Carlos, Jackielou B.

11/10/2022

STEM 11-A

Test for Sugar Use/Function Observable Results Illustration

A. General Tests g h

1. Molish Test -is a chemical test which is used -the formation of a purple or a
to detect for the presence of purplish-red ring at the point of
carbohydrate and glycoprotein contact between the H2SO4 and
in a given analyte the analyte + Molisch’s reagent
mixture confirms the presence

g of carbohydrates in the analyte

h

2. Anthone Test -is a group test for -the presence of a blue-green

carbohydrates that provides a complex indicates the presence
rapid and convenient method of carbohydrates in the given
for quantification of solution
carbohydrates in various
samples like blood serum, milk,
and its variation, etc. that are
either free or bound to any
lipids or proteins

-is used for the detection and

quantification of carbohydrates
in various samples like blood
serum, milk, and its variation,
etc.

B. Test for the

presence of starch g G
1. Iodine Test -can be used for the detection -the appearance of blue-black
of starch in a given sample that color indicates the presence of
can help to distinguish starch the starch in the sample
from monosaccharides,
disaccharides, and other
polysaccharides

C. Qualitative Tests

1. Benedict's Test - is used to detect the presence -if the color upon boiling is
of glucose in blood and urine changed into green, then there
would be 0.1 to 0.5 percent
-used as a simple test for sugar in solution
reducing sugars. A reducing
sugar is a carbohydrate -if it changes color to yellow,
possessing either a free then 0.5 to 1 percent sugar is
aldehyde or free ketone present
functional group as part of its
molecular structure -if it changes to orange, then it
means that 1 to 1.5 percent
sugar is present

-if color changes to red,then 1.5

to 2.0 percent sugar is present

-and if color changes to brick

red, it means that more than 2
percent sugar is present in
solution
 2. Barfoed's Test -this test distinguishes a -if precipitate appear in 7
reducing monosaccharide from minutes, it means
a reducing disaccharide monosaccharde is present in
the original solution. But if
precipitate appear after seven
minutes, it means that
disaccharide is present in the
original solution

3. Seliwanoff's Test -is used to distinguish adoses -when showing cherry red color
(sugars that have an aldehyde solution, it means sugar that be
group) from ketoses (sugars tested is / contains ketose sugars
that have an aldehyde group)

-used in the method for the

colorimetric determination of
fructose in fermentation media

4. Bial's Orcino Test -performed to detect the -the visible positive results for
presence of pentoses and Bial’s Orcinol is a blue or green
pentosans (derivatives of solution and this should be
pentoses) exhibited in Xylose because it is
a pentose
-This test can additionally be
used for the quantification of -all other colors except blue and
RNA in a sample. green indicates a negative result

References:

1. Vix. (2022, June 16). Iodine test - definition and examples - biology online dictionary. Biology Articles, Tutorials &
Dictionary Online. Retrieved November 10, 2022, from https://www.biologyonline.com/dictionary/iodine-test

2. Biochemistry Den. (2022, April 27). 7 color reactions of carbohydrates (practical protocol). Biochemistry Den. Retrieved
November 10, 2022, from https://biochemden.com/color-reactions-of-carbohydrates/

3. Seliwanoff's test for ketose sugars. edubio.info. (1970, January 1). Retrieved November 10, 2022, from
http://en.edubio.info/2017/02/seliwanoffs-test-for-ketose-sugars.html?m=1

4. Orcinol Testu. Biology Articles, Tutorials & Dictionary Online. (2022, July 24). Retrieved November 10, 2022, from
https://www.biologyonline.com/dictionary/orcinol-test

5. Scribd. (n.d.). Bial's orcinol test. Scribd. Retrieved November 10, 2022, from
https://www.scribd.com/document/430192016/Bial-s-Orcinol-Test

6. Aryal, S. et al.(2022, August 10). Benedict's test- principle, preparation, procedure and result interpretationh. Microbiology
Info.com. Retrieved November 10, 2022, from https://microbiologyinfo.com/benedicts-test-principle-composition-
preparation-procedure-and-result-interpretation/

7. Sapkota, A. & Fynn, D. M. (2022, September 5). Anthrone test- definition, principle, procedure, result, uses. Microbe Notes.
Retrieved November 10, 2022, from https://microbenotes.com/anthrone-test/#result-and-interpretation-of-
anthrone-test

8. Arshad, H. et al.(2016, February 4). Barfoed's test for Monosaccharides. All Medical Stuff. Retrieved November 10, 2022,
from https://allmedicalstuff.com/barfoeds-test/