PAGE NO.

:
Experiment No:: 01 DATE

Aim To perfom ad idenifs the qualitativee

anaysis oF given_catbahl.fate Sample
Requiremeais --
1Aepratus TeSt tubeS tes tube holder, test Aube
Stand beaker, measurinq cqlinder burnerela-

2themicals -Napthol_conc. HaSO fehling's Salution

AQ0d 6,Benedicts reaqent .tommer'S
reaqent bacfo ed's reaqent conC HeL etc

Test observakions Ioferenee

LMolish Reaqeni
Aqeous or aicnholic SolutionNioletting at Catbohydrate
ot Substance t to1 AC. Salution the junctionpresent.
Of ANapthol Shake t conc H2904of iuwo liquids
alonq he Side Of the tube

2 Solubiliy Manosaacharides

Substance t atet Soluble 4 digaccharides

3Feblinq'S lest
2m o ffehling's SOlution A+ ellow otbrickReducinq
2m0 fehling's Solution tred pot Sugars absent
2m of Suqat solution Sailoabsent
 PAGE NO.:
DATE

4 benedictls testt
5mA o benedictH reaqeni t Green uellon Reducing
3miof Sugar Solution_boil o r ted pPt Sugars absent
Sor 2 minuies coolL absent

5 Tommer's te
2m of tommers reaqent+Neloco or red Reducing
am oP Sugar Solation hoil_ pPL abSent Sugarsabsent
Ror 2minutes Cool.

Conirnaiory egt or Sucroge

nNersioD 5m.of Lest Solution + 6 drops

jest
of Conc hudrochlarie acid boil for 3 miouAes
Cool Ndet the ap water.then 0eutrQlise
oith CauStic Sodq 0oL oliocainq teskS fo
this Solutio) are posi4ive
i febinqs test
Genediclg est
I Tomamers tesi

Test abgeryaHlon. loereOce

fehlinqs les
2m-0f fehling's Soluhion yeilon at led Aeducionq
2mof Rehlingis Solukion6 pet piesentSugas Present
2m.al Sugar Solalianboi
 PAGE NO.: 3

DATE

2 Benedictls test
5m of benedict's teagent t Qreen iellou ReducingH
m of Sugar Solution boilfotlor red potSugars
2 minutes 0001 presentL presentt

3Tommers test
2.mo tomners reagenOA +dm Uellou OrReduciag
a Sugat Solution boil Ror 2 ted ppA SUgars
mioute9 Cool present presenE

Result = The aiyen_ONKNoON Sanple 0as

ound a be Sucrose

Referenoe
S R Kaie, R-A. Kale: A practicalbaok of
biochemsHY and clinical pauhology
Nifal pCLqe No. 4 t66 Nifalipraka9ha)
 PAGE NO.: 4

xperiment No 2 DATE

Aim 10 Perfaim anelldentify the quaiative

analusis 0f iven carbohydrale Sample

Reqtuitements
1APpatatus Test tubes. Lesl tube stand. test
tube holder, beaker meaguri0q
Cylinder burer etc
2 chemicals napBhol. Conc. HS04febtinqs
Solutian benedictis reaqent tomme.
rS reagent lodine Solution, NaoH,
Starch
SAmple-A
Test obaeryotionTnference

| Malishlesi
Ageous or alconolic solution of Vialel ring carbohydrak
SubSiance t o 1. AIC.Solution of atthe4nd present
AnaphholShaKe t conc. H2SoLio0 aF tuoo
along the gide of the lube liquids

Solubilliy
SUbstance +water Insoluble polysacch
-tid-es

3 Tadine Lest
SUSpenSionor Solution of a) biye Viole Statch iS
Dolysacbaride i-2 droPS tcolout present
o 1odlinesolutioD develops
 PAGE NO.: 5

DATE

Onfitmatory tesis for

9tarch
a) ami of Socdium hyclroxide No blue Starchiss
+Starcb. jely ike masS formedcolout Confiimed
+ lodine SolutionD
b Above SoluioN4NaoH
Solution

Result The QIyen uOKDOCOD Sample was founa

tobe Starch

Reference
R. KaleR.A. Kqie A practical book op
Biochemnigts a0d clioica pathoIOqs Paqe NO.
y-6 Nitaii prakashan
 PAGE NO.: 6

Experiment No 03 DATE

Aim To petform end IdenHiy tbe 9ualitative

anolysis of Hhe given carbohydrate Sample

Requiremeots
A) ApotauSTest Hubes. tesl jube holder. test tube
SBand. beaketburner etHc
B)chemiais molisb reaqent. febiDq Solution A and B,
benedicts reaqe0t tomners reagent
barfoed's reagent Seliwanoffls teagent
onc. H c

Test observation inference

I Molish Test
Aqeous or alcaholic solution of Vialet rinq Carbohydrat
substance io1 AIC. Solution of at inejunct: present
A Oapthal ShaKe t conc. HaSo04 ion of iwo
along the Side of he iube iquidS9

Solubility Test MOno and

Sotuble disac harides
SubStance +watet
3 fehings tes
2mof fehlingS 901atlon A.and uellou Ot Reducing
62m of Sagar51otution boil brticK Ied5ugars
DALDLeSeni preSeni
 PAGE NO.: 7
DATE

4 benedicts Test
mof benedicils teageni+ greenellou Reducing
md o Sugar Solutioo boil OF ted pp Sugars
fOr 2minutes_Cool presentt presen

5 Tommer'g Test
2m 0f tommeris teagentt yello Or Reddcio0q
m0P Sugat Solutian bailted ppl Sgars
Aor 2 minates Cool. presendt presenat

6Garfoed's Test
2m.oR Lest Solutiodta.m 8rick red pPiMonoSac-
of barfoed's reagent boil an lat thebottom -harides
waterbath Of the test present
ube present

seliaanof's est
3m of Seliwanoff's teaqent Red pP KetoseS
im of Sugar Salution preseni ike ftuctos=
boil or 2 minutes Sucrase
present

8 Rapid furfurai Test DeeP purpe KetoseS

Colaur pesenL liKe fuctase
SucroSe
present
 PAGE NO.: 3

DATE

Result The given UOKnOwn Samplee

wasfaund to be fruchOSe

Reference
S R. Rae. R R.HaieA practical bao
bf biochemisiry and clinical patho la qy
pageNo - 6 Nitali piaKashan.
 PAGE NO.: 9
DATE
EXperiment No::04

Aim To Perform 9Ad ldentifs the Qualiia tive

gnalysis of give0 Catbohudate Sample
Requitemenis
A BpeaHUS Test tube jest tuhe holder, test tube.
Sland beaker, measuring culinder.burner etc.
B)chemimls molish_reaqent fehlinq'S SolutioN Aand B,
benedicl!S reaqent lommers_reaqentt
har foed's_reagent Phenl hydrazine hsdm
-chloride, SodiuM acetate etc.

Test obserYationTDference

Molisb Test
Ageous or aicoholic Solution of Violet ring carbohydrate
SubsBance t lo1 AlC. Solulion of_at the undionpresent
A Naptholshake t Conc. HaS4O Huno liquids
alonqthe Side of the tube
2 Solubilits Test
Substance t uater Salubie MonoSa.ccharide=
anddisaccha1rt

lehinqs Tesl
20ofPehlinqts 3olution(AtB)+lellow atbtick Reducing Guqar
2mOF Sugar 5olulionboil- ed ppt plesent l:9 Present
 PAGE NO.: 10

DATE

4)Benediqls TesL
smof benedicts teagent tGreen ello Reducing
3 mo Sugar Salution boi for lar red DPASugars
2minuteS Coa present present

5) Tommer.g Test
2m af tommerg teagent +Nelloo or ted Reducing
am oP Sugar Solution boilpet presentSugars
Ror 2 minuteS CO0 preseOt_

6 Barfoed's Test
m of tesi Solution t 20 oP 8rick ted pPA MonoSa.cchaticde
barfoed S reagent bail an Laaier absent absent

bath

asozoneTest
0-2 gm of Suqar _0:4 gmg Thin Smal
o phenyl hudrazine hsdrochl-lneedle ShqpedLackosqzone
oride t o.6 gms. of SodiuM_ erystais appearli.e lactose
acetate t umd-watet heat on like bail ofpresent
a watE bath {or 20 minuBes. prickles
Cao gnd aloun cuSallisatio n.
observe cryskais undet
microScope.

8 Con fuMOHOTr esA O lactoSe

Teal Solution+ Ammonicalsilvernitt siver LactoSe
ate SoC NHLOHAAqNoEfeess + Mittor Confir med
NOOHD watm
 PAGE NO.:
DATE

Resault Thegiven oDknOLDn Sampe uas

foundtobe Lactoses

Reperence 5R aue R:R. Kqie A_practica

book o biachemists and clinica
Pathal0gypage No to Nitali
prakashao
 PAGE NO.: 12

Experiment No 05 DATE

Aim To Perform Ond IdentiAs the qualitative

analysis of given Carbohydate Sample
Requirenmenis:

A APpataBus Test iubes. lest \ube Stand, test tube

holdet beaker measuinq Cyindet,burnerec.

8) chemicals molish reagent COnc. H2904 fehiing's

Solation A+8, ben.edicis reagenttommers
reaqent bacfoed's reaqeOt phens|_
hydrazine hådrochioride Sodium acelate

Test obser vationtoerence

) Molisb 1est
AqenuS or alcoholic SolutioD of violei tinq Catbohuclrate
Substance t 101. AIC 5oluAtion of lat the Junc present
A Napthol shake t Conc. H2S04 -ion of tu0o
aionqthe Side of the ube liquids

2) aalGh Solubiliis Test- Monosacharide

SUbSBance toatet Saluble and Poiysachaide

3) Febliaqts Test
2m 0 Pehling's SoluHoo A +2m Yellod or Reducinq
Ofehlinqi9 Salutio08 2moP bick ed egi5ugars
Sagar 9olitiaD bail DSen PLesent
 PAGE NO.: 13

DATE

LD Benedicls test Green yellom Reducinq

Sm of benediclS reaqent t3m or red PP Sugars

Of Sugar Solutlon bailfor 2mi0 CoolpreseOA present

5 Tommer's Test Yello or red [Reducing

Amof bmmers renqent t3.miolpp presentSugars-
Present
Sugar solution boil for 2mni0

6) 8arfoeds TestL
Brick red MOnOSa.ccharide-
2mol test Salution+2mt of_
Bar foed'g reaqent boilonwatetbdih_pPA absent absent

DOSazone Tegtt
0.2 9ms ol Sugat0-4 qmsof pheny| Plate lihe maltoSazone
hydrazine hydrochloride t 0-6g.ms cStals appear lie maltose
o Sadim acetake tmtuwater.heat like SuOElOLuerpresent
on a water bath for 20.min Coalcnd
allo ctusStdlisatiog obserye crystdls-
onder mictoscapee
Resalt The qiven unKnoon SampiewaS ound to be
MaItOSes

Relerence
S Akale,RA kale, A PractLCal ddak Of
biochenisty and clinc Daiholoqu paqe NO
S Nital prakashon
 PAGE NO.: 14

Experiment No: o6 DATE

Aim To perfotm and identify the Qualitatire

anaysis af given Carbohydrate Sample

Requitement
0)Aeparatug Test ubes test tube holdet, Aest_tlbe
Stand,benker.meaSuring cylinder etc
8 chemials molish reaqent, Conc:H2S04, fehlinq's Saluti
O0 and . benedicig reagenttommers
teaqent bar foedis reaqent Seliug.noff's
leaqenA coc. Hel SDdiumhudoride methqlene
bluePoBassium ferticuanide pictic acid elc

Test obseryation oference

) Molisch Test
Ageaus o aicanolic SalutianofSubsita- |Violet ting Catbohudate
nce 10 AlC Solution of ANaphol at the. pIesent
ShaKe t cone. Ha1S04 alonq the iunction of
Side o f the lest uhe Huo liquids

2 Solubiliy est
SubStance t watep_ Soluble MOnaSacchatides
anddisacha.
tides

3 ehinqs Test
2m cof lehlinqs Solutuan At- a
 PAGE NO.: 15

DATE

2m of lehlinqS Solution GtNeliotoar brics Reducinq

2mlo Suqar SOlution boil ted pp Sugars
Present presen

4)
Benedicis Test
5m aP benedicls teaqent t 3m Gtee0Yello Reducinq
af Sugar Solution bail fot 2 or Led ppt Sugars
presentL_ Present
minuteS COo

5 Tommer s eSL
2.m aP tommer9 teagent t3ml yeliouo or ted Reducinq
of Sugar Solution boil for 2minuie Ppt prese0tSugars
Coo present

6 Barfoed's Test
2m a test Solutio.n4 2m0 Brick red PPA Monasa.chatide
arfoeds reaqe0t boil o0 water present present
bath
) SeiLwanoff's est
3m oP Selioanoff's reaqent tRed pet Keloses like
Hm of Sugat 501utio.n boli fbrabsent Kructose Sucrs
2 minutes absent

2) Rapid farfura Test

2 0 o f Suqar 501utiorntim Deep Purpie Ketoses liKe
of A-0apthol Soldtion C11
inglobo_ColoUr fructose SucraseS
4 5m Conc: Hcl boi- Qhsent
 PAGE NO.: 16

DATE

Confiumatary jest of quose

0 2 0Sazone test greenisb Yelouo GIucose
0eedle shapad conPitmed
crystale

Bron Glucose
b) Test Soutiont 9odium
resinous Confirmed
hudoxide

C) 3m water dop of methylene Solution is Glacose

blue t im of Sodium hydroxidel.decalaurised Confirmed
Solutiont 2m of gluose SoldtlaD
bai

d) amdaf pata 9sium ferticyanide yellom Colou

salutiont imiol sodium hsdrozid of feri of QLucose
boil.t glucO9e SaldiHon droP bsdroP feuicyanide iscon£itmed
Keep the fluid boiling discharqed-

e)3m Of_ qlucose Solution t tnRed olour GuCOse

of pictic acid Solutian 4imiaP_ dereLopes Confirmed
Sadium hsdiazlde hat

Reg1lt he given Sample LwaS Paund to be

giumse
 PAGENO.:
DATE

Reference
5 R kaje R:R Kale, A ptactical book oP
BLochemigiry and clinical pathologPage
No u6Nitalj prakashan
 PAGE NO.: 18
Experiment No: 0t DATE

Aim To perfoIm qualiiative anaLysis of urine for

tS abnormaConSiiHueDA_

Requitements
a) apearalus: Test tuhesiest luhe holder testtlbe
stand beaker lunnelL ilteE paper ebtc

b) chemicais SuiphoSalycuic acid,utine HNoa chlotoPheok

red Na1C030ceic acid, benedictS reagent
fehlinq reagent AandB Ammania, Saphu
Pawder benzidine Pouwdet laciaL AceHic acid
Hh02 etc
Test observationaference

Test or roteins
i )suiphosalicylic acid test Nhite precipiAlbumin
3m clear urine + Su1phosalicylictate appearprcsent
acid drop by drop

1) Heilers nittic acicd tinq test: ANhile tinqat Albumin

m ofconc. HnIOat add fro.m Side the juncHlonConiimed
Oftest tube dro poige urine OF two fluids

I1) leat c0-aqulation test

5 ma orine t2 drops of_chlotOphena utbidity orAIbuin.
Confirmeed
Ted , adjust the PH Faint pink coloutPrecieftatesS
Duy addinq acete _acid Or 21=
 PAGE NO.: 9

DATE

NAz CO boil tot uo minutes

Add fe drops acetic acid Turbidik Albumin_
remainS ConPirmed

2) Test for SuQaE

1 Benedicts test_
Gree0P Pt qluco.se_present
6m orine t 5mi benedicts
reagent bail for tLoo mLQutes
and Cool uellow pPA glucose
present 21
L) Red ePt qlucose presenE
more than 21.

) fehlings test
2m Pehlinqs At 20 fehlings ed/uellow glucoSee
boi for few mautes, add 2-5 Mppt aPpears Confitmed
urine bail aqainn.

3) Jest for Kekones.

CRothera's test)
5m utine SalidCNHu)2SO4 topetmanqanaja keBones like-
Saturate It comjekelsA 2 drop 0fcoloir gceBone
Sadium DItOpruside Salutian +2melevelops present
Sttonq ammonia 50lution from
Sides Of test tUbe tuai fotL0
minujeS
 PAGE NO.: 20
DATE

4 Test lor bile Saits

6 mA Utine in beaker Sprinkle|PouderSiOks bie Salts
sublimed Sujpher Pouder ofN to bottom present
Sutface

5 Test for bije piqmentS

a Gmelins test
O m 0rine t2-3 drops of dil-Hclcolomtion on Gile pigments
Cilter it Hhtough paper,allo) it aper in falloau preaent
to dryput a drop of Conc. HNO3ng arder g.reen.
at theapex Paper blue violetred
eLnd yelloLoish_
red seeo

bNitic acid test:

am conc aitic acid add Sineplay of bile pigments
otine Sloumly tom Side of test|_co1ours present
iube

6) Test for blood

CBegzidine lesE)

Pinch o benzidinePauder 4 qteen blue Blood

imi of qiacia acid shahe faColodt cue fo Present
minute4 2m utLne t fewiton- be0zidine
crops of H202 faumalo0
 PAGE NO.: 21

DATE

Result
The Orine contoins of albumiOqlucoSe
Kekones.

Reference
SRkaleR.R.kaie: A practical booKof
biache misiq- and clinicaj pathologyPage-
NO. 2.4-25 Mifal_pra Kashan-