Protocol report

Presto_MVP_200uL_VX2_Mmtm_V2_1
2022-06-27 12:26:38+08:00 1/6

General info

Protocol information
Protocol name Presto_MVP_200uL_VX2_Mmtm_V2_1
Modified by hannah.pezzi
Kit name MagMAX MVPI and MVP II
Description MagMAX MVP for Viral RNA extraction from 400uL Liquid sample

Run information
Execution started 2022-06-27 12:06:53+08:00
Executing user ameer.affandi
Total warnings 0
Total errors 0

Laboratory information
Laboratory name
Address
Phone
E-Mail

Instrument parameters
Instrument name 713-8G0095
Instrument type KingFisher Presto
Firmware version 1.2.3
Instrument serial number 713-8G0095

Software parameters
Software version BindIt Software 4.0 for KingFisher Instruments

Run log
2022-06-27 12:06:53 Presto_MVP_200uL_VX2_Mmtm_V2_1: started
2022-06-27 12:06:53 Temperature: 27 / 31 °C
2022-06-27 12:06:56 Pick-Up: 'Tip1' load.
2022-06-27 12:06:56 Load plate: started
2022-06-27 12:07:15 Load plate: started
2022-06-27 12:07:23 Temperature: 30 / 31 °C
2022-06-27 12:07:27 RotateDial: started
2022-06-27 12:07:29 Change plate: started
2022-06-27 12:07:29 Remove plate: started
2022-06-27 12:07:29 Load plate: started
2022-06-27 12:07:29 Extraction0: started
2022-06-27 12:07:29 Change plate: started
2022-06-27 12:07:29 Remove plate: started
2022-06-27 12:07:29 Load plate: started
2022-06-27 12:07:53 Temperature: 39 / 32 °C
2022-06-27 12:07:59 Change plate: started
2022-06-27 12:07:59 Remove plate: started
2022-06-27 12:07:59 Load plate: started
Protocol report
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2022-06-27 12:07:59 Extraction: started

2022-06-27 12:07:59 Change plate: started
2022-06-27 12:07:59 Remove plate: started
2022-06-27 12:07:59 Load plate: started
2022-06-27 12:08:23 Temperature: 47 / 33 °C
2022-06-27 12:08:53 Temperature: 54 / 33 °C
2022-06-27 12:09:23 Temperature: 60 / 34 °C
2022-06-27 12:09:53 Temperature: 67 / 34 °C
2022-06-27 12:10:23 Temperature: 69 / 35 °C
2022-06-27 12:10:53 Temperature: 69 / 34 °C
2022-06-27 12:11:23 Temperature: 70 / 34 °C
2022-06-27 12:11:53 Temperature: 70 / 34 °C
2022-06-27 12:12:23 Temperature: 70 / 34 °C
2022-06-27 12:12:53 Temperature: 70 / 33 °C
2022-06-27 12:13:23 Temperature: 70 / 33 °C
2022-06-27 12:13:53 Temperature: 67 / 33 °C
2022-06-27 12:14:23 Temperature: 64 / 32 °C
2022-06-27 12:14:38 Wash1: started
2022-06-27 12:14:40 Change plate: started
2022-06-27 12:14:40 Remove plate: started
2022-06-27 12:14:40 Load plate: started
2022-06-27 12:14:53 Temperature: 61 / 32 °C
2022-06-27 12:15:23 Temperature: 67 / 32 °C
2022-06-27 12:15:53 Temperature: 70 / 33 °C
2022-06-27 12:16:23 Temperature: 70 / 33 °C
2022-06-27 12:16:53 Temperature: 70 / 33 °C
2022-06-27 12:17:15 Wash2: started
2022-06-27 12:17:17 Change plate: started
2022-06-27 12:17:17 Remove plate: started
2022-06-27 12:17:17 Load plate: started
2022-06-27 12:17:23 Temperature: 70 / 33 °C
2022-06-27 12:17:53 Temperature: 70 / 33 °C
2022-06-27 12:17:58 Elution1: started
2022-06-27 12:18:00 Change plate: started
2022-06-27 12:18:00 Remove plate: started
2022-06-27 12:18:00 Load plate: started
2022-06-27 12:18:23 Temperature: 70 / 33 °C
2022-06-27 12:18:53 Temperature: 69 / 33 °C
2022-06-27 12:19:23 Temperature: 69 / 33 °C
2022-06-27 12:19:53 Temperature: 70 / 33 °C
2022-06-27 12:20:23 Temperature: 70 / 33 °C
2022-06-27 12:20:53 Temperature: 70 / 33 °C
2022-06-27 12:21:23 Temperature: 70 / 33 °C
2022-06-27 12:21:54 Temperature: 70 / 33 °C
2022-06-27 12:22:24 Temperature: 70 / 33 °C
2022-06-27 12:22:54 Temperature: 70 / 33 °C
2022-06-27 12:23:21 Elution2: started
2022-06-27 12:23:24 Temperature: 71 / 33 °C
2022-06-27 12:23:54 Temperature: 67 / 32 °C
2022-06-27 12:24:24 Temperature: 64 / 32 °C
2022-06-27 12:24:54 Temperature: 61 / 32 °C
2022-06-27 12:25:24 Temperature: 58 / 31 °C
2022-06-27 12:25:31 Leave: 'Tip1' leave.
2022-06-27 12:25:33 Remove plate: started
2022-06-27 12:25:47 Remove plate: started
2022-06-27 12:25:54 Temperature: 55 / 31 °C
Protocol report
Presto_MVP_200uL_VX2_Mmtm_V2_1
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2022-06-27 12:26:24 Temperature: 53 / 31 °C

2022-06-27 12:26:38 Presto_MVP_200uL_VX2_Mmtm_V2_1: completed
Protocol report
Presto_MVP_200uL_VX2_Mmtm_V2_1
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Reagent info

Sample 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Binding/Bead Mix 275 - Reagent
Sample 200 - Reagent
PK/MS2 10 - Reagent

Wash 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Wash Soln 500 - Reagent

80p_EtOH 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
80% Ethanol 1000 - Reagent

Elution 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Elution Solution 100 - Reagent

TipComb 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
- - - -

Empty 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
NoReagent 50 - Reagent

Dispensed reagents

The protocol does not contain dispensed reagents

Protocol report
Presto_MVP_200uL_VX2_Mmtm_V2_1
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Steps data

Tip1 96 DW tip comb

Pick-Up TipComb

RotateDial Empty
Beginning of step Precollect No
Release beads No
Mixing [none]
Heating during mixing No
End of step Postmix No
Collect beads No

Extraction0 Sample
Beginning of step Precollect No
Release beads No
Mixing [none]
Heating during mixing No
End of step Postmix No
Collect beads No

Extraction Sample
Beginning of step Precollect No
Release beads Yes
Mixing / heating: Mixing time, speed 00:05:00, Fast
Heating temperature [°C] 70
Preheat Yes
End of step Postmix No
Collect count 5
Collect time [s] 0

Wash1 Wash
Beginning of step Precollect No
Release time, speed 00:00:10, Bottom mix
Mixing / heating: Shake 1 time, speed 00:00:10, Bottom mix
Shake 2 time, speed 00:00:10, Fast
Loop count 2
Heating during mixing No
End of step Postmix No
Collect count 5
Collect time [s] 1

Wash2 80p_EtOH
Beginning of step Precollect No
Release beads No
Mixing / heating: Mixing time, speed 00:00:20, Medium
Heating during mixing No
End of step Postmix No
Collect beads No
Protocol report
Presto_MVP_200uL_VX2_Mmtm_V2_1
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Elution1 Elution
Beginning of step Precollect No
Release beads Yes
Mixing / heating: Shake 1 time, speed 00:00:15, Bottom mix
Shake 2 time, speed 00:00:45, Medium
Loop count 5
Heating temperature [°C] 70
Preheat Yes
End of step Postmix No
Collect beads No

Elution2 Elution
Beginning of step Precollect Yes
Release beads No
Mixing / heating: Mixing time, speed 00:01:00, Slow
Heating during mixing No
End of step Postmix No
Collect count 2
Collect time [s] 15

Leave TipComb