Scribd Logo
Upload

Welcome to Scribd!

  • Mutaciones ..

    Uploaded by

    Mayra Jimena
    12 views11 pages
    This document presents the DNA sequences of the KRAS gene from a healthy cell and tumor cell. There are 6 regions of the KRAS gene sequenced from both cells. By comparing the sequences, 4 differences are identified between the healthy and tumor cell DNA. These differences result in amino acid changes from glycine to valine (position 10), glycine to aspartic acid (position 12), glutamine to arginine (position 61), and alanine to proline (position 10). These amino acid changes are potential cancer-causing mutations.

    Original Description:

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    This document presents the DNA sequences of the KRAS gene from a healthy cell and tumor cell. There are 6 regions of the KRAS gene sequenced from both cells. By comparing the sequences, 4 differences are identified between the healthy and tumor cell DNA. These differences result in amino acid changes from glycine to valine (position 10), glycine to aspartic acid (position 12), glutamine to arginine (position 61), and alanine to proline (position 10). These amino acid changes are potential cancer-causing mutations.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    12 views11 pages

    Mutaciones ..

    Uploaded by

    Mayra Jimena
    This document presents the DNA sequences of the KRAS gene from a healthy cell and tumor cell. There are 6 regions of the KRAS gene sequenced from both cells. By comparing the sequences, 4 differences are identified between the healthy and tumor cell DNA. These differences result in amino acid changes from glycine to valine (position 10), glycine to aspartic acid (position 12), glutamine to arginine (position 61), and alanine to proline (position 10). These amino acid changes are potential cancer-causing mutations.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 11

    NOMBRE: Apaza Charca Mayra

    Can you spot a cancer mutation?


    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    30 40 50 270 280 290

    GTAGTTGGAGCTGGTGGCGTAGGCAAGAGT AAATCATTTGAAGATATTCACCATTATAG

    Tumour cell DNA Tumour cell DNA

    30 40 50 270 280 290

    GTAGTTGGAGCTGTTGGCGTAGGCAAGAGT AAATCATTTGAAGATATTCACCATTATAG

    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    10 GGT GTT Glicina Valina No presenta mutaciones , Secuencia conservada
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    30 40 50 210 220 230

    GTAGTTGGAGCTGGTGGCGTAGGCAAGAGT AGGGACCAGTACATGAGGACTGGGGAGGGC

    Tumour cell DNA Tumour cell DNA

    30 40 50 210 220 230

    G
    GTAGTTGGAGCTGGTG G
    A
    CGTAGGCAAGAGT AGGGACCAGTACATGAGGACTGGGGAGGGC
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    12 GGC GAC Gly Asp No presenta mutaciones Secuencias conservadas
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    350 360 370 80 90 100


    AATAAATGTGATTTGCCTTCTAGAACAGTA CATTTTGTGGACGAATATGATCCAACAATA

    Tumour cell DNA Tumour cell DNA

    350 360 370 80 90 100


    AATAAATGTGATTTGCCTTCTAGAACAGTA C A T T T T G T G G A CC G A A T A T G A T C C A A C A A T A
    T
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones, Secuencias conservadas 28 GAC GAT ASP ASP
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    300 310 170 180 190


    A G A A C A A A T T A A A A G A G T T A A G G A C TCGACACAGCAGGTCAAGAGGAGTACAGT

    Tumour cell DNA Tumour cell DNA

    300 310 170 180 190

    A G A A C A A A T T A A A A G A G T T A A G G A C TCGACACAGCAGGTCGAGAGGAGTACAGT
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    Secuencias conservadas 61 CAA CGA GLIN ARG
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    430 440 450 550 560


    TTTATTGAAACATCAGCAAAGACAAGACAG TCAAAGACAAAGTGTGTAATTATGTAA

    Tumour cell DNA Tumour cell DNA

    430 440 450 550 560


    C AAAGACAAGACAG
    C
    T T T A T T G A A A C A T C AcC TCAAAGACAAAGTGTGTAATTATGTAA
    G
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    10 GCA CCA Ala PRO No presenta mutaciones, Secuencia conservada
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    240 250 260 510 520 530


    TTTCTTTGTGTATTTGCCATAAATAATACT GAAAAGATGAGCAAAGATGGTAAAAAGAAG

    Tumour cell DNA Tumour cell DNA

    240 250 260 510 520 530

    TTTCTTTGTGTATTTGCCATAAATAATACT GAAAAGATGAGCAAAGA GGTAAAAAGAAG


    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones Secuencias conservadas 10 GAT GAC ASP Asp
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    10 20 30 150 160 170

    ATGACTGAATATAAACTTGTGGTAGTTGGA GGAGAAACCTGTCTCTTGGATATTCTCGAC

    Tumour cell DNA Tumour cell DNA

    10 20 30 150 160 170

    ATGACTGAATATAAACTTGTGGTAGTTGGA GGAGAAACCTGTCTCTTGGATATTCTCGAC
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones, Secuencia conservada No presenta mutaciones, Secuencia conservada
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    70 80 90 380 390 400

    ATA CAG CTA ATT CAG AAT CAT TTT GT


    GGAC GACACAAAACAGGCTCAGGACTTAGCAAGA

    Tumour cell DNA Tumour cell DNA

    70 80 90 380 390 400

    A T A C A G C T A A T T C A G A A T C A T T T T G TG GA C GACACAAAACAGGCTCAGGACTTAGCAAGA
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones, Secuencias conservadas No presenta mutaciones, Secuencias conservadas
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    410 420 430 490 500 510

    AGTTATGGAATTCCTTTTATTGAAACATCA CGAGAAATTCGAAAACATAAAGAAAAGATG

    Tumour cell DNA Tumour cell DNA

    410 420 430 490 500 510

    AGTTATGGAATTCCTTTTATTGAAACATCA CGAGAAATTCGAAAACATAAAGAAAAGATG
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones, Secuencias conservadas No presenta mutaciones, Secuencias conservadas
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    460 470 480 320 330 340

    GGTGTTGATGATGCCTTCTATACATTAGTT TCTGAAGATGTACCTATGGTCCTAGTAGGA

    Tumour cell DNA Tumour cell DNA

    460 470 480 320 330 340

    GGTGTTGATGATGCCTTCTATACATTAGTT TCTGAAGATGTACCTATGGTCCTAGTAGGA
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones, Secuencias conservadas No presenta mutaciones, Secuencias conservadas
    Can you spot a cancer mutation?
    Below are traces of sequenced DNA displaying different regions of the KRAS gene. DNA sequence from a healthy cell is Key
    shown above that of a tumour cell. Using the key provided, write out the DNA sequence for each trace. Compare the C - Cytosine
    healthy and tumour sequences. If you find a difference, circle the letter(s) that have changed in the sequence and then G - Guanine
    complete the table below using the banner or gene sheet and the codon wheel provided. T - Thymine
    C G TA A - Adenine
    Healthy cell DNA Healthy cell DNA

    120 130 140 270 280 290

    GATTCCTACAGGAAGCAAGTAGTAATTGAT AAATCATTTGAAGATATTCACCATTATAG

    Tumour cell DNA Tumour cell DNA

    120 130 140 270 280 290

    GATTCCTACAGGAAGCAAGTAGTAATTGAT AAATCATTTGAAGATATTCACCATTATAG
    Amino Acid Healthy DNA Tumour DNA Healthy Tumour Amino Acid Healthy DNA Tumour DNA Healthy Tumour
    Number Sequence Sequence Amino acid Amino Acid Number Sequence Sequence Amino acid Amino Acid
    No presenta mutaciones, Secuencias conservadas No presenta mutaciones, Secuencias conservadas

    You might also like