GI Organisms Spread Sheet

Gram Negative Rods (All Gamma Hemolytic)

Organism Lactose (MAC) H2S (HE) HE Carb. Motility Urea Indole Citrate
positive/inhibited No
Escherichia coli positive negative growth positive (very) negative positive Growth/Negative
Salmonella choleraesuis negative positive negative positive (very) negative negative positive
No
Shigella boydii negative negative negative negative negative negative Growth/Negative
Enterobacter aerogenes positive negative positive positive negative negative positive
Providencia alcalifaciens negative negative negative positive negative positive positive
Klebsiella pneumoniae positive negative positive negative positive negative positive
No
Proteus mirabilis negative positive negative positive positive negative Growth/Negative

Gram Positive Organisms

Organism Shape (cell) Hemolysis (BAP) Catalase (TSA) Coagulase Bile Growth (BEA) Esculin (BEA) Motility
Staphylococcus aureus cocci beta positive positive growth negative n/a
rods(spores-
Bacillus cereus maybe) gamma postive n/a growth negative positive (very)
Listeria monocytogenes coccobacilli gamma positive n/a growth positive n/a
rods(spores-
Bacillus subtilis maybe) beta positive n/a growth positive positive (very)
Streptococcus pyogenes cocci beta negative n/a no growth n/a n/a
Enterococcus faecalis cocci gamma negative n/a growth positive n/a
Streptococcus agalactiae cocci beta negative n/a no growth n/a n/a
Staphylococcus epidermidis cocci gamma positive negative growth negative n/a

n/a = not tested or not applicable