Quality and Experience

Operating Manual
pfm Rotary 3006 EM
Please read this operating manual
carefully before using the instrument
for the first time and make certain
that you are familiar with the method
of operation as well as the instrument’s
operating elements to ensure that it
is used in the correct way.

www.pfmmedical.com
Note

Note
Dear customer,

Please read this instruction manual carefully before using the instrument for the first time and
make certain that you are familiar with the method of operation as well as the instrument‘s
operating elements to ensure that it is used in the correct way.

Serial number:

Above, please enter the serial number of your instrument, which can be found on the nameplate.

2 pfm Rotary 3006 EM

 Content

Part 1 – Safety instructions  5

1.1 Microtome safety devices 5
1.2 Operating the instrument 6
1.3 General precautions 6

Part 2 – Introduction 7
2.1 Views of the pfm Rotary 3006 EM 7
2.2 Details of the pfm Rotary 3006 EM 8
2.3 Technical data for the pfm Rotary 3006 EM 8
2.4 Device type, nameplate 9
2.5 Scope of delivery 9

Part 3 – Operating instructions 10

3.1 Unpacking and setting up 10
3.2 Initial operation 10
3.3 Switching on the instrument 10
3.4 Touchscreen control panel 11
3.5 Menu settings 11
3.6 Selecting Trimming and Coarse Feed modes 12
3.7 Function and adjustment of the Trimming and Coarse Feed modes 12
3.8 Setting the section thickness 12
3.9 Retraction of sample 12
3.10 End position 13
3.11 Sectioning speed 13
3.12 Setting the sectioning speed 13
3.13 Sectioning modes (manual/motor-driven) 13
3.14 Starting/stopping the sectioning process (motor-driven) 13
3.15 Sectioning the sample manually/motor-driven 14
3.16 Changing samples/interruption of work 15
3.17 Memo 15
3.18 Remaining feed indication 15
3.19 Section counter/addition 15
3.20 Mounting the object 16
3.21 Standard object clamp 16
3.22 Universal cassette clamp 16
3.23 Object orientation 16
3.24 Adjusting the orientable adapter 17
3.25 Setting the clearance angle 17
3.26 Connecting the foot switch (optional equiment) 17
3.27 Removinge/attaching the object clamp 18
3.28 Handwheel lock 18
3.29 Knife holder 18
3.30 Knife holder base 19
3.31 Knife holder mounting device 19
3.32 Disposable blade holder upper jaw low profile (narrow blade) 20
3.33 Section waste tray 20
3.34 Malfunctions and their correction 21

Rev.2017-04-07 3
Content

Part 4 – Working with the microtome 22

4.1 Preparing, embedding and sectioning the object 22
4.2 Information about how to avoid problems 22
4.3 Trouble-shooting 22

Part 5 – Microtome maintenance, cleaning and care 24

5.1 Maintenance 24
5.2 Cleaning and care 24
5.3 Returning the microtome for repair or maintenance 24
5.4 Instrument fuse 24
5.5 Warranty 25
5.6 Declaration of Conformity 25

Part 6 – Disposal of the microtome when put out of use 26

Part 7 – Accessories 27

4 pfm Rotary 3006 EM

 Safety instructions

Part 1 – Safety instructions

Please read this operating manual carefully before using the pfm Rotary 3006 EM. Make certain
that you are familiar with its control elements and the method of operation. Please observe the
following precaution measures during each operation of the instrument. Failure to observe these
precaution measures is contrary to the recognized standards of good practice and to the intended
use of the instrument.

If the above rule is violated, pfm medical ag shall assume no liability.

1.1 Microtome safety devices

The microtome is fitted with the following safety devices:

Finger protection on the knife holder

Before manipulating the knife, knife
1 holder or object, or changing the object,
always cover the cutting edge with the
finger guard!

1 Finger guard

Handwheel lock
When object and knife are being
mounted the handwheel lock should be
activated to prevent injury during the
mounting process. The M-STOP field
lights up when the handwheel is
1 locked.
M-STOP

1 Handwheel lock

Emergency Stop function

The E-STOP field lights up when the
Emergency Stop push button is pressed.
The emergency stop function can be
deactivated by turning the Emergency
1
Stop push button in the direction
indicated by the arrow. This must be
followed by a reference run (press
START to detect the reference position).

1 Emergency Stop push button E-STOP

Rev.2017-04-07 5
Safety instructions
1
1 Handle button 2 Handle
1.2 Operating the instrument
▸ Take care when handling knives or disposable blades.
The cutting edge is extremely sharp and may cause
severe injury.
▸ When setting up the object and the knife, the handwheel
should be locked in order to reduce any risk of injury
during this procedure. Please use the finger guard!
▸ I f possible, set up the object first before clamping the
knife/blade holder into the knife holder.
▸ A knife or blade holder not in use should always be kept
in the knife box.
▸N  ever try to catch a falling knife/blade holder.
1.3 General precautions
Please take special note of the following warnings:
Beware of explosive gases
The instrument may not be used if dangerous gas
concentrations could occur in the surroundings.
Risk of infection
When working with infectious materials, appropriate
safety measures must be implemented. The specimens used
may potentially be infectious. The general laboratory rules
regarding protection against infections must therefore be
observed.
Risk of radioactivity
When working with radioactive materials, the applicable
radiation protection regulations must be followed. Protective
clothing must be worn in accordance with the rules and
regulations that apply in the respective laboratory for
handling radioactively contaminated materials. For the
disposal of radioactive waste, the applicable regulations
will apply in each instance.
Avoiding malfunctions
To avoid malfunctions the instrument may not be used
close to radio transmitters, e.g. mobile phones. If the
instrument malfunctions please switch it off and contact
your dealer.
6 pfm Rotary 3006 EM
Removable handle
The handle can be removed when the
button on the end of the handle is
pressed. For safety reasons the handle
should always be removed for motor-
driven sectioning.
2
▸ Take care during sectioning and when removing sections.
▸ Always turn the handwheel clockwise.
▸ When sectioning take all necessary safety precautions (e.g.
wear protective goggles when sectioning brittle material).
▸ F or safety reasons always remove the handle for motor-
driven sectioning.
▸ Warning! Before starting the reference run always move
the knife holder to the front position. Otherwise damage
could be done to the knife holder, because, depending on
the position of the object holder, the latter moves
downward during the reference run.
Caution
Before switching the instrument on for the first time,
please check at the installation location that the supply
voltage and frequency match the information given on
the nameplate.
Protective earthing of the instrument
In order to avoid the risk of electrocution, the instrument
must be properly connected to an earth conductor. The
instrument is equipped with a mains power plug with
earth conductor. It is very important to check that the
mains power outlet used is equipped with an earth condtor,
and that it complies with the regulations of the International
Electrotechnical Commission (IEC).
Mains voltage precautions
Never remove housing components. The replacement of
components and alignment must be carried out only by
trained service technicians.
WARNING
C aution! Make sure that the electricity supply
is disconnected before removing any part of
the housing.
 Introduction

Part 2 – Introduction

2.1 Views of the pfm Rotary 3006 EM

1 Display
2 Control wheel Settings
3 Control wheel Coarse
Feed/Trimming
4 Control wheel Sectioning
Speed
1 8 5 Emergency Stop push
2 9 button
6 Clamping lever for
3 clearance angle adjustment
4
7 Clamping levers for knife
holder
5
8 Clamping lever for object
orientation
6 9 Handle button
10 10 Section waste tray

11 Finger guard
12 Pressure plate
13 Knife holder
14 Handwheel
15 Adjustment screws for
object orientation
16 Handwheel lock
14 17 Clamping lever for
15 pressure plate
18 Knife angle scale
11
12

13 16
17

18

Rev.2017-04-07 7
Introduction

1 Nameplate
2 Foot switch connection
3 Mains connection/
instrument fuse

2.2 Details of the pfm Rotary 3006 EM

▸ Ergonomic design ▸ Cutting window
▸B  acklash- and maintenance-free horizontal and vertical ▸ Memory function
cross-roller bearing system ▸ Section counter
▸ Smooth-running handwheel with removable handle ▸ Section thickness addition
▸ Handwheel locking system in each position ▸ Finger guard
▸ Quick change system for object clamps ▸ F oot switch
▸ Simple object orientation ▸ Section waste tray
▸ A ll electronic settings and functions are operated by a ▸ Anodised surface, easy to clean
touch screen

2.3 Technical data for the pfm Rotary 3006 EM

▸ Section thickness: 0.5 - 100 μm
▸ Setting range: 0.5 - 10 μm in 0.5 μm steps /
10 - 20 μm in 1 μm steps / 20 - 50 μm in 5 μm steps /
50 - 100 μm in 10 μm steps
▸ Trimming mode: 1 - 500 μm
▸ Coarse feed speed: 1.7 mm/sec.
▸ Sectioning modes: manual, continuous, single, step
▸ Sectioning speed max.: 300 mm/sec.
▸ Retraction: 0 - 100 μm in 5 μm steps
▸ Remaining feed indication 1 mm horizontal: visual and ▸ Weight: 38 kg
audible
▸ Horizontal range: 30 mm
▸V  ertical range: 70 mm
▸O  bject orientation: x- and y-axes universal 8°
z-axis up to 360°
▸M  aximum sample size:
▸ Universal cassette clamp, standard embedding cassettes
▸ Standard object clamp large, sample size 45 x 60 mm
▸ Universal cassette clamp large, super mega embedding
cassettes
▸ Power supply: 100/120/230/240 V AC, 50 - 60 Hz
▸ Dimensions (W/D/H): 450 x 530 x 275 mm

8 pfm Rotary 3006 EM

 Introduction

2.4 Device type, nameplate

The nameplate is attached to the back of the instrument.
The figure below is only an example.

2.5 Scope of delivery

pfm Rotary 3006 EM
REF: 0300610

Including
1 Disposable blade holder complete, low profile (narrow blade)
1 Section waste tray
1 Protective cover
1 Operating manual IFU2003006
Without object clamp
Without foot switch

Rev.2017-04-07 9
Operating instructions

Part 3 – Operating instructions

3.1 Unpacking and setting up

▸ Disassemble the packaging and remove the instrument.
▸ Do not carry or lift the instrument by the handwheel,
knife holder or control wheel.
▸ P lease observe the operating instructions contained in
the package.
▸ Position the instrument so that the handwheel is freely
and easily accessible.
▸ The microtome should be positioned on a stable, vibration-
free table.
▸ Position the instrument so that the power plug is always
accessible.
▸ I f other instruments in the vicinity of the microtome
generate vibrations, these can interfere with the
sectioning process.
▸ The room temperature should be between + 10 °C and + 40 °C.
▸ Relative air humidity should not exceed 80 %.

The operator is responsible for checking that the instrument is working properly and safely. The operator shall assume
responsibility for any use of special equipment and/or materials for operating the instrument.

3.2 Initial operation

▸ Before switching on the microtome ▸ The insert for the instrument fuse
for the first time, you must make sure (T2.0A) is situated next to the mains
that the supply voltage and frequency connection socket.
specified on the nameplate matches ▸ Switch on the mains power switch.
the mains voltage specifications at the ▸ Only use the mains power cable
installation location. supplied. It has a ferrite core.
▸ Connect the mains power cable with
the connection socket at the back of
1 2 3 the instrument.

1 Connection socket
2 Instrument fuse
3 Power switch

3.3 Switching on the ins trument

After switching on the instrument
an audible signal can be heard, and
“REFERENCING” is shown on the
display. Referencing is started by
pressing S TA R T . After this, the
display automatically goes back to the
control panel display.
WARNING
C aution! Before starting the
reference run always move
the knife holder to the front
position. Otherwise damage
could be done to the knife
holder, because, depending
on the position of the object
holder, the latter moves
downward during the
reference run.

10 pfm Rotary 3006 EM

 Operating instructions

3.4 Touchscreen control panel

1 Emergency stop
2 Modes
3 Section thickness
4 Trimming
16 1 15 5 Trimming automatically/manually
6 Retraction
2
7 Section counter
3 5 8 Remaining feed
9 Speed
4 10 Cutting Window on/off
6 11 Menu
7
12 Start
17
13 Delete
8 14 Memo
9 15 M-STOP
16 Control wheel Settings
10 14 17 Control wheel Coarse Feed/Trimming
18 Control wheel Sectioning Speed
11 13
18
12

3.5 Menu settings

Press the ME N U button to get
to a submenu with the following
subfunctions:
BUZZER
Press the BUZZE R button to switch
the buzzer on or off.
LANGUAGE
Press the L AN GUAGE button to
select between the different languages.
BRIGHTNESS
Press the BRIGH T N E SS button to
adjust the brightness of the display.
You can select one of three levels.
RESET/FACTORY DEFAULT SETTINGS
Press the RE SE T button to reset
the instrument to factory defaults.

Rev.2017-04-07 11
Operating instructions

3.6 Selecting Trimming and Coarse Feed modes

By pressing the Trimming/Coarse Feed control wheel
you can switch between trimming and coarse feed.

3.7 Function and adjustment of the Trimming and Coarse Feed modes
By pressing the Trimming/Coarse Feed control wheel you WA R NING
can switch between the Trimming and Coarse Feed modes. C aution! The advance via Trimming AUT is interrupted
Press the TRI M M I N G button to select the required value by only when the function is deactivated again using the
turning the Settings control wheel. Press the T R I MMIN G control wheel (turning it forwards).
button again or press the Settings control wheel to save the
set value. Manual trimming (Trimming MAN)
In manual trimming, infeed occurs only when the Trimming/
Trimming function Coarse Feed control wheel is turned forwards during sectioning.
The Trimming function can be used to select either manual or If the control wheel is released the infeed automatically
automatic sectioning of the specimen. The required function is reverts to the preselected section thickness.
activated by pressing the M AN or AU T button. When
the TRI M M I NG AU T function has been activated the Coarse feed functions
A UT button blinks. The coarse feed function is intended to quickly change the
distance from the sample to the knife or away from the
Adjusting the trimming thickness during the trimming knife. In the Coarse Feed mode the advance feed only works
It is also possible to change the preset trimming thickness when the Trimming/Coarse Feed control wheel is turned
during the trimming by turning the Settings control wheel. forward.

Automatic trimming (Trimming AUT)

In automatic trimming the function is activated by briefly
turning the Trimming/Coarse Feed control wheel forwards
and deactivated again by turning it forwards again. If the
function is deactivated the infeed automatically reverts to
the preselected section thickness.

3.8 Setting the section thickness

Press the T H I CKN E S S button to select the required Adjusting the section thickness during sectioning
value by turning the Settings control wheel. Press the It is also possible to change the preset section thickness
THICK NESS button again or press the Settings control during the sectioning by turning the Settings control
wheel to save the set value. wheel.

3.9 Retraction of sample

The retraction function is activated by pressing the
RETRA CT I ON button. Turn the Settings control wheel

to select the required value. Press the R E T R AC T I O N button

on the display again or press the Settings control wheel to
save the set value. When the value is 0, retraction is
deactivated. Retraction is activated during the upwards
motion in order to protect the knife/blade and object from
damage.

12 pfm Rotary 3006 EM

 Operating instructions

3.10 End position

If you stop the sectioning process, the instrument remains
in end position (Continuous mode “Up”, Stroke mode “Up”).
For stopping the sectioning operation, see 3.14.

3.11 Sectioning speed

The sectioning speed can be adjusted in the area of the
cutting window. To activate this function, press the
Cutting Window button. This enables you to reduce the
sectioning speed during sectioning.

3.12 Setting the sectioning speed

By turning the Sectioning Speed control wheel, the
sectioning speed can be continuously adjusted. Turning
it clockwise makes sectioning faster, and turning it
anticlockwise makes sectioning slower.

3.13 Sectioning modes (manual/motor-driven)

The instrument can be operated either manually or motor-
driven. You have one manual mode and three different
motor-driven modes at your disposal.
Selecting the mode
The desired operating mode can  be selected by directly
pressing the icons on the display.
Modes of operation
Manual
The sectioning movement is controlled manually by turning
the handwheel. The rotary motion is clockwise. The section is
made as the object is moving downwards.
In manual sectioning mode M AN the instrument can be
switched to CON continuous operation by using the start
button, the sectioning speed control wheel or the foot switch.
If the instrument is stopped, the operating mode automatically
changes back into manual sectioning mode M AN .
(This function is only available from version 4.4.3
onwards. For older versions before 4.4.3 please contact
pfm medical ag.)
Continuous (motor-driven)
Continuous sectioning operation until the instrument is
stopped. After that, the object stops in the selected end
position (Up).
Single (motor-driven)
Single stroke. After that, the object stops in the selected
end position (Up).
Step (motor-driven)
Continuous sectioning operation by permanently
pressing the ST ART button, the Sectioning Speed
control wheel, or by using permanently the foot switch.
On release, the object stops immediately.
WA R N I NG
C aut ion! In ma nua l sec t ion i ng mode MAN ,
usi ng t he star t but ton, t he sec t ion i ng speed
cont rol wheel or t he foot s w itch cha nges t he
i n st r u ment to cont i nuous operat ion CO N
a nd t he i n st r u ment star t s i m med iately.

3.14 Starting/stopping the sectioning operation (motor-driven)

The motor-driven sectioning operation can be started or
stopped by pressing the S T AR T button, by pressing the
Sectioning Speed control wheel or by using the foot switch.

Rev.2017-04-07 13
Operating instructions
3.15 Sectioning the sample manually/motor-driven
Manual operation
▸ Select the M A N mode.
▸ Release the handwheel lock.
▸ Coarsely advance the sample using the Trimming/Coarse
Feed control wheel in the Coarse Feed mode.
▸ Move the object towards the knife using the Coarse Feed
mode.
▸ Change to Trimming mode (AUT/MAN) by pressing the
Trimming/Coarse Feed control wheel and trim the sample
by turning the handwheel.
▸ If automatic trimming is deactivated (TRIMMING AUT)
or if the Trimming/Coarse Feed control wheel is released
(TRIMMING MAN) the infeed automatically reverts to the
preselected section thickness.
Continuous (motor-driven)
▸ Select the CON mode.
▸ Release the handwheel lock.
▸ Coarsely advance the sample using the Trimming/Coarse
Feed control wheel in the Coarse Feed mode.
▸ Move the object towards the knife using the Coarse Feed
mode.
▸ Select the appropriate sectioning speed using the Sectioning
Speed control wheel.
▸ Start the motor-driven sectioning operation.
▸ Change to Trimming mode (AUT/MAN) by pressing the
Trimming/Coarse Feed control wheel and trim the sample.
▸ If automatic trimming is deactivated (TRIMMING AUT)
or if the Trimming/Coarse Feed control wheel is released
(TRIMMING MAN) the infeed automatically reverts to the
preselected section thickness.
WARNING
C aut ion! The ha nd le shou ld be removed for motor- dr iven operat ion.
14
Single (motor-driven)
▸ Select the SI N G mode.
▸ Release the handwheel lock.
▸ Coarsely advance the sample using the Trimming/Coarse
Feed control wheel in the Coarse Feed mode.
▸ Move the object towards the knife using the Coarse Feed mode.
▸ Select the appropriate sectioning speed using the Sectioning
Speed control wheel.
▸ Start the motor-driven sectioning operation.
▸ The motor is set in motion for one stroke and afterwards
stops in the end position.
Step (motor-driven)
▸ Select the ST E P mode.
▸ Release the handwheel lock.
▸ Coarsely advance the sample using the Trimming/Coarse
Feed control wheel in the Coarse Feed mode.
▸ Move the object towards the knife using the Coarse Feed mode.
▸ Select the appropriate sectioning speed using the Sectioning
Speed control wheel.
▸ Start the motor-driven sectioning operation.
▸ The motor is set in motion for as long as the button
ST ART , the Sectioning Speed control wheel, or the foot
switch is pressed.
▸ On release, the motor stops immediately.
pfm Rotary 3006 EM
 Operating instructions

3.16 Changing samples/interruption of work

Changing samples
▸ When the instrument is stopped the motor stops in the
end position. (see Mode)
▸ Use the Trimming/Coarse Feed control wheel to change
to the Coarse Feed mode and make sure that the object is
moved far enough away from the knife.
▸ Lock the handwheel.
▸ Cover the knife blade with the finger guard.
▸ Change the sample.
Interruption of work
▸ When the instrument is stopped the motor stops in the
end position. (see Mode)
▸ Use the Trimming/Coarse Feed control wheel to change
to the Coarse Feed mode and make sure that the object is
moved far enough away from the knife.
▸ Lock the handwheel.
▸ Cover the knife blade with the finger guard.

3.17 Memo
By pressing the ME M O button (acoustic signal) the
sample’s present horizontal position is saved. By pressing
the M EM O button again the saved sample position is
restored.

3.18 Remaining feed indication

R-F EED 0 0 0 0 0
The remaining feed amount is indicated acoustically and
optically. The potential remaining feed amount in μm is
shown optically on the display. In the reference position
“Rear” the value 30000 is shown on the display. In the
end position “Front” the value 0 is shown. An acoustic
signal sounds between the positions 29000 and 30000 and
between 1000 and 0 to indicate that only 1000 μm (1 mm)
of feed remains.

1 WARNNING
Caution! A red marker on the
2 object support also indicates
that the front or rear end
position of the horizontal
stroke has been reached.

1 Rear end position

2 Front end position

3.19 Section counter/addition

A section counter is used to add up the sections performed.
After each turn of the handwheel the counter increases by
one. By pressing the D E L button the section counter is
set to zero.

Rev.2017-04-07 15
Operating instructions

3.20 Mounting the object

The object is mounted using different WARNING
clamping systems. An orientable adapter Caution! It is imperative
allows easy alignment of the object to that the handwheel be locked
1 the sectioning level. The orientable when working in the object
adapter can be screwed to the approprite orientation area.
clamp if needed.

1 Adapter orientable

3.21 Standard object clamp

The standard object clamp is used for
paraffin or plastic blocks of square or
rectangular shapes.

3.22 Universal cassette clamp

The universal cassette clamp, which WARNING
is operated via the clamping lever, is Caution! The contact surfaces
used for mounting standard embedding on the cheeks of the object
cassettes. The clamping lever must be clamps must be free of any
pulled upward to insert or withdraw residual paraffin. Otherwise
an embedding cassette. proper clamping cannot be
guaranteed.

3.23 Object orientation

In order to move the object to the
required position, the clamping lever
must first be loosened (red marker
1 point forward-facing). Movement of
2 the object along the X and Y axes of
about 8° in each direction is possible
using the coaxial adjusting screws.
The clamping lever is set to the clamp
position as soon as the required position
is reached. The set position is thus
fixed.

1 Clamping lever
2 Adjusting screws for object orientation

16 pfm Rotary 3006 EM

 Operating instructions

3.24 Adjusting the orientable adapter

To adjust the clamping of the orietable
adapter, first insert a screwdriver in
the hole in the adapter to fix it firmly.
Unscrew the nut with a 13 mm open
spanner. Then use an 8 mm open
spanner to screw the threaded brass
1
bolt inwards or out wards as required
2 and tighten the nut again with the 13
3 mm spanner. To test the clamping
insert the adapter in the guide bore
and tighten it with the clamping lever.
If fixation is not adequate the process
must be repeated and the brass bolt
adjusted in the required direction. It
must be possible to fix the clamping
lever at an angle of about 45° in both
directions.

1 Adapter hole
2 Nut
3 Threaded brass bolt

3.25 Setting the clearance angle

There are index markings on the right
hand side of the knife holder for setting
the clearance angle. Release the clamping
2 lever on the left hand side of the knife
holder and set the required clearance
angle. Then refix the knife holder in
the required position with the clamping
1 lever.

1 Knife angle scale

2 Clamping lever for setting
clearance angle

3.26 Connecting the foot switch (optional equipment)

The connecting socket for the foot switch is on the rear side WARNING
of the instrument. The foot switch can be used to operate the C aution! In manual sectioning mode MAN ,
motor-driven sectioning process (Start/Stop). In addition, it has using the start button, the sectioning speed
a similar function as the Emergency Stop push button. If the control wheel or the foot switch changes the
foot switch is pressed firmly, the emergency stop function is instrument to continuous operation CO N
activated. and the instrument starts immediately.

WARNING (This function is only available from version 4.4.3

C aution! Please do not turn or twist the plug onwards. For older versions before 4.4.3 please contact
in the connection socket! Please observe the pfm medical ag.)
guides on the foot switch plug.

Rev.2017-04-07 17
Operating instructions

3.27 Changing the object mounting device

To change the object clamp, loosen the
clamping lever and remove it from the
1 guide bore. Both adjusting screws must
2 be turned as far as the red markers. Then
the entire object mounting device, or
3
the clamp together with the orientable
adapter, can be removed upwards. The
new object clamp is fitted in reverse
order. Please observe the guide bore
position in the adapter to allow reinsertion
1 Clamping lever for object orientation of the clamping lever. After each
2 Adjusting screws for object orientation exchange, check to see if the clamping
3 Orientable adapter for object mounting lever is sufficiently clamped. If it is not
properly clamped, vibrations etc. can
occur while sectioning.

3.28 Handwheel lock

The handwheel can be locked within
360°. To lock it, the slide of the handwheel
lock is pushed all the way back. To
release the lock the slide must again
1 be pushed (in the opposite direction).
The M-STOP field lights up when the
2
handwheel is locked.

1 Handwheel
2 Handwheel lock

3.29 Knife holder

The knife holder consists of the knife
holder base and the disposable blade
1 holder upper jaw. The knife holder base
is used for mounting the upper jaw
2 of the knife holder and is fixed to the
base plate of the microtome above the
3 knife holder mounting device. In order
to facilitate cleaning of the disposable holder c a n b e adju s te d a s
blade holder, the upper jaw and the
pressure plate can be detached.
1 Finger guard
2 Disposable blade holder upper jaw
3 Knife holder base
WARNING
C aut ion! It i s i mp er at ive t h at
t he f i n ger g u a rd b e ac t iv ate d
a nd t he h a ndwhe el b e lo c ke d
du r i n g a l l work on t he k n i fe
holder. The p o sit ion of t he
c l a mpi n g le ver on t he k n i fe
ap propr i ate (3 6 0°).

18 pfm Rotary 3006 EM

 Operating instructions

3.30 Knife holder base

The knife holder base is used for
mounting the upper jaw of the knife
holder and is fixed to the base plate
of the microtome above the knife holder
mounting device. The knife holder
base can be released or clamped with
1 1 the knife holder clamping levers (on
the left and right).
2

1 Knife holder base 1 Knife holder base

2 Clamping levers for knife holder

3.31 Knife holder mounting device

The knife holder base is fixed to the
base plate of the microtome above the
knife holder mounting device. The knife
holder mounting device is operated
via the clamping levers on the left and
right hand side. The mounting device
1 and the precision guide must be free
of any residual paraffin and should be
2 cleaned regularly. The knife holder base
can be released or clamped using the
1 Knife holder mounting device clamping levers on the left and right.
2 Clamping levers for knife holder

Rev.2017-04-07 19
Operating instructions

3.32 Disposable blade holder upper jaw, low profile (narrow blade)
In order to fix the microtome blade in
the upper jaw of the disposable blade
holder with the pressure plate, the gap
1
between the pressure plate and the
mounting rail must first be opened.
2 3
To do this, move the clamping lever for
4 the pressure plate (right hand side) all
the way to its stop. Insert the blade.
During insertion of the blade, the
pressure plate should be pushed down
1 Pressure plate at the lower end to make best possible
2 Clamping lever for the pressure plate use of the gap. The clamping of the
3 Clamping lever for clearance angle blade is also made using this clamping
adjustment lever. The clearance angle is adjusted
4 Knife angle scale
via the scale on the knife holder base
and the index on the disposable blade
holder upper jaw. The clearance angle
adjustment is released or clamped
using the clamping lever on the left
hand side. Under usual circumstances
a clearance angle setting of 10° is
recommended for the pfm Rotary 3006 EM.
The upper jaw of the disposable blade
holder is available either as a low profile
blade holder for narrow blades or as a
high profile blade holder for wide blades.

1 1

1 1
Disposable blade holder upper jaw Disposable blade holder upper jaw
Low profile (narrow blade) High profile (wide blade)

3.33 Section waste tray

The section waste tray is anodised and

therefore easy to clean. It is slipped onto
the microtome on two guide rails at the
inside and positioned so that it is felt to
latch.

20 pfm Rotary 3006 EM


3.34 Malfunctions and their correction
Defect
No display after switching on
the instrument
Device does not start
No infeed during sectioning or
trimming of the sample
Foot switch does not work
Handwheel is hard to turn
The clamping eccentric cannot
be fully tightened
Rev.2017-04-07
Possible cause
Mains cable not correctly connected
Mains cable defective
Instrument fuse defective
Emergency stop button has been activated
Handwheel lock is activated
The object has reached the front position
Foot switch not connected correctly
Foot switch defective
Slide block
Clamping of the adapter is not correctly
adjusted
Operating instructions
Elimination
Check the mains cable connection
Check the mains fuse and replace if
necessary
Change the instrument fuse (see 6.4)
Deactivate emergency stop button
Release the handwheel lock
Move the object backwards using the coarse
feed function
Check connection to foot switch
Check foot switch and replace if necessary
Check setting of slide block
Adjust clamping of adapter (see 4.5)
21
Working with the microtome
Part 4 – Working with the microtome
4.1 Preparing, embedding and sectioning the object
The objects must be properly prepared and embedded in
suitable media before sectioning. Different forms and
rectangular specimens as well as the embedding cassette
are used for embedding. Depending on the embedding
form, the object is mounted, in the standard object clamp
or in the universal cassette clamp, and then trimmed.
Using the coarse feed (Coarse Feed/Trimming control wheel)
4.2 Information about how to avoid problems
Specimen preparation
When preparing the specimen, the correct fixation and
embedding medium should be selected as well as drainage
and infiltration times observed.
Specimen temperature
Heating of paraffin specimen through direct sunlight or
other heat sources should be avoided, otherwise the paraffin
will become soft so that it will no longer be possible to
section the specimen.
Clamping screws
Tighten all clamping screws on the knife holder and object
holder.
22
the object can be coarsely approached to the blade so that
there is a first contact between the object and the knife
blade. Sectioning can be accelerated using the trimming
function. When the required depth has been reached, turn
the handwheel to home position in order to change from
the trimming mode to the section thickness mode.
Clearance angle
The knife clearance angle should be selected depending on
the specimen.
Sectioning speed
The harder the material, the slower the sectioning speed.
Selection of the FEATHER® microtome blades
Information can be obtained directly from pfm medical ag
or from our responsible sales representative.
service@pfmmedical.com
T +49 (0)2236 9641-220
F +49 (0)2236 9641-51
www.pfmmedical.com
pfm Rotary 3006 EM

4.3 Trouble-shooting
Defect
Thick/Thin sections
Hand wheel does not run
smoothly
Vibration while sectioning
Sections compressed/ wrinkled
Ribbon sectioning is not
possible
Crooked sections
Sections rolls up
Sections too thick
Rev.2017-04-07
Possible cause
Microtome blade is insufficiently clamped
in the knife holder
Knife holder is insufficiently clamped
Object holder is insufficiently clamped
Incorrect knife holder clearance angle
Handwheel lock
Object holder is insufficiently clamped
Knife holder/pressure plate is insufficiently
clamped
Microtome blade in knife holder
Incorrect knife holder clearance angle
Paraffin block too warm
Incorrect knife holder clearance angle
Knife holder/pressure plate is insufficiently
clamped
Lateral edges of the block are not parallel
Blade edge and block are not parallel
Pressure plate is insufficiently clamped
Incorrect knife holder clearance angle
Microtome blade is still too sharp
Object holder is insufficiently clamped
Knife holder/pressure plate is insufficiently
clamped
Paraffin block too warm
Working with the microtome
Elimination
Check the clamping and clean
Check the clamping and clean
Check the clamping and clean
Check, adjust the clearance angle (10°)
Check operation/latching and clean
Check the clamping and clean
Check the clamping and clean
Check, adjust position, clean
Check, adjust the clearance angle (10°)
Cool down the block
Check, adjust the clearance angle (10°)
Check the clamping and clean
Move them into a parallel position
Align the block
Check the clamping and clean
Check, adjust the clearance angle (10°)
Longer trimming time required
Check the clamping and clean
Check the clamping and clean
Cool down the block
23
Maintenance, cleaning and care

Part 5 – Maintenance, cleaning and care

5.1 Maintenance
In order to guarantee the sectioning quality and operation of remains in faultless condition. Details can be obtained from
the rotary microtome, annual routine maintenance should your local sales representative or directly from pfm medical
be carried out by a qualified service technician. pfm medical ag.
ag can service your instrument to ensure that it always

5.2 Cleaning and care

It is recommended that the microtome be cleaned depending agents or solvents as these could damage any parts made
on the degree of dirt contamination and frequency of of plastic. The cross roller guides are protected against dirt
operation. To do this, mild household cleaning agents are and need not be cleaned.
suitable. Please do not use any aggressively acting cleaning

5.3 Returning the microtome for repair or maintenance

Generally, repair or maintenance work is carried out locally case, please use the original packaging only. If you do not
on site. If this should not be possible for any special reasons, have it any more, please contact our staff.
the device can be sent to pfm medical ag in Cologne. In this

5.4 Instrument fuse

The instrument fuse is situated on the rear of the instrument, the small protective cover with a small flat screwdriver and
and is integrated into the power switch. In order to replace remove the fuse holder. The fuse can then be replaced by a
the fuse, the instrument must be switched off at the mains new one.
power switch and the power plug must be unplugged. Open

1 2 3

1 Connection socket
2 Instrument fuse
3 Power switch

24 pfm Rotary 3006 EM

 Maintenance, cleaning and care

5.5 Maintenance, cleaning and care

We have checked and tested this device thoroughly.
However, in the event of any defect, apart from minor defects,
occurring in a device or spare part distributed by us up to
one year from delivery, we undertake to remedy the defect
to the extent specified below, without prejudice to any
statutory or other contractual claims, even where the defect
was not present on delivery to the customer, provided that
there has been no unauthorized interference with the
device by third parties. This does not include any assurance
of the equipment being free of wear and tear, or any guarantee
of the constitution or durability of the device.
The remedying of defects under this undertaking shall be
carried out, at our discretion, by the replacement or repair
of defective parts. Spare parts may be supplied for up to 5
years after the delivery of the device. Any warranty claims
on the part of the customer beyond the preceding are hereby
excluded, without prejudice to any statutory or other
contractual claims. This applies in particular to any claims
for compensation for damage, consequential damage or
costs relating to the defect. The transport of the device to
us and return transport during this period shall be at the
customer‘s risk.
To assert a claim under this undertaking, the device invol-
ved in the complaint must be sent to us, at the customer‘s
expense and risk, with a detailed description of the defect
complained of, specifying the purchase order number, delivery
note number and invoice number. Our address is as follows:
pfm medical ag, Wankelstrase 60, 50996 Köln, Germany.
Claims under this undertaking expire 6 months from assertion
of the claim, but not before the end of the claim period. This
undertaking and any claims, rights and obligations arising
therefrom are s ubject solely to the material law of the Fe-
deral Republic of Germany, to the exclusion of standards of
International Private Law and UN Law on the Sale of Goods.
Our general s upply and payment terms apply as
supplementary conditions.

5.6 Declaration of Conformity

The current Declaration of Conformity form can be found
on our website: http://www.pfmmedical.com.

Rev.2017-04-07 25
Disposal

Part 6 – Disposal of the microtome when put out of use

Our Environmental Contribution:

The WEEE directive 2002/96/EC (WEEE = Waste Electrical and Electronic Equipment; German: Elektro- und Elektronikge-
räte-Abfall) has been issued in Europe with the aim of reducing the increasing quantity of electronic scrap and electrical
and electronic devices that are no longer required in addition to disposing of them in an environmentally friendly manner.
In Germany, the directive was anchored in the 2005 Electrical and Electronic Equipment Act (ElektroG), that not only for-
med the basis for the implementation of the WEEE directive, but also for the RoHS (Limitation of Certain Dangerous Subs-
tances in Electrical and Electronic Equipment).

We, pfm medical ag, feel responsible for our products, from the initial idea to their disposal and see the situations as being
an opportunity for us to make our contribution to the conserving of resources. As the manufacturer, we assume responsi-
bility for the disposal of the decommissioned equipment on your behalf.

Please take the following action in order to return the electrical device:

1. Contact us using one of the following possibilities:

By telephone +49 2236 9641-0
By email: service@pfmmedical.com
By post: pfm medical ag, Abteilung Histotechnology, Wankelstr. 60, 50996 Köln, Germany

2. Prepare the device for transportation (please only use the original packaging) and commission a freight forwarder of
your choice with the return transport of the equipment to the following address:
pfm medical ag
Abteilung Histotechnology
Wankelstr. 60
50996 Köln, Germany

3. We bear the costs of the disposal as the manufacturer. Should nothing to the contrary be agreed to, the owner/user of
the device bears the transport and packaging costs.

26 pfm Rotary 3006 EM

 Accessories

Part 7 – Accessories
Universal cassette clamp
REF 010003
▸ Object clamp to be used with
orientable adapter or fixed
adapter
▸ Quick-release clamp system-
for all available cassettes
▸ Crossways and lengthways-
clamping possible
Universal cassette clamp,
orientable
REF 013019
▸ Universal cassette clamp,
orientable
▸ Quick-release clamp system
for all available cassettes
▸ Crossways and lengthways
clamping possible

Standard object clamp Standard object clamp,

REF 010004 orientable
▸ Object clamp to be used with REF 010044
orientable adapter or fixed ▸ Orientable version with
adapter adapter
▸ Maximum specimen size: ▸ Maximum specimen size:
40 x 45 mm 40 x 45 mm

Universal cassette clamp Universal cassette clamp

large
REF 013022
▸ Object clamp to be used with
orientable adapter or fixed
adapter
▸ Quick-release clamp system
for large embedding cassettes
▸ Crossways and lengthways
clamping possible
large, orientable
REF 013020
▸ Orientable version with
adapter
▸ Quick-release clamp system
for large embedding cassettes
▸ Crossways and lengthways
clamping possible

Standard object clamp Standard object clamp

large large, orientable
REF 013010 REF 013009
▸ Non-orientable version for ▸ Orientable version with
direct adaptation adapter
▸ For large specimens ▸ For large specimens
▸ Maximum specimen size ▸ Maximum specimen size
45 x 60 mm 45 x 60 mm

Adapter orientable Adapter fixed

REF 013016 REF 030055
▸ The orientable adapter is sui- ▸ Adapter for direct adaptation
table for pfm Slide and Rotary
microtomes.
▸ For object orientation

Rev.2017-04-07 27
Accessories

Foil clamp, Foil clamp,

non orientable orientable
REF 030056 REF 030057
▸ Spring loaded jaws secure the ▸ Spring loaded jaws secure the
specimen in position specimen in position
▸ To be used with orientable ▸ Orientable version with
adapter or fixed adapter adapter

Round specimen clamp, Insert for round

orientable specimen clamp, 6 mm
REF 030058 REF 030059
▸ For hard sectioning of round ▸ Diameter 6 mm
specimens (6, 15 and 25 mm
diameter)
▸ Orientable version with
adapter
▸ Without inserts

Insert for round Insert for round

specimen clamp, 15 mm specimen clamp, 25 mm
REF 030060 REF 030061
▸ Diameter 15 mm ▸ Diameter 25 mm

V-insert R-insert
REF 030062 REF 030063
▸ To be fitted in the standard ▸ To be fitted in the standard
object clamp object clamp
▸ To fix varible size of round ▸ For round specimens diameter
specimens 24-25 mm

Standard knife holder, Disposable blade holder,

upper jaw upper jaw, low profile
REF 030064 REF 030052
▸ For use with steel and ▸ For use with low profile
tungsten carbide knives up to disposable microtome blades
16 cm length. (narrow blades).
▸ With finger guard ▸ Removable pressure plate
▸ Easy to clean

28 pfm Rotary 3006 EM


Disposable blade holder,
upper jaw, high profile
REF 030053
▸ For use with high profile
disposable microtome blades
(wide blades).
▸ Removable pressure plate
▸ Easy to clean
Knife holder base
REF 030051
▸ Designed to hold the upper
jaw of the disposable blade
holder
▸ Fixed to the microtome base
plate using the knife holder
mounting device
▸ Removable
▸ Easy to clean
Foot switch
REF 030054
▸ To be fitted on the
pfm Rotary 3006 EM
Rev.2017-04-07
Accessories
Disposable blade holder
upper jaw, HP TC
REF 030065
▸ For use with FEATHER®
microtome blade HP TC
▸ Removable pressure plate
▸ To be fitted on the
pfm Rotary 3006 EM
▸ Easy to clean
Field magnifier set
with illumination
REF 030066
▸ Magnification 2.5 x
▸ For facilitate the knife/blade
alignment and approach for
sectioning
▸ Optimal view position
Section waste tray
REF 030050
▸ Material: anodised aluminium
▸ Easy to clean
29
Notes

For your notes

30 pfm Rotary 3006 EM

 Notes

Rev.2017-04-07 31
Contact
Should you have any questions our Customer
Solutions Team is happy to advise you.

service@pfmmedical.com
+49 (0)2236 9641-220
+49 (0)2236 9641-51

pfm medical ag Certified according to

Wankelstraße 60 DIN EN ISO 13485
50996 Köln
Germany

www.pfmmedical.com – Your information source for the products of pfm medical ag IFU2003006/Rev.2017-04-07