Raje Laxmansingh Bhonsle College

of Pharmacy, Akola
D.Pharm – II Year
Experiment No. 28

Title: To Prepare and submit computerized report of Expt. No. D8 (Any one
compound from D1 to D10) By Systematic Qualitative Analysis.
A) PELIMINARY TEST

Sr
Test Observation Inference
No.
A) Colour Yellow-Orange
Nitro Anilines
B) Odour Phenolic
Phenol

Solubility Behaviour
C) 1) Solubility in Water Insoluble Insoluble
2) Test with Litmus Acid Acids or Phenol

Action On Reagents Action of Cold NaOH:

About 0.2 gm/3 drops if compound +2ml of Acid or Phenol may
F) Completely Soluble
be present
water + 2 ml of 10% NaOH and the mixture is
shaken well.

Conclusion: On basis of the test performed above and with reference to the handbook the
given organic compound / drug is:

1)Aromatic

2)Unsaturated

3)Acid
 B) DETERMINATION OF PHYSICAL CONSTANT

Conclusion: The boiling of the given organic drug or compound was found to be 182℃

C) DETERMINATION OF ELEMENT
Sr
Test Observation Inference
No.
Test for Nitrogen: 3-4 ml filtrate + Solid
A) No blue green colour "N" absent
FeSO4 Heat, to boil with Conc. H2SO4

Test For Sulphur: 2ml filtrate + drop of dil.

No black ppt is
B) NaOH soln + 4 drops of freshly prepare dil. "S" absent
produced
Soln of Sodium Nitroprusside

Test for Halogen: 5ml filtrate + dil. H2SO4 Boil no white colour is
C) "Cl" absent
+ add few drops of dil. HNO3 add AgNO3 soln produced

D) DETERMINATION OF FUNCTIONAL GROUP {FOR N, S, C, H, O}

Sr
Test Observation Inference
No.
Dissolve and
Test for Phenol: 0.5ml if Sodium Hydroxide Acid Phenol may
A) repeated than conc.
+substances be present
HCL

Conclusion: The given organic drug or compound was found to contain phenol [−𝑂𝐻]
functional group.
Result: The given organic compound was having 182℃ bp, O, H elements and [−𝑂𝐻]
functional group.
Therefore, with reference to literature, the organic compound may be Phenol.

E) CONFERMATORY TEST
Sr
Test Observation Inference
No.

Ferric Chloride test: to aq. Soln of Phenol or Violet, Blue, Green

A) Phenol present
crystal + few drops of Ferric Chloride soln colours are obtained

F) STRUCTURAL FORMULA AND CATEGORY

Structure:

[Phenol]
OH

Category: Antiseptic
Therefore, the given compound with code D8 is confirmed.

Phenol

SIGNATURE OF ANALYST
(RIDA FATEMA)

NAME: RIDA FATEMA AFROZ KHAN INAMDAR

ROLL NO. 34

SIGNATURE OF FACULTY: ____________

DATE: __/__/____