AC 310 Service Manual-V1.0

AC 310 Hematology Analyzer
Service Manual
(V1.0, Issue in 2019.06)
Wheisman Medical Technology Co., Ltd

18th Bldg.,Wangtang Industrial Park, Xin'gao Rd., Xili, Nanshan District, Shenzhen 518055, P.R. China
Email: service@wheisman.com
Tel/Fax: +86 755 86542379
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AC 310 Service Manual
Major warning signs used for the analyzer
Caution
Biochemical hazard
Warning for laser
Warning for high voltage
Warning for puncture
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Contents
MAJOR WARNING SIGNS USED FOR THE ANALYZER ............................................... 2
CONTENTS ........................................................................................................ 3
CHAPTER1. ANALYZER INSTALLATION ................................................................. 5

1.1 Analyzer unpacking ....................................................................................................... 5
1.1.1 Unpacking steps ..................................................................................................................................... 5
1.1.2 Handling method ................................................................................................................................... 5
1.2 Installation and environmental requirements................................................................... 5
1.3 Space requirement ........................................................................................................ 6
1.4 Remove the auxiliary fixing structure for transportation ................................................... 6
1.5 Connect the reagents and waste barrel ........................................................................... 6
1.6 Power connection ......................................................................................................... 8
1.7 Startup......................................................................................................................... 8
1.8 Blank Test ..................................................................................................................... 8
CHAPTER 2. HARDWARE SYSTEM..................................................................... 10

2.1 Introduction of boards: ................................................................................................ 10
2.1.1 Introduction of industrial control board: ............................................................................................. 10
2.1.2 Introduction of interface board: .......................................................................................................... 10
2.1.3 Introduction of touch screen control board: ....................................................................................... 10
2.1.4 Introduction of DC-DC board: ...............................................................................................................11
2.1.5 Introduction of RFID Reader: ................................................................................................................11
2.1.6 Introduction of main control board: .....................................................................................................11
2.1.7 Introduction of CBC pre-amplifying board:.......................................................................................... 12
2.1.8 LED display board:................................................................................................................................ 13
2.1.9 Optocoupler detection board: ............................................................................................................. 13
2.2 Location of each board in the analyzer .......................................................................... 13
CHAPTER 3. SOFTWARE SYSTEM...................................................................... 16

3.1 Software architecture .................................................................................................. 16
3.2 Software functions of upper computer .......................................................................... 17
CHAPTER 4. FLUID PATH SYSTEM ..................................................................... 19

4.1 Schematic of fluid path ................................................................................................ 19
4.2 Composition of fluid path system.................................................................................. 19
4.2.1 Electromagnetic valve .......................................................................................................................... 19
4.2.2 Syringe ................................................................................................................................................. 19
4.2.3 Other auxiliary devices ........................................................................................................................ 20
4.3 Analyzer test procedures.............................................................................................. 20
4.3.1 CBC test procedure in whole blood mode ........................................................................................... 20
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4.3.2 CBC test procedure in pre-diluted mode ............................................................................................. 21

4.4 Instructions of CBC whole blood measurement process .................................................. 22
4.4.1 Sample aspiration by sampling probe (0~6S)....................................................................................... 22
4.4.2 Measurement procedure for Whole blood .......................................................................................... 22
4.5 Process instructions of time sequence for other measurements ...................................... 25
4.5.1 Process instructions of time sequence for pre-dilution measurement ............................................... 25
4.6 Functional descriptions of fluid path ............................................................................. 25
CHAPTER 5. MECHANICAL SYSTEM .................................................................. 28

5.1 Host structure............................................................................................................. 28
5.1.1 Main panels structure of the analyzer ................................................................................................. 28
CHAPTER 6. PARAMETER ADJUSTMENT SETTING............................................... 31

6.1 Gain adjustment ......................................................................................................... 31
6.1.1 Conditions for gain adjustment ........................................................................................................... 31
6.1.2 CBC gain adjustment ............................................................................................................................ 31
6.2 Adjustment of mechanical position ............................................................................... 31
6.2.1 Conditions for adjustment of mechanical position .............................................................................. 31
6.2.2 Mechanical debug................................................................................................................................ 32
6.3 Adjustment of sensor alarm value................................................................................. 33
6.3.1 Conditions for adjustment of sensor alarm value................................................................................ 33
6.3.2 Adjustment of sensor alarm value ....................................................................................................... 33
6.4 Test............................................................................................................................ 34
6.4.1 Functional test ..................................................................................................................................... 34
6.4.2 Performance test ................................................................................................................................. 35
CHAPTER 7. MAINTENANCE AND CORRECT ERROR ............................................ 38

7.1 Analyzer maintenance ................................................................................................. 38
7.1.1 Daily maintenance ............................................................................................................................... 38
7.1.2 Weekly maintenance ........................................................................................................................... 38
7.1.3 Monthly maintenance.......................................................................................................................... 38
7.1.4 Other maintenance .............................................................................................................................. 38
7.2 Fault handling ............................................................................................................. 39
7.2.1 Clinical alarm........................................................................................................................................ 39
7.2.2 Fault Alarm........................................................................................................................................... 42
CHAPTER 8. SYSTEM TROUBLE SHOOTING ........................................................ 52

8.1 Installation of operation system for upper machine ............................................................................. 52
8.2 Recover Application software. ............................................................................................................. 54
8.3 Analyzer IP settings ............................................................................................................................. 57
8.4 Main board IP capture tool introduction. ............................................................................................. 58
8.5 Application software update guide ...................................................................................................... 60
8.6 Some settings in windows system . ..................................................................................................... 61
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Chapter1. Analyzer installation
1.1 Analyzer unpacking

1.1.1 Unpacking steps
Unpack the analyzer, and properly store the packing case and the packing materials for convenience
of repacking analyzer in the future.
1. Keep the package upright, and make sure the package arrow up.
 Unpack the packing case with a tool, take out the accessories, and check whether the parts are
complete according to the packing list
2. Remove the instructions and the accessories from the buffering sponge, and keep them properly
for use in the future. Take out the upper buffering sponge, remove the damp-proof plastic bag cased
on the Analyzer, then lift the analyzer out of the packing case carefully by at least two persons who
hold the hand-lifting positions at the bottom on both sides of the analyzer, and place it on a
horizontal bench.
Notice: The packed accessories of the product shall be subject to the packing list.
1.1.2 Handling method

 Before handling, make sure to bind the sampling assembly reliably, in particular to ensure the
sampling probe in the topmost position.
 Under the condition of a short distance, use a trolley or other transport machines for
transportation.
 In all the processes of handling and transportation, pay attention to protect the front panel and
sampling probe, and keep the fluid path on the back panel from bearing external force,
contacting with other object, and being damaged.
 In all the processes of handling and transportation, the machine must be kept in the upright
direction, the inclination angle shall not be greater than 15°, and the machine can’t be placed
laterally.
 In handling, try to keep steady and avoid vibration, and firstly perform inspection and
debugging after handling before application.
1.2 Installation and environmental requirements

The analyzer must be installed by authorized personnel. In order to ensure the normal operation of
the analyzer, please select the working place meeting the requirements below to place the analyzer:
 Range of operating temperature range: 15 ℃~30 ℃
 Range of operating humidity range: 30%~85%
 Range of operating atmospheric pressure: 70 kPa~106 kPa
 The environment shall be free from dust, mechanical vibration, pollution, big noise source and
power interference as much as possible.
 It is recommended to evaluate the electromagnetic environment of the lab before operating
the equipment.
 Never get the equipment close to any strong electromagnetic interference source, so as to
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avoid the influence on the normal operation of the equipment.

 Never get close to any brush motor, flashing fluorescent lamp, and frequently on-off electric
contact equipment.
 Avoid direct sunshine or placing it in front of any heat source and wind source.
 Select a well-ventilated position.
 Never place the main machine on an inclined plane.
1.3 Space requirement

The reserved space between the right door of the main machine and the wall shall be ≥100cm, the
reserved space between the back panel of the main machine and the wall shall be≥50cm, and enough
space shall be ensured below the operating bench and the main machine for placing the buckets of
diluents and waste fluid (the space necessary for maintenance and service shall be ensured, the heat
radiation of the analyzer is considered, and the fluid pipelines behind the main machine are not
extruded to affect the normal flowing of reagents).
1.4 Remove the auxiliary fixing structure for transportation

a. Remove the fixed block of the movable mechanism on the sampling assembly;
b. Remove the tape on the Counting Chambers
1.5 Connect the reagents and waste barrel

1、 Connect the reagent and waste fluid pipes to the reagent bottle and the corresponding interfaces on
the back panel of AC 310 analyzer. The interfaces are as shown in the figure below.
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Picture of lower left interface on the back of the machine
Picture for connecting the machine side to reagent
Table 1-1 Reagent Interface Connection list
Interface Reagent Machine interface Interface color

1 Diluent Diluent Blue
2 Waste Waste Black
3 Waste sensor W. sensor
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4 Lyse W-31L Lyse Green

5 Probe cleanser Directly aspirate from the sampling
probe
1.6 Power connection

1. Connect the AC 310 analyzer with a patch board or socket with a power cord.
2. Connect the printer with AC 310(if use external printer).
1.7 Startup
After confirming that all the connections related to the analyzer are correct, firstly switch on the
power socket, and then turn on the power switch of the AC 310 analyzer. After the analyzer displays the
login interface, input the user name of “admin” and input the corresponding password which originally
is also ”admin”, press “OK” to enter the interface of waiting for connection, and enter the report
interface on completion of initiation, perfusion, cleaning and blank best.
1.8 Blank Test

1. Blank test
Click “ ” on the software interface, and select the blank test mode as shown in the figure
below:
Then press the count start key for blank test; if the blank fails to reach the standard, perform the blank
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test again; if it still fails to reach the standard, enter “ →clean” for “clean fluid ”, and then
perform the blank test again until the blank meets the requirements shown in the table below.
Table 1-2 Blank Indicators
Parameter Blank value
WBC ≤0.2ⅹ109/L
RBC ≤0.02ⅹ1012/L
PLT ≤10ⅹ109/L
HGB ≤1g/L
HCT ≤0.5%
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Chapter 2. Hardware system
From the view of system, the hardware system is between the software system and the mechanical
driving system of fluid path, and it plays a role as a connecting link. The ultimate user manages the
whole resources of the analyzer through the software of the upper computer. Specifically, the software
performs the related actions and data acquisition through the hardware.
2.1 Introduction of boards:

Based on the design principle, the boards of the hardware system classified into:
 Upper computer related boards: including industrial control board, interface board, touch
screen control board, DC-DC board and RFID reader;
 Middle computer related boards: main control board, CBC pre-amplifying board, and LED
display board;
 Lower computer related boards: 2 types of driver board and optocoupler detection board.
2.1.1 Introduction of industrial control board:

The industrial control board serves as the upper computer, and, it is used as the entire data
processing unit for externally connecting with the printer, mouse and keyboard, for mobile data storage,
etc;
2.1.2 Introduction of interface board:
According to the requirements for mechanical design, EMC design, appearance, etc., the purpose
for interface board design only satisfy the physical extension of interface for the industrial control board.
PCBA layout:
2.1.3 Introduction of touch screen control board:

Controlled by the touch screen; connect and communicate with the industrial control board
through the USB5 interface.
PCBA layout:
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2.1.4 Introduction of DC-DC board:

+24V switchover +12V power supply, provide independent power supply for the industrial control
board;
PCBA layout:
2.1.5 Introduction of RFID Reader (for close system):

If the Analyzer is a close system, it uses the RFID Reader to read the reagent card, and it connect
and communicate with the industrial control board through the USB6 interface;
2.1.6 Introduction of main control board:

The main control board as the middle computer of the system, is used as the action command
control and data acquisition center at the front end of the analyzer, and it downloads commands for the
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lower computers to execute;

PCBA layout:
2.1.7 Introduction of CBC pre-amplifying board:

Acquire WBC, RBC, PLT and HGB signals; adopt the manner of constant flow source;
PCBA layout:
J1
J3 J2
J4
Definition of interface signal:
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Position Name
J1 CBC signal pre-amplification interface
J2 WBC Apertures interface
J3 RBC Apertures interface
J4 HGB interface
2.1.8 LED display board:

LED display, the green light indicates power supply, the red light indicates warning, the yellow
light indicates running, and the buzzer gives audible warning;
2.1.9 Optocoupler detection board:

Detect whether the reagent fluid has bubbles or not;
PCBA layout:
Definition of interface signal:
Position Name Definition

J1 Detection of 1 Anode A at transmitting terminal
optocoupler 2 Cathode K at transmitting terminal
detecting signal 3 Collector C at receiving terminal
4 Emitter E at receiving terminal
2.2 Location of each board in the analyzer
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Pre-amplifying board
Interface board
Main Board
Lyse Sensor
Hard Disk
IPC board
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Touch panel
control board
LED display
Board
Touch screen
control board
14----5V switch power supply

15----DC-DC board
16----24V small switch power supply
17----Repositionable switch
18----EMI filter
19----Equipotential pole
20----24V big switch power supply
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Chapter 3. Software system
3.1 Software architecture
The software architecture is divided into four layers: upper computer, middle computer, master
lower computer and slave lower computer. The lower computers are mainly responsible for the control
of partial time sequences, the execution of control command sent by the upper computer, and the
driving control of mechanical components; they also have the functions of temperature and pressure
detection, etc. The middle computer realizes the functions of storing the time sequence files of the
analyzer, executing the control command sent by the upper computer, controlling partial time sequences,
acquiring signal data, etc. The upper computer is responsible for the operations of user interaction,
operation and control of analyzer running, result display, printing output, etc. Since only the upper
machine computer system is involved in the user interaction, no more instructions will be given for the
systems of the middle computer and the lower computers.
The software architecture is shown in the figure below:
Upper Middle Slave Lower

TCP/IP RS232 Lower Computer RS232
Computer Computer Computer
Figure: Software System Architecture Chart
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3.2 Software functions of upper computer
AC 610 software
AC310 Software
QC Report Calibration Setting WorkList Service Exit
L-J X-B X-AVG X-R Maintainece Self-Test Update
System Status Log Data Maintain
Manual Calibrator Fresh Blood History
Reference Print Dictionary Lab. Information Flag
Unit Option User Auto Maintain System Setting
Figure: Software Functions and Architecture Chart of Upper Computer
Classification of main functional modules:
Module name Functional description

Report Browse the test results, check, modify, print the results, etc.
Graph review Display the sample information in the form of graph and
sample details; edit the sample results and information;
check, delete and print the sample results. For open models,
sample counting can be started in this interface.
Quality control L-J quality The quality control product is used for
control quality monitoring of the analyzer, L-J
quality control has two modes of whole
blood and pre-dilution, and the operator is
allowed to perform quality control for all
the parameters or several selected
parameters.
X-B quality X-B quality control performs statistical
control analysis according to the actually tested
sample without using the quality control
product. The analyzer performs X-B quality
control for the three parameters of MCV,
MCH and MCHC, and both the whole blood
samples or pre-diluted samples can be used
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as the sample sources.

Quality Quality control of X mean value refers to
control of X that the mane value of two quality control
mean value tests is used as the final data for the
calculation of quality control.
Quality Quality control of X mean value R, namely
control of X the mean value – range quality control, is
mean value R an effective method for judging and
predicting whether the quality condition
has any abnormal fluctuation, and it is
complementary with L-J quality control.
Quality control of X mean value R uses the
quality control product for \quality control
analysis.
Calibration Manual The user can directly input the calibration
calibration parameters here.
Calibration The user calculates the calibration
using coefficient after several tests using the
calibrator calibrator
Calibration of The user calculates the calibration
fresh blood coefficient after several tests using multiple
fresh blood samples.
Service System maintenance, check of system status information, etc.
Setting Set the modifiable items related to daily operation and
analyzer maintenance.
Exit Exit from the system and execute the related operations of
analyzer shutdown.
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Chapter 4. Fluid path system
4.1 Schematic of fluid path
LH Lyse Diluent
SV9
WBC RBC
SV4
Press
SV10 Bath
SV3
2
1
SV6
Isolation
Isolation
SV2
SV1
SV8 SV7
ASP Syringe
DIL Syringe
SV12
Waste
Pump
SV11
4.2 Composition of fluid path system

The fluid path system is composed of 11 electromagnetic valves, 2 syringes, 2 counting chambers, 2
isolation chambers, 1 waste fluid pump, 1 pressure chamber, 1 sampling probe,etc.
4.2.1 Electromagnetic valve

Types of
Types of electromagnetic
electromagnetic Name of schematic Name of schematic
valves
valves
Three-way valve SV1 Two-way valve SV8
Three-way valve SV2 Three-way valve SV9
Three-way valve SV3 Two-way valve SV10
Three-way valve SV4 Three-way valve SV11
Two-way valve SV6 Three-way valve SV12
Two-way valve SV7
4.2.2 Syringe
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Syringe name Application

ASP syringe Sample aspiration and distribution
Quantitative fluid adding to the reaction chamber, quantitative sample adding
DIL syringe of various reagents, cleaning of related pipelines of the sampling probe, and
counting chamber. Sample preparation, assistance the sample moving
4.2.3 Other auxiliary devices

Name of diagram
Name of material Application
form
Discharge the waste fluid, and create positive and negative
Waste fluid pump PUMP1
pressures in the pressure chamber
Isolation chamber Isolation chamber Prevent foaming and backflow of waste fluid;
Air pressure sensor Air pressure sensor Monitor the air pressure in the pressure chamber
Level sensor Level sensor Detect the existence of reagent
Pressure chamber Pressure chamber Store positive pressure and negative pressure
4.3 Analyzer test procedures

4.3.1 CBC test procedure in whole blood mode
Aspirate 7.9ul of blood
Diluent 1940μl
Distribute 7μl of blood

to the WBC bath
Aspirate 42μl of blood
Mix WBC bath
Diluent
Lyse 300μl and 2858μl
Dilent 500ul
Mix WBC bath for Mix RBC bath
the second time
WBC counts RBC, PLT counts

HGB
measurement
Clean WBC Clean RBC
bath bath
Test end
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4.3.2 CBC test procedure in pre-diluted mode

External Analyzer operation
Capillary Blood 20μl
Diluent 200μl
Mix samples
Diluent
1719.73μl
Distribute 7.273μl of
blood to the WBC bath
Mix WBC bath
Diluent
Lyse 300μl and
2965μl
Dilent 500ul
Mix WBC bath for
Mix RBC bath
the second time
WBC counts
RBC, PLT counts
HGB measurement
Clean WBC Clean RBC

bath bath
Test end
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4.4 Instructions of CBC whole blood measurement process

4.4.1 Sample aspiration by sampling probe (0~6S)
The sample syringe aspirate 7.9ul of blood sample through the sampling probe.
The sampling probe is moved up, and the outer wall of the sampling probe is cleaned.
4.4.2 Measurement procedure for Whole blood
1、 Drain the RBC chamber: SV7、SV12 valve are open, and the pump drains the RBC chamber, see
the figure below.
WBC RBC
Press
SV10 Bath
2
1
Isolation
Isolation
SV8 SV7
SV12
Waste
Pump
SV11
Drain the RBC chamber
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WBC RBC
Press
SV10 Bath
2
1
Isolation
Isolation SV7
SV8
SV12
Waste
Pump
SV11
Drain the WBC chamber
2、 Measure the HGB background: 0.5S HGB performs background measurement.
3、 Drain the WBC chamber(6-8.8S): SV8、SV12 valve are open, and the pump drains the WBC
chamber, see the above figure.
4、 Create foaming positive pressure: SV11 is open, and the pump starts to create the pressure of
6.5KPA in the Pressure chamber
Press
SV10 Bath
2
1
SV8 SV7
SV12
Waste
Pump
SV11
Build Positive pressure
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5、 Add base solution to the WBC chamber: SV1, SV3 and SV4 valve are open, and the diluent
syringe injects a certain volume of base solution to the WBC chamber.
6、 Add blood to the WBC chamber: The sampling probe moves into the bottom of the WBC
chamber, SV1 and SV4 valves are open, and sample syringe injects the sample to the chamber; after
complete adding blood, the sampling probe moves to the upper position to clean the inner and
outer walls of the sampling probe; at the same time, SV8 valve open, and the sample is mixed by
bubbles.
7、 Create positive pressure: SV11 open, and the pump starts to create the pressure of 6.5KPA in
the Pressure chamber
8、 Aspirate the sample after dilution: After mixing, the sampling probe moves into the bottom of
the WBC chamber, the sample syringe aspirate a certain volume of sample, after sampling, the
sampling probe moves up, and clean the outer wall of the sampling probe.
9、 Aspirate the Lyse: SV1, SV3, SV4 and SV9 valves open, and the diluent syringe aspirate the
Lyse.
10、Add reagents to the WBC chamber (diluent and Lyse): SV1, SV3 and SV4 valves open, and the
diluent syringe injects a certain volume of diluent and Lyse to the WBC chamber; Then SV8 valve
open, and mix the blood samples in the WBC chamber by bubbles.
11、Add base solution to the RBC chamber: SV1, SV3 valves open, the diluent syringe injects a
certain volume of base solution to the RBC chamber.
12、Add the sample after dilution to the RBC chamber: The sampling probe moves into the RBC
chamber, SV1 and SV2 valves open, and the diluent syringe injects a certain volume of diluent for
dilution and sampling a certain volume from WBC chamber to the RBC chamber; the sampling
probe moves to the upper position, and clean the outer wall of the sampling probe at the same
time; and then SV8 valve open, mix the samples in the RBC chamber by bubbles.
13、Create negative pressure: SV11 and SV12 valves open, and the pump starts to create the
pressure of -24KPA in the pressure chamber.
14、Count the WBC and RBC/PLT: SV10 valve open, and it begins to count and measure the WBC
and RBC/PLT after the fluid flow becomes stable.
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WBC RBC
Press
SV10 Bath
2
1
SV6
Isolation
Isolation
SV8 SV7
SV12
Waste
Pump
SV11
15、Measure the HGB: At 1s before the WBC measurement is finished, it begins to measure the
value of HBG samples.
16、Clean the WBC chamber and RBC chamber: After blood tests, the WBC chamber and the RBC
chamber shall be cleaned thoroughly.
17、Add base solution to the WBC chamber and the RBC chamber: SV1, SV3 and SV4 valve open,
and the diluent syringe injects a certain volume of diluent as the base solution into the WBC
chamber; and then SV1 and SV3 valve open, and the diluent syringe injects a certain volume of
diluent as the base solution into the RBC chamber.
4.5 Process instructions of time sequence for other measurements

4.5.1 Process instructions of time sequence for pre-dilution measurement
The difference between the process of time sequence for pre-dilution measurement and the
process of time sequence for whole blood measurement is that: the measurement process of the time
sequence for pre-dilution measurement need to take diluent and mix 20ul blood, then aspirate to test.
4.6 Functional descriptions of fluid path
Functional names of
Functional descriptions Remark
Fluidics
Whole blood Detect all the parameters of the analyze
Pre-dilution Capillary Blood pre-dilution measurement for all parameters.

Peripheral whole Peripheral whole blood measurement for all parameters.
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blood
Used for providing diluent to dilute the Capillary Blood sample in
Diluent
the pre-dilution mode。
All the pipelines of the analyzer are perfused with the
Fluid path perfusion corresponding reagents, and the status for sample test can be
reached after completion.
Replace Lyse Replace the Lyse in the analyzer.
Replace diluent Replace the diluent in the analyzer.
Clean the WBC chamber using the diluent; foaming for
Clean WBC chamber
strengthened cleaning is available in the process of cleaning.
Clean the RBC chamber using the diluent; foaming for strengthened
Clean RBC chamber
cleaning is available in the process of cleaning.
Clean sampling probe Clean the inner and outer walls of the sampling probe.
Clean all the devices and pipelines in contact with the blood
Cleaning fluidics
samples in the analyzer using the diluent.
Drain WBC chamber Drain the WBC chamber.
Drain RBC chamber Drain the RBC chamber.
Drain all the fluid in the analyzer, including those in the devices and
Drain fluidics
in the pipelines.
Firstly, drain all the fluid in the analyzer, then perfuse the analyzer
Shutdown after
with purified water or deionized water, then drain the fluid in the
packaging
analyzer, and finally shut down the analyzer.
Drain the pressure
Eliminate all the fluid in the pressure chamber.
chamber
Back flush the WBC and RBC Apertures by high pressure at the
Flush Apertures
same time, so as to eliminate the pollutants.
Zap the WBC and RBC Apertures at the same time, so as to
Zap Apertures
eliminate the pollutants.
Unclog Apertures Combination of back flushing and burning
Startup for normal Startup cleaning process after the analyzer is shut down through
shutdown the “shutdown” function on the software
Startup cleaning process after the analyzer is shut down not
Startup for abnormal through the “shutdown” function on the software but by directly
shutdown powering off, etc.; the cleaning degree for this process is greater
than that for the startup after normal shutdown.
The startup cleaning for shutdown after packaging equals to adding
Startup after pack-up the function of fluid path perfusion before the cleaning procedure
for normal shutdown.
Shut down the analyzer software through the “shutdown” function
on the software; before shutting down the analyzer, the analyzer
Shutdown
executes the immersing process for each chamber and pipeline
using the probe cleanser.
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After the probe fluid enters the analyzer and then diluted, the
Sock Fluidics with devices and pipelines in contacted with blood samples shall be
probe Cleanser perfused, immersed and cleaned, the immersion shall last for 5
minutes, and the immersion cannot be canceled.
After the analyzer is not in used for longer time, recollect the
Enter the sleep sampling probe, and fill the WBC/RBC chamber with diluent for
immersion.
For the sleep time is less than 1 hour after sleep, when exiting from
Exit from sleep 1 (less sleep, maintain the fluid path mainly by flushing each chamber and
than 1 hour) pipeline, in order to ensure the measurement accuracy of the
analyzer.
For the sleep time is more than 1 hour and less than 5 hours after
Exit from sleep 1
sleep, when exiting from sleep, maintain the fluid path mainly by
(greater than 1 hour,
flushing each chamber and pipeline, in order to ensure the
less than 5 hours)
measurement accuracy of the analyzer.
For the sleep time is more than 5 hours after sleep, when exiting
Exit from sleep 3
from sleep, maintain the fluid path mainly by flushing each
(greater than or
chamber and pipeline, in order to ensure the measurement
equal to 5 hours)
accuracy of the analyzer.
Positive pressure
Inspect whether the process of creating positive pressure is normal
self-inspection
Vacuum pressure
Inspect whether the process of creating vacuum is normal
self-inspection
Pressure release Release the pressure in the pressure chamber and the pipelines
Recover all the fluid path devices to the prepared status for
Initialize Fluidics
measurement
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Chapter 5. Mechanical system
5.1 Host structure

5.1.1 Main panels structure of the analyzer
Touch Screen
Machine status indicator

Lyse door
Aspirate Key
AC 310 structure (front view)
USB port
LAN port
Fan
Waste sensor
Power Switch
Waste
Power socket
Diluent
Grounding rod
AC 310 structure (back view)
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Sampling probe
assembly
Sample syringe
Swa
b
Diluent Syringe
Sampling Probe
AC 310 structure with the face shell removed
Diluent Sensor
Pressure Chamber
WBC chamber
RBC chamber
Isolator Waste Pump
AC 310 structure with the right side plate removed
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Pre-amplifying board
Interface board
Main board
Hard Disc
IPC board
Power Supply
Lyse sensor
AC 310 structure with left side plate removed
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Chapter 6. Parameter adjustment setting
6.1 Gain adjustment

6.1.1 Conditions for gain adjustment
1、 Replace the WBC or RBC chamber.
2、 Replace the Apertures.
3、 Replace the CBC board.
4、 Replace the main control board.
6.1.2 CBC gain adjustment
Enter the interface of “ ” →“Service” → “Debug”→ “Basic parameter”.
Perform the sample analysis using the normal control in this interface for 3 times successively, and
calculate the mean values of “NEU peak” and “RBC peak”, respectively; the mean values of “NEU peak”
and “RBC peak” are required to be within the ranges of 265±3 and 85±1.5, respectively, and “HGB
blank” is required to range from 2800 to 3000, if:
 the “NEU peak” is greater, adjust to reduce the “WBC gain” value; the “NEU peak” is smaller,
adjust to increase the “WBC gain” value.
 the “RBC peak” is greater, adjust to reduce the “RBC gain” value; the “RBC peak” is smaller, adjust
to increase the “RBC gain” value.
 the “HGB blank ” is greater, adjust to reduce the “HGB Gain” value; the “HGB blank AD” is smaller,
adjust to increase the “HGB Gain” value.
6.2 Adjustment of mechanical position

6.2.1 Conditions for adjustment of mechanical position
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1、 The sampling assembly has been moved and replaced.

2、 The position optocoupler in the horizontal or vertical direction on the sampling assembly has
been replaced.
3、 The reaction chamber assembly has been replaced: WBC/RBC reaction chamber assembly.
6.2.2 Mechanical debug
Enter “ → Service →Debug → Machinery” to confirm each position of the sampling probe.
【1】 The Horizontal Position of Sample is fixed with 0, Vertical Position of Origin is fixed with 0 ,Make
Bubble Motor step 100, Wbc/Rbc Depth 296.
【2】 In the process of position detection, it is prohibited to click the “Get Steps” button at random,
otherwise it will lead to the problem of error in pointer position.
【3】 Please perform “Initialize” before adjustment, and make sure to perform “Initialize” after
detecting each position.
【4】 Firstly, detect whether the horizontal position is correct; taking the detection of “WBC Cell”
position as an example, click the “Confirm Pos.” button under the “WBC Cell” position, and then
judge whether the sampling probe assembly is moved to the “WBC Cell” position; the standard
for judgment is that: click the “VM Disable” button, and then manually move the sampling
probe down until the sampling probe is inserted into the WBC chamber; the sampling probe is
required to be near the middle position of the chamber in the horizontal direction; if the
deviation is greater, firstly inspect whether the horizontal belt is loose; if yes, adjust the
horizontal belt, and then perform the detection; Make sure to perform “Initialize” before the
next detection. If it is not the problem of looseness in the horizontal belt, it is necessary to
adjust the probe position according to the following methods:
Click “HM Disable” and “VM Disable”, manually move the movable needle frame horizontally to the WBC
chamber position, then insert the sampling probe into the WBC chamber for about 30mm, adjust the
movable needle frame to make the sampling probe in the middle position at the port of WBC chamber
in the horizontal direction, and then press the “Get Steps” button corresponding to the “WBC Cell”
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position in the horizontal position; on completion, the steps of the WBC chamber in the horizontal
direction will appear in the input field of the WBC chamber position; click the “Setup” button to finish
the adjustment. On completion of position adjustment, perform confirmation detection for the
debugging results, and see steps [3] and [4] for the methods.
【5】 The detection steps for other positions are the same as [4].
【6】 For detection in the vertical direction, the standard for judging whether it is in the correct
direction is that: the distance from the tip of the sampling probe to the bottom of each chamber
is about 2mm. if the deviation is greater, adjust the needle position in the vertical direction;
taking the position of WBC chamber in the vertical direction as an example according to the
following methods: click “HM Disable” and “VM Disable”, manually move the movable needle
frame horizontally to the WBC chamber position, then insert the sampling probe into the WBC
chamber for about 30mm, adjust the movable needle frame to make the sampling probe at
about 1mm on the right of the middle position at the port of WBC chamber in the horizontal
direction, insert the sampling probe to the bottom of the WBC chamber (just contact with the
chamber bottom), and then click the “Get Steps” button corresponding to the “WBC Cell”
position in the vertical position; on completion, the steps of the WBC chamber in the vertical
direction will appear in the input field of the WBC chamber position, and the steps shall be
rewritten in the input field after subtracting the steps by 16; click the “Setup” button to finish
the adjustment.
【7】 The sampling position in the vertical direction shall be confirmed according to the following
method: it is required to extend the sampling probe from the swab for 91~92mm under the
condition that the swab closely adheres to the lower edge of the mounting position, if it is not
within the range, it can be adjusted by increasing or reducing the steps in the position.
6.3 Adjustment of sensor alarm value

6.3.1 Conditions for adjustment of sensor alarm value
1、 The analyzer always alarms for a certain or several sensor parameters.
2、 Replace the sensor.
3、 The pipeline in the sensor has been removed.
6.3.2 Adjustment of sensor alarm value
Enter the interface of “ → Service →Debug→ Sensor”.
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1、 Take the connecting pipelines of all reagents out of the respective reagent bottle or bucket,
enter interface of reagent replacement to replace each reagent, drain the reagents in the
pipelines in the diluent sensor and the Lyse sensor, and then input the value by adding 50 to the
readings corresponding to the “current value” in the input fields behind diluent deficient and
Lyse deficient.
2、 Click the “Setup” button to save.
3、 If a single sensor is involved, it is only necessary to debug the corresponding parameters.
6.4 Test
6.4.1 Functional test
1. Enter “ →Service →Self-test”, as shown in the figure below:
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Detection requirements:
 The devices must be detected in turn, and it is not allowed to detect the next device before
finishing the detection of the former device.
 Each device shall be in steady running without stagnation and abnormal noise.
 For the detection of valves, 11 sounds for opening and closing the valves shall be heard.
 No alarm is allowed for the detections of positive pressure and vacuum.
2. Grounding inspection
Use the 200  gear of the multimeter to test whether the resistance between all screws of the back
plate and the grounding terminal of the power supply is less than 0.5  ; it is unqualified if it is greater
than 0.5  .
3. Inspection of software version
Inspect whether the versions of the upper computer software, middle computer software, driver
board software, fluid path time sequence and FPGA are conforming with BOM; it is unqualified if they
are not conforming.
4. Inspection of waste fluid alarm function
In the testing process, when the floating ball on the waste fluid sensor is pushed to the upper part,
the analyzer interface will prompt for full waste fluid; it is unqualified if the prompt is not heard.
5. Inspection of prompt tone and alarm sound
In the report interface, press the suction key for normal test; when the sampling probe is moved up
on completion of sample suction, there will be voice prompt, and it is unqualified if the voice prompt is
not heard.
6.4.2 Performance test
1. Blank counting: In the report interface, in the CBC whole blood mode, it is required to perform blank
counting for 3 times successively, and the results shall all meet: WBC≤0.2, RBC≤0.02, PLT≤10 and HGB≦1;
if it still fails to meet the requirements, it is necessary to inspect whether the reagent is polluted, and
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the analyzer shall be cleaned and maintained; if it still fails to meet the requirements, it will be
processed according to the processing for faulty machine;
2. Test of carried contamination: In the report interface, in the CBC whole blood mode, firstly perform
the test with high-value quality control for 3 times, then perform the blank test for 3 times, and record
the test results; calculate the rate of carried contamination according to the results. The rate of carried
contamination is required to be: WBC≦0.5%, RBC≦0.5%, PLT≦1% and HGB≦0.5%. Note: Requirements for
high-value sample: WBC＞15, RBC＞6, PLT＞200 and HGB＞300; low-value sample is blank test.
3. Repeatability test: In the report interface, select the CBC whole blood mode, perform the test using
more than 1mL of normal fresh human blood for 10 times successively, and calculate the CV value of the
10 test results. Test 3 human blood samples successively; the CV value of each blood sample is required
to meet: WBC≦2% RBC≦1.5% PLT≦4% HGB≦1.5% MCV≦1%.
4. Analyzer calibration: Use more than 2mL of normal control blood, perform the test for 3 times on the
standard machine, calculate the mean values of WBC, RBC, HGB, PLT, MCV and MPV as the target values
for quality control, respectively; or use the fresh blood samples which have been tested on other blood
cell analyzer and printed with reports, and take the test results on the reports as the target values.
①Calibration in whole blood mode: In the interface of fresh blood calibration, select the whole blood
mode, input the target values of the normal control blood in WBC, RBC, HGB, PLT, and MCV, and perform
the test on the machine using normal control (or fresh blood samples) for at least 3 times successively;
the CV value of the test results must meet: WBC≦2% RBC≦1.5% PLT≦4% HGB≦1.5% MCV≦1%,
and the results are saved; if the CV value of the test results cannot meet the requirements, it is
necessary to re-calibrate.
② Calibration in pre-dilution mode: In the interface of fresh blood calibration, select the pre-dilution
mode, input the target values of the normal control blood (or fresh blood sample) in WBC, RBC, HGB, PLT,
and MCV; press “Add diluent”, place the test tube under the sampling needle, press the suction key for 4
times to add the diluent by the analyzer, take 80uL of blood sample using the 100uL pipette, wipe off the
outer wall of the pipette head, then add the blood sample into the test tube which has been added with
the diluent, perform the test after fully mixing, and repeat the test for at lest 3 times; the CV value of the
test results must meet: WBC≦2% RBC≦1.5% PLT≦4% HGB≦1.5% MCV≦1%, and the results are
saved; if the CV value of the test results cannot meet the requirements, it is necessary to re-calibrate.
5. Test of analyzer comparability: Perform the test using more than 1mL of normal control blood (or
fresh blood) on the standard machine for 5 times, and calculate the mean value of each item as the
reference value.
① In the test interface, select the CBC whole blood mode, test the sample for 3 times, and calculate the
mean value of each item; the percentage of deviation between the mean value and the reference value
for each item shall meet: WBC±3% RBC±2% HGB±2% PLT±5% MCV±2%。
② In the test interface, select the CBC pre-dilution mode, click “OK” and then press “Add diluent”, place
the test tube under the sampling needle, press the suction key for 4 times to add the diluent by the
analyzer, take 80uL of the sample using the 100uL pipette, wipe off the outer wall of the pipette head,
then add the sample into the test tube which has been added with the diluent, perform the test on the
machine after fully mixing, test the sample for 3 times, and calculate the mean value of each item; the
percentage of deviation between the mean value and the reference value for each item shall meet:
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WBC±3% RBC±2% HGB±2% PLT±5% MCV±2%。
Page 37 of 65
Chapter 7. Maintenance and Correct error
7.1 Analyzer maintenance

7.1.1 Daily maintenance
* If the user leaves the analyzer switched on for 24 hours, the analyzer interface will automatically
perform the action of " Start clean with probe cleanser?" for one time every 24 hours.
* When the number of sample tests is up to 200 (the number can be set), the analyzer interface will
automatically perform the action of " Start clean with probe cleanser?".
7.1.2 Weekly maintenance
*When the number of samples tested is up to 600 (the number can be set) or at 1 week (7 days) interval,
the analyzer will automatically perform the action of " Start probe cleanser soak?
7.1.3 Monthly maintenance
* Wipe the surface of the sampling needle (especially the tip position) with a soft, dustless tissue every
month to clean up the stubborn stains on the surface of the sampling needle.
* Clean the blood stains on surface of the swab, especially the blood stains on the lower surface.
7.1.4 Other maintenance
*When the syringe operation makes an abnormal sound, its screw rod should be added lubricating oil.
*Every six months, check the horizontal and vertical belt of the lower sampling components is loose or
not, if loose, tension it again.
*If necessary, according to the different fault conditions, perform different maintenance under the
"Routine Maintenance" interface, to achieve the purpose of correct erroring.
1. When there is slight pollution, you can choose to use all cleaning functions under the "Clean"
interface.
Cleaning function
2. When there is serious pollution, you can choose to use the various functions under the
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"Maintain" and “Maintain the whole Device” interface.
Maintenance function
*When the analyzer is moved or transported for long distances or unused for more than 6 days, perform
the "Pack-up" action under the "Service - Maintain the whole Device" menu to complete the cleaning
and emptying of the analyzer fluid circuit and place it at a clean place.
7.2 Fault handling

7.2.1 Clinical alarm
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7.2.1.1 Alarm sign
7.2.1.1.1 Parameter fails to have results

If the parameter fails to have results after the test is completed, but displays a specific sign:
Table 1 Parameter Value Replaced Sign
Sign Description
Parameter is invalid or cannot be
---
calculated
+++ Parameter is beyond the display range
7.2.1.1.2 Parameter is accompanied with alarm sign
Parameter value alarm sign appears with the parameter result, whose specific meaning is as shown
in following table.
Table 2 Parameter Value Alarm Sign
Sign Description
+ Parameter is beyond the measurement range
Parameter suspicious sign represents that the reliability of the parameter result
is not high. Specific to all parameters
Parameter Possible reasons
1. Poor hemolysis
2. Erythrocytes, giant platelet and platelet aggregation
WBC interference
3. Electrical noise, bubble interference
4. Hole blockage
1. Analyzer is uncalibrated
HGB 2. High leukocyte interference and lipemia sample
* 3. Abnormal HGB voltage
1. Giant platelet / platelet aggregation interference
2. Microcyte is interfered by platelet
RBC 3. Abnormal distribution of erythrocyte
5. Hole blockage
1. Giant platelet / platelet aggregation
2. Microcyte
PLT 3. Abnormal distribution of platelet
5. Hole blockage
::: Hole blockage
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7.2.1.1.3 Alarm prompt message
Alarm prompt message is the explain information of the alarm result, whose specific content is
shown as the following table:
Table 3 Alarm Prompt Message
Type Alarm prompt message Possible reasons

Abnormal leukocyte 1. Abnormal sample
scatter diagram 2. Dirty or blocked flow cell
1. Poor hemolysis
Abnormal leukocyte
2. Signal noise, bubble interference
column diagram
3. Hole blockage
Leukocytosis WBC > set value
WBC Flag
Leukopenia WBC < set value
Neutrophilia NEU#> set value
Neutropenia NEU#< set value
Lymphocytosis LYM# > set value
Lymphopenia LYM#< set value
Mononucleosis MON# > set value
interference.
Abnormal erythrocyte
2. Microcyte is interfered by platelet.
distribution
3. Electrical noise, bubble interference.
4. Hole blockage.
Abnormal sample, e.g., the patient is under
Erythrocyte bimodality iron missing treatment, blood transfusion,
etc.
Polycythemia RBC > set value
RBC Flag Anemia HGB < set value
Uneven sizes of
RDW-SD/RDW-CV > set value
erythrocytes
Large cell erythrocyte MCV > set value
Small cell erythrocyte MCV < set value
Hypochromic
MCHC < set value
erythrocyte
Erythrocyte aggregation Abnormal RBC channel parameters
HGB interference? Abnormal RBC channel parameters
Iron missing Abnormal RBC channel parameters
Abnormal distribution 2. Microcyte
PLT Flag
of platelet 3. Signal noise, bubble interference
4. Hole blockage
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Thrombocythemia PLT > set value

Thrombocytopenia PLT ＜ set value
Abnormal PLT column diagram. If the
Platelet aggregation? distribution is wide, the tail of column
diagram is high.
7.2.2 Fault Alarm

7.2.2.1 Fault Alarm Information Overview
Fault code Fault name Fault code Fault name

258 Communication error! 1903 Manipulator horizontal Home error!
The model of analyzer is
1502 1904 Manipulator horizontal is not initialized!
abnormal!
The count times of analyzer
1503 1905 Manipulator vertical is not homed!
is abnormal!
Time sequence is not
1663 1906 Manipulator vertical Steps error!
founded!
Adjusting pressure chamber
1733 1907 Manipulator vertical Home error!
pressure failed!
1741 No Diluent! 1908 Manipulator vertical is not initialized!
1743 No Lyse! 1909 Sample syringe Steps error!
1745 Waste Full! 1910 Sample syringe Home error!
1747 Flow cell clog! 1911 Sample syringe is not initialized!
1748 Flow cell leaks 2003 Diluent syringe Steps error!
1749 Fluidics leaks, Press lower! 2004 Diluent syringe Home error!
1751 Drain pipe leaks 2005 Diluent syringe is not initialized!
1755 Diluent is expired 2101 Command is timeout!
1757 Lyse is expired 3201 Background test failed!
1760 WBC cell clog! 3202 Initialization failed!
The parameter of control board differ from the
1761 RBC cell clog! 6001
parameter file !
Ambient temperature is The parameter of control board differ from the
1770 6002
abnormal! parameter file !
Manipulator horizontal
1902
Steps error!
7.2.2.2 Fault alarm information handling
Fault code Fault name Fault cause Fault handling procedure

Communication command 1. Click the 'Correct Error' button to remove this
Communicatio conflict, command error.
258
n error! timeout, unidentifiable 2. Perform the shutdown procedure to shut down the
command analyzer and then restart it.
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3. If the error still exists, please check or change the

relevant board card.
The analyzer model,

analyzer serial number and
The model of 1、 Please use the original board card and original
other analyzer information
1502 analyzer is software.
saved by upper software,
abnormal!
middle software, lower
software are inconsistent
Special use for closed
The count
reagent system: the times
times of 1. Please use the original board card and original
1503 of the reagents use is
analyzer is software
different in upper software
abnormal!
and lower software
Time
Time series software is not
1663 sequence is 1. Please use the original board card.
installed in the analyzer
not founded!
1. Click "Correct error" button to perform
component initialization.
After finishing build-up of
Adjusting 2. If the fault still exists, please check whether the
pressure in pressure
pressure pipeline connected with the pressure chamber has
chamber, the pressure does
1733 chamber separation problem.
not meet the requirement
pressure 3. Check whether the liquid pump has abnormality.
or exceed the required
failed! 4. Check the air pressure detection relevant parts of
pressure
the driver board.
5. check SV11 and SV12 valve;
1. Click "Correct error" button to perform
During pressure detection, component initialization.
The pressure
the built-up of pressure 2. If the fault still exists, check whether the liquid
adjustment in
1734 inside the pressure pump has abnormality.
the pressure
chamber exceeds the 3. Check the air pressure detection relevant parts of
chamber fails
required highest limit. the driver board.
4. Check SV11 and SV12 valve;
1. Without reagent 1. Check whether the alarm information
2. Liquid level sensor corresponding reagent bottle has reagent. If there is
1741 No Diluent!
failure (pipeline bubble, no reagent, please change for another bottle of new
reagent missing alarm reagent. Then click "Correct error" button to perform
Page 43 of 65
value setting is not the action of changing reagent.

appropriate). 2. If the reagent is checked to have the much
3. Drive board failure remaining amount, please check whether part of the
pipelines of the level sensor of the alarm information
corresponding reagent have bubbles.
If have bubbles, find out where these bubbles
1743 No Lyse! caused, and remove the leakage.
if without bubbles, enter "Service→debug→Sensor"
interface, and check whether the reagent missing
alarm value is set properly. See Section 6.3 for
details.
3. Check the driver board level sensor detection
related parts and connections.
1. Replace new waste bucket or empty the waste.
2. If the waste bucket is not full, use a multimeter to
1. Waste bucket is full measure the waste sensor (NC type), if the circuit is
2. Waste sensor line or measured to be closed when the float is in the
1745 Waste Full!
sensor is broken. bottom and the circuit is cut off when the float is at
3. Drive board failure the top, the waste sensor is normal; if not, check
whether the sensor line or the sensor is damaged.
3. Detect the relevant part of the drive plate.
1. Check whether the solenoid valve SV7, SV8, SV10,
SV11, SV12 interface related pipelines are fall off.
1. Pipeline fall-off
2. If the pipeline connection is normal, please check
2. Solenoid valve failure
1749 Fluidics leaks! whether the solenoid valve SV7, SV8, SV10, SV11,
3.Pressure sensor or
SV12 are normal.
driver board failure.
3. If the fault still exists, replace with new hydraulic
sensor or check the relevant parts of the drive plate.
1. Check whether the solenoid valve SV7, SV8, SV11,
SV12 interface related pipelines are fall off.
2. If the pipeline connection is normal, please check
Drain pipe 2. Solenoid valve failure
1751 whether the solenoid valve SV7, SV8, SV11, SV12 are
leaks 3. Pressure sensor or
normal.
driver board failure.
3. If the fault still exists, replace with new hydraulic
sensor or check the relevant parts of the drive plate.
1、 Check whether the reagent has expired. If
Diluents is expired, please replace it, click the "Remove"
1755 1、 The reagent is out of
expired button to perform the replacement reagent.
period of validity.
2、 If the reagent does not expire, enter "Routine
2、 There is an error in
Maintenance - Replace Reagent", choose to
the period of validity
replace the appropriate reagent, and click
1757 Lyse is expired inputted.
"Set" to set the correct expiration date of the
reagent.
Page 44 of 65
1, click "Correct error" button to perform the action

of removing blockage.
2. Perform "Flush Apertures" blockage, observe
1760 WBC cell clog!
whether there is liquid shot to the inner wall on the
opposite side of the counting cup front chamber
through the chamber Apertures during the recoil
1. Apertures blockage process, if not, continue to perform "unclog
2. Apertures has bubble. Apertures" action until the Apertures is got through.
3. If perform the “Unclog Apertures” command and
the Apertures cannot be got through, please drain
1761 RBC cell clog!
the chambers ,and use syringe aspirate probe
cleanser and add into the chambers. And soak the
chambers about 10 minutes.
4. If the fault still exists, then replace the Apertures.
1. Check the analyzer using environment
1. The ambient
temperature is not in the temperature is within the [15-30] ℃ range, if
scope of use of the
Ambient beyond the normal range, the test results may not be
analyzer.
1770 temperature accurate.
2. The ambient
is abnormal! 2. If the ambient temperature is within the normal
temperature sensor is
range, check whether the ambient temperature
damaged.
sensor is normal.
3. Main board failure
3. Check the relevant part of the main board.
1. The detection 1. Click "Correct error" button to perform component
optocoupler in the initialization.
horizontal direction has 2. Check whether the sampling component level
Manipulator failure. optocoupler detection part is normal.
1902 horizontal 2. The sampling 3. Check whether the sampling component level
Steps error! component in the motor drive and mechanical transmission part is
horizontal direction normal.
transmission has failure. 4. Check whether the relevant part of the drive board
3. Main board failure is normal.
1. Click "Correct error" button to perform component
1. During the reset process, initialization.
Manipulator through the maximum 2. Check whether the sampling component level
1903 horizontal number of steps, the optocoupler detection part is normal.
Home error! optocoupler is still cannot 3. Check whether the sampling component level
sense the catch. motor drive and mechanical transmission part is
normal.
1. In the absence of initial
Manipulator action, let the sample
1904 horizontal is needle directly conduct the
initialization.
not initialized! action in the horizontal
direction.
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1. Before the sampling

Manipulator needle running in the
1905 vertical is not horizontal direction, there
initialization.
homed! is no reset to the vertical
direction.
1. The detection
initialization.
optocoupler in the vertical
2. Check whether the sampling component detection
direction has failure.
Manipulator optocoupler in the vertical direction is normal.
2. The sampling
1906 vertical Steps 3. Check whether the motor drive of the sampling
component in the vertical
error! component in the vertical direction and mechanical
direction transmission has
transmission part is normal.
failure.
4. Check whether the relevant part of the drive board
3. Main board failure
is normal.
1. During the reset process, initialization.
Manipulator through the maximum 2. Check whether the sampling component vertical
1907 vertical Home number of steps, the detection optocoupler is normal.
error! optocoupler is still cannot 3. Check whether the sampling component vertical
sense the catch. motor drive and mechanical transmission part is
normal.
1. In the absence of initial
Manipulator action, let the sample
1908 vertical is not needle directly conduct the
initialization.
initialized! action in the vertical
direction.
initialization.
1. Syringe detection
2. Check the syringe position detection optocoupler
optocoupler detection
Sample is normal.
failure.
1909 syringe Steps 3. Check whether the motor drive of the sampling
2. Syringe transmission
failure.
3. Drive board failure
is normal.
initialization.
When the syringe is reset,
Sample is normal.
the reset process is not
1910 syringe Home 3. Check whether the motor drive of the sampling
completed within the
specified number of steps
is normal.
Page 46 of 65
The syringe does not

Sample
perform an initialized 1. Click "Correct error" button to perform component
1911 syringe is not
action before the initialization.
initialized!
movement.
1. Syringe detection initialization.
optocoupler detection 2. Check the syringe position detection optocoupler
Diluent failure. is normal.
2003 syringe Steps 2. Syringe transmission 3. Check whether the motor drive of the sampling
error! failure. component in the vertical direction and mechanical
3. Main board failure transmission part is normal.
4. Check whether the relevant part of the main board
is normal.
initialization.
When the syringe is reset,
Diluent is normal.
the reset process is not
2004 syringe Home 3. Check whether the motor drive of the sampling
completed within the
specified number of steps.
4. Check whether the relevant part of the main board
is normal.
The syringe does not
Diluent
perform an initialized 1. Click "Correct error" button to perform component
2005 syringe is not
action before the initialization.
initialized!
movement.
1. Click "Correct error" button, and conduct blank
test again.
1. The results of blank test 2. Check whether the reagent is expired or
is beyond the provisions of contaminated.
the requirements. 3. Enter "Service→Maintenance→Clean" interface,
2. Counting tank is perform "Clean Fluidics" action, and re-test blank
contaminated or liquid after the operation.
Background
3201 circuit system has 4. Enter "Service→Maintenance→Maintain"
test failed!
bubbles. interface, perform "Unclog Apertures" action, and
3. Dilution is contaminated confirm the Apertures is not blocked and re-test
or expired. the blank.
4. Apertures blockage 5. Use the corresponding probe liquid cleaning
5. Noise interference. function to clean the corresponding parts.
6. Ensure that the analyzer is away from
interference sources.
Initialization
3201
failed!
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1、 Click "Correct error" button, select the

appropriate treatment according to different
circumstances:
The
1） When the replacement of the main control
parameter of The parameters of the
board causes the failure, choose to download
control board main control board and the
6001 parameters from the upper software software
differs from upper software software
to the main control board.
the parameter are inconsistent.
2） When upper software software re-installation
file!
or change causes failure, choose to download
parameters from the main control board to
the upper software software
2、 Click "Correct error" button, select the
appropriate treatment according to different
circumstances:
The
3） When the replacement of the main board
parameter of The parameters of the
causes the failure, choose to download
control board driver board and the upper
6002 parameters from the upper software software
differs from software software are
to the main board.
the parameter inconsistent.
4） When upper software software re-installation
file!
or change causes failure, choose to download
parameters from the main board to the upper
software software
Liquid
Order Possible
Circuit Solutions
Number reasons
failure
1. Check the pipeline from the swab to the liquid pump
through SV11 is connected normally and whether there
1. Connecting is leakage.
pipe is not 2. Enter "Service→Self-test" interface, and check whether
Liquid falls connected. SV11 valve can work normally.
1 out of the 2. SV11 valve 3. Enter "Service→Self-test" interface, and check the pump; if
swab failure. the pump cannot work, replace the pump. If the pump
3. Pump failure. can operate, open the pump head, check whether there
4. Swab wear. are particles or debris in the pump head diaphragm and
other positions; if so, clear them and re-test.
4. If the fault still exists, replace the swab.
1. Enter "Service→Self-test" interface, and check whether
1.SV8, SV11,SV12
SV8, SV11, SV12 valve, especially SV8 valve are working
valve failure.
WBC chamber properly.
2 2. Pump failure.
liquid overflow 2. Enter "Service→Self-test" interface, and check the
3.Drain pipeline
pump; if the pump cannot work, replace the pump. If
is detached.
the pump can operate, open the pump head, check
Page 48 of 65
whether there are particles or debris in the pump head

diaphragm and other positions; if so, clear them and
re-test.
1. Enter "Service→Self-test" interface, and check whether

SV7, SV11, SV12 valve, especially SV7 valve are working
1.SV7,SV11,SV12 properly.
2. Enter "Service→Self-test" interface, and check the
valve failure.
RBC chamber
4 2. Pump failure. pump; if the pump cannot work, replace the pump. If
liquid overflow
3.Drain pipeline the pump can operate, open the pump head, check
is detached. whether there are particles or debris in the pump head
diaphragm and other positions; if so, clear them and
re-test.
1. Enter "Service→Self-test" interface, and check the pump; if
the pump cannot work, replace the pump. If the pump
can operate, open the pump head, check whether there
are particles or debris in the pump head diaphragm and
other positions; if so, clear them and re-test.
2. Enter "Service→Self-test" interface, check valve SV11 and
two chambers corresponding discharge valve SV7, SV8
1. Pump failure.
are working properly.
2. Valve failure.
3. Enter "Service→Self-test" interface, click "Positive Pressure
Test", if there is alarm prompt, check whether the
There is no 4. Pipeline
5 pipeline connected with the valve SV7, SV8, SV10, SV11,
bubble coming blockage.
SV12 is detached.
out when 5. Pressure
4. Check whether the bubble pipeline (pipelines, all isolation
blending well. sensor
chamber and the corresponding valves SV7, SV8
failure.
between all reaction chambers and corresponding
isolation chambers) is filled with liquid.
5. Check whether the bubble pressure is within the range of
"3-7Kpa", if out of range, enter
"Service→Debug→Pressure" interface, and re-calibrate
the pressure.
6. Replace the main board, and re-calibrate the pressure.
1. Samples are
not well
1. Shake the sample well.
mixed.
2. Select the appropriate sample.
Most 2. Sample
3. Enter "Service→Maintenance→Clean" interface, and
indicators selection is
8 perform "Clean fluidics" action, and if still not work, use
Poor not good
the probe liquid to clean the machine.
reproducibility (abnormal
4. Replace reagent in expiration date or without pollution.
blood).
5. Eliminate the interference source.
3. Counting tank
is
Page 49 of 65
contaminate
d or liquid
circuit
system has
bubbles.
4. Reagent is
contaminated or
expired.
5.Noise
interference.
1. Sample
selection is
bad (WBC
abnormal 1. Select the appropriate sample.
blood). 2. Enter "Service→Maintenance→Maintain" interface and
2. Apertures perform "Unclog Aperture" action.
blockage in 3. Enter "Service→Maintenance→Clean" interface and
WBC perform "Clean WBC chamber" action; if the vacuole
chamber overflows the cup mouth after blending, clean the inner
3. WBC chamber wall of WBC chamber with soft, dustless cloth manually.
Poor WBC pollution 4. Observe whether the bubble blending process of the WBC
9
repeatability 4. RBC chamber chamber is normal. If no bubble comes out, refer to the
without correct error method above "No Bubble Comes Out in
blending the Bubble Blending Process".
bubbles 5. Check whether the Lyse is expired, and whether the action
5. Lyse fault to add Lyse is correct.
6. WBC gain 1、 Enter "Service→Debug→Basic Parameters→CBC gain"
setting is interface and re-adjust WBC gain.
inappropriat 7. Eliminate the interference source.
e
7. Noise
interference.
1. Sample
1. Select the appropriate sample.
selection is
2. Enter "Service→Maintenance→Maintain" interface and
not good
perform "Unclog Aperture" action.
(RBC/PLT
3. Enter "Service→Maintenance→Clean" interface and
abnormal
perform "Clean RBC chamber" action.
RBC/PLT blood).
4. Observe whether the bubble blending process of the RBC
10 Poor 2. Apertures
chamber is normal. If no bubble comes out, refer to the
reproducibility blockage
correct error method above "No Bubble Comes Out in
3. RBC chamber
the Bubble Blending Process".
pollution
5. Enter "Service→Debug→Basic Parameter→CBC gain"
5. RBC chamber
interface and readjust WBC gain.
without
6. Eliminate the interference source.
bubbles
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4. RBC gain
setting is not
appropriate.
6. Noise
interference.
1. During the test
process,
whether
there is
small 1. Enter "Service→Maintenance→Clean" interface and
Single HGB bubbles perform "Clean WBC Chamber" action; if the vacuole
11 repeatability is attached on overflows the cup mouth after blending, clean the inner
bad. the WBC wall of WBC chamber with soft, dustless cloth manually.
chamber 2. Check whether the luminous diode is normal.
wall
2. Luminous
diode is
broken.
1. WBC blending
1. Observe that during the measurement process, whether
failure.
bubble appears in two times of beating bubbles in WBC
Erythrocytes have
Normal sample chamber. If no bubble appears, refer to the according to
not been
12 WBC test the above correct error "No Bubble Comes Out in the
dissolved
value >20 Bubble Blending Process".
completely.
2. Check whether the pipeline has bubbles when adding
2. Lyse addition
Lyse and SV9 valve is working properly.
is not enough.
7.2.2.3 Other faults
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Chapter 8. System trouble shooting
8.1 Installation of operation system for upper machine

1. Prepare a USB startup disk and the mobile HDD in which the GHOST system applicable for the analyzer
is copied
2. Start the equipment
3. When LOGO is displayed on the screen, press the “DEL” key to enter the BOIS setup interface
4. Set the “SATA” mode as “AHCI” in “Advanced SATA Configuration”
5. Confirm that the following settings have been correctly set in “Chipset North Bridge”
Boot Display Device [LVDS]
Active LFP [LVDS]
Flat Panel Type [1366x768 LVDS]
6. Confirm that “Show Full Log” is set as “Disabled” in “BOOT”
7. Save and exit from BOIS setup
8. When LOGO is displayed after restart, press the “F11” key, select “General USB Flash Disk 1.0” in the
interface below and press the enter key
9. Enter the startup interface, select “1” to run “WIN8PEx86 Lite”

10. On completion of WIN8PE startup, start “ghost”, and use the function of “LocalDiskFrom Image”
11. Select the system image file (disk.gho), then select a local disk (with the volume of 500G), and start
to lead in after a series of confirmations. Then, after waiting for 20-30 minutes, the software will prompt
it has been finished. It is unnecessary to restart the system at once. Exit from ghost.
12. Start “DiskGenius”, in the menu of “Hard Disk reconstruct main boot record (MBR)”, and close the
software after success.
13. The WIN7operation system can start normally after restart.
14. After the system starts up, delete the automatically installed 360 Security and 360 Browser under “All
Programs 360 Safe” after startup; if such two kinds of software do not exist, skip the step.
15. Run “AOMEI OneKey Recovery”, and click “OneKey System Backup”
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16. Use default to select “Backup system to AOMEI OneKey Recovery Partition”, and click “Next”
17. Click “Start Backup”
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18. The backup takes about 15-20 minutes, and the backup is completed when the following interface is
displayed
8.2 Recover Application software.

When the application get error or some files or data lost, you have to recover the application from
backup.
The steps as follow:
1. Enter Win7 system interface, connect Mouse and Keyboard, press “Ctrl+Alt+Delete”,
Open the Task manager
Page 54 of 65
2. Click file, and create a new task, input “explore” then Click “OK”
Page 55 of 65
3. When machine start, it automatically start from AC 310 of D disk, the E disk is for backup,
once the application get error or files lost, you can copy the AC 310 of E disk .
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4. Copy the AC 310 folder from E disk backup, and cover the AC 310 folder in D disk.
8.3 Analyzer IP settings

Connect machine with Mouse and Keyboard, and then press Ctrl+Alt+Delete,
Open the task manager. Then create a new task to enter WIN7 system interface. Go to network
settings
If the outside not connect with net cable, and this
network will disconnect, and this network is which we
need to set IP for LIS connection. The IP we need to
set it in the same gateway as LIS server IP.
This network connection which always connect, it

connect to the main board. And the IP is fixed
192.168.100.5, it cannot be changed. When the
error “waiting connection”. It is this connection
have problem
Page 57 of 65
For the IP setting, please as below steps:
Enter connection properties, and double click

“internet protocol version 4(TCP/iPv4)
The picture is the IP settings for the connection

to main board
If the outside connection, when it connects with

LIS server, the IP set in the same gateway. For
example, LIS server IP is 192.168.1.100, we can
set the outside connection IP to: 192.168.1.
(1-254)
8.4 Main board IP capture tool introduction.

In the situation of main board IP changed by unknown reasons, the application will disconnect with main
board, and give the error “waiting connection”. At that time, we need to get the main board IP by IP
capture tool. Here we introduce the software “Wireshark-win32-1.12.4”, for this software, you can
download from internet directly, and install in our analyzer win7 system interface. After you installation,
then you open the software:
Page 58 of 65
Click Capture options icon, and select which network connection you want to
capture.
In our analyzer, capture the network which connect with main board, the name
may “Local area Connection”
After you press start, it will start capture IP, and several seconds, you can click stop icon
,and then you check the TCP protocol, and that is what we need.
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In our analyzer because that network connection only connect with main board, so in analyzer it will be
only one TCP, and the source IP is IPC address, and the Destination IP is main board IP address . Then set
the network IP same as destination IP of main board.
8.5 Application software update guide
1. Get the Update package from Wheisman Service. And unzip it to folder.
After you unzip, you will get this

folder
You get the update package is a Zip

or RAR file, you have to unzip it to
folder
2. Enter “Service --Software Update”, Select “Analyzer Software”, then Click Update.
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3. Select the unzip update package. Then Click OK.
4. After Click OK, it will pop-up below note, Click OK to continue.
5. Then it will pop-up below note, Click OK to restart application.
8.6 Some settings in windows system.

Ctrl+Alt+Delete, enter Win7 system interface, then press “Menu” Key in keyboard.
Page 61 of 65
Enter Control panel
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1. Power options settings
Set the “Choose when to turn off the display” and “Change when the computer sleeps”to never
turn off display and sleep as below:
Page 63 of 65
2. The windows firewall settings
3. Windows update settings
Page 64 of 65
Page 65 of 65

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AC 310 Hematology Analyzer

Wheisman Medical Technology Co., Ltd

Major warning signs used for the analyzer

Warning for laser

Warning for high voltage

Warning for puncture

MAJOR WARNING SIGNS USED FOR THE ANALYZER ............................................... 2

CHAPTER1. ANALYZER INSTALLATION ................................................................. 5

CHAPTER 2. HARDWARE SYSTEM..................................................................... 10

CHAPTER 3. SOFTWARE SYSTEM...................................................................... 16

CHAPTER 4. FLUID PATH SYSTEM ..................................................................... 19

4.3.2 CBC test procedure in pre-diluted mode ............................................................................................. 21

CHAPTER 5. MECHANICAL SYSTEM .................................................................. 28

CHAPTER 6. PARAMETER ADJUSTMENT SETTING............................................... 31

CHAPTER 7. MAINTENANCE AND CORRECT ERROR ............................................ 38

CHAPTER 8. SYSTEM TROUBLE SHOOTING ........................................................ 52

Chapter1. Analyzer installation

1.1 Analyzer unpacking

1.1.2 Handling method

1.2 Installation and environmental requirements

avoid the influence on the normal operation of the equipment.

1.3 Space requirement

1.4 Remove the auxiliary fixing structure for transportation

1.5 Connect the reagents and waste barrel

Picture of lower left interface on the back of the machine

Picture for connecting the machine side to reagent

Table 1-1 Reagent Interface Connection list

Interface Reagent Machine interface Interface color

4 Lyse W-31L Lyse Green

1.6 Power connection

1.8 Blank Test

Table 1-2 Blank Indicators

Parameter Blank value

Chapter 2. Hardware system

2.1 Introduction of boards:

2.1.1 Introduction of industrial control board:

2.1.3 Introduction of touch screen control board:

2.1.4 Introduction of DC-DC board:

2.1.5 Introduction of RFID Reader (for close system):

2.1.6 Introduction of main control board:

lower computers to execute;

2.1.7 Introduction of CBC pre-amplifying board:

2.1.8 LED display board:

2.1.9 Optocoupler detection board:

Definition of interface signal:

Position Name Definition

2.2 Location of each board in the analyzer

14----5V switch power supply

Chapter 3. Software system

3.1 Software architecture

systems of the middle computer and the lower computers.

The software architecture is shown in the figure below:

Upper Middle Slave Lower

Figure: Software System Architecture Chart

3.2 Software functions of upper computer

QC Report Calibration Setting WorkList Service Exit

L-J X-B X-AVG X-R Maintainece Self-Test Update

System Status Log Data Maintain

Manual Calibrator Fresh Blood History

Reference Print Dictionary Lab. Information Flag

Unit Option User Auto Maintain System Setting

Figure: Software Functions and Architecture Chart of Upper Computer

Classification of main functional modules:

Module name Functional description