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Mahesha H B
University of Mysore
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1
Observations and Calculations
Volume of Volume of Concentration of Volume of
standard BSA distilled water Protein (mg) Biuret reagent A540
(ml) (ml) (ml) Incubate
0.0 1.0 00 3 At 37ºC 0.00
0.2 0.8 1 3 for
0.4 0.6 2 3 10
0.6 0.4 3 3 Min
0.8 0.2 4 3 & Cool
1.0 0.0 5 3
1.0 UK 0.0 To be estimated 3
References
1. A Manual of Laboratoory Techniques, 1983. Editors: Raghuramulu N., Nair, M K and
Kalyanasundaram, S., National Institure of Nutrition, Hyderabad, Inndia.
2. Plummer D.T., 1988. An introduction Practical Biochemistry, 3rd
r
edition, Tata Mc
Graw-Hill publishing Company,
C New Delhi, pp 159.
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