Dermaclarine™

A natural and gentle skin clarifier

 Background
Dermaclarine™ is part of ABT's patented skin care actives extracted from
Norwegian fresh water after salmons have hatched. Hatching water is a
complex protein solution that has proven to have a wide range of benefits
for human skin. Dermaclarine™ is a result of research done by the
University of Bergen whose scientists were intrigued by the fact that salmon
hatchery workers' hands became smooth and soft after sorting salmon eggs
despite the prolonged exposure to cold water. They discovered unique
enzymes and proteins released in the water where salmon eggs hatch.
Dermaclarine™ is a concentrated fraction of this potent cocktail.

Key features
Dermaclarine™ is a patented ingredient derived from the hatching water of salmon roe or “red
caviar” as it is otherwise known. Dermaclarine™ is:
 Novel and unique.
 A biomimetic enzymatic alternative to AHAs, able to render comparable exfoliating results
without any irritation.
 Proven to significantly reduce skin imperfections in vivo: effectively clears pores, visibly
diminishes their appearance, and minimizes the number of comedones, pustules, and pimples,
while keeping the skin moisturized.
 100% natural and sustainable.
 Highly stable, unlike other enzymes, and easy to formulate.
Dermaclarine™ is a gentle ingredient for a clear and refined complexion.

How it works
Two types of proteins from salmon hatching water are
exfoliating corneocyte
concentrated in Dermaclarine™:
corneodesmosome
1. Proteases that help the larva getting out of its eggshell degraded by proteases
by digesting this tough, fibrous protein structure but
without harming the delicate baby fish, gently exfoliate
corneodesmosome
dead skin cells. Like the enzymes naturally secreted by
human skin, these proteases degrade specific cell
binding sites in the stratum corneum, corneodesmo-
somes. Dead skin cells are gently detached while the
living skin cells beneath remain untouched.
Hence, the smooth skin of the hatchery workers!
1
2. Egg shell proteins penetrate and transport moisture stratum corneum 2
into the stratum corneum that hydrates the skin.
Acne occurs in part when there is a buildup of dead skin cells in the pores (hyper-keratinization
of the sebaceous canal). Dermaclarine™ helps normalize the shedding of the skin cells inside
the pores and prevent the clogging that, along with oil and bacteria, can produce skin lesions.
non-inflamed lesions inflamed lesions

whitehead blackhead pustule pimple

sebaceous duct
duct clogged by
gland dead cells,
sebum
starts to
accumulate
Efficacy in vivo
Exfoliation effect of Dermaclarine™ vs. glycolic acid (pilot study) Scaly skin treated with
Dermaclarine™ at 3%
The assessment of exfoliation on 3 subjects using D-Squames® revealed a
higher reduction in scaliness on average for Dermaclarine at 3% compared
to glycolic acid at 3% after treatment regimens of 5 min, 10 min or 15 min.
Exfoliation (D-Squames® scoring) difference to baseline
0,6

0,5 t0
0,4
3% Dermaclarine™
0,3
3% glycolic acid
0,2
0,1
0,0
0 min 5 min 10 min 5 min
15 min

Effect of Dermaclarine™ on skin imperfections

Twice daily application on split face of a semi-fluid gel containing 1% Dermaclarine™ against
untreated for 28 days
Panel of 21 women with moderate facial acne, measurement: number of lesions
Panel of 23 women with visible facial pores, measurement: average pore diameter

Average number of lesions

10
8.4
8 6.9 7

6 -61%**
3.2 3.5 2.7
4 2.8
2 1.6
-43%* inflamed
0 non-inflamed
t0 t1=28 days t0 t1=28 days
untreated
1% Dermaclarine™

Variation of average pore diameter relative to t0

-35%
-32%
-30%

-25% ***
-19.9 %
-20%
mean t0
-15%
max.
-10%
-5%
untreated 1% Dermaclarine™
0%
5% 3.5 %

A reduction in pore diameter was measured in 87% of the panellists. 28 days

(*p<0.05% **p<0.01% ***p<0.001%)
Specifications
 INCI name (proposed): Phosphate Buffered Saline (and) Hydrolyzed Egg Protein (and) Protease
 Physical aspect: clear, colorless and odorless solution
 Preservative free
 pH range: 5.5 – 9, with maximum activity around pH 7.5
 Solubility: water soluble
 Stability: stable in emulsions for more than 3 years under normal conditions
 Toxicology: not toxic for humans (non-irritating, non-phototoxic, non-sensitizing, non-mutagenic) or
the environment
 Safety: GMO-free, BSE-free, CMR-free, free from heavy metals
 Recommended use level: 1%

Dermaclarine™ can be used in most topical skincare products, even for the most delicate skins,
but it is especially suitable for:
 Treatments for combination and/or oily skin, blemished skin
 Pore minimizing formulas
 Skin perfecting treatments
 BB creams

Sustainability
Designed by nature, Dermaclarine™ is obtained from a sustainable source and is 100%
biodegradable. It is extracted from the water after the natural hatching process is completed,
without harming animals in any way. Dermaclarine™ is collected at a hatchery by a pristine
fjord. Salmon eggs hatch in fresh water sourced from a secluded lake famed for the quality of its
water. The production process is eco-friendly: low energy consumption and no environmental
impact.

P.O. Box 107

N-1325 Lysaker, Norway
Fax: +47 67 11 25 41
www.aquabiotechnology.com
info@aquabiotech.no

Disclaimer
Utilization of this document or parts thereof as well as product names for commercial or industrial applications is
subject to explicit written approval by Aqua Bio Technology ASA. This information is based on our own experience
to date and we believe it to be reliable. It is intended only as a guide to use at your discretion and risk. We cannot
guarantee favourable results and assume no liability in connection with its use, or the use of the methods or
products described. None of this information is to be taken as a license to operate under, or a recommendation to
infringe patents.
Version: 09/2012