Republic of the Philippines

BATANGAS STATE UNIVERSITY

Gov. Pablo Borbon Campus 2
Alangilan, Batangas City

COLLEGE OF ENGINEERING, ARCHITECTURE AND FINE ARTS

Chemical and Food Engineering Department

ChE 555: BIOCHEMICAL ENGINEERING

ENGR. MELVIN P. JUSI

Instructor
BIOCHEMICAL ENGINEERING

BIOCHEMICAL ENGINEERING:

Biochemical Engineering is one of the major areas of biotechnology important to its

commercialization (Lee 1992).

Successful commercialization of biotechnology requires the development of a process

that is:

 TECHNOLOGICALLY VIABLE
 ECONOMICALLY EFFICIENT

Commercialization of BIOTECHNOLOGY involves scale up

from Laboratory Scale (small) Production Scale (large scale).
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M.P. Jusi, Ch.E.

BIOCHEMICAL ENGINEERING

BIOCHEMICAL PLANT: SMALL VS. LARGE

 Medium Preparation
 Sterilization
Upstream Process
 Inoculation
 Main Fermentation
 Product Separation
 Product Purification Downstream Process
 Packaging

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www.pubs.rsc.org
M.P. Jusi, Ch.E.
BIOCHEMICAL ENGINEERING

BIOCHEMICAL PLANT: SMALL VS. LARGE

MEDIUM PREPARATION

LAB SCALE LARGE SCALE

www.bioprocesscontrol.com

 medium components weighed,  medium components weighed,

place in flask, dissolved in water by put in bioreactor, dissolved in
stirring rod water by agitation system

 biochemical engineer calculates

the power requirements of agitator
motor

too low: inefficient mixing 4

www.bioprocessintl.com Too high: costly
M.P. Jusi, Ch.E.
BIOCHEMICAL ENGINEERING

BIOCHEMICAL PLANT: SMALL VS. LARGE

STERILIZATION

www.sunrisemachine.com
LAB SCALE LARGE SCALE

 autoclave (steam), 121 C, 10-15  steam injection into bioreactor

minutes jacket

Biochemical engineer calculates

steam requirements to achieve
sterilization

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www.keison.co.uk
M.P. Jusi, Ch.E.
BIOCHEMICAL ENGINEERING

BIOCHEMICAL PLANT: SMALL VS. LARGE

INOCULATION

www.blbio.com
LAB SCALE LARGE SCALE

 Wire loop  progressive inoculation using

 Sterile Pipettes seed fermentors

www.news.wisc.com  biochemical engineering “rule of

thumb” for inoculation size: 3% to
10% of reactor volume

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www.medsupplypartners.com
M.P. Jusi, Ch.E.
BIOCHEMICAL ENGINEERING

BIOCHEMICAL PLANT: SMALL VS. LARGE

FERMENTATION

LAB SCALE LARGE SCALE www.biobasenwe.org

 flasks, petri plates, small  large scale bioreactors

fermenters
 biochemical engineers
determine power requirements for
agitation equipment, cooling
water requirements, aeration rates
(for aerobic cultivation)
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www.wikipedia.org M.P. Jusi, Ch.E.

BIOCHEMICAL ENGINEERING

BIOCHEMICAL PLANT: SMALL VS. LARGE

DOWNSTREAM PROCESSING

LAB SCALE LARGE SCALE www.waterworld.com

 Lab scale centrifuges, filters,  large equipment for product

chromatography equipment, etc. separation and purification

Cell separation centrifuge

Chromatography columns
Filters
www.westfalia-separator.com
www.lab-kits.com 8

M.P. Jusi, Ch.E.