Date

Expt. No. Pagc No.

EXPERIME NT NO :1

Aim: Detection o Non- Nittoqeneous unctional qroup in

al6o tunctiOnca
Orpnlc ompounds n Nitroqenous
ghoup

EXPERIMENTS OBSERVATIONS INFERENCES

est for -COOH roup:

KDISSolve _or SuSpend the sample | Ette Vescence CooH aroup
in water and add solhd or With evolution m a y b e present

CO2
22ueoussolution of NaHCO,_Smallf
quantits at a time

2 Test fo Phenalic OH aroup

¥Dissolve the substance in_waterli Reddish ) Simple
addsolid or a few_drops ot colauration a caboxylic acid
Fels solution pp
(Some phenols cive reaction nly
la alchoho! _solulion t isthadreli)Gueen, blue iPhenalic -OH
0dvisable do vepeat the reaction or Violet q10up_present_
in both the media Exceptions are|colouratidon
h-napthal and P- naphthol: The
qive transient qreen colaur thaqnql
Ho white ppt wrth feck3)

Teacher'sSignatue
Reaction for -COOH
ww Youp
R/AT coOH + NaHCO, (0q ethanolic soln) R/Ar-CoOH Co,

Ex
OH
+NaHCO
CoOH COON a
+COt H2
Sali cylic
cid

Reaction for phenolic hyrox-OH) qrouP

Fed3+ Ar- OH >Fe(Aro]GHa
coloured complex an+on
Violet Green/ Red deperds on Ar ring

Reaction for Carbony Group

NO2 - H,o
NO>
o + HN-NH NO =N-NH- NO
(H) R
(H) 2,4 Dinito pheny
aldo/ keto 2,4 Diiopheny
hydrazine hy drazone

P alky/ ary Yellow/ orange / Red

Ralky'/argl/H(alde hyde) Pt ndted
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Expt. No. PageNo. ****

EXPERTMENTS OBSERVATTONS TNFE RENCE

Test for Carbon4l Groupi
* To a small portion of alchoholtcGgan Orange Carbonylqroup
J

solution of he Substance or to or Red ppt present

Small amount ot sample add c=O or -CHo
mnlmum quantits ot qlactal
acetic acid, wam till clear Solu-
son results,then add 2ml_c
Saturated solution_o 2,4- din+tho-
-phenyl hydrazinein alchohol Shake
qetiu heat tobailinq far fe
Minutes Allow to coolL, it no
ppt Appear add 2 drops ot conc
HS04 warm for teW minudes

Caalnand scratch inside of the_

test hube WTth_a alass Yod
Loal and allow to stand

(a) Test for Aldehyde Group (Fehlings Test)

* o asmal portion_ot he
Sub stance add 5 ml Fehlng's Red ppt of -CHO qroup
Soln and then wam on Mater Cuprou6_OxIde preseht

bath o r few minutes

of Fehlings Solution:
Prepavoton
To 2m2 of Fehfing's nand Fehlings
ttle at a ime tillL
Bsoln a

Teacher'sSignature
 3aReaction tor Al deyde GroupP:
Fchling Solut+on Test
A
R-CH=O + 2 CuO C u o + R-CO0OH
Red
R- alkyl ary Cuprous Oxide

This Test not Responded by Ketone:

NaOH
Fehli's Solut+on No 1 : Cuso-aqy 1:1
mxture develop
Na-k-tortovic soln colour ot
Fehlrng's Solution No 2 : deep blue

followin
HOe
0

H O

3bTollens Reaqant Test

A
+ R-coOH
R-CHO+ Ag (NH),1OH Ag
R-CH O +Ago >R-ë-OH 2q
This Test not Responded by Ketone
Mix .cc eadh of AgNO3 soln an d Naott
Tollon's Reaqant
With
sDln Now add NH4OH diopwisse
till clean soln is just obtamed
shoking a
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Expt. No.. Page No.

EXPERIMENTS 08SERVATIONS| TNFERENCES

the bluish white ppt DIssolves

and a clear deep blue soln:
resuHs

Talles Test
*To 5ml of Tollens reagat _add Silver mlrroy -CHO aroup J

a small_amount ok substance and appears om the Present

place the yest tube in a hat walersIdes of test
bath tube
Preparation of Tollen's Reaqant
Tollen's veaqant 1s prepared by ldind Silver mimor
dil NaoH to 5ml AqNo3 soln. Shake appears

Land stand fox eW minut

decant the clar san_and drssolue
the ppt 0 MimuAm volume o t
conc NH3 Soln

4Testn Pamay Amine

Drazo Recaction:
X Add a pinch ot NaNO, toa wellImediate - NH2 q1oup
cooled H (ot -5t) solutiono bilant pYesent
the sample Then pour a little s.carlet of red
this dfazotized solution intu PP
excess ot cold alkaline solutlan ot
B- naptha

Teacher's Signature:
4Reactton for Avomatic 1 amihe:

NaNO,H Ar-NEN Ar-N=N -Q

Ar NH,
o-5t Ar
N
OH M-10 +N=N-Ar

Brlliant zo Due

6) Reaction for Amde Group (-coNH,)}

R-CONH, + NaOH ROONa +Nt3

CH--NH NoH CH-ë-oNa NH

Keotion tor Nitro GvoUp

EtOH/ Ho
Ar NO, +
Zn-NH4 A
Ar -NHOH

Neurtwal
medium Ar-NO+Aq
Black or Gvay
PPt
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Expt. No.. Page No..

E XPERIMENTS OBSERVAT IONS INFERENCES

Test far Amide naup:
*_Heat o.5qm of the sample ith Smell of NH -CONNH2 Dup
2 ml of 50 NaoH Solrtion. which turns preset
Hq (NOaper
black
Test far Nitan bnaup Mallken_and, BasersTest)
Dissolve_a litle ot the _sample
in 5m 50 alcohal -then add a LA black _ay qiay|No qmip
J

htle solhd Nti4Ck in 10% Cad ppt PHescnt

soh cand a pinch ot Zn dust Bail
Hor tewAutes and uen-ilter
the above reaction product into Sml
of Tollen's Yeaqant (Aerno AgNo,saln
and wArm on water bath

leachersSgnature