Created by GROUP 3

ANACHEM
TEAM
PROJECT

NAMO, EDRIEL LAGTAPON

TAGPUNO, ROCHELLE VIÑARTA
TINGSON, BLESSA MAE ONG
GEOLLEGUE, LAARNIE MAE MAGALLANES
LAVADIA, HANNAH AMANTILLO
LEDESMA, DANIKA ALEC PALACIO

Spectroscopy
 Table of
Content
What is Spectroscopy ?
Who discovered Spectroscopy
Principle of Operation
Basic Parts and Uses
Importance in the Industry
Spectrophotometer
Spectroscopy is the study of the
absorption, emission, and
scattering of electromagnetic
radiation by atoms and molecules
in the gas, liquid, or solid phases.
Although the terms light,
radiation, and electromagnetic
radiation are all interchangeable,
visible electromagnetic radiation
is known as light.

WHAT IS
SPECTROSCOPY ?
 WHO DISCOVERED
SPECTROSCOPY ?
The rainbow contains the spectrum nature of light, yet
it was beyond the ability of early man to comprehend
its importance. Newton did not demonstrate that the
sun's white light could be divided into a continuous
array of hues until 1666. To characterize this
occurrence, Newton used the term "spectrum." A small
aperture was used to define a beam of light, which was
then collidated by a lens, dispersed by a glass prism,
and displayed on a screen. This first spectroscope was
almost modern in design. The science of spectroscopy
began with Newton's examination of light.

Sir Isaac Newton

Generally, Sir Isaac Newton is credited with the discovery
of spectroscopy, but his work wouldn't have been possible
without the discoveries made by others before him.
PRINCIPLES
OF
OPERATION
All spectroscopic techniques work
on the same basic principle: shine a
beam of electromagnetic radiation
onto a sample and study how it
reacts. The response is normally
measured as a function of the
wavelength of the light. A spectrum
is a graph that shows the response
as a function of wavelength.
1. A stable source of radiant energy
(sources of radiation). Basic Parts
2. A transparent container for holding
the sample (sample cell).
3. A device that isolates a restricted
and Uses
Components of typical spectroscopic
region of the spectrum for
measurement (wavelength selector, instruments:
monochromator or grating).
4. A radiation detector, which converts
radiant energy to a usable electrical
signal.
5. A signal processor and readout,
which displays the transduced signal.
Importance in
the Industry

It can be used to comprehend
chemical reactions such
heterogeneous catalysis and
hydrothermal synthesis of
industrial materials, as well as
offer information on bio-
remediation processes and
examine minute minerals
returned from space missions.
Spectroscopy procedures are essential
in today's analytical laboratories in
industry and research. Spectroscopy
applications are routinely utilized in
many areas of practically all
manufacturing businesses and in
almost every research laboratory for
quality control, purity testing, structural
studies, reaction process monitoring,
materials testing, and much more.
Spectroscopy approaches can
be used in the following areas:
1. Structure analysis of chemical
compounds
2. Incoming goods and process control
in the pharmaceutical industry
3. Detection of contaminant objects and
quality control in the food industry
4. Identification of substances in
toxicology and forensics
SPECTROPHOTOMETER

SPECTROPHOTOMETER
3D MODEL DESIGN
by: DANIKA LEDESMA
An instrument which can measure

the absorbance of a sample at any

below:
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wavelength. Used to identify https://www.tink
unknown substances and determine ercad.com/things
their concentration and measures /iQGz1XR2QCj
electromagnetic absorption.
 PRINCIPLES OF
OPERATION
1. A prism (or) grating is employed in the
spectrophotometer to split the incident beam
into multiple wavelengths.
2. Waves of specific wavelengths can be
manipulated to fall on the test solution using
appropriate techniques. The wavelengths of
incoming light can range from 1 to 2
nanometers.
3. The spectrophotometer is used to determine a
compound's absorption spectrum, or the
amount of light absorbed by a solution at each
wavelength.
The principle of operation of
the spectrophotometer is
based on Beer-Lambert's law
which states that the amount
of light absorbed by a color
solution is directly
proportional to the solution's
concentration and the length
of a light path through the
solution.
The spectrophotometer
technique is used to determine
the intensity of light as a function
of wavelength. This is
accomplished by diffracting the
light beam into a spectrum of
wavelengths, detecting the
intensities with a charge-coupled
device, and graphing the results
on the detector and subsequently
on the display device.
 Five Basic Parts of
Spectrophotometer:

1. Light Source - supply electromagnetic energy.

2. Monochromator - isolate the object of interest and eliminate the
unwanted second order radiation.
3. Sample Compartment - to accommodate the sample solution.
4. Detector - receive the transmitted light and convert it to electrical signal.
5. Digital or analogue display- to indicate absorbance or transmittance
 A spectrophotometer is used in many areas of science including
microbiology, biochemistry, forensics, physics, and medical health.
You can use it to measure certain ingredients in a drug to make
sure it is effective and safe for consumers. You can measure
bacterial growth, or diagnose a patient based on how much uric
acid is present in their urine. Even non-scientists use
spectrophotometers. Wine-makers, for example, use them to
determine how much malic acid (reducing sugars) a particular wine
has in it.

Scientists can also use the

spectrophotometer to see how a reaction
has progressed. Let's say you're studying
iron deficiency, a condition that affects
over 2 billion people. Antioxidants,
vitamins, and other agents are known to
help in iron deficiency, but they don't
have a single specific chemical make-up,
and so they can't be directly measured.
However, they are known to reduce ferric
iron into ferrous iron, a more valuable

Uses of form to the human body.

Spectrophotometer
With the use of such a device, the person using a
spectrophotometer can easily acquire spectra of
shining white light on a given sample thereby
measuring the light that is returned from the
sample.
The device is used in many different fields,
especially in the production facilities and scientific
laboratories. In a production facility, a
spectrophotometer is used to check for the quality
of product such as in clothing and emission of light
during the manufacture of LED light, to name a few.
In analytical laboratories, a spectrophotometer is
used for the identification and quantifying
microscopic samples such as matching colors,
kinetics, qualifying gems and minerals, determining
the color of paint or ink, and so on.

Why is the
spectrophotometer
important?
 REFERENCES
https://chem.libretexts.org/Bookshelves/Physical_and_Theoretical_Che
mistry_Textbook_Maps/Book%3A_Quantum_States_of_Atoms_and_Mole
cules_(Zielinksi_et_al)/01%3A_Spectroscopy.
https://web.mit.edu/spectroscopy/history/history-classical.html.
http://photobiology.info/Nonell_Viappiani.html#:~:text=The%20basic%2
0principle%20shared%20by,a%20function%20of%20radiation%20wavel
ength.
http://web.iyte.edu.tr/~serifeyalcin/lectures/chem305/cn_2.pdf
https://www.bionity.com/en/focus/5/spectroscopy/19/spectroscopy-
applications-in-industry-and-research.html.
Source, D. (2022). Spectroscopy for industry | Diamond Light Source - -
Diamond Light Source. Diamond.ac.uk. Retrieved 7 May 2022
https://www.diamond.ac.uk/industry/Industry-News/Latest-
News/Synchrotron-Industry-News-Focus-
Spectroscopy.html#:~:text=X%2Dray%20absorption%20spectroscopy%2
0is,fluids%2C%20and%20complex%20inhomogeneous%20materials.
 REFERENCES
Aryal, S. (2022). Spectrophotometer- Principle, Instrumentation,
Applications. Retrieved 20 May 2022, from
https://microbenotes.com/spectrophotometer-principle-
instrumentation-applications/#principle-of-spectrophotometer
BATRA, S. (2022). SPECTROPHOTOMETER - Principle, Components,
Working & Application | BIOCHEMISTRY PRACTICALS. Retrieved 20 May
2022, from https://paramedicsworld.com/biochemistry-
practicals/demonstration-of-spectrophotometer-principle-components-
working-applications/medical-paramedical-studynotes