Metal Abs

1.00E-04 0.122 0.7

2.00E-04 0.251
3.00E-04 0.376 0.6 f(x) = 1251 x − 0.00129999999999997
4.00E-04 0.496 R² = 0.999860083566527
5.00E-04 0.625 0.5

0.4

0.3

0.2

0.1

0
05 04 04 04 04 04 04 04 04 04 04
0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E-
0 0 5 0 5 0 5 0 5 0 5
5. 1. 1. 2. 2. 3. 3. 4. 4. 5. 5.

Ligand Abs
1.2
6.00E-04 0.752
7.00E-04 0.873
1
8.00E-04 0.937 f(x) = 589 x + 0.434
9.00E-04 0.962 R² = 0.910200237178209
0.8
1.00E-03 1.002

0.6

0.4

0.2

0
04 04 04 04 04 04 04 04 04 03 03
0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 5E-
5 0 5 0 5 0 5 0 5 0 0
5. 6. 6. 7. 7. 8. 8. 9. 9. 1. 1.
 0 4 04 04 04
E- E- E- E-
.00 50 00 50
4 4. 5. 5.

- 04 0 4 03 03
0E E- E- E-
.0 50 00 05
9. 1. 1.
Metal Abs
0.08
1.00E-05 0.012
2.00E-05 0.029 0.07
3.00E-05 0.042 f(x) = 1400 x − 0.000599999999999996
0.06 R² = 0.997353958884592
4.00E-05 0.055
5.00E-05 0.069 0.05

0.04

0.03

0.02

0.01

0
06 05 05 05 05 05 05 05 05
0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E-
0 0 5 0 5 0 5 0 5
5. 1. 1. 2. 2. 3. 3. 4. 4.

Ligand Abs
1.00E-05 0.040 0.250
2.00E-05 0.085
3.00E-05 0.125 0.200
f(x) = 4090 x + 0.00089999999999997
4.00E-05 0.162 R² = 0.998978811838616
5.00E-05 0.206
0.150

0.100

0.050

0.000
06 05 05 05 05 05 05 05 05
0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E- 0E-
0 0 5 0 5 0 5 0 5
5. 1. 1. 2. 2. 3. 3. 4. 4.
9999999999996
92

05 05 05 05 05 05
E- E- E- E- E- E-
. 00 . 50 . 00 .50 .00 . 50
3 3 4 4 5 5

9999999999997
616

05 05 05 05 05 05
E- E- E- E- E- E-
.00 . 50 .00 . 50 . 00 .50
3 3 4 4 5 5
 0.9

x y 0.8
VL VM VL/(VL + VM) Abs f(x) = 1.28892857142857 x + 0.000
0.7 R² = 0.999938659632357
0 1 0 0.001
0.1 0.9 0.1 0.126 0.6
0.2 0.8 0.2 0.26 0.5
0.3 0.7 0.3 0.389
0.4 0.6 0.4 0.515 0.4
0.5 0.5 0.5 0.642 0.3
0.6 0.4 0.6 0.775
0.7 0.3 0.7 0.771 0.2
0.8 0.2 0.8 0.513 0.1
0.9 0.1 0.9 0.253
1 0 1 0
0 0 0.2 0.4

Point of Interscetion= ~0.68

Ratio:
m b E
1 1.2889 0.0002 2502.7184466
2 2.573 2.5713 4996.1165049
Mean 3749.4174757
f(x) = 1.28892857142857 x + 0.000178571428571472
f(x) = − 2.573 x + 2.5713
R² = 0.999938659632357 R² = 0.999968884689833

0.2 0.4 0.6 0.8 1 1.2

 Vol Fluorescence
0.00 6.12 25
4.00 11.16
8.00 15.68
12.00 20.64 20 f(x) = 1.202 x + 6.188
R² = 0.999587657395877

15

10

0
0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00
10.00 12.00 14.00
 Intensity F0/F
Conc.
298 310 298 K 310 K
0 1158.7 1077 1.0000 1.000
0.000003 950.7 948.2 1.2188 1.136
0.000006 863.1 855.1 1.3425 1.260
0.000009 740.4 770.9 1.5650 1.397
0.000012 679.7 689.5 1.7047 1.562 1.8000
1.6000 f(x) = 58520.8023270791 x + 1.01506720
R² = 0.992659897260259
1.4000 f(x) = 46174.4980285561 x + 0.99383460
R² = 0.997246351540725
1.2000
1.0000

I ntensity
0.8000
298 K 310 K
0.6000
Slope 58521 46174
Ksv 58521 46174 0.4000

To 1.0x10^-8 1.0x10^-8 0.2000

0.0000
0 0.000002 0.000004 0.000006 0.000008
T(K) Ksv R2 Kq Concentration
298 58521 0.9927 5.8521E+12
310 46174 0.0072 4.6174E+12
520.8023270791 x + 1.01506720593025
2659897260259
174.4980285561 x + 0.993834601045902
7246351540725

002 0.000004 0.000006 0.000008 0.00001 0.000012 0.000014

Concentration