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  • HPLC Peak Area Vs Concentration Graphs

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    Cheyanne Morris
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    The document contains data from calibration curves of three compounds: N-Acetyl-p-aminophenol, acetylsalicylic acid, and caffeine. Peak area measurements were taken at varying concentrations of each compound, and linear regression was used to generate calibration curves with R2 values close to 1. These curves allow converting between concentration and peak area for quantitative analysis. The compounds were then analyzed in a sample of Excedrin and amounts determined with percent errors all under 12%.

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    HPLC Peak Area vs Concentration Graphs

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    The document contains data from calibration curves of three compounds: N-Acetyl-p-aminophenol, acetylsalicylic acid, and caffeine. Peak area measurements were taken at varying concentrations of each compound, and linear regression was used to generate calibration curves with R2 values close to 1. These curves allow converting between concentration and peak area for quantitative analysis. The compounds were then analyzed in a sample of Excedrin and amounts determined with percent errors all under 12%.

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    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
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    75 views7 pages

    HPLC Peak Area Vs Concentration Graphs

    Uploaded by

    Cheyanne Morris
    The document contains data from calibration curves of three compounds: N-Acetyl-p-aminophenol, acetylsalicylic acid, and caffeine. Peak area measurements were taken at varying concentrations of each compound, and linear regression was used to generate calibration curves with R2 values close to 1. These curves allow converting between concentration and peak area for quantitative analysis. The compounds were then analyzed in a sample of Excedrin and amounts determined with percent errors all under 12%.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 7

    N-Acetyl-p-Aminophenol Acetylsalicylic acid Caffeine

    Concentration (ppm) peak area Concentratpeak area Concentraipeak area


    100 29134452 100 1726139 20 2625824
    50 14549987 50 803604 10 1319571
    80 23414210 80 1337048 16 2110360
    120 34992941 120 2074830 24 3155825
    160 46325770 160 2817698 32 4209159
    200 56777050 200 3545533 40 5192713

    Peak area vs. Concentration of N-Acetyl-p-Aminophenol


    60000000
    f(x) = 282156.4806271 x + 810551.459126547
    50000000 R² = 0.99948866040049

    40000000
    Peak Area

    30000000

    20000000

    10000000

    0
    40 60 80 100 120 140 160 180 200 220
    Concentration (ppm)

    Peak Area vs. Concentration of Caffeine


    6000000

    5000000 f(x) = 129508.948488242 x + 37196.8857782753


    R² = 0.99983411999976
    4000000
    Peak Area

    3000000

    2000000

    1000000

    0
    5 10 15 20 25 30 35
    Concentration (ppm)
    23.09931 0.577483 Actual Weight (g)
    3028766 Actual Concentraion (ppm)

    Peak Area vs. Concentration of Acetylsalicylic


    acid
    4000000
    3500000
    f(x) = 18315.9014557671 x − 116573.005599104
    3000000 R² = 0.999932708449474
    2500000
    Peak area

    2000000
    1500000
    1000000
    500000
    0
    40 60 80 100 120 140 160 180 200 220
    Concentration (ppm)

    tration of Caffeine
    N-Acetyl-p-aminophenol (M)
    Acetylsalicylic Acid (M)
    857782753 Caffeine (M)

    25 30 35 40 45
    ation (ppm)
    Acetylsalicylic Acid N-Acetyl-p-aminophenol Caffeine Excedrin
    0.0495 0.0493 0.0100 0.6731
    198 197 40

    Acetylsalicylic Acid (ppm) N-Acetyl-p-aminophenol (ppm) Caffeine (ppm)


    198 197 40
    158 158 32
    119 118 24
    99 98.6 20
    79.2 78.9 16
    49.5 49.3 10
    N-Acetyl-p-Aminophenol N-Acetyl-p-Aminophenol Caffeine
    Concentration (ppm) peak area Concentration (ppm) peak area Concentraiton (ppm) peak area
    98.6 29134452 98.6 29134452 20 2625824
    49.3 14549987 49.3 14549987 10 1319571
    78.9 23414210 78.9 23414210 16 2110360
    118 34992941 118 34992941 24 3155825
    158 46325770 158 46325770 32 4209159
    197 56777050 197 56777050 40 5192713

    Acetyls
    n-acetyl p aminophenol 60000000

    60000000 f(x) = 286313.474


    50000000 R² = 0.999513949
    f(x) = 286313.474851005 x + 805373.383211087
    50000000 R² = 0.999513949054155
    40000000

    Peak Area
    40000000
    30000000
    Peak Area

    30000000
    20000000

    20000000
    10000000

    10000000
    0
    40 60 80 1
    0
    40 60 80 100 120 140 160 180 200 220 Concentration
    Concentration of N-Acetyl-p-Aminophenol (ppm)
    print out new items for notebook
    add new graphs in

    Acetylsalicylic acid
    000000
    f(x) = 286313.474851005 x + 805373.383211087
    000000 R² = 0.999513949054155

    000000

    000000

    000000

    000000

    0
    40 60 80 100 120 140 160 180 200 220
    Concentration of Acetylsalicylic Acid (ppm)
    Expected Amount in Excerdin (mg)
    N-Acetyl-p-aminophenol 231 250
    Acetylsalicylic Acid 264 250
    Caffeine 58 65

    Conceltration (M) Actual Amount in Excerdin (mg)


    N-Acetyl-p-aminophenol 91.0000 228
    Acetylsalicylic Acid 105.00 262
    Caffeine 23.0993 58
    Percent Error (%)
    7.6
    5.6
    10

    Expected Amount in Excerdin (mg) Percent Error (%)


    250 8.8
    250 4.8
    65 11

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