.; Zezell, D.M. “Efeito terapêutico do laser
de GaAlAs sobre lesões provocadas pelo aparelho ortodôntico fixo na cavidade oral”.
ABLO news 1(2), 8-11, 2002.

11. Garcez, A.S.; Jorge, A.O.C.; Lage-Marques, J.L.; Ribeiro, M.S. “Redução bacteriana
intracanal com laser em baixa intensidade (685 nm). Estudo in vitro”. ABLO news
1(3), 10-13, 2003.
12. Garcez, A.S.; Jorge, A.O.C.; Lage-Marques, J.L.; Ribeiro, M.S. “Desinfecção de canais
com laser de emissão vermelha em baixa intensidade associado a fotossensibilizador.
Estudo in vitro”. Anais do 21O Congresso Internacional de Odontologia de São
Paulo, CD-ROM, 2003.
13. Gekelman, D.; Prokopowitsch, I.; Eduardo, C.P. In Vitro Study of the Effects of
Nd:YAG Laser Irradiation on the Apical Sealing of Endodontic Fillings Performed with
and without Dentin Plugs. Journal of Clinical Laser Medicine & Surgery, New York,
v. 20, n. 3, p. 10-20, 2002.

14. Matson, J.R.; Matson, E.; Navarro, R.; Eduardo, C.Paula. Er:YAG Laser Effects on
Enamel Occlusal Fissures: An in Vitro Study. Journal of Clinical Laser Medicine &
Surgery, New York, v. 20, n. 1, p. 27-35, 2002.
15. Baraldi, Carlos Eduardo; Puricelli, Edela; Eduardo, Carlos de Paula. Superficial
Smoothness of Resesction Suface after Apicoectomy and Nd:YAG Noncontact Laser
Irradiation: An In Vitro Study. Journal Of Oral Laser Applications, New Malden, v. 2,
n. 1, p. 17-24, 2002.
16. Ribeiro, M.S.; Silva, D.F.T.; Pellegrini, C.M.R.; Oliveira, S.F.; Zorn, T.M.T. And
Zezell, D.M. Effects of low-intensity polarized visible laser radiation on skin burns. A
light microscopy study. J. Clin. Laser Surg. Med. 2003
17. F. A Kawaguchi ; C. P. Eduardo ; A B. Matos . Nd:YAG Laser Influence on
Microleakage of Class V Composite Restoration. Journal of Clinical Laser Medicine
& Surgery, Estados Unidos, v. 21, n-4, p-227-229, 2003.
18. Jacobs, Maria Margarete Rodrigues Palomares; Matson, Edmir; Eduardo, Carlos De
Paula; Oda, Margareth. Action of the Nd:YAG Laser in Lactobacillus Culture: An In
Vitro Study. Journal Of Oral Laser Applications, New Malden, v. 3, n. 2, p. 87-91,
2003.
19. Feist, Ilíria Salomão; Micheli, Giorgio De; Carneiro, Silvia R S; Eduardo, Carlos De
 Paula; Miyagi, Sueli P H; Marques, Márcia Martins. Adhesion and Growth of Cultured
Human Gingival Fibroblasts and Periodontally Involved Root Surfaces Treated by
Er:YAG Laser. Journal of Periodontology, Estados Unidos, v. 74, n. 9, p. 1368-1378,
2003.
20. Pereira, Aymann Nassif; Eduardo, Carlos De Paula; Matson, Edmir; Marques, Márcia
Martins. Effect of low-power laser irradiation on cell growth and procollagen synthesis
of cultured fibroblasts. Lasers In Surgery And Medicine, Estados Unidos, v. 31, n. 4,
p. 263-267, 2002.
21. Carvalho, Cláudio Antonio Talge; Valera, Márcia Carneiro; Gouwsoares, Sheila;
Eduardo, Carlos De Paula. Effects of Nd:YAG and Er:YAG lasers on the sealing of root
canal fillings. Journal of Clinical Laser Medicine & Surgery, New York, v. 20, n. 4,
p. 215-219, 2002.
22. Bachmann L, Santos AB, Baffa O, Zezell DM. ESR native signal in thermally treated
dental tissue. (accepted at 19/07/2003) Spectroscopy Letters.
23. Bachmann L, Sena ET, Stolf SF, Zezell DM. Discoloration of hard dental tissues after
thermal treatment (accepted at 04/08/2003) Archs. Oral Biol.
24. Geraldo-Martins, V.; Vieira, M.M.F.; Eduardo, C.P.- Intrapulpar Temperature during
cavity preparation with Er:YAG. (accepted 17/09/2003) Journal of Clinical Lasers in
Medicine and Surgery.

Published Articles - Abstracts

1. Blay, A., Blay, C. C., Groth, E. B., Zezell, D. M.- Effects of visible and nir low
intensity lasers on implant osseointegration in vivo. Lasers In Surgery And Medicine.
E.U.A: , n.14, p.12 - 12, 2002
25. Marraccini, T. M., Wigdor, H. A., Turbino, M. L., Stabholtz, A., Zezell, D.M. Laser-
Etched Conditioning of Dental Hard Tissues Using The Er:YAG Laser And CO2 Laser.
Lasers In Surgery And Medicine. v 30(suppl.14), p.73 - 74, 2002
2. Rodrigues, M. T. J., Ribeiro, M. S., Groth, E. B., Zezell, D. M. Evaluation of effects of
Laser Therapy Lambda=830nm On Oral Ulceration Induced By fixed Orthodontic
Appliances. Lasers In Surgery And Medicine v 30(suppl.14),, p.15 - 15, 2002
3. Ribeiro, M. S., Silva, D. F. T., Freitas, A. Z., Pellegrini, C. M. R., Oliveira, S. F.,
Araújo, C. E. N., Zorn, T. M. T., Zezell, D. M. Interaction of Low-Intenity Linearly
Polarized Laser Radiation With Living Tissues: Effects on Tissular Acceleration of
Skin Wound Healing. Lasers In Surgery And Medicine. v 30(suppl.14), p.13 - 13,
2002
4. S.B. Rezende, M.S. Ribeiro, V.G. Garcia, E.P. Maldonado, "Histological and
Biometrical Comparison Among Three Irradiation Configurations for Skin Wound
Healing Acceleration in Rats Using an 830nm Diode Laser". Lasers Surg. Med.
 30(suppl.14): 13, 2002
5. D.M. Rocha, M.S. Ribeiro, C.P. Eduardo, "Clinical and Morphologic Evaluation of
Er:YAG Laser Action at the Front of Cervical Dentinal Hipersensitivity". Lasers Surg.
Med. V 30(suppl.14): 80, 2002
6. Sawasaki, M.S. Ribeiro, L.T. Mizuno, T. Akatsu, R. Sawasaki, "A Comparative Study
of the Effect of Low Laser Radiation on Mast Cells in Inflammatory Fibrous
Hyperplasia Colored and Not Colored by the Toluidine Blue". Lasers Surg. Med. V
30(suppl.14), 81, 2002
7. M.S. Ribeiro, D.F.T. Silva, A.Z. Freitas, C.M.R. Pellegrini, S.F. Oliveira, C.E.N.
Araújo, T.M.T. Zorn, D.M. Zezell, "Interaction of Low-Intensity Linearly Polarized
Laser Radiation with Living Tissues: Effects on Tissular Acceleration of Skin Wound
Healing". Lasers Surg. Med. V 30(suppl.14), 13, 2002
8. N.V.G. Pulga, F.G. Pulga, R.C. Ribeiro, M.S. Ribeiro, A. Ramos, M.I. Turbino, R.S.
Navarro, M.M.F. Vieira, C.P. Eduardo, "Marginal Microleakage Evaluation in Class V
Restorations of Deciduous Teeth Prepared Conventionally and Using Er:YAG Laser".
Lasers Surg. Med. 30(suppl.14), 81, 2002
9. G.R. Sousa, M.S. Ribeiro, E.B. Groth, Bone Repair of the Periapical Lesions Treated or
Not with Low-Intensity Laser (λ= 904nm). Lasers Surg. Med. 30(suppl.14), 81, 2002
10. L.B. Silveira, M.S. Ribeiro, A.A. Garrocho, M.D. Novelli, H.A. Marigo, E.B. Groth,
"In VivoStudy on Mast Cells Behavior Following Lowe-Intensity Visible and Near
Infrared Lasser Radiation", Lasers Surg. Med. 30(suppl.14), 82, 2002
11. N.T.M. Sanseverino, C.A.M. Sanseverino, M.S. Ribeiro, E.B. Groth, "Clinical
Evaluation of the Low-Intensity Laser Antialgic Action of GaAlAs (λ= 785nm) in the
treatment of the Temporomandibular Disorders". Lasers Surg. Med. 30(suppl.14), 18,
2002
12. .C.F. Amorim, M.S. Ribeiro, E.B. Groth, "Gingival Healing after Gingivectomy
Procedure and Low-Intensity Laser Irradiation: A Clinical and Biometrical Study In
Anima Nobile". Lasers Surg. Med. 30(suppl.14), 20, 2002
13. NAVARRO, R.S.; YOUSSEF, M.N.; EDUARDO, C.P.; MATOS, A.B.. Bond strength
to enamel and dentin conditioned by Er:YAG laser and self-etching adhesive. Journal
of Dental Research, v. 81, n. Spec Iss A, p. A-76-A-76, 2002.
14. CALMON, Andreia de Avillez; GROTH, Eduardo de Bortoli; MIRAGE, Armando;
EDUARDO, Carlos de Paula. Diamine silver fluoride as a photo-absorber for the
Nd:YAG laser irradiation on temporary teeth with caries like lesions. Journal of Dental
Research, v. 81, n. Spec Iss A, p. A-98-A-98, 2002.
15. PELINO, Jose Eduardo Pelizon; ZEZELL, Denise Maria; EDUARDO, Carlos de Paula;
WHITE, Joel. In vitro study of the optical, chemical and color properties of enamel and
dentin in different conditions and irradiated with lasers with different wavelengts.
Journal of Dental Research, v. 81, n. Spec Iss A, p. A-194-A-194, 2002.
16. RAMOS, Andreia; PULGA, Neusa Vieira Galvão; VIEIRA, Martha; EDUARDO,
Carlos de Paula. Microleakage in class V restorations of deciduous teeth prepared with
Er:YAG laser. Journal of Dental Research, v. 81, n. Spec Iss A, p. A-245-A-245,
2002.
17. NAVARRO, Ricardo Scaparro; YOUSSEF, Michel Nicolau; EDUARDO, Carlos de
Paula; MATOS, Adriana Bona. Morphological analysis of enamel and dentin surfaces
conditioned by Er:YAG laser and self-etching primer. Journal of Dental Research, v.
81, n. Spec Iss A, p. A-280-A-280, 2002.
18. LAGE-MARQUES, José Luiz; EDUARDO, Carlos de Paula; ARAKI, Angela T.
Treatment of endodontic overfiling by Nd:YAG laser. Journal of Dental Research, v.
81, n. Spec Iss A, p. A-321-A-321, 2002.
19. Effects of copper vapour laser radiation in the root canal wall dentine M.C.G. Silveira,
W.D. Niccoli, N.D. Vieira e R. Riva Journal of Dental Research , 81, B203-B203, issue
B, dec2002

Presentation in international meetings

1) Walverde, D.A.; Quintiliano, T.L.; Pires, Luiz Antônio G.; Wetter, N.U.
Comparative study of two different bleaching agents activated by xenon arc lamp
and 960 nm diode laser. In: 16th Scientific Meeting Of The European Academy Of
Esthetic Dentistry, 2002 Eaed Meeting, 2002, Istambul. 2002 Istambul Meeting.
2) FEIST, Ilíria Salomão; CARNEIRO, S R S; EDUARDO, Carlos de Paula;
MIYAGI, S P H; MARQUES, Márcia Martins. Adhesion and growth of gengival
fibroblasts and root surfaces treated with Er:YAG laser. In: ESOLA - 2ND
UROPEAN SOCIETY FOR ORAL LASER APPLICTIONS, Firenze. ESOLA -
2nd European Society for Oral Laser Applications. Berlin: Quintessenz Verlags-
GmbH, 2003. v. 1.
3) BLAY, Cláudia Campos; BLAY, Alberto; NASCIMENTO, Carlos Manuel de
Oliveira; EDUARDO, Carlos de Paula. Comparative analysis of bacterial decrease
by Er:YAG laser radiation an by a tip mounted on a high otation device after
removal of carious tissue dentine: study in anima nobile. In:
INTERNATIONALER JAHRESKONGRESS - 12TH INTERNATIONAL
 CONGRESS, 2003, Berlin. Internationaler Jahreskongress - 12th International
Congress. 2003. v. 1, p. 24-24.
4) MARQUES, Márcia Martins; HAYPEK, Patrícia; EDUARDO, Fernanda de Paula;
MIGLIORATI, César Augusto; ZEZELL, Denise Maria; EDUARDO, Carlos de
Paula. Effect of hight intensity diode laser irradiation on growth of human gingival
fibroblasts. In: ESOLA - 2ND EUROPEAN SOCIETY FOR ORAL LASER
APPLICTIONS, 2003, Firenze. ESOLA - 2nd European Society for Oral Laser
Applications. Berlin: Quintessenz Verlags-GmbH, 2003. v. 1.
5) MARQUES, Márcia Martins; PEREIRA, A N; FUJIHARA, N A; NOGUEIRA, F
N; MATSON, Edmir; EDUARDO, Carlos de Paula. Effect of low-power laser
irradiation on protein synthesis and ultrastructure of human gingival fibroblasts. In:
ESOLA - 2ND EUROPEAN SOCIETY FOR ORAL LASER APPLICTIONS,
2003, Firenze. ESOLA - 2nd European Society for Oral Laser Applictions. Berlin:
Quintessenz Verlags-GmbH, 2003. v. 1.

Published Articles- National Journals

1. THEODORO, Letícia Helena; GARCIA, Valdir Gouveia; HAYPEK, Patrícia,

ZEZELL, Denise Maria, EDUARDO, C. P. Análise morfológica, através de
microscopia eletrônica de varredura, da ação do laser de Er:YAG em superfícies
radiculares submetidas à raspagem e aplainamento radicular. Revista Pesquisa
Odontológica Brasileira, São Paulo, v.16, n.4, p. 308-312, 2002
2. FARINA, C G; EDUARDO, C P; ZEZELL, D M. Análise morfológica de sulcos
em esmalte dental irradiado com laser de neodímio. Revista Brasileira de Pesquisa
e Desenvolvimento, São Paulo, v.4, n.1, p.32-37, 2002.
3. WETTER, N.U.; OLIVEIRA, M.V.; KATO, I.T.; ZEZELL, D.M.; EDUARDO,
C.P.; VIEIRA JUNIOR, N.D.. Estudo in vitro das alterações morfológicas do
esmalte dentário irradiado com laser de diodo de alta potência: análise por
microscopia eletrônica de varredura. Revista Brasileira de Pesquisa e
Desenvolvimento, São Paulo, v. 4, n. 1, p. 14-19, 2002.
4. EDUARDO, Carlos de Paula; TANJI, Edgar Yuji; GOUWSOARES, Sheila
Cynthia. A Odontologia e o laser. In: VANZILLOTA, Paulo Sérgio;
GONÇALVES, Almiro Reis; SALGADO, Luiz Paulo dos Santos. (Org.).
Odontologia Integrada Atualização Multidisciplinar para Clínico e o Especialista.
 Rio de Janeiro, 2002, v. 1, p. 403-425.
5. EDUARDO, Carlos de Paula; GOUWSOARES, Sheila; HAYPEK, Patrícia.
Utilização clínica dos lasers. In: CARDOSO, Rielsen José Alves; GONÇALVES,
Elenice Aparecida Nogueira. (Org.). ODONTOLOGIA. São Paulo, 2002, v. 1, p.
441-461.

Published Articles- National Journals- abstracts

1. Kohara, E.K.; Myaki, S.I.; Wetter, N.U. Efeitos da irradição do laser de alta potência
sobre o esmalte de dentes decíduos. In: 19a Reunião Anual Da Sociedade Brasileira De
Pesquisa Odontológica, 2002, Águas de Lindóia - SP. Pesquisa Odontológica
Brasileira. 2002. v. 16, p. 41-41.
2. Kato, I.T.; Wetter, N.U. Determinação da eficácia do tratamento diodo laser e Duraphat
para fins preventivos através da análise pelo EDS. In: 19a Reunião Anual Da Sociedade
Brasileira De Pesquisa Odontológica, 2002, Água de Lindóia - SP. Pesquisa
Odontológica Brasileira. 2002. v. 16, p. 224-224.
3. S.B. Rezende, M.S. Ribeiro, V.G. Garcia, E.P. Maldonado, “Ação do diodo laser sobre
o processo de cicatrização de lesões cutâneas: estudo biométrico e histológico em
ratos”, Pesquisa Odontológica Brasileira v.16, p.118, 2002
4. S.B. Rezende, M.S. Ribeiro, V.G. Garcia, E.P. Maldonado, “Ação do diodo laser
emitindo em 830 nm sobre o processo de cicatrização de lesões cutâneas: estudo
biométrico e histológico em ratos”, Revista da Associação Paulista de Cirurgiuões
Dentista, v. 56, p.23, 2002
5. N.T.M. Sanseverino, C.A.M. Sanseverino, M.S. Ribeiro, E.B. Groth, “Avaliação clínica
da ação antiálgica do laser em baixa intensidade de GaAlAs (785 nm) no tratamento das
disfunções da articulação têmporo-mandibular”, ABLO News, v.1, p. 6-12, 2002
6. M.T.J. Rodrigues, M.S. Ribeiro, E. B. Groth and D.M. Zezell, “Efeito do laser em baixa
intensidade em feridas provocadas pelo aparelho ortodôntico”, Revista da Associação
Paulista de Cirurgiuões Dentista, v. 56, p.18, 2002
7. M.T.J. Rodrigues, M.S. Ribeiro, E. B. Groth and D.M. Zezell, “Lesões de mucosa
causadas por aparelhos ortodônticos: comparação entre terapia convencional e laser em
baixa intensidade”, Pesquisa Odontológica Brasileira v.16, p.139, 2002
8. Theodoro, LH; Zezell, DM; Haypek, P; Bachmann, L; Sampaio, JEC; Eduardo, CP.
Efeito do laser de diodo sobre a superfície radicular- Análise termica. Pesqui Odontol
Bras, v.17, suplemento 2 (Anais da 20 reunião anual da SBPqO)p. 242, 2003.
9. Feist, Ilíria Salomão; Carneiro, S R S; Micheli, Giorgio; Eduardo, Carlos De Paula;
Miyagi, S P H; Marques, Márcia Martins. Adesão e proliferação de fibroblastos
de gengiva humana cultivados sobre superfícies radiculares tratadas com Er:YAG.
Pesquisa Odontológica Brasileira, São Paulo, v. 16, n. Supl 2002, p. 122-122, 2002.
10. Pelino, José Eduardo Pelizon; Haypek, Patrícia; Gouwsoares, Sheila Cynthia;
Bachmann, Luciano; Zezell, Denise Maria; Eduardo, Carlos De Paula. Análise do
comportamento térmico do laser de diodo no esmalte, dentina e cemento dental - estudo
in vitro. Pesquisa Odontológica Brasileira, São Paulo, v. 16, n. Supl 2002, p. 15-
15, 2002.
11. Eduardo, Carlos De Paula; Bispo, Luciano Bonatelli; Matson, Edmir. Avaliação da
percolação em cavidades proximais preparadas com a técnica do mm em chanfro e
condicionadas com laser. Pesquisa Odontológica Brasileira, São Paulo, v. 16, n. Supl
2002, p. 153-153, 2002.
12. Oliveira, Rodrigo Guerra De; Baldochi, Sônia Lícia; Gouwsoares, Sheila Cynthia;
Eduardo, Carlos de Paula. Avaliação da permeabilidade e da morfologia apical após
apicectomia, tratamento e retropreparo com laser Er:YAG e Nd:YAG. Pesquisa
Odontológica Brasileira, São Paulo, v. 16, n. Supl, 2002, p. 131-131, 2002.
13. Haypek, Patrícia; Bachmann, Luciano; Pereira, Andreia Antunes; Zezell, Denise
Maria; Eduardo, Carlos De Paula. Avaliação da variação de temperatura em superfícies
de implantes irradiados com laser de CO2. Pesquisa Odontológica Brasileira, São
Paulo, v. 16, n. Supl 2002, p. 257-257, 2002.
14. Garcia, L G; Barbosa, M T; Tanji, Edgar Yuji; Eduardo, Carlos De Paula. Efetividade
do selamento oclusal convencional e com laser de Er:YAG - estudo in vitro. Pesquisa
Odontológica Brasileira, São Paulo, v. 16, n. Supl 2002, p. 110-110, 2002.
15. Guimarães, José Guilherme Antunes; Poskus, Laisa Tatiana; Silva, Eduardo Moreira
Da; Eduardo, Carlos De Paula; Zezell, Denise Maria. Estudo piloto sobre o aumento da
temperatura pulpar decorrente do clareamento a laser. Pesquisa Odontológica
Brasileira, São Paulo, v. 16, n. Supl 2002, p. 247-247, 2002.
16. Tanji, Edgar Yuji; Eduardo, Carlos De Paula; Yamada Jr, Aécio M; Matsumoto, K.
Estudo termográfico da variação de temperatura em dentina irradiada com o laser de
Er:YAG. Pesquisa Odontológica Brasileira, São Paulo, v. 16, n. Supl 2002, p. 243-
243, 2002.
17. Nunez, Silvia Cristina; Cordon, Rosely; Nogueira, Gessé Eduardo Calvo; Eduardo,
Carlos De Paula. Medição da potência de saíde do feixe de equipamentos laser em
baixa intensidade. Pesquisa Odontológica Brasileira, São Paulo, v. 16, n. Supl 2002,
p. 239-239, 2002.
18. Navarro, Ricardo Scarparro; Matos, Adriana Bona; Bocangel, J; Eduardo, Carlos De
Paula; Youssef, Michel Nicolau. Resistência adesiva e morfologia do esmalte e dentina
condicionados com laser de Er:YAG e sistema autocondicionante. Pesquisa
Odontológica Brasileira, São Paulo, v. 16, n. Supl 2002, p. 248-248, 2002.
19. Vasconcelos, B C; Cruz, S M L; Aranachavez, V E; Dorini, A L; Eduardo, Carlos De
Paula; Ramos, A.C.B. Resistência de união de dois sistemas adesivos em dentina
tratada com laser de Er:YAG. Pesquisa Odontológica Brasileira, São Paulo, v. 16, n.
Supl 2002, p. 109-109, 2002.
20. Freitas, A.Z.; Ribeiro, A.C.; Gomes, A.S.L.; Zezell, D.M.; Petrov,D.; Vieira, N.D.
"Construção de imagens de dentes humanos cariados através da tomografia por
coerência óptica" Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003, p. 201,
2003.
21. Martinez, E. F., Robles, F. R. P., Azambuja Jr, N., Tagami, J., Eduardo, C. P. Análise
em MEV das superfícies dentinárias irradiadas com laser de Er:YAG combinada a
diferentes tratamentos. Pesquisa Odontológica Brasileira, v.17, n.Supl 2, 2003, p.192
- 192, 2003.
22. Robles, F., Martinez, E. F., Tagami, J., Eduardo, C. P.- Avaliação da resistência à
tração da dentina bovina irradiada com laser de Er:YAG combinada a diferentes
tratamentos. Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.192 - 192,
2003.
23. Eduardo, F. P., Haypek, P., Zezell, D. M., Eduardo, C. P., Marques, M. M.- Efeito da
radiação com laser de diodo (808 nm) desfocado sobre a proliferação de fibroblastos de
gengiva humana. Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.242 -
242, 2003.
24. Theodoro, L. H., Zezell, D. M., Haypek, P., Bachmann, L., Sampaio, J. E. C., Eduardo,
C. P. Efeito do laser de diodo sobre a superfície radicular - análise térmica. Pesquisa
Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.242 - 242, 2003.
25. Domingues, F. B., Franco Vo, Eduardo, C. P. Efeitos do laser de Er:YAG e Nd:YAG
sobre a superfície radicular na hipersensibilidade dentinária - um estudo in vitro.
Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.73 - 73, 2003.
26. Martins, V. R. G., Tanji, E., Wetter, N. U., Nogueira, R. D., Eduardo, C. P. Estudo in
vitro da variação da temperatura intrapulpar de incisivos bovinos irradiados com laser
de Er:YAG. Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.188 - 188,
2003
27. Carvalho, W. L., Navarro, R. S., Tanji, E. Y., Zezell, D. M., Eduardo, C. P. Influência
do laser de Er:YAG e sistema adesivo autocondicionante na microinfiltração de
restaurações de compósitos. Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003,
p.239 - 239, 2003.
28. Ana, P. A., Blay, A., Blay, C. C., Eduardo, C. P., Zezell, D. M. Morfologia superficial
do esmalte após a irradiação do laser de Er,Cr:YSGG com baixas fluências. Pesquisa
Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.106 - 106, 2003.
29. Barros, R. X., Robles, F. R. P., Figueira Ax, Eduardo, C. P.
O som da ablação do tecido dental a laser de Er:YAG como possível parâmetro de
Odontologia Conservadora. Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003,
p.74 - 74, 2003.
30. Fonoff, R. N., Watanabe, I., Eduardo, C. P.- Reaparação tecidual da mucosa bucal de
ratos submetidos a frenectomia labial com laser de CO2: estudo ao microscópio de luz.
Pesquisa Odontológica Brasileira. , v.17, n.Supl 2, 2003, p.210 - 210, 2003.
AWARDS
Imaging carious human dental tissue with optical coherence tomography, Freitas1, A.Z;
Gomes2, A.S.L.; Ribeiro2, A.C*; Petrov3, D.V.; Vieira1, N.D.; Zezell1, D.M. - In 20th annual
meeting of Brazilian Society of Odontology Research. (SBPqO), Águas de Lindóia-SP,
Brazil, setembro de 2003. Award for best research in the session: Diagnosis Systems.

PATENT

National Patent: “Azulene paste for Photodynamic Therapy”

Ownership: Comissão Nacional de Energia Nuclear
Inventors: Aguinaldo Silva Garcez e Martha Simões Ribeiro
March 2003.

COLLABORARIONS INSIDE THE CENTER

A clinical study on oral mucosites prevention in 70 patients submitted to bone

marrow transplantation was carried out as a collaboration of IPEN, FOUSP, Hospital
Sírio Libanês, in São Paulo and Fred Hutchinson Hospital in Seatlle-USA . The laser
system used in this study was developed and customized by one of the companies in
São Carlos (MM Optics). This study was presented as a dissertation at IPEN-SP branch
of CEPOF.
A PhD study (Luciana Almeida Lopes- São Carlos branch) using culture cell
irradiated with low intensity laser is being conducted in São Paulo branch of the Center,
as a collaboration with Prof. Márcia Marques, from School of Dentistry (FOUSP),
facilities that does not exist in São Carlos.
 Project 11- Optics and Photonics in Agriculture
Coordinator- Débora M.B.P. Milori

Researchers
Name
Ladislau Martin Neto
Débora M. B. P. Milori
Pos-Doc
Name
Julieta A. Ferreira

Students

Name PhD (D)

Master of Sciences (M)
Undergraduated Students
(U)
Martha Gonzalés Pérez D
Eduarda R. Carvalho D
Marcilene Ferrari Barriquelo D
Juliana Cristina Barreiro D
José Roberto Garbin D
Marcelo Luis Simões D
Helder V. A. Galeti U
Cristina Iost U

1. Fluorescence Spectroscopy to Value Humification Degree of Organic Matter

(OM)

The humification process increases semiquinone-type free radical concentration (SFRC)

in humus and their quantification by electron spin resonance spectroscopy (ESR) has
been a good indicator of humification degree of soil organic matter. As an alternative to
relatively complex and expensive ESR spectroscopy, fluorescence spectroscopy was
used in this study to evaluate the humification degree of humic acids (HA) extracted
from Brazilian soils under different land use, tillage or cropping system. Three
fluorescence humification indexes of HA were calculated based on work done by
Zsolnay et al. (1999), Kalbitz et al. (1999), and Milori et al. (2002) (Fig.1).

The comparison of the fluorescence and ESR data clearly showed that our proposal to
evaluate humification is efficient. The increasing of resonance with structures related to
humification process (excitation in 465 nm) produces an emission spectra that is
characteristic and selective. Certainly the humification degree has a large interest in
agricultural systems. Also, it is an accessible analysis of soil organic matter stability,
because it measures stable carbon. This can be very useful for studies on carbon
sequestration by soils when associated with CO2 emission.

In conclusion, fluorescence spectroscopy demonstrates to be a useful technique to

evaluate land use and soil management effects on humification degree of soil HA. The
changes in the structure of the soil HA after a short time such as 5 years under new
management practices were easily detected by fluorescence. Compared with ESR,
fluorescence spectroscopy is relatively simple with low cost tools for studying soil
humic substances.

2. Fluorescence Induced by Laser Applied to Study OM of Whole Soil

Some results of the Fluorescence Induced by Laser (FIL) applied to study of organic
matter (OM ) of whole soils are obtained during this year. This new technique has many
advantages. The first one is to make possible this study without fractionating processes
of OM, analyzing the soil near its natural condition. The second advantage is to allow
analyses of insoluble substances of organic matter, such as humina. Third, to value
carbon concentration without produce chemical residues. And finally, this technique
answers even when the soil possess high paramagnetic metal concentrations, where the
studies with magnetic resonance techniques, such as EPR and NMR, become
impracticable. This is the case of the most common soil in Brazil (fig. 2). Moreover, this
technique has a great potential for application in field conditions and can become a
compact sensor.

3. Interactions of Chlorine with Tropical Aquatic Fulvic Acids

Aquatic humic matter in fresh water ecosystems accounts for the major part of the
dissolved organic carbon (DOC) and it is, an important precursor for harmful byproducts
in the drinking water treatment process. We were characterized Aquatic Humic
Substances (AHS), and their components humic acids (HA) and fulvic acids (FA),
extracted from a Brazilian river. The characterization was done for better understanding
of structural composition of these matrices in tropical aquatics environments, that will be
used for latter analyses involving reactions with two types of disinfectant: chlorine and
chlorine dioxide. Techniques used for spectroscopic characterization were: nuclear
magnetic resonance (13C NMR), electron spin resonance (ESR), fluorescence and
absorption of UV-Visible light, diffuse reflectance infrared Fourier transform (DRIFT)
and Fourier Transform Infrared (FTIR). Analysis of the FA samples after reaction with
Cl2 were also made (Fig.3).

4. Photolysis of Aqueous Pesticide Residues in the Presence of Humic Acids

In this work it was investigated the direct and indirect photolisys of pesticide residues, in
aqueous solutions, under UV-visible radiation (280 – 500 nm). The pesticides Atrazine
(ATZ), Imazaquin (IMZ) and Iprodione (IPR) have been used with titanium dioxide
(TiO2) as main photocatalyst and a light source composed of 200W Hg-Xe lamp.
Different kinds of Humic substances were also used as photocatalysts, to know their
behavior in reactions with pesticides and its effect over photocatalysis by TiO2. They
were used standard soil humic acid (SHA) and standard Suwannee River humic acid
(SRHA) in concentrations varying from 1 to 150 mg.L-1. UV-visible absorption
measurements indicated a fast direct photolisys for the three pesticides that, except for
ATZ, reached total degradation over 2 hours of irradiation. When 100 mg.L-1 of TiO2
was additioned, there was observed 30-40% of efficiency increase, in the photolisys rate,
of ATZ and IPR, while IMZ not increase its photolisys rate. Humic acids (HA) addition
in concentrations above 10 mg.L-1 decreased the photolisys rate for all pesticides.
Measurements with spin-trapping Electronic Paramagnetic Resonance (EPR)
spectroscopy, showed that both TiO2 and humic acids (HA) act in a similar way,
photogenerating hydroxyl radicals which would be the responsible for the attack to the
pesticides molecules. However the HA absorbs great amount of radiation below 350 nm
and also react with the photogenerated hydroxyl radical, influencing directly the
efficiency of the photolisys process, turning it strongly dependent of the nature and
concentration of the compounds in the water to be treated.
VII – Phots and graphs

0,45
2,4
I465/I399 R=0.90, P<0.0001
0,40
2,2
A4/A1 R=0.88, P<0.0001
2,0 0,35

1,8
0,30
1,6

A4/A1
I465/I399

0,25
1,4
0,20
1,2

1,0 0,15

0,8
0,10
0,6
0,05
0 2 4 6 8 10 12 14 16 18 20 22 24 26
A465 (a.u.)

Figure 1 - Data showing correlation among fluorescence methods to determine

humification degree. A4/A1 is the humification index proposed by Zsolnay et al. (1999);
I465/I399 is the humification index proposed by Kalbtiz et al. (1999); A465 is the humification
index proposed by Milori et al. (2002).

-4
1.8x10
native forest
no-tillage
conventional tillage
Humification Degree

-4
1.5x10

-4
1.2x10

-5
9.0x10

0.0 - 2.5 2.5 - 5.0 5.0 - 10.0 10.0 - 15.0 15.0 - 20.0

Deep (cm)

Figure 2 – Humification degree in deep valued by Fluorescence Induced by Laser from

three different soil tillage of a Brazilian Savannah. Conventional tillage shows an uniform
behavior and no-tillage shows a meaningful gradient of humification degree. The results
showed through this technique is coherent with other techniques.
 0.18 1-FA
0.16
1 2-FA+Cl2 (t0)
3-FA+Cl2 (t1)
0.14 4-FA+Cl2 (t2)
2
0.12 5-FA+Cl2 (t3)
Abs (λ=280 nm)

3
6-FA+Cl2 (t4)
0.10
4
0.08
5
0.06

0.04

0.02
6
0.00
0.0 0.2 0.4 0.6 0.8 1.0 1.2
Kd

Figure 3 - Chromatograms of FA sample in the presence of Cl2, (t0 = 5 min after the
handling; t1 = 20 min and thus successively in 15min intervals).
Project 11- Optics and Photonics in Agriculture

I – Published Articles

1. ‘Humification degree of soil humic acid determined by fluorescence spectroscopy”,

D. M. B. P. Milori, L. Martin-Neto, C. Bayer, J. Mielniczuk and V. S. Bagnato, Soil
Science, v. 167, nº 11, p. 739-749, 2002.
2. “Tillage and cropping system sffects on soil humic acid characteristics as determined
by electron spin resonance and fluorescence spectroscopies”, C. Bayer, L. Martin-Neto, J.
Mielniczuk, S. C. Saab, D. M. B. P. Milori and V. S. Bagnato, Geoderma 105, 81-92,
2002.
3. ‘Sorption interactions between imazaquin and a humic acid extracted from a typical
Brazilian oxisol”. Ferreira, J. A., Martin Neto, L., Vaz, C. M. P., Regitano, J. B. Journal of
Environmental Quality. , v.31, p.1665 - 1670, 2002
4. Effects from humidity and metal ions on the free radical analysis of peat humus..
Novotny, E. H., Martin Neto, L. Geoderma. Amsterdam: , v.106, p.305 - 317, 2002.
5. .”Stocks and humification degree of organic matter fractions as affected by no-
tillage on a subtropical soil”. Bayer, C., Mielniczuck, J., Martin Neto, L., Ernani, P. R.
Plant and Soil. , v.238, p.133 - 140, 2002.
6. Characterization of humic acids from a brazilian oxisol under different tillage systems
by EPR, 13C NMR, FTIR, fluorescence and UV-VIS spectroscopy”. M. González-Pérez,
L. Martin-Neto, S. C. Saab, E. H. Novotny; D. M. B. P. Milori, V. S. Bagnato, L. A.
Colnago, W. J. Melo, H. Knicker. Accepted on May, 2003 at Geoderma.
7. “EPR characterization of soil humic acids extracted from an oxisol in areas under
sewage sludge applications”. M. González-Pérez, L. Martin-Neto, E. H. Novotny, D.
M. B. P. Milori, R. Berton, W. Bettiol, O. A. Camargo. Submitted to Journal of
Environmental Quality.
8. “Interactions of chlorine with tropical aquatic fulvic acids and formation of
intermediates observed by fluorescence spectroscopy”. Carvalho, E. R.; Martin-Neto,
L.; Milori, D. M. B. P.; Rocha, J. C.; Rosa, A. H. accepted at Journal of Brazilian
Chemistry Society.
II- Published Articles- National Journals

“Fluorescência induzida por laser para análise da matéria orgânica de solos intactos”.
Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto e Cimélio Bayer. Boletim
de Pesquisa e Desenvolvimento - Documento da Série Embrapa n°3, 2003.

III – Participation in National and International Meeting

1. “Changes of molecular size of humic acids determined by controlled pore glass
chromatogrAphy using different eluents”. Martha González-Pérez, Ladislau Martin-
Neto, Débora M. B. P. Milori, Ronaldo Berton, Wagner Bettiol, Otávio A. Camargo. 9th
Nordic IHSS Symposium on Abundance and Functions of Natural Organic Matter
Species in Soil and Water, Mid Sweden University, may 19-21, 2003, Sweden.
2. “Sorption study of ametryne in humic acids from Brazilian soils”. Juliana C.
Barreiro, Julieta A. Ferreira, Ladislau Martin-Neto, Jussara B. Regitano, Milton Duffles
Capelato. 9th Nordic IHSS Symposium on Abundance and Functions of Natural Organic
Matter Species in Soil and Water, Mid Sweden University, may 19-21, 2003, Sweden.
3. “Application of 1H HR/MAS NMR to soil organic matter studies”. M. González-
Pérez, A. G. Ferreira, L. A. Colnago, C. Daolio, R. Berton, W. Bettiol, O. A. Camargo,
L. Martin-Neto. IX Encontro de usuários de Ressonância Magnética Nuclear, Angra dos
Reis, RJ, Brasil, 05 a 09 de maio/2003.
4. Hydrophobic sites in humic acid as detected by ESR of spin-label stearic acid, 13C
NMR of labeled oleic acid and pyrene fluorescence. Ferreira, J A; Martin Neto,
Ladislau; Nascimento, O R; Colnago, L A.. In: 20TH ANNIVERSARY
CONFERENCE HUMIC SUBSTANCES: NATURE'S MOST VERSATILE
MATERIALS, 2002, Boston. Proceedings. Boston: Northeastern University, 2002.
p.388-391.
5. Qualitative changes of soil organic matter detected by 13C NMR and ESR in areas
under carbon sequestration in the Brazil. Martin Neto, Ladislau; Pillon, C N;
Mielniczuck, J. In: 20TH ANNIVERSARY CONFERENCE HUMIC SUBSTANCES:
NATURE'S MOST VERSATILE MATERIALS,2002, Boston. Proceedings. Boston:
Northeastern University, 2002. p. 377-379.
6. Studies of semiquinone free radical by EPR in whole soil, HA, FA and humin. Saab,
S. da C; Martin Neto, Ladislau; Conceição, M. da. In: 20TH ANNIVERSARY
CONFERENCE HUMIC SUBSTANCES: NATURE'S MOST VERSATILE
MATERIALS, 2002, Boston. Proceedings. Boston: Northeastern University, 2002. p.
84-86.
7. “Fotólise de resíduos de pesticidas em solução: influência da presença de
substâncias húmicas”. J. R. Garbin, M. L. Simões, D. M. B. P. Milori, W. T. L. da
Silva, L. Martin-Neto. I Forum das Universidades Públicas Paulistas: Ciência e
Tecnologia em Resíduos, 18-20 de maio de 2003 em São Pedro – SP
8. “Utilização de ressonância paramagnética eletrônica no estudo da influência de
substâncias húmicas sobre a fotodegradação de resíduos de pesticidas em água”. José
Roberto Garbin, Marcelo Luis Simões, Débora Marcondes Bastos Pereira Milori,
Ladislau Martin-Neto. XXVI Encontro Nacional de Física da Matéria Condensada,
Caxambú – Minas Gerais – Brasil, 6-10 de maio de 2003.
9. “Fluorescência induzida por laser aplicada à analises de solos”. Débora M.B. P.
Milori, Ladislau Martin-Neto, Cimélio Bayer, João Mielniczuk. XXVI Encontro
Nacional de Física da Matéria Condensada, Caxambú – Minas Gerais – Brasil, 6-10 de
maio de 2003.
10. “Efeito do ferro na fluorescência induzida por laser da matéria orgânica de solos
intactos”. Cristina Abi Reached Iost, Débora M. B. P. Milori, Ladislau Martin-Neto,
Newton La Scala Júnior, José Marques Júnior. XXVI Encontro Nacional de Física da
Matéria Condensada, Caxambú – Minas Gerais – Brasil, 6-10 de maio de 2003.
11. “Estudos de propriedades magnéticas em substâncias húmicas”. Ronny Rocha
Ribeiro, Antonio José da Costa Filho, Otaciro Rangel Nascimento, Ladislau Martin-
Neto, Claudio José Magon. XXVI Encontro Nacional de Física da Matéria Condensada,
Caxambú – Minas Gerais – Brasil, 6-10 de maio de 2003.
12. “Estudo do efeito da concentração na fluorescência de substâncias húmicas”.
Helder Vinicius Avanço Galeti, Débora M. B. P. Milori, Martha González-Pérez. XXVI
Encontro Nacional de Física da Matéria Condensada, Caxambú – Minas Gerais –
Brasil, 6-10 de maio de 2003.
13. “Fotocatálise de resíduos de pesticidas em solução aquosa na presença de
substâncias húmicas” José Roberto Garbin; Marcelo Luiz Simões; Débora Marcondes
Bastos Pereira Milori; Ladislau Martin Neto. V SICEM, 24 e 25 de outubro de 2002 em
São Carlos – SP
14. “Caracterização de frações cromatográficas de ácidos húmicos extraídos de áreas
sob adição de lodo de esgoto através de fotoluminescência”. M. González-Pérez, D. M.
B. P. Milori, E. H. Novotny, L. Martin-Neto, R. Berton, W. Bettiol, O. A. Camargo
VIII Workshop de Pós-Graduação do Instituto de Química de São Carlos, São
Carlos/SP, Brasil, 27 a 29 de novembro/2002.