January 27, 2022

To: Ms. Robin Xu

Assistant Editor
Water MDPI

Dear Ms. Xu

We would like to present the modified version of the paper entitled “Removal of
nutrients and pesticides from agricultural run-off using microalgae and
cyanobacteria” according to the different comments suggested by reviewers.
You can find in the second version (V2) of the summited paper all the modifications
highlighted in yellow (reviewer 1), green (reviewer 2), and cyan (reviewer 3)

I look forward to your positive response. Please contact me if you need any further
information. With kindest regards,

Prof. Antonio ZUORRO, PhD

Researcher & Project Manager
Department of Chemical Engineering, Materials & Environment
Sapienza - University of Rome, 00184 Rome, Italy
+39 0644585401 | +39 3283731079 |
antonio.zuorro@uniroma1.it
Section Editor-in-Chief of Energies
Editorial Board Member of Water

Università degli Studi di Roma “La Sapienza”

Dipartimento Ingegneria Chimica Materiali Ambiente
CF 80209930587 PI 02133771002
Via Eudossiana 18, 00184 ROMA
T (+39) 06 44585600 F (+39) 06 44585451
http://ingchim.ing.uniroma1.it/
 Pag 2

Reviewer 2 (green)

# Comment Action section

1 The collection of wastewaters has not specified Section Line 91-93
the location and time, before, star or during the added
agricultural season. The methods of collection.
2 It is not clear why 3 strains Hapalosyphon Section Line 101-105
sp (HAPA_UFPS002), Chlorella sp added
(CHLO_UFPS010), and Scenedesmus 98 sp
(SCEN_UFPS015) were selected for this
study.
3 The insecticide chlorpyrifos was selected to evaluate Idea Line 44-46
the absorption capacity of microalgae strains. Why? added
Please explain in the introduction part instead of in
discussion only.
4 Table 1 can be omitted; just table 2 is enough Tables New table 1
merged
5 In Table 2, please specify the threshold of Tables New table 1
physicochemical criteria that can be accepted by upgraded
Colombian regulations (Resolution 631 - 2015).
6 In Figure 1, the Agricultural run-off ratio of 10% Idea Line 138-143
(v/V) gives the lowest biomass results, below added
0.5g/L, why?
7 Please write the full UFPS (line 99). Full name Lines 104-105
added
8 We do not understand this “The removal Idea Lines 180-186
efficiency of chlorpyrifos was determined in the added
experiments with the highest biomass
concentration” pp. 165-166. Please specify the
conditions and parameters of the experiment,
using 3 strains of microalgae to remove
Chlorpyrifos. Because this is an important
experiment in this study