HL-3125Plus

Hematology analyzer
Service Manual
Contents
1. INSTRUMENT JOINT.......................................................................................... 4
2. REAR PANEL ....................................................................................................... 5
2.1 Top right is the I/O part. ............................................................................5
2.2 Reagent connection....................................................................................6
2.3 Diluent connection .....................................................................................7
2.4 Detergent Connection ................................................................................8
2.5. Lyse Connection........................................................................................9
2.6. Waste connecting.....................................................................................10
3. THE INSTALLATION OF PRINTER AND PAPER ...........................................11
3.1 Thermal Printer........................................................................................ 11
3.2 External printer installation and setting..................................................12
4. HYDRAULIC PART ........................................................................................... 19
4.1 WBC counting chamber module ..............................................................19
4.2 RBC counting chamber module ...............................................................20
4.3 Right panel assembly module...................................................................21
4.4 The real physical picture..........................................................................22
4.5 Reagent detection tube module ................................................................23
4.6 Plastic Turntable Module.........................................................................24
4.7 Syring module ..........................................................................................26
4.8 The real hydraulic assembly figure ..........................................................28
4.9 Solenoid Valve ..........................................................................................29
5. THE ELECTRIC PART STRUCTURE............................................................... 32
5.1 Mother Board...........................................................................................32
5.2 Driving board...........................................................................................33
5.3The real assembly Figure..........................................................................34
5.4 Printer control board ...............................................................................35
5.5 Electrical Connection ..............................................................................36
6. FLOW CHART .................................................................................................... 37
 6.1 HL-3125plus flow chart ...........................................................................37
7. THE MAINTENANCE OF INSTRUMENT....................................................... 37
7. 1 Daily Maintenance..................................................................................37
7.2 WEEKly Maintenance ..............................................................................38
7.3 Monthly Maintenance ..............................................................................38
8. TROUBLE SHOOTING...................................................................................... 38
8.1 Aperture blocking.....................................................................................38
8.2 Poor repeatability ....................................................................................41
8.3 Abnormal test results................................................................................41
8.4 Adjust for differential ...............................................................................42
8.5 HL-3125plus HGB Volt Check & Adjust..................................................43
8.6 Valve Check ..............................................................................................45
8.7 Piston Lubrication ...................................................................................47
9. UNIT ADJUST .................................................................................................... 49
10. DATA TRANSMIT ............................................................................................ 50
1. INSTRUMENT JOINT
The function of front panel

logo

display

light

Model nameplate

Aspiration switch
Power switch
Internal
thermal
printer

Figure 1-1
The introduction for the front panel：
1.1 company logo
1.2 display
1.3 Indication light
1.3.1 There are 3pcs light in HL-3125plus. The functions are as follows.

Power light: Green, always on after switch on.

Working light: shows green during stand by. Shows yellow during working.
Error light: For example, if there is no reagent, it would turn on. It is off in
normal working and shows red when there is error.

1.4 Instrument model

1.5 Aspiration switch: For starting test, its function equals to the “Start” button in the software
interface.
1.6 Internal Thermal printer: For printing the test results.
1.7 Power button: For switch on and off the instrument. If the instrument is forced to switch off,
press and hold the button for 3 seconds, the instrument would shut down automatically.

-4-
2. REAR PANEL

2.1 Top right is the I/O part.

VGA port
RS232-1

USB-2
Parallel port

LAN Internet Port

RS232-3 USB-1

RS232-2
DC 12V input

Equipotential
connection
Figure 2-1
I/O board functions:
2.1.1 RS232-1：for transmit data
2.1.2 Parallel port：for connecting external specified printer.
2.1.3 USB-1：for connecting USB device. When using USB printer, please separately connect
with this interface.
2.1.4 RS232-2：for transmit data. The funtion is now not available.
2.1.5 RS232-3：for transmit data. The funtion is now not available.
2.1.6 USB-2：for connecting USB device(such as mouse, keyboard).
2.1.7 VGA port：When burning the specified software, it could be used for output of video
signal to display or projector as training and demonstration.
2.1.8 Equipotential connection: for ground.
2.1.9 DC12V input: power input terminal and for connecting power adapter or battery.

-5-
2.2 Reagent connection

Lyse connector(blue)

Detergent connector (green)

Diluent connector(white)

Waste connector(red)

Figure 2-2
Reagent connector
2.2.1Waste Connector (red): for connecting waste tube.
2.2.2 Diluent connector(white): for connecting diluent tube.
2.2.3 Lyse connector (blue): for connecting lyse tube.
2.2.4 Detergent connector (green): for connecting detergent tube.

-6-
 Reagent connection

2.3 Diluent connection

2.3.1 Take out the diluent tube with white cap from accessories bag.

White reagent tube cap

Figure 2-3
2.3.2 Take out the diluent and remove the cover.

Figure 2-4
Note：The reagent shall not be contaminated during the process.
2.3.3 Put another end of the tube into the diluent and turn on the diluent cap clockwise.
2.3.4 Connect the white reagent tube cap to the diluent connector (white) in the back of the
instrument and turn it tightly clockwise.

-7-
Note：
a. The joint circled in the figure 2-3 shall be attentioned carefully. It can not be pinched and
would be easy to loose, take off, which would cause air bubble inside the tube.
b. The reagent tube cap shall be turned off tightly. The joint is easy to loose and leak. It shall
be routinely examed.

2.4 Detergent Connection

2.4.1 Take out the detergent tube with green tube cap from accessories bag.

Green reagent tube cap

Figure 2-5
2.4.2 Take out detergent bottle. There shall be sufficient detergent in the bottle. Remove cap.

Figure 2-6
Note：The reagent shall not be contaminated during the process.
2.4.3 Put another end of the tube into the detergent and turn on the cap clockwise.
2.4.4 Connect the green reagent tube cap to the detergent connector (green) in the back of
the instrument and turn it tightly clockwise.

-8-
Note: The joint circled in the figure 2-5 shall be attentioned carefully. It can not be pinched
and would be easy to loose, take off, which would cause air bubble inside the tube.

2.5. Lyse Connection

2.5.1 Take out the lyse tube with blue tube cap from accessories bag.

Bule Reagent tube cap

Figure 2-7
2.5.2 Take out lyse bottle. There shall be sufficient lyse in the bottle. Remove cap.

Figure 2-8
Note：The reagent shall not be contaminated during the process.
2.5.3 Put another end of the tube into the detergent and turn on the cap clockwise.
2.5.4 Connect the green reagent tube cap to the lyse connector (blue) in the back of the
instrument and turn it tightly clockwise.
Note: The joint circled in the figure 2-7 shall be attentioned carefully. It can not be pinched
and would be easy to loose, take off, which would cause air bubble inside the tube. If the lyse
could not be aspirated sufficiently, the results for WBC and HGB would be abnormal.

-9-
2.6. Waste connecting

2.6.1 Take out the waste tube with blue red cap from accessories bag.

Waste Barrel cap

Red reagent tube cap

Figure 2-9
2.6.2 Connect the red reagent tube cap to the waste connector (red) in the back of the
instrument and turn it tightly clockwise.
2.6.3 Put another end of the tube into the waste barrel and turn on the cap.

Note:
1. The tuebe of waste shall be the same size with dilute tube. And thicker than detergent
and lyse tube. Please pay attention to it when make installation or replacement, otherwise
there would be bubble error alarm.
2. When the user move the instrument or the nearby articles, sometimes would
mistakenly press the waste tube which lead the abnormal of discharge of the waste. It would
cause the abnormal noise, bubble error alarm and leakage, etc.
3. Always check if the red cap become aged and block the tube.

The precautions when connecting reagents

1. After connect all the reagent tubes, ensure the tubes are all in natural status, no
pinch, no distortion.
2. All the reagent tube and cap connecting shall be done by hand. Any tool is
forbidden.
3. The reagents used shall be matched with instrument. Otherwise it could make the
incorrect test results and differential. The hydraulic part of the instrument would be
damaged if any serious situation happened.
4. The connection of the reagents shall avoid direct sun light beat.
5. When connect or replace the reagent, please avoid the reagent tube contacting with
other articles in case reaegnt contaminated.
6. Please do not use the iced reagents.
7. Please do not use the expired reagents. Please use the reagents before its expiry
time indicated in the packing.

- 10 -
 8. The reagents shall be put in the same level with the instrument. The waste barrel
shall be put a little lower than the level of the instrument.

3. THE INSTALLATION OF PRINTER AND PAPER

3.1 Thermal Printer

3.1.1. Use the finger press the position pointed by the red arrow. Press it and open the printer
door.

La

Figure 3-1
3.1.2 The introduction of the thermal printer

Indicator light
SEL key

LF key

sensor
Pressing roller

Thermal paper

Figure 3-2
A. “LF” key: for setting the status of printer (detected by the status indicator light). When the
light is on, it is connecting status and waiting to receive the print command. When the light
is off, it is disconnecting status, it can not receive command.
B. “SEL” key: After fix the paper, press the key. The printer head would automatically feed
paper. Press again the key. It would stop and enter connecting status.
C. Indicator light: There three kinds status: The first is always on in connection status; The
second is off in disconnection or power off status; The third is flickering in out of paper. When
the printer has problem not printing, some problems could be detected by the indicator light
status.

- 11 -
D. Cut the new thermal paper as beveled edge and aim at the lower part of compression bar
of printer head and insert inot it. The printing side face downwards.
The detection method for printing side: Use nail to scratch the paper or close to heat. The
side changing color is printing side. If both sides would not change color, it is not thermal
paper. It then can not be used in the instrument. Pleases see figure 3-3。

Figure 3-3
E. After sense the signal, the printer would automatically feed paper. When the paper comes
out from aother side, press the “LF” key, the printer indicator light becomes off. Press again
the “SEL” key to feed some more paper. And press the “LF” key to stop the feeding. Please
put the paper into the printer box and close the printer door as figure 3-4。

Figure 3-4
Note:
1. The width of thermal printer papaer is 57mm
2. The thermal side of the paper shall face downwards.

3.2 External printer installation and setting

3.2.1 The step for external printer installation

(1) It is unnecessary to install separately the driver for external ink-jet printer and mini
stylus printer.
(2)Enter the interface “service” from the main menu. Choose “Check” and input the
service password “72117211” to the service check interface.

- 12 -
 Click “Adapter” as shown in 3-5. Click the bottom right “Function” button to the interface
showed as figure 3-6.

- 13 -
Figure 3-5 Service Check interface

Figure 3-6 Adapter Setting

- 14 -
 Figure 3-7 Function Interface
(3) Please select HP P2035 if connecting with laser printer. Click “Step 1”, shown as figure
3-7, click “Step 2” after restart the instrument.

Figure 3-8 Steps for printer driver installation

(4) Select “EPSON” if connecting with stylus printer. Choose the printer model as shown in
figure 2-3. Click “Step 1”, shown as figure 3-8 and click “Step 2” after restart the instrument.
3. 2.2 The steps for printer setting
(1) External laser printer
①HP P2035；Model: HP P2035

- 15 -
②Prerequisite: The instrument already has been installed the printer driver. Before switch on
the instrument, connect the cable to the instrument through USB port and connect the power
cable.
③First, in the main menu, enter “setting”, find print settings. Make the setting of the
printer as shown in Figure 3-9. Select “laser printer + Net” or “laser printer”, click “Save”.

Figure 3-9 Printer setting interface

④Enter the interface “service” from the main menu. Choose “Check” and input the service
password “72117211” to the service check interface as figure3-5. Click Function of “Printer

reset” to the interface as shown in figure 3-10.

Figure 3-10

- 16 -
⑤ Select “file” —“Print” in the top left of the interface “Print Restore”, then the figure 3-11
shows.

Figure 3-11 Printer setting interface

Choose “PCL Inkjet” in the option “Printer (P)”; Choose “LPT2” in the option “Port (O)”. Then
click the “OK” on the top right to make font restore print.
(2) External Stylus printer
① Model: EPSON LQ-590K/EPSON LX-300+II；

② Prerequisite: The instrument already has been installed the printer driver. Before switch
on the instrument, connect the cable to the instrument through USB port and connect the
power cable.
③ Make the setting of the printer as shown in Figure 2-4. If the model is EPSON LQ-590K,
select “Stylus PRT2” or “Stylus PRT2+Net” or “Stylus PRT2(A4)” or “Stylus PRT2(A4)+Net”; if
connect with EPSON LX-300+II, select “Stylus PRT3” or “Stylus PRT3+Net” or “Stylus
PRT3(A4)” or “Stylus PRT3(A4)+Net”. Click ‘Save’.
④ Enter the interface “service” from the main menu. Choose “Check” and input the service
password to the service check interface as figure 2-1. Click Function of “Restore fonts print”
to the interface as shown in figure 2-5. Select “file” —“Print” in the top left of the interface
“Print Restore”, then the figure 2-6 shows.
Choose “EmboPrinter” in the option “Printer (P)”; Choose “LPT2” in the option “Port (O)”.

Then click the “OK” on the top right to make font restore print.
Note:

- 17 -
(1) When set the printer as “Stylus PRT2” or “Stylus PRT2+Net” or “Stylus PRT3” or “Stylus
PRT3+Net”, it is applicable to the multipart forms print mode. The paper feed of relevant
printer shall be set as multipart forms. The LQ590K is set as “FF-FUSH” below “CUT” and the
left side set as “1”. For the LX-300+II, it is only furnished with one option for multipart forms.
That is the lower shift. The left side is set as “1”.
(2)When set the printer as “Stylus PRT2 (A4)” or “Stylus PRT2 (A4) +Net” or “Stylus PRT3
(A4)” or “Stylus PRT3 (A4) +Net”, it is applicable to the A4 paper. The paper feed of relevant
printer shall be set as single page. The LQ590K is set as “CUT” and the left side set as “0”.

For the LX-300+II, it is single page mode. That is the upper shift. The left side is set as “0”.
Note:
(1) During the process of print restore, if there is no required option occurring in printer and
port selections, please confirm if the cable is connected correctly and the printer is switch on.
If the equipments are confirmed correctly connected, there is still no option. Please restart the
instrument and try again.
(2) Because the external printers all connected through USB port, in order to avoid the
interaction of the different drivers, it would be best that the service engineer install the printer
driver as per the customer’s demand when installing the instrument.

- 18 -
 4. HYDRAULIC PART

4.1 WBC counting chamber module

HGB Plug

Outer electrode

Bar code

WBC 插头
Inner electrode

HGB receiver

Buffer cup（lengthen）

Figure 4-1
WBC counting chamber introduction:
4.1.1 Outer electrode：Lug used for connecting with right panel assembly module. Another
end connects with outer shield wire of WBC plug, which take the effect as ground.
4.1.2 Bar code: for distinguish the production date and batch of the module. Take effect of
traceability.
4.1.3 Inner electrode: Inside the counting chamber and connect with reagent. The positive
pole connected with the point here when counting and together with outer electrode to act on
the both ends of aperture.
4.1.4 HGB receiver: receiving light signal and convert it into electric signal.
4.1.5 Buffer cup: when discharge counting chamber, the waste would be discharged from it.
When make mixing, the positive pressure acts on the reagents in counting chamber
upwards and take the buffer effect.

- 19 -
 4.1.6 HGB Plus: The 4pin plug, of which red and black wires are for power of HGB launch
tube. The shielded coaxial wire is output of HGB receiver and connects to the drive plate
HGB outlet.
4.1.7 WBC Plug: Composed by a coaxial cable and plug for the output and feedback of
aperture voltage.

4.2 RBC counting chamber module

Outer electrode
Bar code

RBC Plug

Inner electrode
J2 identification label

Buffer cup（lengthen）

Figure 4-2
RBC counting chamber introduction:
4.2.1 Outer electrode：Lug used for connecting with right panel assembly module. Another
end connects with outer shield wire of RBC plug, which take the effect as ground.
4.2.2 Bar code: for distinguish the production date and batch of the module. Take effect of
traceability.
4.2.3 Inner electrode: Inside the counting chamber and connect with reagent. The positive
pole connected with the point here when counting and together with outer electrode to act on
the both ends of aperture.
4.2.4 Buffer cup: when discharge counting chamber, the waste would be discharged from it.
When make mixing, the positive pressure acts on the reagents in counting chamber upwards
and take the buffer effect.
4.2.5 RBC Plug: Composed by a coaxial cable and plug for the output and feedback of
aperture voltage.
4.2.6 J2 identification label: The double-chamber instrument installed with two counting
chamber module. It is used for distinguish WBC and RBC counting chamber to avoid wrong
insert.

- 20 -
4.3 Right panel assembly module

Bar code

Stainless ground tube A

O Ring

aperture

Figure 4-3
Right panel assembly module introduction:
4.3.1 O Ring: Acts on the counting chamber module and right panel assembly and take the
effect of sealing.
4.3.2 Bar code: for distinguish the production date and batch of the module. Take effect of
traceability.
The PN for right panel assembly module: MHL-800-026 is the PN for 80μm. The
MHL-800-026-5 is the PN for 50μm.
4.3.3 Aperture: The cell passing through aperture would change the voltage and cause the
pulse. Collecting and processing the pulse could finalize the counting for the cell volume and
quantity.
4.3.4 Stainless ground tube A: Located behind the aperture, and fixed with glue. Screw
mounting hole for connecting external electrodes of measuring cup module, making loop for
apertrue voltage, takine effect as ground.

- 21 -
4.4 The real physical picture

HL-3125plus real instrument photo

Figure 4-4

- 22 -
4.5 Reagent detection tube module

Label

2pin Plug

Stainless detection tube B

Sampling tube

Figure 4-5
Introduction of reagent detection tube module:
4.5.1 2pin Plug: connecting driving board, for transmitting the resistance tested in two pieces
detection tube B.
4.5.2 Label: There are three kinds of reagent diction tube module. The 16, 17, 18 are
separately matched with the J16, J17 and J18 in the driving board. Also correspond to lyse,
detergent and diluent. The label is sued for distinguish the corresponding relation.
4.5.3 Sampling tube: testing the resistance of the reagents in the testing tube.
4.5.4 Stainless detection tube B: Located behind the aperture, and fixed with glue. Screw
mounting hole for connecting external electrodes of measuring cup module, making loop for
apertrue voltage, takine effect as ground.
If there is bubble in the tube or the bad welding of the stainless tube, these issues would
cause the resistance growing big and instrument would give reagent false alarm.

- 23 -
 4.6 Plastic Turntable Module

J22 Optocoupler

Belt guide
Pressing pully & bracket

Probe fix ring

Note: This screw is easy to losse

Belt fixer and can not be tightened. Please
do not repeat adjusting.

Probe

Timing belt

Bar code
Aspirating rotate cover

Probe cover

Plastic turntable

Figure 4-6

Figure 4-7 Probe cover

- 24 -
Plastic turnable module:
4.6.1 J22 Optocoupler: Control the probe position through the block plate above the belt fixer.
4.6.2 Belt guide: for fixing the travel direction of belt fixer.
4.6.3 Probe fix ring: Fixing the probe on the belt fixer.
4.6.4 Belt fixer: Fixing belt and fix the probe through probe fix ring. And it could drive the
probe up and down.
4.6.5 Probe: for aspirating sample and dispense the diluent.
4.6.6 Probe cover: for cleaning the residual samples and dispense diluent.
4.6.7 Aspirating rotate cover: installed on the probe cover(clockwise for tight, counter
clockwise for loose) for pressing the internal “O” ring to avoid the leakage of diluent.
4.6.8 Presssing pully & bracket: After installing belt, adjust the position of pressing pully
bracket to avoid the belt creep.
4.6.9 Timing belt: make probe moving up and down through the support of up & down motor
and MXL timing wheel driving belt fixer.
4.6.10 Bar code: for distinguish the production date and batch of the module. Take effect of
traceability.
4.6.11 Plastic turntable: the carrier for above mentioned materials. There is driving gear in the
back side and is controlled by left & right motor, which could make the complete module
moving left and right.

- 25 -
4.7 Syring module

4.7.1 H08 Syringe module for HL-3125Plus

Waste suck and positive

pressure discharge connector
Waste discharge connector

Piston B (lyse)
Piston B(diluent)

Bar code

Waste piston

液压支架导轨
H08 hydraulic guide rail

Figure 4-8

Sample aspiration and

probe dispensing diluent

J24 opto coulper

Waste
connector

Lyse connector Diluent connector

Figure 4-9

- 26 -
 The hole is used
for use silicon
oil, insert M2
screw driver
into it to roate
the pistion to
another side to
lubricate.
Note: Only the special
lubricant provided by
manufacturer could be used
and prohibit to use
lubricants of other materials

Figure 4-10
Note:
1. The position of optocoupler of H08 syringe moudle is no need to adjust. The module would
not shake when reset, unless the optocoupler or other circuit failure.

- 27 -
4.8 The real hydraulic assembly figure

4.8.1 HL-3125plus

Figure 4-11

- 28 -
4.9 Solenoid Valve

4.9.1 TAKASAGO solenoid valve (Japan)

4.9.1.1 Two-way valve

Voltage & Pressure

Input Output

Figure 4-12
Introduction for two-way valve
4.9.1.1.1 Voltage and pressure: for distinguish the voltage and maximum pressure range.
4.9.1.1.2 Input(IN): The input port of reagent or pressure.
4.9.1.1.3 Output(OUT): The output port of reagent or pressure.
Note: When replacing valves, the installation direction shall be strictly operated as the
demands.The input and output could not be installed reversely. Otherwise, the instrument
performance would be affected during long time testing. Please record the default installation
before make replacement.
4.9.1.2 Three-way valve

Voltage & Pressure

NO

COM

NC

Figure 4-13

- 29 -
Introduction for two-way valve
4.9.1.2.1 Voltage and pressure: for distinguish the voltage and maximum pressure range.
4.9.1.2.2 COM: The por for reagent and pressure pass through.
4.9.1.2.3 NO: Connect with COM when the valve is powered off. When the valve is powered
on,it would be disconnected with COM.
4.9.1.2.4 NC: When the valve is powered off, disconnect with COM. When the valve is
powered on, it would be connect with COM.
4.9.1.3 Internal structure

loop

Spring

Core cover

Moveable diaphragm

Diaphragm

Valve head

Figure 4-14
Instructions for internal structure (take sample of the two-way valve of figure 4-48).
4.9.1.3.1 Loop: produce magnetic field when power on.
4.9.1.3.2 Spring: When power off, push the core downwards to rest the position.
4.9.1.3.3 Core: transmitting magnetic field produced by loop.
4.9.1.3.4 Moveable diaphragm: fixed between valve head and cover to limit the moving
distance, which can avoid the shake of core.
4.9.1.3.5 Diaphragm: keep apart the two tube mouth on valve head when the loop is powered
off.
4.9.1.3.6 Valve head: The passing way of reagent or pressure. Also the wholes for fixing
screw.
4.9.1.3.7 Cover: protect loop and label.

Note:
1. The place between diaphragm and valve head would appear reagent crystal or any other
articles blocking. It would cause the incomplete closing of the valve.
2. If open for cleaning, please remember to put complete moveable diaphragm into the valve
head before assembling other parts.

- 30 -
3. Please pay attention to the position of the valve head incase any reversely installation.

4.9.2 Burkert valve (Germany)

Plug

Valve boday
Valve body

Port mark

Valve head

Port
Port “IN” Valve left（NC）, middle(COM),
“OUT”
head rightNO）

Figure 4-15

Label

Figure 4-16
The fuctions of each part are the same as TAKASAGO valve.

- 31 -
 5. THE ELECTRIC PART STRUCTURE

5.1 Mother Board

USB DEVICE Joint

Power Joint

SD card
TFT LCD

Label

STN LCD

Cell

Keyboard

JTAG
Printer

UART+USB DM9000+VGA

Figure 5-1
Introduction for mother board
5.1.1 USB DEVICE Joint: used for burning NK and programme. It would not be used in
normal operating situation.
5.1.2 Power Joint: +5V and +12V
5.1.3 SD Card holder: for connecting SD card. There is barcode and label on the bottom.
5.1.4 Labe: The SN of supplier for tracking source.
5.1.5 Cell: for 3V button cell for clock.
5.1.6 JTAG: A kind of international standard testing protocol (IEEE 1149.1 compatibility).
Mainly for chip internal testing.
5.1.7 UART+USB: UART is a kind of universal serial data bus, for asynchronous
communication. The bus is bidirectional way communication. The full-duplex transmission
and reception could be finalized. It is the serial. USB interface is or connecting the USB
cable.
5.1.8 TFT LCD: Output TFT signal for connecting LCD cable.
5.1.9 STN LCD: Output STN signal for connecting STN display. It is Not Applicable for the
moment.
5.1.10 Keyboard: for connecting 5*5 small keyboard. It is Not Applicable for the moment.
5.1.11Printer: Output parallel print signal for connecting internal termal printer or External
parallel printer.

- 32 -
 5.1.12 DM9000+VGA: Net and VGA signal for conneting net or VGA signal.
5.1.13 60pin: At the back of the mother board for connecting driving board to input & output
each controlling signal.

5.2 Driving board

5.2.1 HL-3125Plus driving board

J1~J17

J18
JP5
J31~J35

potentiometer
Burning
programme mark

J20~J25
JP1

J26 J28
JP2
J27
J29

Figure 5-2
Introduction for drivng board
1. Power socket: +5V and ±12V supplied by switch power.
2. 60 pin socket: connect with 60pin on mother board, input and output each controlling
signal.
3. Potentiometer: for adjusting HGB value. There shall be a certian quantity diluent inside the
counting chamber before adjusting HGB value.
4. JP1: WBC signal input, connecting WBC plug.
5. JP2: RBC signal input, connecting RBC plug.
Note: HL-1300 only has one counting chamber, so only connecting WBC plug.
6. JP3: HGB signal input, connecting HGB plug.
7. J1~J11: correspondingly connect 1#~11# valve
8. J12: buzzer interface
9. J13: Aspiration switch interface (HL-3000 series driving board). The other instrument like
HL-2400 and 1300, the interface here is idle.
10. J14: Door swtich interface.

- 33 -
11. J15: Aspiration switch interface (HL-1300/2400 driving board). The other instrument like
HL-3125Plus, the interface here is idle.
12. J16: Lyse detecting interface. The interface is idle in HL-3000 series driving board.
13. J17: Detergent detecting interface. The interface is for singal of out of paper for printer.
14. J18: Diluent detecting interface. The interface is idle in HL-3000 series driving board.
15. J20, J22, J24: Optocoupler signal. Separately connect with 20#, 22# and 24# coupler.
16. J21, J23, J25: Output for motor controlling. Separately connect with 21#、23#、25# motor.
17. J26: Input port for switch on & off controlling signal. Connect with touch switch.
18. J27: DC 12 V power input port.
19. J28: Idle
20. J29: Power output port, output 5V and 12 V to mother board and IODB5 board.
21. J31~J35: The connection for the 5pcs status light on the front cover.
22. JP5: CPLD chip burning program interface.
23. Burning program mark: The clicked “PASS” means the driving board has been burned
program.

5.3The real assembly Figure

5.3.1 HL-3125plus

Figure 5-3

- 34 -
5.4 Printer control board

Print signal input,

connect I/O board power

Figure 5-4

Print head interface

Figure 5-5

- 35 -
5.5 Electrical Connection

Figure 5-6

- 36 -
6. FLOW CHART

6.1 HL-3125plus flow chart

Figure 6-62

7. THE MAINTENANCE OF INSTRUMENT

The importance of the maintenance

Since the hematology analyzer is composed of hydraulic, pneumatic and electric parts. In
order to get the best working status, the instrument need the routine maintenance. The
frequency of maintenance would affect the accuracy and stability of the instrument.

7. 1 Daily Maintenance

1. The start up shall be operated when first switch on the instrument.

2. The showdown program shall be strictly executed after one day work.
3. After 30 samples, make a “maintain” program.
a) Prepare cleanser and probe cleanser, if you do not have probe cleanser, just using
cleanser is okay .
b) In the main menu window, press the right key of the mouse, select
“Service/Maintain”. Operate as the screen shows.

- 37 -
7.2 WEEKly Maintenance

Drop the cleanser or concentrate cleanser into the RBC and WBC cups. Soak the cups for
half an hour.

7.3 Monthly Maintenance

Use the silicon oil in the accessories bag to lubricate the white piston.
Use the solid grease to lubricate the two bars (pointed by red arrow) in the following picture.
Please note only the solid grease, the liquid grease is forbidden to use.

If the instrument has not used for one week, please make “suspend” program in the “serve”
menu to ensure the cleaning of the hydraulic part. Routinely check the reagent tube
connection, probe cover, probe, plastic turntable module and syringe module. The relevant
adjustment or replacement is required.

8. TROUBLE SHOOTING

8.1 Aperture blocking.

Problem: After blocking, the test results would be lower or showing “***”( Because
HL-3125Plus has the aperture voltage testing function, when the tested aperture voltage
showing abnormal, the matched channel testing results would show “***”）
。
Analysis:
8.1.1 The testing sample volume is big, lack of maintenance. The instrument is easy to block.

- 38 -
8.1.2 Reagent expired or out of reagent or unoriginal reagents causing the fake block.
8.1.3 Circuit fault
Solutions:
1. Make the cleaning and enhanced cleaning to remove blockage. Take out aperture making
soak or replacement.
2. Replace with original reagent.
3. Check the log, if long time not making “start up”, the instrument would give fake blocking.
Note: Each time executing “Start Up” with the panel close (making “Start Up”, the two panels
of the instrument shall be closed), the instrument would collect the aperture voltage in two
times blanking testing. If long time not makes “Start Up” with panel close, the fake block
would happen. So the “Start Up” with panel close shall be made after replacing aperture.
4. Check if the aperture voltage is between 0.7~0.8. Replace the driving board if confirm the
problem.
8.1.4 When the test results coming with star *** or “Hole plugging holes suspected, please
re-test”, it maybe caused by the defect of aperture, chamber or driving board.
But it also maybe caused by the improper recording of the aperture voltage by the instrument.
The followings are the procedures to enter the interface of checking aperture voltage:
1. In the“Main Menu”, click Service”to“Serve”interface.

Figure 8-1 “Main Menu”

2. Click “Check” in serve interface. The password request window will pop-up. Make the
operation as the figure 8-2 and 8-3 as follows. Click “Key On ”, the soft keyboard will appear,

- 39 -
input “7211”.

Figure 8-2 “Serve”interface Figure 8-3, input the password

3. In the “Check” interface, click “Sample” to enter sample interface. Make the operation as
shown in figure 8-3 and 8-4.

Figure 8-3 “Check”interface Figure 8-4 “Sample”interface

4. As shown in figure 8-4, in the red circle, the aperture voltage V1 and V2 are shown. “V1” is
for WBC aperture voltage. “V2” is for RBC aperture voltage。Click “T” for checking the
current aperture voltage. The normal range of the voltage shall be around 0.7-0.8 more or
less.
If the voltage is beyond the range, please make clean or flush in the service menu. And then,
make “Shut down” program, after that, switch on and make the “Start Up” again. Please close

- 40 -
the panel during the whole process. In this way, the instrument would record the new voltage
value.
If the problem still could not be solved, then the aperture, chamber, tube connection and
driving board are all need to consider and check.

8.2 Poor repeatability

Problem: When the blank testing is qualified, testing one same sample, the discrepancy of
test results for one item or many items is big.
Analysis:
1. The mixing of the blood sample and operation method.
2. Reagent contaminated, expired or short.
3. The dispense volume of lyse is not sufficient.
4. The pneumatic system has problem, the positive pressure and vacuum are not stable.
5. Other outside interference.
Solutions:
1. Improve operation method to ensure the good mixing. Use venous blood for testing could
reduce the interference of human factors on the stability of the test.
2. Replace the reagents.
3. Check the valve 9 and relevant tube concerning with lyse.
4. Open the left panel of the instrument and check the movement of the instrument part
during mixing and discharge. And make the maintenance of the syringe module, like
lubrication, etc.
5. Check if the installation surrounding meets the requirements. Especially the position of the
reagents, is there any interference? The power supply and ground(≤0.5V) shall all be checked.

8.3 Abnormal test results

8.3.1 WBC, HGB test results are correct, but RBC and PLT are low.
Analysis:
1. In plastic turntable module, the belt is too tight which make the probe can not reach its
position. Then the second aspiration volume is not reach the required 20μL.
2. The dust between belt guide and belt fixer produce resistance which makes the probe can
not move smoothly up and down. It also makes the short aspiration volume.
3. Aspirating rotate cover is fixed too tight affect teh moving of probe, especially in low
temperature.
Soultions:
1. Adjust belt.

- 41 -
2. Remove dust and make lubrication.
3. Pay attention to the aspirating rotat cover, not fix too tight.

8.4 Adjust for differential

1. In the“Main Menu”, click “Service”to“Serve”interface.

Figure 8-1 “Main Menu”

2. Click “Check” in serve interface. The password request window will pop-up. Make the
operation as the figure 8-2 and 8-3 as follows. Click “Key On ”, the soft keyboard will appear,
input “72117211”.

Figure 8-2 “Serve”interface Figure 8-3, input the password

- 42 -
3. In the “Check” interface, click “Adapter” to enter sample interface. Make the operation as
shown in figure 8-5 and 8-6.

Figure 8-5 “Check”interface Figure 8-6 “Sample”interface

4. In the Column of WBC, RBC and PLT, you can change the value to adjust the differential
and histogram.
For the target value, you can take reference of user manual Chapter 3 – 3.7 Graph Adjust to
find the value.

8.5 HL-3125plus HGB Volt Check & Adjust

The followings are the procedures to enter the interface of checking aperture voltage:
1. In the“Main Menu”, click Service to“Serve”interface.

- 43 -
 Figure 8-1 “Main Menu”
2. Click “Check” in serve interface. The password request window will pop-up. Make the
operation as the figure 8-2 and 8-3 as follows. Click “Key On ”, the soft keyboard will appear,
input “72117211”.

Figure 8-2 “Serve”interface Figure 8-3, input the password

3. As shown in figure 8-7, there is HGB item. Click “T” for checking the current voltage. The
normal range shall be 400-1000.

Figure 8-7 “Check”interface

- 44 -
If the value is not in the correct range, please adjust the potentiometer in the driving board.

potentiometer

Please adjust the potentiometer while click “T” beside HGB.

8.6 Valve Check

The followings are the procedures to enter the interface of checking valves:
1. In the“Main Menu”, click “Service”to“Serve”interface.

Figure 8-1 “Main Menu”

- 45 -
2. Click “Check” in serve interface. The password request window will pop-up. Make the
operation as the figure 8-2 and -3 as follows. Click “Key On ”, the soft keyboard will
appear, input “7211”.

Figure 8-2 “Serve”interface Figure 8-3, input the password

3. In the “Check” interface, click “valve” to enter sample interface. Make the operation as
shown in figure 8-8 and 8-9.

Figure 8-8 “Check”interface Figure 8-9 “Valve”interface

4. As shown in figure 8-9, please first click “On” and then “Off”, you will hear the sound of “Ta”.
Then it shows the valve is working. Please note V12 does not exist since there are total 11
valves in the HL-3125Plus machine.

- 46 -
8.7 Piston Lubrication

1. In the“Main Menu”, click “Service”to“Serve”interface.

Figure 8-1 “Main Menu”

3. Click “Check” in serve interface. The password request window will pop-up. Make the
operation as the figure 8-2 and -3 as follows. Click “Key On ”, the soft keyboard will
appear, input “7211”.

Figure 8-2 “Serve”interface Figure 8-3, input the password

3. In the “Check” interface, click “sample” to enter sample interface. Make the operation as
shown in figure 8-8 and 8-10.

- 47 -
 Figure 8-8 “Check”interface Figure 8-10 “sample”interface
In the menu, there is an item "syringe down". You can input "8000" in the blank in front of
"syringe down". Click syringe down. Open the right panel, you can see the white piston and
please use the silicon oil provided in the accessories to lubricate the white piston.

The hole is used for use silicon oil, insert M2 screw

driver into it to roate the pistion to another side to
lubricate.

- 48 -
9. UNIT ADJUST

Enter the interface “service” from the main menu. Choose “Check” and input the service
password “72117211” to the service check interface as figure 9-1. Click “Adapter” as shown in
9-2. Here you can modify the unit of HCT and HGB.

Figure 9-1 Service Check interface

Figure 9-2 Adapter Setting

- 49 -
10. DATA TRANSMIT
RS232 Data Transmit Protocol
HL-3125Plus hematology analyzer adopt RS232 port to transmit the data. The RX, TX GND
total three data wires will be used. The connection method is as follows:
Computer DB9
HL Instrument male connector

RX 2 3 TX

TX 3 2 RX

GND 5 5 GND

Transmit protocol:
Baud rate: 115200
Character：8
Parity check bit：None
Stop bi：1
Flow control: None

Moreover, please select the printer + network option used in the “Print Setting Option”, you can
review the interface in detail and realize transmission and printing for historical data. The COM
port in PC receiver shall be set correctly.

2、Data Format
MYTHIC 1; VQQM;RESULT [CR]
DATE;dd/mm/yyyy [CR]
SID;Sample Identification [CR]
WBC;Value;;; [CR]
RBC;Value;;; [CR]
HGB;Value;;; [CR]
HCT;Value;;; [CR]
MCV;Value;;; [CR]
MCH;Value;;; [CR]
MCHC;Value;;; [CR]
RDW; Value;;; [CR]
PLT;Value;;; [CR]
MPV;Value;;; [CR]
THT;Value;;; [CR]
PDW;Value;;; [CR]
LYM%;Value;;; [CR]
MON%;Value;;; [CR]

- 50 -
 GRA%;Value;;; [CR]
LYM;Value;;; [CR]
MON;Value;;; [CR]
GRA;Value;;; [CR]
WBC CURVE; Channel value1;… ; Channel value 128 [CR]
WBC THRESHOLDS;S1;S2;S3;S4 [CR]
RBC CURVE; Channel value1;… ; Channel value 128 [CR]
RBC THRESHOLDS; S1;S2 [CR]
PLT CURVE; Channel value1;… ; Channel value 128 [CR]
PLT THRESHOLDS; S1;S2 [CR]
ALARMS; [CR]
INTERPRETIVE_WBC; [CR]
INTERPRETIVE_RBC; [CR]
INTERPRETIVE_PLT; [CR]
COMMENT;;
END_RESULT; 34996 [CR]

3、Data Example：
MYTHIC 1; VQQM;RESULT
DATE;18/03/2010
SID;2
WBC; 0.0;;;
RBC;0.00;;;
HGB; 0;;;
HCT;**.*;;;
MCV;**.*;;;
MCH;**.*;;;
MCHC;***;;;
RDW;**.*;;;
PLT; 0;;;
MPV;**.*;;;
THT;**.**;;;
PDW;**.*;;;
LYM;**.*;;;
MON;**.*;;;
GRA;**.*;;;
LYM;**.*;;;
MON;**.*;;;
GRA;**.*;;;
WBC
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0
WBC THRESHOLDS;43;53;9;123

- 51 -
RBC
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0
RBC THRESHOLDS;10;110
PLT
CURVE;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;
0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0;0
PLT THRESHOLDS;10;110
ALARMS;
INTERPRETIVE_WBC;
INTERPRETIVE_RBC
INTERPRETIVE_PLT;
COMMENT;;
END_RESULT;34996

- 52 -