KIMIA PEMISAHAN KI310

SOAL KROMATOGRAFI

Tanggal :
Senin, 17 Oktober 2022

Dosen Pengampu:
Dr. Soja Siti Fatimah, S.Si., M.Si.

Kelompok 8:
Andini Salsabila 2102575
Mochamad Fajar Islam AS 2100190
Nadya Anjani 2102477

DEPARTEMEN PENDIDIKAN KIMIA

FAKULTAS PENDIDIKAN MATEMATIKA DAN
ILMU PENGETAHUAN ALAM
UNIVERSITAS PENDIDIKAN INDONESIA
BANDUNG
2022
32. From distribution studies, species M and N are known to have
water/hexane distribution constans of 5,99 and 6,16 (K =[X] H2O/[X]hex),
where X = M or N. the two species are to be separated by elution with
hexane in a coloumn packed with silica gel containing adsorbed water.
The ratio Vs/Vm for packing is 0,425
a. Calculate the retention factor for each solute.
Faktor retensi (k) untuk spesi M
𝑉𝑠
𝐾𝑚 = 𝐾𝐴
𝑉𝑚
𝐾 = 5,99 𝑥 0,425 = 2,54
Faktor retensi (k) untuk spesi N
𝑉𝑠
𝐾𝑁 = 𝐾𝐴
𝑉𝑚
𝐾 = 6,16 𝑥 0,425 = 2,61

b. Calculate the selectivity factor

𝐾𝑁
∝=
𝐾𝑀
2,61
∝=
2,54
∝= 1,027
c. How many plate are needed to provide a resoliton of 1,5
𝛼 2 1 + 𝐾𝐵
𝑁 = 16(𝑅𝑆 )2 ( ) ( )
𝛼−1 𝐾𝐵
2
1,027 2 1 + 2,61 2
𝑁 = 16(1,5) ( ) ( )
1,027 − 1 2,61
𝑁 = 99643,56

d. How long a coloumn is needed if the plate height of the packing is

1,5 x 10-3 cm
𝐿 = 𝑁𝐻
𝐿 = 99643,56 𝑥 1,5 𝑥 10−3
𝐿 = 1,49 𝑥 102 𝑐𝑚
e. If the flow rate is 6,75 cm/min, how long will it tahe to elute the
two species?
16(𝑅𝑆 )2 𝐻 𝛼 2 1 + 𝐾𝐵 2
(𝑇𝑅 )𝐵 = ( ) ( )
𝑢 𝛼−1 𝐾𝐵
16(1,5)2 (1,5 𝑥 10−3 ) 1,027 2 1 + 2,61 2
(𝑇𝑅 )𝐵 = ( ) ( )
6,75 1,027 − 1 2,61
(𝑇𝑅 )𝐵 = 22,14 𝑚𝑒𝑛𝑖𝑡
33. repeat the calculation is problem 32 assuming assuming KM = 5,81
and KN = 6,20
a. Calculate the retention factor for each solute.
KM = 5,81
KN = 6,20
b. Calculate the selectivity factor
𝐾𝑁
∝=
𝐾𝑀
6,20
∝=
5,81
∝= 1,06

c. How many plate are needed to provide a resoliton of 1,5

2
𝛼 2 1 + 𝐾𝐵
𝑁 = 16(𝑅𝑆 ) ( ) ( )
𝛼−1 𝐾𝐵
2
1,06 2 1 + 6,20 2
𝑁 = 16(1,5) ( ) ( )
1,06 − 1 6,20
𝑁 = 15152,81

d. How long a coloumn is needed if the plate height of the packing is

1,5 x 10-3 cm
𝐿 = 𝑁𝐻
𝐿 = 15152,81 𝑥 1,5 𝑥 10−3
𝐿 = 102 𝑐𝑚

e. If the flow rate is 6,75 cm/min, how long will it tahe to elute the
two species?
16(𝑅𝑆 )2 𝐻 𝛼 2 1 + 𝐾𝐵 2
(𝑇𝑅 )𝐵 = ( ) ( )
𝑢 𝛼−1 𝐾𝐵
16(1,5)2 (1,5 𝑥 10−3 ) 1,06 2 1 + 6,20 2
(𝑇𝑅 )𝐵 = ( ) ( )
6,75 1,06 − 1 6,20
(𝑇𝑅 )𝐵 = 3,36 𝑚𝑒𝑛𝑖𝑡