NORTHERN TECHNICAL UNIVERSITY

TECHNICAL COLLEGE MOSUL

MEDICAL INSTRUMENTATION ENGINEERING

Report
CLINICAL CHEMISTRY
EXP-1:OXIDATION-REDUCTION TITRATION

Supervisor:Dr.Asma Bakr

Preparation
Fatima Ahmed
Mawada Saud
Mohamed Nouri
Mustafa Manhal
Maytham Muthana
Mohamed abd AlQader

week1 Second Stage 2022

chemistry

Aim of Experiment
Determination of oxalate by titration with potassium permanganate

Principle
Titration of KMnO4 vs. H2C2O4 is oxidation-reduction reaction in

)which the electrons are transferred from the reducing agent (oxalic acid
to the oxidizing agent, (Potassium permanganate) resulting in a net
change in the oxidation state numbers of one or more elements in the
.reacting substances
Oxidation: is the process of electron loss by reducing agent.

Reduction: is the process of electron-gain by the oxidizing agent

Oxidizing agent: is one that gains electron and goes to a more, negative

oxidation state.
Reducing agent: is one that loses electrons and goes to a more positive
oxidation state.
Oxidation and reduction occur simultaneously and the number of

electrons lost in the oxidation must be equal to the number of electrons

.gained in the reduction
The titration of KMnO4 vs oxalic acid is a used example of the

oxidation-reduction reaction in which KMnO4 solution is considered a

strong standard oxidizing agent and H2C2O4 a strong reducing agent
acting as the solution of unknown concentration to be calculated in terms
.)of normality units (gm.eg/l

Reaction

Reagents
* 0.1 N KMnO4:
Dissolve 3.16 g of KMnO4 / L of water. To prepare 0.01 N KMnO4,
.)dilute as necessary (1:10
* 0.01 N H2C2O 4:
dissolve 6.3 g of H2C2O4 / L of water. To prepare 0.01 N H2C2O4, dilute
.)as necessary (1:10
* 2N H2SO4:
56.4 ml of conc. H2SO4 / L of water.

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chemistry

Procedure
1. Put 1.0 ml of oxalic acid in an Erlenmeyer flask.

2. Add 1.0 ml of 2N H2SO4.

3. Add 0.5 ml of distilled water.

4. Heat the solution to about 80 C

5. Titrate (with the test tube still in the water bath) with 0.01 N of

,KMnO4 solution, which is added from a graduated 2 ml pipette

until the appearance of a pink color indicating the end pointof the
.reaction
6. Record the volume of KMnO4 used. Repeat the whole process at
least in duplicate. The difference between result should not exceed
.ml 0.2

Calculations
M.W. of KMnO4 = 158.05
N contains 158.05 / 5 = 31.61 g/L 1
M.W. of H2C2O4 = 90
N contains 90 / 2 = 45 g/L 1

Discussion
1-Why we add D.W in the solution?
-To make the solution up to the marked point with and make sure the
.oxalic acid is fully dissolved

2-Why we add H2SO4 to the solution?

- is because KMnO4 act as oxidising agent only in acidic medium so , in

.order to provide acidic medium dilute Sulphuric acid is added

3-Why we should heat the solution to 80c and must not get
further?
-This is because increasing the temperature increases the Kinetic energy,
and hence more molecules would have energy greater than the activation
energy resulting in increased rate and completion of the reaction, mustn't
.get further because Oxalic acid begins to decompose

4-What the reason of appearing the brown color?

-The reason is that the solution is not heated, or the use of a dirty flask.

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